Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,103,201	T→A	5.5%	C12S (TGC→AGC)	PP_5353 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,103,201	0	T	A	5.5%	292.5 / 3.2	91	C12S (TGC→AGC)	PP_5353	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (46/40);  new base A (2/3);  total (48/43)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CCTGCCAACTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTGCACCGG  >  NC_002947/6103053‑6103345                                                                                                                                                     |                                                                                                                                                 ccTGCCAACTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGctct                                                                                                                                                  >  7:227134/1‑149 (MQ=255)   tGCCAACTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGc                                                                                                                                                >  7:239718/1‑149 (MQ=255)       aaCTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCg                                                                                                                                            <  8:239718/149‑1 (MQ=255)        aCTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCgg                                                                                                                                           <  8:249185/149‑1 (MQ=255)         cTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGc                                                                                                                                          <  3:390393/149‑1 (MQ=255)         cTTTGTAACATCCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGc                                                                                                                                          <  7:305927/149‑1 (MQ=255)          tttGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGcc                                                                                                                                         >  1:298648/1‑149 (MQ=255)               aaCATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACg                                                                                                                                    >  2:302869/1‑149 (MQ=255)                  aTTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCt                                                                                                                                  >  5:210568/1‑148 (MQ=255)                  aTTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTg                                                                                                                                 <  1:137007/149‑1 (MQ=255)                   ttCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCt                                                                                                                                  >  5:25504/1‑147 (MQ=255)                    tCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAg                                                                                                                               >  4:382907/1‑149 (MQ=255)                      aaGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGc                                                                                                                                          >  3:218751/1‑136 (MQ=255)                      aaGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGc                                                                                                                                          <  4:218751/136‑1 (MQ=255)                      aaGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGc                                                                                                                              <  1:302869/148‑1 (MQ=255)                      aaGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCAGCGTCAGGCCGACGCAGAGcg                                                                                                                             <  1:358822/149‑2 (MQ=255)                       aGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGc                                                                                                                              <  7:380887/147‑1 (MQ=255)                       aGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGc                                                                                                                              >  8:380887/1‑147 (MQ=255)                       aGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCAGGCCGACGCAGAGcgc                                                                                                                            <  5:202318/149‑3 (MQ=255)                       aGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCAGCGTCAGGCCGACGCAGAGcga                                                                                                                            >  5:167655/1‑147 (MQ=255)                       aGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCAGCGTCAGGCCGACGCAGAGc                                                                                                                              <  6:167655/147‑1 (MQ=255)                        gCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTg                                                                                                                           >  8:197429/1‑149 (MQ=255)                        gCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCAGCGTCAGGCCGACGCAGAGc                                                                                                                              >  1:131517/1‑146 (MQ=255)                        gCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCAGCGTCAGGCCGACGCAGAGc                                                                                                                              <  2:131517/146‑1 (MQ=255)                          aaaaGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGa                                                                                                                         >  8:220813/1‑149 (MQ=255)                           aaaGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGAc                                                                                                                        <  3:382907/149‑1 (MQ=255)                            aaGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACg                                                                                                                       >  8:194164/1‑149 (MQ=255)                             aGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGc                                                                                                                      <  4:359108/149‑1 (MQ=255)                                  gAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAgcg                                                                                                                 >  2:331750/1‑149 (MQ=255)                                  gAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAgcg                                                                                                                 >  4:388294/1‑149 (MQ=255)                                  gAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAgcg                                                                                                                 >  8:332051/1‑149 (MQ=255)                                  gAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCAGGACGCCAgcg                                                                                                                 >  3:248582/1‑149 (MQ=255)                                   aaaCACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAgcgc                                                                                                                >  3:376471/1‑149 (MQ=255)                                   aaaCACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAgcgc                                                                                                                >  3:241139/1‑149 (MQ=255)                                     acacTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGcc                                                                                                               >  6:285474/1‑148 (MQ=255)                                         tATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGAGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCCGACGCTGa                                                                                                                                <  8:284969/127‑1 (MQ=255)                                         tATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGAGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCCGACGCTGa                                                                                                                                >  7:284969/1‑127 (MQ=255)                                           tGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGtt                                                                                                        <  1:331750/149‑1 (MQ=255)                                             tCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGttt                                                                                                       <  4:241139/148‑1 (MQ=255)                                              cGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCaa                                                                                                             <  8:119672/141‑1 (MQ=255)                                              cGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCaa                                                                                                             >  7:119672/1‑141 (MQ=255)                                               gCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTcgg                                                                                                    >  3:63105/1‑149 (MQ=255)                                                   gTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTcggcgg                                                                                                 <  5:285474/148‑1 (MQ=255)                                                      aTGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCggcggcggc                                                                                             <  3:142928/149‑1 (MQ=255)                                                        gtgCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCggcggcggc                                                                                             >  8:342259/1‑147 (MQ=255)                                                        gtgCCCGGGAACCTGTGCACCATGACGCGAGTGCAATCCACAACAGGGGATATCCAACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGCCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCggcggcggc                                                                                             >  6:28280/1‑147 (MQ=255)                                                         tgCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCggcggcggc                                                                                             >  5:331404/1‑146 (MQ=255)                                                         tgCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCaa                                                                                           <  2:298648/148‑1 (MQ=255)                                                           cccGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAaga                                                                                         <  6:25504/148‑1 (MQ=255)                                                             cGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCt                                                                                      >  2:124486/1‑149 (MQ=255)                                                               ggAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCg                                                                                    <  4:376471/149‑1 (MQ=255)                                                                 aaCCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCggg                                                                                  <  7:220813/149‑1 (MQ=255)                                                                    cTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTc                                                                               >  7:205069/1‑149 (MQ=255)                                                                       tgCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTcc                                                                              <  7:285452/147‑1 (MQ=255)                                                                         cACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCgcgc                                                                          <  8:227134/149‑1 (MQ=255)                                                                          aCCATGACGCGCGTGCAATCCACAACAGGGGATATCACACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGcc                                                                         >  6:145132/1‑149 (MQ=255)                                                                            cATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCTGCAAGAGCTCGGGCTCCGCGCCTa                                                                       <  2:224378/149‑1 (MQ=255)                                                                            cATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTa                                                                       >  4:160227/1‑149 (MQ=255)                                                                             aTGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCaa                                                                                                             <  8:270665/110‑1 (MQ=255)                                                                             aTGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTAAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCaa                                                                                                             >  7:270665/1‑110 (MQ=255)                                                                                 cgcgcgTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATccac                                                                  <  8:205069/149‑1 (MQ=255)                                                                                 cgcgcgTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATccac                                                                  <  7:194164/149‑1 (MQ=255)                                                                                        gCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGAccc                                                           >  4:66201/1‑149 (MQ=255)                                                                                          aaTCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGc                                                         >  6:334853/1‑149 (MQ=255)                                                                                             ccACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTTGGCGGCGGCAAGAGCTCGGGCTCCgcgc                                                                          <  5:105607/129‑1 (MQ=255)                                                                                             ccACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTTGGCGGCGGCAAGAGCTCGGGCTCCgcgc                                                                          >  6:105607/1‑129 (MQ=255)                                                                                              cacaACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCa                                                       >  2:369244/1‑147 (MQ=255)                                                                                              cacaACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCa                                                       <  1:369244/147‑1 (MQ=255)                                                                                               acaacaGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATccacca                                                                >  3:314477/1‑137 (MQ=255)                                                                                               acaacaGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATccacca                                                                <  4:314477/137‑1 (MQ=255)                                                                                               acaacaGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGc                                                    >  6:170639/1‑149 (MQ=255)                                                                                                 aacaGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGATTCGGCTCCGGCAAGAGCTCGGGCTCCGCGCATATCCACCAGACCCGCCAGGCTa                                                  >  5:401643/1‑149 (MQ=255)                                                                                                      gggATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAgg                                                     <  2:362031/141‑1 (MQ=255)                                                                                                      gggATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAgg                                                     >  1:362031/1‑141 (MQ=255)                                                                                                        gATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAAc                                           <  7:197429/149‑1 (MQ=255)                                                                                                             cccACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGc                                      >  8:15452/1‑149 (MQ=255)                                                                                                                     tGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTTACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCtgctgc                               <  3:160227/148‑1 (MQ=255)                                                                                                                       cAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCcgc                            >  5:93721/1‑149 (MQ=255)                                                                                                                        aaCGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGTGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCcgcg                           >  4:255825/1‑149 (MQ=255)                                                                                                                        aaCGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCcgcg                           >  8:236717/1‑149 (MQ=255)                                                                                                                        aaCGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCcgcg                           >  5:50864/1‑149 (MQ=255)                                                                                                                           gTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACg                                                >  6:306922/1‑125 (MQ=255)                                                                                                                           gTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACg                                                <  5:306922/125‑1 (MQ=255)                                                                                                                            ttttCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGa                       <  6:331404/149‑1 (MQ=255)                                                                                                                                       tCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGc            >  6:412979/1‑149 (MQ=255)                                                                                                                                         gCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGcc           <  7:332051/148‑1 (MQ=255)                                                                                                                                          cACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGt         <  1:271026/149‑1 (MQ=255)                                                                                                                                           aCTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTg        <  3:66201/149‑1 (MQ=255)                                                                                                                                           aCTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTg        <  1:124486/149‑1 (MQ=255)                                                                                                                                               gcgcTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTGCAcc    >  6:189910/1‑149 (MQ=255)                                                                                                                                                 gcTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTGCACCgg  <  5:334853/149‑1 (MQ=255)                                                                                                                                                 gcTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTGCACCgg  >  6:141142/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CCTGCCAACTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTGCACCGG  >  NC_002947/6103053‑6103345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑