Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 85,088 C→A 9.9% intergenic (+66/+64) aroE → / ← PP_0075 shikimate 5‑dehydrogenase/choline sulfate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294785,0880CA 9.9% 176.2 / 9.7 61intergenic (+66/+64)aroE/PP_0075shikimate 5‑dehydrogenase/choline sulfate transporter
Reads supporting (aligned to +/- strand):  ref base C (23/32);  new base A (2/4);  total (25/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-02

AAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGC  >  NC_002947/84940‑85233
                                                                                                                                                     |                                                                                                                                                  
aaGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGc                                                                                                                                                    >  4:277114/1‑149 (MQ=255)
       cttTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATc                                                                                                                                               <  3:277114/147‑1 (MQ=255)
        tttATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTAGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATAGCGACGCAAGGCCGTTACCACAAAGTA‑AGTCGTACGCCGctcctc                                                                                                                                            >  1:120340/1‑149 (MQ=255)
          tATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCct                                                                                                                                             <  5:77534/146‑1 (MQ=255)
          tATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCct                                                                                                                                             <  5:349240/146‑1 (MQ=255)
            tCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgt                                                                                                                                         <  5:390469/148‑1 (MQ=255)
                gcgtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTggg                                                                                                                                      <  3:235963/147‑1 (MQ=255)
                  gtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTTGCAGCCC‑ATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACgg                                                                                                                                 <  3:266377/149‑1 (MQ=17)
                     gcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCCATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGc                                                                                                                                <  1:402169/148‑1 (MQ=21)
                     gcgtgAGGCCGGATACGGCGCCGGGGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGc                                                                                                                                <  8:193123/148‑1 (MQ=21)
                          aGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTTGCAGCCC‑ATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGCCTTGCGt                                                                                                                         <  6:236394/149‑1 (MQ=17)
                           ggCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGCTCCTCTGTGGGAGCGGCCTTGCGt                                                                                                                         >  1:307746/1‑149 (MQ=255)
                            gCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTc                                                                                                                        <  5:303268/149‑1 (MQ=255)
                                gATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGa                                                                                                                    >  1:126993/1‑149 (MQ=255)
                                 aTACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTcgc                                                                                                                      >  1:109235/1‑146 (MQ=255)
                                 aTACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTcgc                                                                                                                      <  2:109235/146‑1 (MQ=255)
                                    cGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGACGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATggg                                                                                                                <  5:32518/149‑1 (MQ=255)
                                         ccGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTTTGGGAGCGGCCTTGCGTCGCGATGGGCTGCa                                                                                                           <  1:41178/149‑1 (MQ=255)
                                          cGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCtt                                                                                                                             <  7:203202/130‑1 (MQ=255)
                                            gtgtTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAg                                                                                                        <  6:179470/149‑1 (MQ=255)
                                            gtgtTGGCGGAACTGCGCCGGCAACTCGCGCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTAGCGCGACGCACGGCCGTTCCCACAACGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAg                                                                                                        <  8:192639/149‑1 (MQ=39)
                                               ttGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAg                                                                                                        >  8:183549/1‑146 (MQ=255)
                                                 ggCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGc                                                                                                                           <  7:295268/125‑1 (MQ=255)
                                                 ggCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGc                                                                                                                           >  8:295268/1‑125 (MQ=255)
                                                       aCTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGCTCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGaa                                                                                            >  1:378229/1‑149 (MQ=255)
                                                          gcgcCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATcc                                                                                         >  5:165375/1‑149 (MQ=255)
                                                              cGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAAc                                                                                     <  6:165375/149‑1 (MQ=255)
                                                                  aaCGCGCGCGGGGCGGCGCGGGCCGGGGCCGCTTGGCGGCCCTATCGCGCCCCAAGGCCGTTCCCACAACCAA‑CGGCGTACGCCGATCCGCTGGGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCa                                                                                 <  1:165237/149‑1 (MQ=37)
                                                                   aCTCGCTCGGGGCTGAGGTGGCCTGGGCCGGTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAATGTA‑TGTCGTACGCCTATCCTCTGTGGTTGGGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATGCCAACCTCAt                                                                                <  6:167101/149‑1 (MQ=255)
                                                                    ctcgcgcgGGGCTGAGGTGGCGGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCTTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGTAGCGGCCTTGGGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCAtt                                                                               <  2:120340/149‑1 (MQ=39)
                                                                     tcgctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTc                                                                              >  8:316235/1‑149 (MQ=255)
                                                                     tcgctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTc                                                                              >  3:258954/1‑149 (MQ=255)
                                                                       gctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTc                                                                                  >  4:65279/1‑143 (MQ=255)
                                                                       gctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTc                                                                                  <  3:65279/143‑1 (MQ=255)
                                                                       gctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTc                                                                              <  7:183549/147‑1 (MQ=255)
                                                                       gctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCt                                                                            >  5:157605/1‑149 (MQ=255)
                                                                       gctcgGGGCTGAGCTGGCCGGGGCCGCTTGACGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑AGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTcc                                                                             >  1:242453/1‑148 (MQ=255)
                                                                        ctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCt                                                                            >  5:371416/1‑148 (MQ=255)
                                                                        ctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCCTTCCCACAAAATA‑CGTCGTACGCCGATACTCTGTGGGAGCGGCCGTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATACCAACCAAATTcca                                                                            >  6:231650/1‑147 (MQ=38)
                                                                          cgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGTGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCctct                                                                                                                                           >  4:49450/1‑84 (MQ=255)
                                                                          cgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGTGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCctct                                                                                                                                           <  3:49450/84‑1 (MQ=255)
                                                                           ggggCTGAGCTGGCCGGGGCCGCTTGGGGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCaaa                                                                        <  5:231650/149‑1 (MQ=255)
                                                                           ggggCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCCAGACCCAGAATCCCAACCTCATTCCTCaa                                                                         <  1:237112/149‑1 (MQ=255)
                                                                                       gCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGca                                                            >  3:358261/1‑149 (MQ=255)
                                                                                                     gcggcCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCt                                               <  2:378229/148‑1 (MQ=255)
                                                                                                         cccTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAg                                          <  6:371416/149‑1 (MQ=255)
                                                                                                              tCGCGACGCAAGCCCGTTCCCACAAAGTC‑CGTCGAACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCt                                     <  5:268219/149‑1 (MQ=255)
                                                                                                                  gACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCaa                                 >  3:377223/1‑149 (MQ=255)
                                                                                                                   aCGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAAc                                <  4:358261/149‑1 (MQ=255)
                                                                                                                         ggCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCg                          <  4:377223/149‑1 (MQ=255)
                                                                                                                          gCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCACCCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGa                         >  3:264793/1‑149 (MQ=21)
                                                                                                                           ccGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGGAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGAt                        <  4:258954/149‑1 (MQ=255)
                                                                                                                            cgctCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGAt                        >  5:22914/4‑148 (MQ=21)
                                                                                                                                 ccACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCt                   <  6:157605/148‑1 (MQ=255)
                                                                                                                                  cacaAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCAccc                                                 <  5:145549/117‑1 (MQ=255)
                                                                                                                                  cacaAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCAccc                                                 >  6:145549/1‑117 (MQ=255)
                                                                                                                                         gtccgccgtACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTg           <  2:307746/143‑1 (MQ=255)
                                                                                                                                               cgtACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCgg     >  8:315312/1‑149 (MQ=255)
                                                                                                                                                gtACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGt    <  1:22713/149‑1 (MQ=255)
                                                                                                                                                 tACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTg   >  4:38895/1‑149 (MQ=255)
                                                                                                                                                    gCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGc  >  5:44881/1‑147 (MQ=255)
                                                                                                                                                    gCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGc  >  1:163126/1‑147 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAG‑CAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGC  >  NC_002947/84940‑85233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑