Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	733,647	A→T	10.5%	intergenic (+21/‑26)	PP_t09 → / → PP_t10	tRNA‑Lys/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	733,647	0	A	T	10.5%	173.8 / 8.7	64	intergenic (+21/‑26)	PP_t09/PP_t10	tRNA‑Lys/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base A (29/28);  new base T (4/3);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAC  >  NC_002947/733505‑733793                                                                                                                                               |                                                                                                                                                    gAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATg                                                                                                                                               <  3:138218/149‑1 (MQ=255)   gCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGcc                                                                                                                                             >  7:371921/1‑149 (MQ=255)        aTGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGCCCtttt                                                                                                                                        <  5:339778/149‑1 (MQ=255)           tCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCttttttt                                                                                                                                     <  6:241312/149‑1 (MQ=255)             aGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCtttttttt                                                                                                                                    <  5:228087/148‑1 (MQ=255)             aGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCtttttttt                                                                                                                                    >  6:228087/1‑148 (MQ=255)             aGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCtttttttt                                                                                                                                    <  3:161516/148‑1 (MQ=255)             aGGAGCTCGAATCCCCTCACCCCGCCCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGCGCATCTCAAAGAGATGCCCTTTTTTTTg                                                                                                                                   <  3:313220/149‑1 (MQ=255)              ggAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGa                                                                                                                                  <  7:223933/149‑1 (MQ=255)                     gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATTccc                                                                                                                                            >  5:174440/1‑132 (MQ=255)                     gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGccc                                                                                                                                            <  6:174440/132‑1 (MQ=255)                     gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTc                                                                                                                           >  8:33557/1‑149 (MQ=255)                     gAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTc                                                                                                                           >  3:377583/1‑149 (MQ=255)                      aaTCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGc                                                                                                                                              >  4:398142/1‑129 (MQ=255)                      aaTCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGc                                                                                                                                              <  3:398142/129‑1 (MQ=255)                      aaTCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCg                                                                                                                          >  8:301997/1‑149 (MQ=255)                        tCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCggg                                                                                                                        <  6:296696/149‑1 (MQ=255)                        tCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAACCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCggg                                                                                                                        <  5:266027/149‑1 (MQ=255)                          cccTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGgt                                                                                                                      <  3:253581/149‑1 (MQ=255)                           ccTCACCCCGACCATTTTCCGGGTCGTTAGCTCACTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGgtg                                                                                                                     >  2:40999/1‑149 (MQ=255)                                   cGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCggg                                                                                                                        <  1:217294/138‑1 (MQ=255)                                   cGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCggg                                                                                                                        >  2:217294/1‑138 (MQ=255)                                    gACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGt                                                                                                            >  1:781/1‑149 (MQ=255)                                         ttttCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGt                                                                                                       <  7:33557/149‑1 (MQ=255)                                                         cTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                       <  4:377583/149‑1 (MQ=255)                                                          tCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                       >  4:192692/1‑148 (MQ=255)                                                            aGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCATACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                       <  8:371921/146‑1 (MQ=255)                                                             gTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCATTCCGGTAGCGCGCATGCTTTGG‑GAg                                                                                   >  5:410416/1‑148 (MQ=255)                                                              ttGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTCGGTCCGAATCCTACACGACCCTCATTGTATAATAGGTCTTCTCAAAGAGATGCCATTTTTTTTGATACACTCGTTTTGTAGCGCTGGGCGGTAGCGCGCCTGCTTTGG‑GAGCa                                                                                 <  2:352406/149‑1 (MQ=21)                                                              ttGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCa                                                                                 <  1:40999/149‑1 (MQ=255)                                                                ggTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTg                                                                                        <  6:244586/141‑1 (MQ=255)                                                                ggTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTg                                                                                        >  5:244586/1‑141 (MQ=255)                                                                     agCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGt                                                                           >  1:154083/1‑148 (MQ=255)                                                                       cAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAg                                                                        <  7:301997/149‑1 (MQ=255)                                                                         gTTTGCTTTTAACCAATTGGTCGTTTGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGa                                                                      <  6:410416/149‑1 (MQ=255)                                                                               ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCg                                                                 >  7:319028/1‑148 (MQ=255)                                                                                    aCCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATccc                                                           <  2:154083/149‑1 (MQ=255)                                                                                     ccAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATccc                                                           >  7:387152/1‑148 (MQ=255)                                                                                      cAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCt                                                         <  3:192692/149‑1 (MQ=255)                                                                                      cAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGCAGTTCGAATACCCt                                                         >  2:293502/1‑149 (MQ=255)                                                                                       aaTTGGTAGTAGGTTCGAATCCTACACGACACACAATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCCCAGTCCGGTAGCGAGCCTGCTTTGG‑GAGCAGGATGTCAGGATTTCGAATCCCCt                                                         >  6:19957/1‑148 (MQ=37)                                                                                                    ttCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAttt                                           >  4:271129/1‑149 (MQ=255)                                                                                                        aaTCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTgg                                       <  8:319028/149‑1 (MQ=255)                                                                                                         aTCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGCGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTgg                                       <  1:293502/149‑1 (MQ=255)                                                                                                             tACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGt                                  >  3:304139/1‑149 (MQ=255)                                                                                                               cacGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTAGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTAAGGAGTTCGAATCCCCTCACCACGACCATTTTTGGGTCGt                                  >  8:122310/1‑147 (MQ=255)                                                                                                                    cccACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCt                             >  3:291621/1‑147 (MQ=255)                                                                                                                       accaTGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGtt                        >  7:312796/1‑149 (MQ=255)                                                                                                                         caTGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTgg                      <  4:304139/149‑1 (MQ=255)                                                                                                                          aTGTATAAAAGTTCATCTCAAAGAGATGCCCTTTTTTTTTAAACACTCTGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GATCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGt                     <  5:19957/149‑1 (MQ=18)                                                                                                                             aaaaaaaGGGCATCTCTTTGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAGTTTTGGGTGGTTAGCTGAGTTGGGagg                  >  4:294128/4‑148 (MQ=18)                                                                                                                              aGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGCCCATTTTTGGGTCGTTAGCTCAGTTGGTagag                 >  4:291157/1‑149 (MQ=255)                                                                                                                              aGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTagag                 >  6:338635/1‑149 (MQ=255)                                                                                                                              aGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTaga                  <  8:387152/148‑1 (MQ=255)                                                                                                                                aaaaGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTgg                                       >  6:343406/1‑125 (MQ=255)                                                                                                                                aaaaGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTgg                                       <  5:343406/125‑1 (MQ=255)                                                                                                                                  aaGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCa                           >  7:169420/1‑135 (MQ=255)                                                                                                                                  aaGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCa                           <  8:169420/135‑1 (MQ=255)                                                                                                                                    gggCATCTCTTTGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg           >  6:158846/1‑149 (MQ=255)                                                                                                                                     ggCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTgg          >  5:290461/1‑149 (MQ=255)                                                                                                                                      gCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGc         >  7:75913/1‑149 (MQ=255)                                                                                                                                           tcAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGt             <  6:159021/140‑1 (MQ=255)                                                                                                                                           tcAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGt             >  5:159021/1‑140 (MQ=255)                                                                                                                                           tcAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCtttt     >  5:262519/1‑148 (MQ=255)                                                                                                                                             aaaGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTaa   <  5:338635/148‑1 (MQ=255)                                                                                                                                             aaaGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTaa   >  3:197132/1‑148 (MQ=255)                                                                                                                                             aaaGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAc  <  3:271129/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                    GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCCTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGG‑GAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAC  >  NC_002947/733505‑733793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑