Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,129,170	C→T	9.7%	intergenic (+32/+35)	PP_5376 → / ← PP_5377	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,129,170	0	C	T	9.7%	169.2 / 5.7	61	intergenic (+32/+35)	PP_5376/PP_5377	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/30);  new base T (3/3);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCG  >  NC_002947/6129024‑6129314                                                                                                                                                    |                                                                                                                                                 gACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCtt                                                                                                                                                <  4:156802/149‑1 (MQ=255)   cccGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTaa                                                                                                                                              <  5:250122/149‑1 (MQ=255)        ggAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAgcg                                                                                                                                         >  2:52044/1‑149 (MQ=255)              tgtgACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGCAAACGGCGCTTTTAAGGCGCCGttt                                                                                                                                   >  8:119123/1‑149 (MQ=255)                  aCGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGt                                                                                                                               <  1:86634/149‑1 (MQ=39)                   cGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGtt                                                                                                                              <  7:119123/149‑1 (MQ=39)                    gTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGcc                                                                                                                                                  >  4:50535/1‑128 (MQ=255)                    gTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGcc                                                                                                                                                  <  3:50535/128‑1 (MQ=255)                     tGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTgg                                                                                                                            <  8:281342/149‑1 (MQ=255)                         gCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGttt                                                                                                                                   <  4:306467/138‑1 (MQ=255)                         gCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGttt                                                                                                                                   >  3:306467/1‑138 (MQ=255)                            tGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACg                                                                                                                     >  8:104899/1‑149 (MQ=255)                               gcAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTg                                                                                                                  <  7:249733/149‑1 (MQ=255)                                 aaaTGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTggt                                                                                                                >  5:154040/1‑148 (MQ=255)                                           tGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCt                                                                                                      <  2:77346/149‑1 (MQ=255)                                           tGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCt                                                                                                      <  7:305238/149‑1 (MQ=255)                                            gAAGACTCGACAGGCAAGCTCAACATTCTCGAATTCCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTg                                                                                                     >  1:60068/1‑149 (MQ=255)                                            gAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATTCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTTGGTCAGTGGCCt                                                                                                      >  1:304585/1‑149 (MQ=255)                                             aaGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGt                                                                                                                   <  1:399116/134‑1 (MQ=255)                                             aaGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGt                                                                                                                   >  2:399116/1‑134 (MQ=255)                                              aGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGa                                                                                                   <  2:235748/149‑1 (MQ=255)                                                aCTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATa                                                                                                 >  1:281595/1‑149 (MQ=255)                                                    gACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATActct                                                                                             >  8:153763/1‑149 (MQ=255)                                                       aGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGc                                                                                           >  5:51669/1‑148 (MQ=255)                                                         gCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGCAAACGGCGCTTTTAAGGCGcc                                                                                                                                       <  5:415385/102‑1 (MQ=39)                                                         gCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGCAAACGGCGCTTTTAAGGCGcc                                                                                                                                       >  6:415385/1‑102 (MQ=39)                                                         gCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAAc                                                                                        <  3:63374/149‑1 (MQ=255)                                                         gCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAAc                                                                                        >  4:292064/1‑149 (MQ=255)                                                                 aaCATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTc                                                                                  <  2:206140/147‑1 (MQ=255)                                                                   cATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCaa                                                                                         >  5:393741/1‑138 (MQ=255)                                                                   cATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCaa                                                                                         <  6:393741/138‑1 (MQ=255)                                                                      tctcGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGt                                                                           >  8:262691/1‑149 (MQ=255)                                                                        tcGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGt                                                                           >  6:263171/1‑147 (MQ=255)                                                                        tcGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTcc                                                                         >  6:267838/1‑149 (MQ=255)                                                                               ccGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCtt                                                                   <  5:46914/148‑1 (MQ=255)                                                                                cgcgCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCttgt                                                                 >  1:390529/1‑149 (MQ=255)                                                                                 gcgcAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCttgtt                                                                <  1:52044/149‑1 (MQ=255)                                                                                 gcgcAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCttgtt                                                                <  7:153763/149‑1 (MQ=255)                                                                                   gcAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTga                                                              <  3:104126/149‑1 (MQ=255)                                                                                       cTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGAcc                                                          <  5:263171/149‑1 (MQ=255)                                                                                          gCA‑TCAATTGCCGCGGCAAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGAt                                                       <  2:345089/149‑1 (MQ=255)                                                                                              tCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGAc                                                    >  5:168824/1‑149 (MQ=255)                                                                                                aaTTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGAACGATGACCt                                                  >  3:109441/1‑149 (MQ=255)                                                                                                     ccGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTAt                                             <  7:104899/149‑1 (MQ=255)                                                                                                           ccAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATga                                       <  2:304585/149‑1 (MQ=255)                                                                                                             aaaaTTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATgaga                                     <  2:281595/149‑1 (MQ=255)                                                                                                                aTTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGac                                  <  5:267838/149‑1 (MQ=255)                                                                                                                       tctGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGAc                                                    >  4:101951/1‑124 (MQ=255)                                                                                                                       tctGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATc                            >  8:57544/1‑148 (MQ=255)                                                                                                                                     aaaCGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATgaga                                     <  2:249486/125‑1 (MQ=255)                                                                                                                                     aaaCGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATgaga                                     >  1:249486/1‑125 (MQ=255)                                                                                                                                     aaaCGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACAcc                               >  2:390756/1‑131 (MQ=255)                                                                                                                                     aaaCGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACAcc                               <  1:390756/131‑1 (MQ=255)                                                                                                                                      aaCGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGga            <  3:292064/149‑1 (MQ=255)                                                                                                                                       aCGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATAc                 >  2:146446/1‑143 (MQ=255)                                                                                                                                       aCGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATAc                 <  1:146446/143‑1 (MQ=255)                                                                                                                                        cGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGgaga          <  2:60068/149‑1 (MQ=255)                                                                                                                                         gAAAACGGCGCCTTAAATGCGCAGTTTGCGTTGGGAAAACGGTTGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCttgttg                                                               <  6:272594/95‑1 (MQ=255)                                                                                                                                         gAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGagag         >  6:273739/1‑149 (MQ=255)                                                                                                                                         gAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCACGTCCGCTCttgttg                                                               >  5:272594/1‑95 (MQ=255)                                                                                                                                          caaaCGGCGCTTTTAAGGCGCCGTTTTCGTTTGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGc        >  7:219813/2‑149 (MQ=39)                                                                                                                                            aaCGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCt      <  7:262691/149‑1 (MQ=255)                                                                                                                                                gcgcCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCg  <  4:109441/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCA‑TCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCG  >  NC_002947/6129024‑6129314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑