Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	866,725	G→A	6.6%	D60N (GAC→AAC) ‡	PP_0748 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	866,725	0	G	A	6.6%	275.1 / 5.1	90	D60N (GAC→AAC) ‡	PP_0748	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (41/43);  new base A (3/3);  total (44/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGTTCGCCGGGCTGGCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGG  >  NC_002947/866577‑866872                                                                                                                                                     |                                                                                                                                                    gCTGTTCGCCGGGCTGGCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCg                                                                                                                                                     <  1:154914/149‑1 (MQ=255)  cTGTTCGCCGGGCTGGCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGa                                                                                                                                                    <  6:125988/149‑1 (MQ=255)        gCCGGGCTGGCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGc                                                                                                                                              >  5:348625/1‑149 (MQ=255)        gCCGGGCTGGCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGc                                                                                                                                              >  2:231529/1‑149 (MQ=255)                ggCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCt                                                                                                                                      <  7:324675/149‑1 (MQ=255)                  ccGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCtt                                                                                                                                     >  1:18284/1‑148 (MQ=255)                   cGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGa                                                                                                                                   <  6:348625/149‑1 (MQ=255)                    gAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGa                                                                                                                                   >  5:107461/1‑148 (MQ=255)                    gAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGAt                                                                                                                                  <  5:183201/149‑1 (MQ=255)                     aaaaGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGa                                                                                                                                   >  5:81900/1‑147 (MQ=255)                         ggCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAgcgc                                                                                                                             <  2:130701/149‑1 (MQ=255)                            cGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTc                                                                                                                           >  3:91998/1‑148 (MQ=255)                             gCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCaa                                                                                                                         <  5:289752/149‑1 (MQ=255)                                gCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAggg                                                                                                                      <  8:158695/149‑1 (MQ=255)                                gCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAggg                                                                                                                      >  1:404251/1‑149 (MQ=255)                                 ccAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTg                                                                                                                                    >  4:209815/1‑134 (MQ=255)                                 ccAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTg                                                                                                                                    <  3:209815/134‑1 (MQ=255)                                  cAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGc                                                                                                                    >  6:86045/1‑149 (MQ=255)                                  cAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGc                                                                                                                    >  2:77659/1‑149 (MQ=255)                                   aaCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCt                                                                                                                                      <  8:205368/130‑1 (MQ=255)                                   aaCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCg                                                                                                                   >  4:173217/1‑149 (MQ=255)                                   aaCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCCTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCCCTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaatggc                                                                                                                    >  4:384215/1‑143 (MQ=21)                                    aCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTCAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGt                                                                                                                  <  6:81900/149‑1 (MQ=255)                                     cccGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGtt                                                                                                                 >  4:392767/1‑149 (MQ=255)                                      ccGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGttt                                                                                                                >  5:309946/1‑149 (MQ=255)                                       cgcgcgGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCCCTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCgatgc                                                                                                               <  3:384215/149‑5 (MQ=21)                                       cgcgcgGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGtttt                                                                                                               <  5:86045/149‑1 (MQ=255)                                          gcgggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGc                                                                                                            <  6:107461/149‑1 (MQ=255)                                            gggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGGTAGCGCTCAAGGGGCGTTTTGGCCt                                                                                                          >  5:41810/1‑149 (MQ=255)                                            gggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCt                                                                                                          <  4:188714/149‑1 (MQ=255)                                                ggcAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGtt                                                                                                       >  5:49371/1‑148 (MQ=255)                                                ggcAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGtt                                                                                                       >  8:31353/1‑148 (MQ=255)                                                 gcagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGa                                                                                                     <  8:298845/149‑1 (MQ=255)                                                 gcagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCATGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGa                                                                                                     >  1:179466/1‑149 (MQ=255)                                                  cagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGAt                                                                                                    <  4:91998/149‑1 (MQ=255)                                                   agcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATc                                                                                                   >  6:93957/1‑149 (MQ=255)                                                   agcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATc                                                                                                   <  3:392767/149‑1 (MQ=255)                                                      aCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTc                                                                                                >  2:39692/1‑149 (MQ=255)                                                       ccATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGGTAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCg                                                                                               <  6:41810/149‑1 (MQ=255)                                                        cATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGTAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGa                                                                                              <  8:370384/149‑1 (MQ=255)                                                          tAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGACGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATg                                                                                            <  5:351053/149‑1 (MQ=255)                                                              tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCg                                                                                                                   >  8:112238/1‑122 (MQ=21)                                                              tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAAc                                                                                         >  7:320493/1‑148 (MQ=255)                                                              tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGGCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCg                                                                                                                   <  7:112238/122‑1 (MQ=21)                                                                tCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGTGGCGTTTTGGCCTGTTGATCTTCGATGAACAAg                                                                                      >  4:366865/1‑149 (MQ=255)                                                                   cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaa                                                                                                                        <  8:359492/112‑2 (MQ=25)                                                                   cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaa                                                                                                                        >  7:359492/1‑111 (MQ=25)                                                                   cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGgcg                                                                                   <  7:31353/149‑1 (MQ=255)                                                                        tGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCgg                                                                                <  8:320493/147‑1 (MQ=255)                                                                          gAAGAGCCCTGCCACCGCAGGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgac                                                                            <  4:78741/149‑1 (MQ=255)                                                                           aaGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgact                                                                           >  4:387485/1‑149 (MQ=255)                                                                              agCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgactgac                                                                        <  6:68308/149‑1 (MQ=255)                                                                                  cTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGc                                                                    >  6:14479/1‑149 (MQ=255)                                                                                     ccACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCggg                                                                 <  2:179466/149‑1 (MQ=255)                                                                                            aTGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGtgctg                                                            >  7:116263/1‑147 (MQ=255)                                                                                             tGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAg                                                         <  1:131854/149‑1 (MQ=255)                                                                                               gCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGc                                                       <  4:351117/149‑1 (MQ=255)                                                                                                   tCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGCCAAGCGGGTGCTGCAGGCCGGt                                                   >  5:248333/1‑149 (MQ=255)                                                                                                         tGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGAc                                             >  3:100530/1‑149 (MQ=255)                                                                                                         tGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGGGACTGACAAGCGGGTGCTGCAGGACGGTGGCGAc                                             >  5:415148/1‑149 (MQ=255)                                                                                                         tGCAACCAAGCACATACATTCCGCCGGATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGAc                                             <  8:297465/149‑1 (MQ=255)                                                                                                            aaCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGt                                                                               >  1:329074/1‑112 (MQ=255)                                                                                                            aaCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGt                                                                               <  2:329074/112‑1 (MQ=255)                                                                                                             aCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTc                                         >  2:209744/1‑149 (MQ=255)                                                                                                                aaGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTgg                                      >  4:82382/1‑149 (MQ=255)                                                                                                                 aGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTggg                                     <  8:300230/149‑1 (MQ=255)                                                                                                                  gCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                    >  6:227403/1‑149 (MQ=255)                                                                                                                    aCCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgtgt                                  <  6:309946/149‑1 (MQ=255)                                                                                                                    aCCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgtgt                                  >  7:20345/1‑149 (MQ=255)                                                                                                                     ccTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTgg                                                 <  2:290396/133‑1 (MQ=255)                                                                                                                     ccTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGtgtg                                 <  1:77659/149‑1 (MQ=255)                                                                                                                      cTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGtgtg                                 >  8:261867/1‑148 (MQ=255)                                                                                                                       tACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGa                               >  2:351635/1‑149 (MQ=255)                                                                                                                        aCATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGAt                              >  1:295932/1‑149 (MQ=255)                                                                                                                         cATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATc                             >  3:163167/1‑149 (MQ=255)                                                                                                                         cATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCACGCCGGTGGCGACTGTCTGGGTGTGGATc                             <  1:231529/149‑1 (MQ=255)                                                                                                                          aTTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                            <  7:261867/149‑1 (MQ=255)                                                                                                                          aTTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                            <  4:203979/149‑1 (MQ=255)                                                                                                                              cgccgcATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGgc                         <  5:14479/148‑1 (MQ=255)                                                                                                                                        ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                    <  1:239826/127‑1 (MQ=255)                                                                                                                                        ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                    >  2:239826/1‑127 (MQ=255)                                                                                                                                        ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacc               >  7:322733/1‑148 (MQ=255)                                                                                                                                        ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacc               >  8:291703/1‑148 (MQ=255)                                                                                                                                          aTTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacctac            <  8:20345/149‑1 (MQ=255)                                                                                                                                              gAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCAt         <  3:173217/148‑1 (MQ=255)                                                                                                                                               aGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCAta        <  1:209744/148‑2 (MQ=255)                                                                                                                                               aGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATgg       >  3:251323/1‑149 (MQ=255)                                                                                                                                                gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc      <  6:49371/149‑1 (MQ=255)                                                                                                                                                gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc      <  3:387485/149‑1 (MQ=255)                                                                                                                                                gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc      >  2:243257/1‑149 (MQ=255)                                                                                                                                                    cGACAAGGCTGAAACCTTGTTTGCGCTCAAGGGGCGTTTTGGCCTGTTGAGCTTCGATGAACAATGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtgg  <  8:376001/149‑1 (MQ=255)                                                                                                                                                     gACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTg                    >  4:386799/1‑130 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GCTGTTCGCCGGGCTGGCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGG  >  NC_002947/866577‑866872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑