Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 802,772 T→G 8.0% intergenic (+30/‑56) obg → / → proB GTPase Obg/glutamate 5‑kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947802,7720TG 8.0% 262.3 / 13.1 88intergenic (+30/‑56)obg/proBGTPase Obg/glutamate 5‑kinase
Reads supporting (aligned to +/- strand):  ref base T (40/40);  new base G (3/4);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

CCCGTACCCTGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTCGACCGCGGTG  >  NC_002947/802624‑802918
                                                                                                                                                    |                                                                                                                                                  
tttgTACTCTGCGTTGTACCGGCGTCAAGAGCGTGCATGTCATCGGCGAAGACGATGGTTGGGACTATGATTTCTAGGACTACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGctctct                                                                                                                                                    <  8:177661/146‑1 (MQ=255)
  cGTACCCTGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTgg                                                                                                                                                  >  3:226864/1‑149 (MQ=255)
    tACCCTGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAg                                                                                                                                                <  7:119128/149‑1 (MQ=255)
       ccTGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAgcgg                                                                                                                                             >  3:386645/1‑149 (MQ=255)
            gtcgtACCGGCGTCAAGAGCGTGCATGACATCGGCTACGAAGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGttt                                                                                                                                        <  4:226864/149‑1 (MQ=255)
            gtcgtACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGttt                                                                                                                                        >  2:320814/1‑149 (MQ=255)
             gactACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCCAGAGAGCGGCGttta                                                                                                                                       <  8:294417/146‑2 (MQ=255)
               gtACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGttttt                                                                                                                                      <  4:386645/148‑1 (MQ=255)
               gtACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGttttt                                                                                                                                      <  4:142376/148‑1 (MQ=255)
               gtACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCCAGAGAGCGGCGtttagt                                                                                                                                     <  1:262771/149‑4 (MQ=255)
                tACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTg                                                                                                                                     >  6:419621/1‑148 (MQ=255)
                 aCCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAgcggc                                                                                                                                            >  5:6717/1‑140 (MQ=255)
                 aCCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAgcggc                                                                                                                                            <  6:6717/140‑1 (MQ=255)
                  ccGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTa                                                                                                                                   <  7:271400/148‑1 (MQ=255)
                  ccGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTAt                                                                                                                                  >  1:64469/1‑149 (MQ=255)
                     gCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACCATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCa                                                                                                                               <  5:880/149‑1 (MQ=255)
                      cGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCAc                                                                                                                              >  8:228876/1‑149 (MQ=255)
                       gTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGATGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACg                                                                                                                             >  1:146626/1‑149 (MQ=255)
                       gTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACg                                                                                                                             >  1:294334/1‑149 (MQ=255)
                         cAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACgg                                                                                                                            >  6:389969/1‑148 (MQ=255)
                         cAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACgg                                                                                                                            >  6:395704/1‑148 (MQ=255)
                          aaGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTa                                                                                                                          >  1:354942/1‑149 (MQ=255)
                          aaGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTa                                                                                                                          >  4:291963/1‑149 (MQ=255)
                            gagCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCCAGAGAGCGGCGttta                                                                                                                                       >  6:103343/1‑133 (MQ=255)
                            gagCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCCAGAGAGCGGCGttta                                                                                                                                       <  5:103343/134‑2 (MQ=255)
                               cGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTAc                                                                                                                     <  5:419621/149‑1 (MQ=255)
                                   cATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGAt                                                                                                                 <  4:245127/149‑1 (MQ=255)
                                      gACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTc                                                                                                               >  3:402151/1‑148 (MQ=255)
                                      gACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTc                                                                                                               >  4:276655/1‑148 (MQ=255)
                                       aCATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCg                                                                                                              <  2:294334/148‑1 (MQ=255)
                                            ggCGACGAAGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATa                                                                                                         <  3:316795/148‑1 (MQ=255)
                                             gCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGATTTTGTATCCACGGTATCTACACATTCGACATacg                                                                                                       >  3:32874/1‑147 (MQ=255)
                                                 cgacgaTGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTgg                                                                                                   <  7:228876/149‑1 (MQ=255)
                                                  gacgaTGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGAGATAGGTTgg                                                                                                   >  6:27786/1‑148 (MQ=255)
                                                       tGGTTGGGACGATGATATGCAGGACGACGACGACGCGCCGGAAATCATTAACGTGCGCGACTGATCGGTTGCAGCACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGaagaag                                                                                             <  8:362922/149‑1 (MQ=255)
                                                        ggTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGaagaag                                                                                             <  8:69907/148‑1 (MQ=255)
                                                         gTTGGTACGATGATTTCGAGGAAGACGAAGACGTTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCTACATAGGTTGGAagaaga                                                                                            <  1:150681/148‑1 (MQ=255)
                                                               aCGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAg                                                                                     >  4:194801/1‑149 (MQ=255)
                                                               aCGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAg                                                                                     <  2:164989/149‑1 (MQ=255)
                                                                   tgatTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAg                                                                                 <  7:43049/149‑1 (MQ=255)
                                                                   ctggctCGAGGACGACGAAGACGGTCAGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAg                                                                                 <  8:350737/144‑1 (MQ=255)
                                                                            ggACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGgc                                                                        <  3:276655/149‑1 (MQ=255)
                                                                             gacgacGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGgcg                                                                       <  3:194801/149‑1 (MQ=255)
                                                                             gacgacGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGgcg                                                                       >  2:183026/1‑149 (MQ=255)
                                                                               cgacgaAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATa                                                                                                         <  4:375755/113‑1 (MQ=255)
                                                                               cgacgaAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATa                                                                                                         >  3:375755/1‑113 (MQ=255)
                                                                                 acgaAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGACGAAGATGCGAAGCAAGGTGACGGGCGCCaa                                                                   <  2:191513/149‑1 (MQ=255)
                                                                                 acgaAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGcc                                                                     >  3:354199/1‑147 (MQ=255)
                                                                                      gACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCt                                                              <  4:78169/149‑1 (MQ=255)
                                                                                       aCGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTTTCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTg                                                             <  1:320814/149‑1 (MQ=255)
                                                                                           tCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACgg                                                                          >  7:17643/1‑132 (MQ=255)
                                                                                           tCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACgg                                                                          <  8:17643/132‑1 (MQ=255)
                                                                                             cGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGCTGCGAAGCAAGGTGACCGGCGCCAAGCTCTGGGTCGt                                                       >  5:89147/1‑149 (MQ=255)
                                                                                              ggAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGt                                                       >  4:83569/1‑148 (MQ=255)
                                                                                               gAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATcc                                                                                                                                <  6:215878/74‑1 (MQ=255)
                                                                                               gAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATcc                                                                                                                                >  5:215878/1‑74 (MQ=255)
                                                                                               gAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTa                                                     <  8:374408/149‑1 (MQ=255)
                                                                                                  aTCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGtt                                                      >  2:77784/1‑145 (MQ=255)
                                                                                                  aTCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGa                                                  <  2:146626/149‑1 (MQ=255)
                                                                                                  aTCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGa                                                  <  3:184351/149‑1 (MQ=255)
                                                                                                   tCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGAt                                                 >  6:132273/1‑149 (MQ=255)
                                                                                                   tCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGAt                                                 <  4:354199/149‑1 (MQ=255)
                                                                                                     aTTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACTGATTCGACATAGGTTGGAAGAAGATGCGAAGCTAGGTGACGGGCGCCAAGCGCTGGGTCGTGAAGAttt                                               >  8:357254/1‑148 (MQ=255)
                                                                                                     aTTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGAtt                                                <  2:64469/148‑1 (MQ=255)
                                                                                                     aTTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGAtt                                                <  3:291963/148‑1 (MQ=255)
                                                                                                     aTTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGAtt                                                <  6:390704/148‑1 (MQ=255)
                                                                                                                cgACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTctg                                    <  7:332191/149‑1 (MQ=255)
                                                                                                                cgACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTctg                                    >  3:48688/1‑149 (MQ=255)
                                                                                                                 gACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGct                                       >  5:75918/1‑145 (MQ=255)
                                                                                                                 gACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGct                                       <  6:75918/145‑1 (MQ=255)
                                                                                                                   cTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTctgct                                  >  8:13696/1‑148 (MQ=255)
                                                                                                                             gCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGAt                        <  1:183026/148‑1 (MQ=255)
                                                                                                                              cAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACTGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATg                       <  5:389969/148‑1 (MQ=255)
                                                                                                                              cAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATg                       <  5:395704/148‑1 (MQ=255)
                                                                                                                              cAGTACACTAAACGCCGCTCTCAGGAGCGGCCGTTTTTTATCCAACGTATCTACAGAATCGACATAGGTTGGAAGAAGTAGCGAAGCAATGTGACGGGCGCAAACCGCTGGGTAGTTAAGATTTGCAGAGCTCTTCTGACCGCCGATgg                      >  6:232094/1‑149 (MQ=255)
                                                                                                                                 tACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGc                     >  4:347223/1‑147 (MQ=255)
                                                                                                                                  acacTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAg                  >  8:190110/1‑149 (MQ=255)
                                                                                                                                   cacTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACTGTATCTACAGATTAGAAATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAgg                 >  2:334399/1‑149 (MQ=255)
                                                                                                                                    acTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACgg                                                                          >  2:232440/1‑91 (MQ=255)
                                                                                                                                    acTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACgg                                                                          <  1:232440/91‑1 (MQ=255)
                                                                                                                                    acTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAggg                >  5:103204/1‑149 (MQ=255)
                                                                                                                                     aaaaaCGCCGCTCCAGAGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGc               >  2:392293/3‑149 (MQ=255)
                                                                                                                                        aaCGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTc            <  5:132273/149‑1 (MQ=255)
                                                                                                                                         aCGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCCTTAAGATTGGCAGTGCTCTGCTGACCGCCGAAGGCAAGGCCCTc            >  1:341325/1‑148 (MQ=255)
                                                                                                                                         aCGCCGCTCCAGAGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCaa                                                                   <  3:35681/93‑1 (MQ=39)
                                                                                                                                         aCGCCGCTCCAGAGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCaa                                                                   >  4:35681/1‑93 (MQ=39)
                                                                                                                                           gccgcTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAGGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTCGAc         >  5:279530/1‑149 (MQ=255)
                                                                                                                                           gccgcTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTCGAc         >  2:407859/1‑149 (MQ=255)
                                                                                                                                             cgcTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTCGACcg       >  7:109205/1‑149 (MQ=255)
                                                                                                                                                    tGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTCGACCGCGGTg  <  1:407859/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCCGTACCCTGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTCGACCGCGGTG  >  NC_002947/802624‑802918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑