Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	648,287	A→C	11.5%	intergenic (+56/+35)	acoR → / ← accC	acetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	648,287	0	A	C	11.5%	144.8 / 7.4	58	intergenic (+56/+35)	acoR/accC	acetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base A (19/32);  new base C (3/4);  total (22/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTC  >  NC_002947/648149‑648435                                                                                                                                           |                                                                                                                                                     cACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGGCGCGGCGCGGCCCCCGAATGTGGGAGGGAATTAAAAAACCCCGCCCAcgggcggg                                                                                                                                            >  2:145239/1‑149 (MQ=255)  actggCTTTGTGCACTTCGCGGGCTACGCTTTATCGGCGATTGCAGGAGCACCGCATCGACATGCCCCGGATGAAGGGGTAGAGCCCTGGCGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAGAACCCCGGCCCTGTGCCgggg                                                                                                                                           <  4:257380/146‑1 (MQ=255)  aCGGGCTTTGGGCATTTCGCGGGCCACGCTTTATCGGCGAGGGCATCAGCACCGCAGCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCCCTGTGCCgggg                                                                                                                                           <  4:189128/149‑1 (MQ=255)    gggCTTTGGGATTTTCGCGGGGGACGGTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCCCTGTGCCGGGGtt                                                                                                                                         <  2:286408/149‑1 (MQ=255)     ggCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGCGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTc                                                                                                                                        <  7:275247/149‑1 (MQ=255)           gggCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTTGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTa                                                                                                                                   <  4:311789/148‑1 (MQ=255)           gggCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTa                                                                                                                                   <  7:209121/148‑1 (MQ=255)             gCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACAt                                                                                                                                <  4:351221/149‑1 (MQ=255)               tgttCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATca                                                                                                                              <  1:145239/147‑1 (MQ=255)                    gcgGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTa                                                                                                                         <  2:296260/149‑1 (MQ=255)                      gggCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTaca                                                                                                                       <  7:278733/149‑1 (MQ=255)                         cGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGa                                                                                                                    <  6:101659/149‑1 (MQ=255)                          gACGCTTTATCGGCGAGTGCATGAGCACCGGATGGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCGGGGGTTCTGGTACATCACACTACACGAt                                                                                                                   <  7:64894/149‑1 (MQ=255)                               tttATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGt                                                                                                                                    <  3:151770/127‑1 (MQ=255)                                 tATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTcc                                                                                                             <  2:137984/148‑1 (MQ=255)                                  aTCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCt                                                                                                            <  7:262184/148‑1 (MQ=255)                                   tCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTgg                                                                                                          <  4:23258/149‑1 (MQ=255)                                   tCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTgg                                                                                                          <  7:74823/149‑1 (MQ=255)                                     ggCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTCGGGGCCCGCCCCGGCCCCCCAATAAGGGAGGCAATAAAAAAAACCCCGACACGAGCGGGGTTCTGGGAAAAAACACCACACCATCACAGCTggat                                                                                                        >  8:203967/1‑147 (MQ=255)                                      gCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTg                                                                                                       <  4:98361/149‑1 (MQ=255)                                             cATGAGCACCGCATCGACATGCCGCGGATGAAGGGCTAGAGCCCTGGGGCCGCGACGCCGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCCCAGGGCCGGGGTTCTGGTACATCACACTACACCATCACTCCTGGTTGGCCAGtt                                                                                                >  1:266830/1‑149 (MQ=255)                                                gAGCACGGCATCGACATGCCGCGGATGAAGGGGTAGACCCCTGGGGCCGCGACGCGGCCCCAGATTCAGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTtgt                                                                                             <  7:145538/149‑1 (MQ=255)                                                  gCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGc                                                                                           <  4:121333/149‑1 (MQ=255)                                                   cACCGCATCGACATGCCGCGGATGAAGGGTTATAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTtgtg                                                                                            <  5:133381/147‑1 (MQ=255)                                                   cACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCt                                                                                          <  7:182847/149‑1 (MQ=255)                                                   cACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCCCCCCGGCCCCACAATATGGCAGGCAATAAAAAAACCCGGCACAGGGCCCGGGTTGTGGTACATCACACTAAACCAAAACACCAGGTTGGGCCGTTtgtg                                                                                            >  6:133381/1‑147 (MQ=255)                                                                tGCCGCGGATGAATGGGTATAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGtt                                                                                                <  3:22435/130‑1 (MQ=255)                                                                tGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAAt                                                                             <  4:87412/149‑1 (MQ=255)                                                                             ggggTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGt                                                                                                         <  5:80737/108‑1 (MQ=255)                                                                                 ccagaatcTGGGGCCGCGTCGCGGGCCCAGATTGTGGCAGGCAATAAAAAACCCCGGCACAGGGGCGGGGTTCTGGTTCATCACACTACACGATCACTCCTGGTTGGGCAGGTTGTGCTGGAGGTAGTGAATGTTGACGCCGCCTTTg                                                             >  1:166654/8‑148 (MQ=255)                                                                                     gCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGaa                                                        <  1:55747/149‑1 (MQ=255)                                                                                     gCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGaa                                                        <  1:61672/149‑1 (MQ=255)                                                                                      cccTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGc                                                                                           <  2:20644/113‑1 (MQ=255)                                                                                      cccTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGc                                                                                           >  1:20644/1‑113 (MQ=255)                                                                                      cccTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAg                                                       >  3:291613/1‑149 (MQ=255)                                                                                              ccGCGACGCGGCCCGAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTtcatc                                               >  7:346861/1‑149 (MQ=255)                                                                                                gcgACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTAGCAGAAGCCTTCATCAc                                             >  5:168291/1‑149 (MQ=255)                                                                                                     gcgGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCAcc                                        >  6:249489/1‑149 (MQ=255)                                                                                                       ggCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAg                                      <  8:346861/149‑1 (MQ=255)                                                                                                        gCCCCAGGTTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGc                                                            <  1:226808/126‑1 (MQ=255)                                                                                                        gCCCCAGGTTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGc                                                            >  2:226808/1‑126 (MQ=255)                                                                                                          cccAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTc                                   >  6:181030/1‑149 (MQ=255)                                                                                                            cAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCa                                          >  1:362755/1‑140 (MQ=255)                                                                                                            cAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTcgc                                 >  4:27779/1‑149 (MQ=255)                                                                                                                ttCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGt                              <  2:166654/148‑1 (MQ=255)                                                                                                                   tGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAg                          >  1:72216/1‑149 (MQ=255)                                                                                                                         gcaATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCg                        <  5:190300/145‑1 (MQ=255)                                                                                                                         gcaATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCg                        >  6:190300/1‑145 (MQ=255)                                                                                                                           aaTAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGt                  >  4:378816/1‑149 (MQ=255)                                                                                                                            aTAAAAAACCACGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGtt                 >  1:22846/1‑149 (MQ=255)                                                                                                                             tAAAAACCCCCGGCACCGGGCCGGGGTTCTGGTACATCACACTACACGAGCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTg                <  4:291613/149‑1 (MQ=255)                                                                                                                              aaaaaaCCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGGCTTCATCACGCACAAGGTCGCGGTGCAGCGGGTGGTTgg               >  4:221082/1‑149 (MQ=255)                                                                                                                                aaaaCCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTc             <  2:22846/149‑1 (MQ=255)                                                                                                                                 aaaCCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCt            >  4:221344/1‑149 (MQ=255)                                                                                                                                   aCCCCGGCCCTGTGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTg                <  2:166261/143‑1 (MQ=255)                                                                                                                                   aCCCCGGCCCTGTGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTg                >  1:166261/1‑143 (MQ=255)                                                                                                                                     cccGGCACAGGGCCGGGTTTCTTGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGAt        <  7:203967/149‑1 (MQ=255)                                                                                                                                     cccGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGAt        >  7:65926/1‑149 (MQ=255)                                                                                                                                           acaGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGTAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTc  >  8:324020/1‑149 (MQ=255)                                                                                                                                           acaGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTc  <  2:72216/149‑1 (MQ=255)                                                                                                                                           acaGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATAACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTc  >  2:397849/1‑149 (MQ=255)                                                                                                                                           |                                                                                                                                                     CACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTC  >  NC_002947/648149‑648435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑