Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 591,082 T→C 8.4% V110A (GTG→GCG)  PP_0501 → NAD‑dependent epimerase/dehydratase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947591,0820TC 8.4% 233.0 / 9.5 85V110A (GTG→GCG) PP_0501NAD‑dependent epimerase/dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base T (38/39);  new base C (3/4);  total (41/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

CAGGGCTGGTCACTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCAGTGAGCAGTACCTGGACTTC  >  NC_002947/590935‑591230
                                                                                                                                                   |                                                                                                                                                     
cAGGGCTGGTCACTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGt                                                                                                                                                       <  6:199758/148‑1 (MQ=255)
 aGGGCTGGTCACTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTg                                                                                                                                                      >  3:73666/1‑148 (MQ=255)
  gggCTGGTCACTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTAGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCa                                                                                                                                                    >  5:133290/1‑149 (MQ=255)
   ggCTGGTCACTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCAt                                                                                                                                                   >  4:111350/1‑149 (MQ=255)
    gCTGGTCACTCAGGCTGCGGCTGGCTGCGGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATGGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATg                                                                                                                                                  <  1:43860/149‑1 (MQ=255)
    gCTGGTCACTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATg                                                                                                                                                  <  8:342977/149‑1 (MQ=255)
    gCTGGTCACTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATg                                                                                                                                                  <  3:364636/149‑1 (MQ=255)
      tGGTCACTCAGGCTGCGGCTGGCTGCCGTGATGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGc                                                                                                                                                <  8:136456/149‑1 (MQ=255)
        gTCACTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGt                                                                                                                                              >  5:31336/1‑149 (MQ=255)
        gTCACTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCACGCGCATGGctt                                                                                                                                              <  7:822/149‑3 (MQ=255)
         tCACTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTg                                                                                                                                             <  6:419478/149‑1 (MQ=255)
         tCACTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCACGCGCATGGcttc                                                                                                                                             <  5:419675/149‑4 (MQ=255)
         tCACTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCACGCGCATGGcttc                                                                                                                                             <  2:180100/149‑4 (MQ=255)
            cTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTgcgc                                                                                                                                          >  8:203647/1‑149 (MQ=255)
            cTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGACGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTgcgc                                                                                                                                          >  5:117448/1‑149 (MQ=255)
               aGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCgg                                                                                                                                       <  1:179025/149‑1 (MQ=255)
                ggCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTGCAGGCATCGGTCGAAGACCCGGTGCGTACGCACCAGAGTAACTTCATCGGTACCGTCAACGTTTGCGATGCCATGCGCGTGCATGGCGTGCGCCggg                                                                                                                                      <  1:317180/149‑1 (MQ=255)
                 gCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGt                                                                                                                                     >  3:187869/1‑149 (MQ=255)
                     cGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGctc                                                                                                                                 <  3:375627/149‑1 (MQ=255)
                      ggctggctGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCgg                                                                                                                                       >  4:325740/1‑142 (MQ=255)
                      ggctggctGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCgg                                                                                                                                       <  3:325740/142‑1 (MQ=255)
                        ctggctgCCGTGCGGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCTGTCATCGGTCGAAGACCGGGTGCGTACCCACCTGAGTTACTTCATCGGTACCCTCTCCGTTTGCGAAGCGATGGGCGGTCATGGCGTGCGCCGGGTGCTCTTc                                                                                                                              <  2:395734/149‑1 (MQ=255)
                             tgCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGATCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAGGCCAAGGGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTc                                                                                                                         >  1:339549/1‑149 (MQ=255)
                             tgCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTc                                                                                                                         >  2:356449/1‑149 (MQ=255)
                                cGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCcag                                                                                                                      >  8:192672/1‑149 (MQ=255)
                                 gTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCcagc                                                                                                                     <  7:333784/149‑1 (MQ=255)
                                         gtgCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGgt                                                                                                             >  1:224901/1‑149 (MQ=255)
                                         gtgCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGgt                                                                                                             >  2:109379/1‑149 (MQ=255)
                                            cATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTa                                                                                                          <  6:31336/149‑1 (MQ=255)
                                              tCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACg                                                                                                        >  4:194113/1‑149 (MQ=255)
                                                tGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTACTCTTCGCCTCcagcgcagc                                                                                                                >  5:58093/1‑139 (MQ=255)
                                                tGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTACTCTTCGCCTCcagcgcagc                                                                                                                <  6:58093/139‑1 (MQ=255)
                                                  gcggcgGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGTGCCgg                                                                                                                                       >  7:359689/1‑114 (MQ=255)
                                                  gcggcgGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGTGCCgg                                                                                                                                       <  8:359689/114‑1 (MQ=255)
                                                  gcggcgGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACgg                                                                                                       <  1:356449/146‑1 (MQ=255)
                                                  gcggcgGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACg                                                                                                        <  1:41504/145‑1 (MQ=255)
                                                  gcggcgGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGcaa                                                                                                    <  4:150502/149‑1 (MQ=255)
                                                       ggTAGCGTCGGTTCAGGCATAGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATg                                                                                               >  2:260371/1‑149 (MQ=255)
                                                        gTAGCGTCGGTTCAGGCATCGGACGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATgg                                                                                              <  6:117448/149‑1 (MQ=255)
                                                           gCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTg                                                                                            >  4:66612/1‑148 (MQ=255)
                                                             gTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAg                                                                                         <  3:63628/149‑1 (MQ=255)
                                                              tCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAgg                                                                                        <  3:111350/149‑1 (MQ=255)
                                                               cGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAggg                                                                                       <  8:270923/149‑1 (MQ=255)
                                                                 gTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTg                                                                                                                                    <  8:299233/102‑1 (MQ=255)
                                                                 gTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTg                                                                                                                                    >  7:299233/1‑102 (MQ=255)
                                                                      ggCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGa                                                                                <  4:73666/149‑1 (MQ=255)
                                                                       gCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGACGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGAt                                                                               >  1:345625/1‑149 (MQ=255)
                                                                         aTCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAggg                                                                                       >  6:227423/1‑139 (MQ=255)
                                                                         aTCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAggg                                                                                       <  5:227423/139‑1 (MQ=255)
                                                                             gTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCg                                                                          >  7:25190/1‑148 (MQ=255)
                                                                                gAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGa                                                                      >  8:384828/1‑149 (MQ=255)
                                                                                      ccGGTGCGTACCCACGAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGGATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAAGGGTGAAGGGGAATGGATTTCGGCAGACACGc                                                                 >  6:220735/1‑148 (MQ=255)
                                                                                      ccGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGc                                                                 >  5:32928/1‑148 (MQ=255)
                                                                                                cccACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCg                                                      <  4:47010/149‑1 (MQ=255)
                                                                                                cccACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCg                                                      >  3:47010/1‑149 (MQ=255)
                                                                                                   accaGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGtt                                                    >  3:357154/1‑148 (MQ=255)
                                                                                                    ccaGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑a                                                 >  6:107242/1‑149 (MQ=255)
                                                                                                        agTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑Acccc                                             <  7:203647/149‑1 (MQ=255)
                                                                                                          tAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCt                                            >  5:68941/1‑148 (MQ=255)
                                                                                                          tAACTTCATCGGTACCCCCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCtt                                           <  7:363401/149‑1 (MQ=255)
                                                                                                          tAACTTCATCGGTACCCCCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCt                                            >  8:363401/1‑148 (MQ=255)
                                                                                                               tCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGcc                                      >  1:391870/1‑149 (MQ=255)
                                                                                                                cATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGc                                       <  3:53426/147‑1 (MQ=255)
                                                                                                                  tCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTg                                   <  6:68941/149‑1 (MQ=255)
                                                                                                                       aCCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGAc                                >  7:241865/1‑147 (MQ=255)
                                                                                                                             aaCGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTg                                   <  8:260237/138‑1 (MQ=255)
                                                                                                                             aaCGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTg                                   >  7:260237/1‑138 (MQ=255)
                                                                                                                             aaCGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTgg                         <  8:25190/148‑1 (MQ=255)
                                                                                                                             aaCGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTgg                         <  7:363996/148‑1 (MQ=255)
                                                                                                                             aaCGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGc                        >  7:347398/1‑149 (MQ=255)
                                                                                                                             aaCGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGc                        >  4:234911/1‑149 (MQ=255)
                                                                                                                             aaCGTGTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGc                        <  4:271549/149‑1 (MQ=255)
                                                                                                                               cGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCa                      <  7:192672/149‑1 (MQ=255)
                                                                                                                                gTTTGCGAAGCCATGCGCGTGCATGGCGAGCGCCGGGTGCTCTTCGCCTCCAGCGCATCGGTGTACGGCTACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCGCTTATGCCGTGGACAAGCTGGCCAg                     >  1:228961/1‑149 (MQ=255)
                                                                                                                                     cGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTGAACCCCTTATGCCGTGGACAAGCTGGCCAGTGAg                 <  1:260371/149‑1 (MQ=255)
                                                                                                                                     cGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCAGTGAg                 <  5:107242/148‑1 (MQ=255)
                                                                                                                                     cGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCAGTGAGc                <  7:384828/149‑1 (MQ=255)
                                                                                                                                      gAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCAGTGAGCa               <  8:347398/149‑1 (MQ=255)
                                                                                                                                      cacgCCATGCACGCGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCAGTGAg                 >  2:134317/4‑147 (MQ=255)
                                                                                                                                       acgCCATGCACGCGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCAGTGAGCAg              >  6:353745/3‑149 (MQ=255)
                                                                                                                                        cgCCATGCACGCGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCAGTGAGCAg              >  3:342892/2‑148 (MQ=255)
                                                                                                                                        aGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCAGTGAGCAGt             <  1:109379/149‑1 (MQ=255)
                                                                                                                                        aGCCATGCGCGTGCATGGCGTGCGCCGGGTCCTCTTCGACTCCAGCGCAGCGGTGTACGGCAACAATGGTGACGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCAGTGAGCAGt             <  6:133290/149‑1 (MQ=255)
                                                                                                                                             tGCACGCGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCAGTGAGCAGTACCTg        <  4:342892/149‑1 (MQ=255)
                                                                                                                                                gcgTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCAGTGAGCAGTACCTGGAc     >  4:355003/1‑149 (MQ=255)
                                                                                                                                                gcgTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCAGTGAGCAGTACCTGGAc     >  2:139537/1‑149 (MQ=255)
                                                                                                                                                   tGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCAGTGAGCAGTACCTGGACTTc  <  5:220735/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CAGGGCTGGTCACTCAGGCTGCGGCTGGCTGCCGTGCTGTGGTGCATCTGGCGGCGGTAGCGTCGGTTCAGGCATCGGTCGAAGACCCGGTGCGTACCCACCAGAGTAACTTCATCGGTACCCTCAACGTTTGCGAAGCCATGCGCGTGCATGGCGTGCGCCGGGTGCTCTTCGCCTCCAGCGCAGCGGTGTACGGCAACAATGGTGAAGGGGAATCGATTTCCGAAGACACGCCAAAGGCGCCGTTG‑ACCCCTTATGCCGTGGACAAGCTGGCCAGTGAGCAGTACCTGGACTTC  >  NC_002947/590935‑591230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑