Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,103,207	G→A	6.6%	G14S (GGC→AGC) ‡	PP_5353 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,103,207	0	G	A	6.6%	271.0 / 5.7	90	G14S (GGC→AGC) ‡	PP_5353	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (45/39);  new base A (2/4);  total (47/43)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01

AACTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTGCACCGGCTGCCGC  >  NC_002947/6103059‑6103352                                                                                                                                                     |                                                                                                                                                  aaCTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCg                                                                                                                                                   <  8:239718/149‑1 (MQ=255)  aCTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCgg                                                                                                                                                  <  8:249185/149‑1 (MQ=255)   cTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGc                                                                                                                                                 <  3:390393/149‑1 (MQ=255)   cTTTGTAACATCCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGc                                                                                                                                                 <  7:305927/149‑1 (MQ=255)    tttGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGcc                                                                                                                                                >  1:298648/1‑149 (MQ=255)         aaCATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACg                                                                                                                                           >  2:302869/1‑149 (MQ=255)            aTTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCt                                                                                                                                         >  5:210568/1‑148 (MQ=255)            aTTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTg                                                                                                                                        <  1:137007/149‑1 (MQ=255)             ttCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCt                                                                                                                                         >  5:25504/1‑147 (MQ=255)              tCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAg                                                                                                                                      >  4:382907/1‑149 (MQ=255)                aaGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGc                                                                                                                                                 >  3:218751/1‑136 (MQ=255)                aaGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGc                                                                                                                                                 <  4:218751/136‑1 (MQ=255)                aaGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGc                                                                                                                                     <  1:302869/148‑1 (MQ=255)                aaGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCAGCGTCAGGCCGACGCAGAGcg                                                                                                                                    <  1:358822/149‑2 (MQ=255)                 aGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGc                                                                                                                                     <  7:380887/147‑1 (MQ=255)                 aGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGc                                                                                                                                     >  8:380887/1‑147 (MQ=255)                 aGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCAGGCCGACGCAGAGcgc                                                                                                                                   <  5:202318/149‑3 (MQ=255)                 aGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCAGCGTCAGGCCGACGCAGAGcga                                                                                                                                   >  5:167655/1‑147 (MQ=255)                 aGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCAGCGTCAGGCCGACGCAGAGc                                                                                                                                     <  6:167655/147‑1 (MQ=255)                  gCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTg                                                                                                                                  >  8:197429/1‑149 (MQ=255)                  gCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCAGCGTCAGGCCGACGCAGAGc                                                                                                                                     >  1:131517/1‑146 (MQ=255)                  gCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCAGCGTCAGGCCGACGCAGAGc                                                                                                                                     <  2:131517/146‑1 (MQ=255)                    aaaaGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGa                                                                                                                                >  8:220813/1‑149 (MQ=255)                     aaaGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGAc                                                                                                                               <  3:382907/149‑1 (MQ=255)                      aaGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACg                                                                                                                              >  8:194164/1‑149 (MQ=255)                       aGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGc                                                                                                                             <  4:359108/149‑1 (MQ=255)                            gAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAgcg                                                                                                                        >  2:331750/1‑149 (MQ=255)                            gAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAgcg                                                                                                                        >  8:332051/1‑149 (MQ=255)                            gAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAgcg                                                                                                                        >  4:388294/1‑149 (MQ=255)                            gAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCAGGACGCCAgcg                                                                                                                        >  3:248582/1‑149 (MQ=255)                             aaaCACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAgcgc                                                                                                                       >  3:376471/1‑149 (MQ=255)                             aaaCACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAgcgc                                                                                                                       >  3:241139/1‑149 (MQ=255)                               acacTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGcc                                                                                                                      >  6:285474/1‑148 (MQ=255)                                   tATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGAGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCCGACGCTGa                                                                                                                                       >  7:284969/1‑127 (MQ=255)                                   tATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGAGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCCGACGCTGa                                                                                                                                       <  8:284969/127‑1 (MQ=255)                                     tGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGtt                                                                                                               <  1:331750/149‑1 (MQ=255)                                       tCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGttt                                                                                                              <  4:241139/148‑1 (MQ=255)                                        cGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCaa                                                                                                                    <  8:119672/141‑1 (MQ=255)                                        cGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCaa                                                                                                                    >  7:119672/1‑141 (MQ=255)                                         gCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTcgg                                                                                                           >  3:63105/1‑149 (MQ=255)                                             gTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTcggcgg                                                                                                        <  5:285474/148‑1 (MQ=255)                                                aTGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCggcggcggc                                                                                                    <  3:142928/149‑1 (MQ=255)                                                  gtgCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCggcggcggc                                                                                                    >  8:342259/1‑147 (MQ=255)                                                  gtgCCCGGGAACCTGTGCACCATGACGCGAGTGCAATCCACAACAGGGGATATCCAACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGCCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCggcggcggc                                                                                                    >  6:28280/1‑147 (MQ=255)                                                   tgCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCggcggcggc                                                                                                    >  5:331404/1‑146 (MQ=255)                                                   tgCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCaa                                                                                                  <  2:298648/148‑1 (MQ=255)                                                     cccGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAaga                                                                                                <  6:25504/148‑1 (MQ=255)                                                       cGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCt                                                                                             >  2:124486/1‑149 (MQ=255)                                                         ggAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCg                                                                                           <  4:376471/149‑1 (MQ=255)                                                           aaCCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCggg                                                                                         <  7:220813/149‑1 (MQ=255)                                                              cTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTc                                                                                      >  7:205069/1‑149 (MQ=255)                                                                 tgCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTcc                                                                                     <  7:285452/147‑1 (MQ=255)                                                                   cACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCgcgc                                                                                 <  8:227134/149‑1 (MQ=255)                                                                    aCCATGACGCGCGTGCAATCCACAACAGGGGATATCACACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGcc                                                                                >  6:145132/1‑149 (MQ=255)                                                                      cATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCTGCAAGAGCTCGGGCTCCGCGCCTa                                                                              <  2:224378/149‑1 (MQ=255)                                                                      cATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTa                                                                              >  4:160227/1‑149 (MQ=255)                                                                       aTGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCaa                                                                                                                    <  8:270665/110‑1 (MQ=255)                                                                       aTGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTAAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCaa                                                                                                                    >  7:270665/1‑110 (MQ=255)                                                                           cgcgcgTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATccac                                                                         <  7:194164/149‑1 (MQ=255)                                                                           cgcgcgTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATccac                                                                         <  8:205069/149‑1 (MQ=255)                                                                                  gCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGAccc                                                                  >  4:66201/1‑149 (MQ=255)                                                                                    aaTCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGc                                                                >  6:334853/1‑149 (MQ=255)                                                                                       ccACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTTGGCGGCGGCAAGAGCTCGGGCTCCgcgc                                                                                 >  6:105607/1‑129 (MQ=255)                                                                                       ccACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTTGGCGGCGGCAAGAGCTCGGGCTCCgcgc                                                                                 <  5:105607/129‑1 (MQ=255)                                                                                        cacaACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCa                                                              <  1:369244/147‑1 (MQ=255)                                                                                        cacaACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCa                                                              >  2:369244/1‑147 (MQ=255)                                                                                         acaacaGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATccacca                                                                       <  4:314477/137‑1 (MQ=255)                                                                                         acaacaGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATccacca                                                                       >  3:314477/1‑137 (MQ=255)                                                                                         acaacaGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGc                                                           >  6:170639/1‑149 (MQ=255)                                                                                           aacaGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGATTCGGCTCCGGCAAGAGCTCGGGCTCCGCGCATATCCACCAGACCCGCCAGGCTa                                                         >  5:401643/1‑149 (MQ=255)                                                                                                gggATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAgg                                                            <  2:362031/141‑1 (MQ=255)                                                                                                gggATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAgg                                                            >  1:362031/1‑141 (MQ=255)                                                                                                  gATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAAc                                                  <  7:197429/149‑1 (MQ=255)                                                                                                       cccACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGc                                             >  8:15452/1‑149 (MQ=255)                                                                                                               tGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTTACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCtgctgc                                      <  3:160227/148‑1 (MQ=255)                                                                                                                 cAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCcgc                                   >  5:93721/1‑149 (MQ=255)                                                                                                                  aaCGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGTGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCcgcg                                  >  4:255825/1‑149 (MQ=255)                                                                                                                  aaCGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCcgcg                                  >  8:236717/1‑149 (MQ=255)                                                                                                                  aaCGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCcgcg                                  >  5:50864/1‑149 (MQ=255)                                                                                                                     gTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACg                                                       >  6:306922/1‑125 (MQ=255)                                                                                                                     gTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACg                                                       <  5:306922/125‑1 (MQ=255)                                                                                                                      ttttCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGa                              <  6:331404/149‑1 (MQ=255)                                                                                                                                 tCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGc                   >  6:412979/1‑149 (MQ=255)                                                                                                                                   gCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGcc                  <  7:332051/148‑1 (MQ=255)                                                                                                                                    cACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGt                <  1:271026/149‑1 (MQ=255)                                                                                                                                     aCTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTg               <  3:66201/149‑1 (MQ=255)                                                                                                                                     aCTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTg               <  1:124486/149‑1 (MQ=255)                                                                                                                                         gcgcTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTGCAcc           >  6:189910/1‑149 (MQ=255)                                                                                                                                           gcTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTGCACCgg         <  5:334853/149‑1 (MQ=255)                                                                                                                                           gcTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTGCACCgg         >  6:141142/1‑149 (MQ=255)                                                                                                                                                 cGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTGCACCGGCTgc     <  7:342259/147‑1 (MQ=255)                                                                                                                                                  gTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTGCACCGGCTgccgc  <  4:63105/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  AACTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACGCCAACCACGCCTGCTGCCGCGCCGACCGTACCTGGCCGTGCACCGGCTGCCGC  >  NC_002947/6103059‑6103352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑