Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	809,094	A→C	5.4%	R88R (CGT→CGG)	PP_0695 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	809,094	0	A	C	5.4%	270.1 / 4.4	88	R88R (CGT→CGG)	PP_0695	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (34/49);  new base C (3/2);  total (37/51)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

GCGGTTGTCGAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGACCAGGCTCAGGGTGCCCTGGTCGAAA  >  NC_002947/808953‑809241                                                                                                                                                |                                                                                                                                                       gcgGTTGTCGAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTc                                                                                                                                                  <  4:292123/148‑1 (MQ=255) gcgGTTGTCGAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTc                                                                                                                                                  <  5:111694/148‑1 (MQ=255) gcgGTTGTCGAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTc                                                                                                                                                  <  6:246531/148‑1 (MQ=255) gcgGTTGTCGAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCCCGAACCGTTTc                                                                                                                                                  >  1:13784/1‑148 (MQ=255)  cgGTTGTCGAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCgg                                                                                                                                                >  6:335134/1‑149 (MQ=255)  cgGTTGTCGAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCgg                                                                                                                                                >  7:293902/1‑149 (MQ=255)   ggTTGTCGAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCggg                                                                                                                                               <  7:87779/149‑1 (MQ=255)     ttGTCGAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGct                                                                                                                                             <  3:382254/149‑1 (MQ=255)        tCGAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGctct                                                                                                                                           <  7:85406/148‑1 (MQ=255)         cGAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTg                                                                                                                                         >  8:95235/1‑149 (MQ=255)          gAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTgg                                                                                                                                        >  6:404851/1‑149 (MQ=255)          gAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTgg                                                                                                                                        >  5:193996/1‑149 (MQ=255)          gAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCCCGAACCGTTTCGAGctcaac                                                                                                                                         <  2:13784/148‑4 (MQ=255)          gAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTACGCTTCGA‑TCTCCAGGCACAGGCCCACCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGGGGATCTGACGCGTTGAGCCCGAACCGTTTCGAGctcaac                                                                                                                                         <  1:41811/149‑4 (MQ=255)           aaGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTAGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGc                                                                                                                                       <  4:203431/149‑1 (MQ=255)             ggCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCa                                                                                                                                     >  8:237165/1‑149 (MQ=255)               cATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCCCTTGGCCAcc                                                                                                                                   >  8:197548/1‑149 (MQ=255)                aTCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCg                                                                                                                                  >  3:249709/1‑149 (MQ=255)                 tCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGGTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCgg                                                                                                                                 <  6:335075/149‑1 (MQ=255)                    tGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGAc                                                                                                                              <  1:347493/149‑1 (MQ=255)                     ggATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGAGTCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGAc                                                                                                                              >  1:288133/1‑149 (MQ=255)                      gATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑a                                                                                                                           <  5:176225/149‑1 (MQ=255)                      gATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑a                                                                                                                           <  8:293902/149‑1 (MQ=255)                          tgCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑Gat                                                                                                                      <  7:140829/149‑1 (MQ=255)                          tgCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTGCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑Gat                                                                                                                      <  5:297516/149‑1 (MQ=255)                           gCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGC‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAACCGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑Gata                                                                                                                     >  7:357311/1‑149 (MQ=255)                           gCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑Gata                                                                                                                     <  3:5661/149‑1 (MQ=255)                            cccGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑Gatat                                                                                                                    >  1:163013/1‑149 (MQ=255)                              cGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATcc                                                                                                                  <  2:288133/149‑1 (MQ=255)                                gCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAg                                                                                                                >  2:255365/1‑149 (MQ=255)                                  cAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGc                                                                                                              <  2:42703/149‑1 (MQ=255)                                      ggcTCCTGGTGGCGCATGTTGGCTTCTT‑TCTCCTGCCACTGCCCCCGCCGGTGTTCGCGGCGCTGTTAGGCTTGCAAGTCGGGTTTCTG‑CGGGTTGTGCTCTAAACGGTTCGGGCTCTTGGCCACCGGGACC‑TG‑TATATCCCGGCgtac                                                                                                          <  6:193996/148‑4 (MQ=255)                                                 gcgcAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGGTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGa                                                                                              <  4:258527/149‑1 (MQ=255)                                                    cAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTa                                                                                           <  4:249709/149‑1 (MQ=255)                                                       gTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCt                                                                                        <  7:317389/149‑1 (MQ=255)                                                          ggCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGATGAAGTCGTGGATCTG‑CGCGTTGAGATCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AGTGATATCCAGGCGGATCCACA‑CGTCGAGTAGCTgg                                                                                      <  1:55140/149‑1 (MQ=255)                                                          ggCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTgg                                                                                      <  5:335134/148‑1 (MQ=255)                                                          ggCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGG‑AGTCTAGGATCTG‑CGCGTAGAGCTCGAAACCGTTCGCGCTCTTGCCCACCGCGACC‑AC‑GATATCCAGGCGGATCCACA‑CGTCGAGTAACTGCGt                                                                                    >  8:274956/1‑149 (MQ=255)                                                            cTTCTA‑TCTCCAGGCACAGGCGCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCTTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGt                                                                                    <  3:185429/148‑1 (MQ=255)                                                             ttCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCa                                                                                                                 >  8:323162/1‑119 (MQ=255)                                                             ttCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCa                                                                                                                 <  7:323162/119‑1 (MQ=255)                                                             ttCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGt                                                                                    <  6:216920/147‑1 (MQ=255)                                                             ttCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGt                                                                                    >  5:216920/1‑147 (MQ=255)                                                             ttCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGa                                                                                  >  1:210680/1‑149 (MQ=255)                                                               cGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGG‑AGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATg                                                                                <  7:274956/148‑1 (MQ=255)                                                                    ctcCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCt                                                                            >  5:93676/1‑149 (MQ=255)                                                                       cAGGCACAGTCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCTAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTcc                                                                             <  4:327613/145‑1 (MQ=255)                                                                       cAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGc                                                                          <  7:197548/148‑1 (MQ=255)                                                                        aGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGATACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCgg                                                                        <  5:117119/149‑1 (MQ=255)                                                                        aGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCgg                                                                        >  6:205765/1‑149 (MQ=255)                                                                          gCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTgg                                                                                      >  1:257294/1‑133 (MQ=255)                                                                          gCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTgg                                                                                      <  2:257294/133‑1 (MQ=255)                                                                          gCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCgggg                                                                      >  4:206853/1‑149 (MQ=255)                                                                          gCACAGGCCAAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTCGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGCGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTACCTGTGTGATGACCAGCgggc                                                                      >  6:255835/1‑148 (MQ=255)                                                                            ccaGGCCCAGCCGGGGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGTATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTg                                                                    <  2:304578/148‑1 (MQ=255)                                                                                gCCCAGCCGGTGCTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCttt                                                                <  7:222768/149‑1 (MQ=255)                                                                                  ccAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGATGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTc                                                               >  1:96926/1‑148 (MQ=255)                                                                                   cAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTGCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGa                                                             <  7:237165/149‑1 (MQ=255)                                                                                     gCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCTGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAgc                                                           <  2:269172/149‑1 (MQ=255)                                                                                      ccGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAgct                                                          >  2:223619/1‑149 (MQ=255)                                                                                        ggTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAgctgc                                                        <  1:255365/149‑1 (MQ=255)                                                                                         gtgtTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAgctgct                                                       >  7:153687/1‑149 (MQ=255)                                                                                            ttCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGc                                                    >  7:27621/1‑149 (MQ=255)                                                                                              cgcgGCGGATGTTGGCTAGTAAGTCGTGGATCTG‑CGGGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCTGATCCACA‑CGTCGAGTAGCTGGGTGATGTGCTGCGGGGTGCTTTCGAGCTGCTGGCg                                                   <  5:255835/148‑1 (MQ=255)                                                                                               gcggagCAGGTAGGCGAGGAATTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGGTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCCGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCgg                                                  <  8:357311/148‑1 (MQ=255)                                                                                                 ggcgCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGcc                                               <  6:106129/149‑1 (MQ=255)                                                                                                          aGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGt                                                                     <  6:246205/118‑1 (MQ=255)                                                                                                             cGAGGAAGTCGTGGATCTG‑CGCGGTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGTGACC‑AG‑GATTTCCAGGCGGATCCACT‑CGTCGTGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGgcg                                    <  3:247118/148‑1 (MQ=255)                                                                                                              gAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACCAAG‑GATATCAACGCGCATCAACA‑CGTCGAGTAGATGGGTGATTTCCTGCGGGGTGCTTTCGAGCTGATGGCGGGCAAAATGATGgcc                                    >  1:319393/1‑147 (MQ=255)                                                                                                              gAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGgcg                                    >  2:403062/1‑147 (MQ=255)                                                                                                                    gTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTCGCTGGGTGATGTCCTGCGGGGTGCTTTCGACCTGCTGGCGGGCCACCTGCTGGCGCAGTTcta                            >  7:134889/1‑147 (MQ=255)                                                                                                                     tCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGAc                           <  3:206853/149‑1 (MQ=255)                                                                                                                       gTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGAc                           >  6:297311/1‑147 (MQ=255)                                                                                                                       gTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGAc                           >  5:185934/1‑147 (MQ=255)                                                                                                                       gTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGACCa                         <  6:185934/149‑1 (MQ=255)                                                                                                                        tGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGACCAg                        <  5:404851/149‑1 (MQ=255)                                                                                                                         ggATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTgg                                      >  3:360160/1‑134 (MQ=255)                                                                                                                         ggATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTgg                                      <  4:360160/134‑1 (MQ=255)                                                                                                                         ggATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGACCAgg                       >  7:123716/1‑149 (MQ=255)                                                                                                                            tCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTc                                                               >  1:7546/1‑106 (MQ=255)                                                                                                                            tCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTc                                                               <  2:7546/106‑1 (MQ=255)                                                                                                                                 cgcgTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGATCTGCTGGCGGGCCACATGCTGGCGCAGTTCGACCAGGCTCAgg                 <  5:205765/148‑1 (MQ=255)                                                                                                                                 cgcgTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGACCAGGCTCAgg                 <  8:123716/148‑1 (MQ=255)                                                                                                                                  gcgTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGACCAGGCTCAGGGt               <  1:223619/149‑1 (MQ=255)                                                                                                                                    caagAGCCCGAACCGTTTCGAGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCAGA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGACCAGGCTCAGGGTGc             >  8:89767/4‑149 (MQ=255)                                                                                                                                         gCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGACCAGGCTCAGGGTGCCCTgg        >  1:338118/1‑149 (MQ=255)                                                                                                                                         gCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGACCAGGCTCAGGGTGCCCTgg        <  2:96926/149‑1 (MQ=255)                                                                                                                                           tCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGACCAGGCTCAGGGTGCCCTGGTc      >  4:159226/1‑149 (MQ=255)                                                                                                                                              aaaCGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGACCAGGCTCAGGGTGCCCTGGTCGaa   <  8:27621/149‑1 (MQ=255)                                                                                                                                               aaCGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGACCAGGCTCAGGGTGCCCTGGTCGaaa  <  1:403062/149‑1 (MQ=255)                                                                                                                                                aCGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACAGCGTCGAGTTGGTGGGTGATGTCATGCGGGGTGGTTTGGAGGTGCTGGCGGGCCACATGGTGGCGCAGTACGACCAGGCTCAGGGTGCCCTGTTCGaac  >  1:99962/1‑148 (MQ=255)                                                                                                                                                |                                                                                                                                                       GCGGTTGTCGAAGGCATCCTGGATGTGCCCGGCCAGGCGCTCCAGGTGGCGCAAGTAGGCTTCGA‑TCTCCAGGCACAGGCCCAGCCGGTGTTCGCGGCGCAGGTAGGCGAGGAAGTCGTGGATCTG‑CGCGTTGAGCTCGAAACGGTTCGGGCTCTTGGCCACCGGGACC‑AG‑GATATCCAGGCGGATCCACA‑CGTCGAGTAGCTGGGTGATGTCCTGCGGGGTGCTTTCGAGCTGCTGGCGGGCCACATGCTGGCGCAGTTCGACCAGGCTCAGGGTGCCCTGGTCGAAA  >  NC_002947/808953‑809241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑