| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 697,481 | G→A | 7.4% | intergenic (+408/‑343) | mmsA‑I → / → PP_16SD | methylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 697,481 | 0 | G | A | 7.4% | 220.8 / 6.1 | 81 | intergenic (+408/‑343) | mmsA‑I/PP_16SD | methylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (33/42); new base A (2/4); total (35/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01 | |||||||||||
CCCTCTTGAATCCCCTTATAATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGA > NC_002947/697335‑697627 | cccTCTTGAATCCCCTTATACTGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCt < 8:51197/149‑1 (MQ=255) tctTGAATCCCCTTATAATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCACttg > 5:247949/1‑147 (MQ=255) ctggAATCCCCTTATAATGCGCCCCACTTCCAGCGACAACGGAACGACATACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTcc < 5:235790/145‑1 (MQ=255) ctTGAATCCCCTTATAATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAAAGAGTTAGACAATTCTGAAGGTCCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑CTGGAGATGAAGGTTGACAGCGCTAcc > 6:235790/1‑148 (MQ=25) ttGAATCCCCTTATAATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGGGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTcc < 6:242904/147‑1 (MQ=255) ccTTATAATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCACTTGGAAGCGCTGtc < 6:247949/149‑2 (MQ=255) ataATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCACTTGGAAGCGCTGtcaac < 5:409053/148‑5 (MQ=21) ataATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCACTTGGAAGCGCTGtcaa < 5:55793/147‑4 (MQ=25) cccACTTCCAGCGACAACGTAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGAtt < 5:63585/141‑1 (MQ=255) cccACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGAtt > 6:63585/1‑141 (MQ=255) cACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGc < 7:154780/149‑1 (MQ=255) aCTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGGTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTa > 6:157110/1‑133 (MQ=255) aCTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTa < 5:157110/133‑1 (MQ=255) ttCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGATTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTAc < 4:21786/149‑1 (MQ=255) aGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACgaga < 8:206459/149‑1 (MQ=255) gCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACgagag < 5:277789/149‑1 (MQ=255) gCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACga > 3:302083/1‑146 (MQ=255) gCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACga > 3:296740/1‑146 (MQ=255) gACAACGGAACGACAAATTCCTTGAAATTCAATGAGTTAGACAATACTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGAt < 2:186256/149‑1 (MQ=255) aCAAAGGAACGAAAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATc < 4:225062/149‑1 (MQ=255) aaCGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCg > 4:336715/1‑148 (MQ=255) ggAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGc < 3:208115/149‑1 (MQ=255) gACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGc > 3:194656/1‑144 (MQ=255) gACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGc < 4:194656/144‑1 (MQ=255) gACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGc > 6:416220/1‑148 (MQ=255) cAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGACTCCCGCTACGAGAGATCGCAGCGAGcc > 4:116261/1‑147 (MQ=255) cAAACTCCTTGAAATTCAATGAGTTAGACAAATCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGcc > 2:77121/1‑147 (MQ=255) aaaCTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAg > 7:254225/1‑149 (MQ=255) tCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGtt > 2:31432/1‑149 (MQ=255) ttGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTg > 5:261400/1‑148 (MQ=255) tGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGAGTTGAGATGATGGTTGACAGCGCTTCCAAGTGATGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGttt > 1:120351/1‑148 (MQ=255) gAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGaa < 7:182183/148‑1 (MQ=255) aaaTTCAATGAGTTATAGAATTCTGAACGTGCTGTAAGGTCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGTTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGc < 1:75855/149‑1 (MQ=17) aTTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCt > 5:112113/1‑148 (MQ=255) aaTGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAAc < 4:302083/148‑1 (MQ=255) aaTGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAAc < 4:296740/148‑1 (MQ=255) aTGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGa < 8:254225/149‑1 (MQ=255) tgtgTTAGACTATTCTGAAGGTGATGTAAGGTCTACTTTCAAATGATCGTCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTTTATGATTCGCCTCCCGCTAAGAGAGATGCCAGCGAGCCAAGTGTTTGAAGCTAAACGAg < 1:34084/146‑1 (MQ=17) tGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAg > 5:336514/1‑149 (MQ=255) ttAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTtc < 2:334345/149‑1 (MQ=255) gACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTtctc < 5:416220/148‑1 (MQ=255) aCAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCg > 2:321211/1‑148 (MQ=255) cAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGc > 8:212214/1‑148 (MQ=255) cAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGATAAACGAGTTTCTCGc > 8:206455/1‑148 (MQ=255) aaTTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCa > 5:175502/1‑148 (MQ=255) aaTTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATCCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCa > 8:108247/1‑148 (MQ=255) aaGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCaaaaaa < 7:14499/146‑1 (MQ=255) aaGGGGCTGTAAGGCCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTCCTGTATGATTCGCCTACCGCTACGAGATATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACtt < 8:74262/149‑1 (MQ=14) ggTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATCCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCa < 7:108247/149‑1 (MQ=255) gCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACg > 1:361746/1‑89 (MQ=255) gCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACg < 2:361746/89‑1 (MQ=255) aGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACtt > 5:254737/1‑138 (MQ=40) aGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACtt < 6:254737/138‑1 (MQ=40) gggCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGc > 6:345462/1‑149 (MQ=39) cGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGa > 5:252055/1‑147 (MQ=39) cGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACCAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACa < 4:390589/149‑1 (MQ=255) gATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAg > 6:303736/1‑149 (MQ=39) aTCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAATCGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAgg > 7:291238/1‑149 (MQ=255) aTCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAgg < 6:261400/149‑1 (MQ=39) aaTGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGctct > 8:345830/1‑149 (MQ=37) aTCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAg < 5:303736/148‑1 (MQ=37) gCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGaaa < 5:345462/149‑1 (MQ=34) cAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGc < 6:336514/149‑1 (MQ=32) gCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAAAGCTTGACAGGCTCTGAGGAAAGCGt < 2:116698/149‑1 (MQ=17) cG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAATCGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTa < 8:291238/149‑1 (MQ=17) cG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTa < 1:185622/149‑1 (MQ=32) gTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGa < 2:325495/149‑1 (MQ=32) gagaTGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCGCTGAGGAAAGCGTAGAAt < 5:37372/148‑1 (MQ=17) gatgatGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATgcg > 5:252905/1‑149 (MQ=32) gatgGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATgcgcgc > 2:264631/1‑149 (MQ=32) gatgGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATgcgcgc > 8:49053/1‑149 (MQ=32) gTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCg < 1:77121/149‑1 (MQ=32) ttGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCTAAATAAACGCTTGACAGGCTCTGAGGaaa < 1:237528/129‑1 (MQ=17) ttGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGaaa > 2:237528/1‑129 (MQ=32) tGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGGTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAAc > 5:248865/1‑107 (MQ=17) tGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGCTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAAc < 6:248865/107‑1 (MQ=17) ataCAGCACTTGGAAGCGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGt > 4:143362/3‑149 (MQ=255) taCAGCACTTGGAAGCGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGtt < 3:143362/148‑1 (MQ=255) aCAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTg < 6:112113/149‑1 (MQ=32) cAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTga < 6:175502/149‑1 (MQ=32) cAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTga < 6:252905/149‑1 (MQ=32) gcgcTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTgaga > 8:32338/1‑149 (MQ=32) gcgcTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTgaga < 6:252055/149‑1 (MQ=32) | CCCTCTTGAATCCCCTTATAATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCG‑‑GTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGA > NC_002947/697335‑697627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |