| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 743,266 | G→A | 33.3% | R456Q (CGG→CAG) | PP_0635 → | group II intron‑encoding maturase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 743,266 | 0 | G | A | 33.3% | 9.7 / 4.2 | 12 | R456Q (CGG→CAG) | PP_0635 | group II intron‑encoding maturase |
| Reads supporting (aligned to +/- strand): ref base G (3/5); new base A (2/2); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 | |||||||||||
AAAGAAACTGTGGGACCGGCTCGGGCTGGTCTCGATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTT > NC_002947/743249‑743414 | aaaGAAACTGTGGGACCGGCTCGGGCTGGTCTCGATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTgcgc > 1:236181/1‑149 (MQ=31)aaaGAAACTGTGGGACCGGCTCGGGCTGGTCTCGATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTgcgc > 2:302648/1‑149 (MQ=31)aaaGAAACTGTGGGACCGGCTCGGGCTGGTCTCGATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTgcgc > 2:315678/1‑149 (MQ=31)aaaGAAACTGTGGGACCGGCTCGGGCTGGTCTCGATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTgcgc < 7:41545/149‑1 (MQ=31)aaaGAAACTGTGCGACAGGCGCTGGCTGTTCTCGATACTGGATACGATTAAGCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGTATACGTACGCACTGTGGTGTGAGAGGACGGCGGGTTTGAATCCGCCTCCTACTCGATTgcgc < 8:320288/149‑1 (MQ=11) gAAACTGGGGGACCGGCTCGGGCTGGTCTCGATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGaa < 6:256459/149‑1 (MQ=18) tatcgagACCAGCCCGAGCCGGTCTCGATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCa < 5:267091/143‑1 (MQ=11) tcgagACCAGCCCGAGCCGGTCTCGATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCa < 5:261163/143‑1 (MQ=11) tcgagACCAGCCCGAGCCGGTCTCGATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCa > 6:261163/5‑147 (MQ=11) tcgagACCAGCCCGAGCCGGTCTCGATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCa > 6:267091/5‑147 (MQ=11) tgGGACCGGCTCGGGCTGGTCTCGATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAg < 1:302648/147‑1 (MQ=38) tgGGACCGGCTCGGGCTGGTCTCGATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAg < 7:34197/147‑1 (MQ=38) ggCTCGGGCTGGTCTCGATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCtt > 7:291883/1‑149 (MQ=255) | AAAGAAACTGTGGGACCGGCTCGGGCTGGTCTCGATACTGGATACGATTAACCGGCTTAGCCGCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTT > NC_002947/743249‑743414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |