Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	866,726	A→T	6.6%	D60V (GAC→GTC) ‡	PP_0748 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	866,726	0	A	T	6.6%	280.3 / 5.6	90	D60V (GAC→GTC) ‡	PP_0748	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (42/42);  new base T (3/3);  total (45/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CTGTTCGCCGGGCTGGCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTG  >  NC_002947/866578‑866874                                                                                                                                                     |                                                                                                                                                     cTGTTCGCCGGGCTGGCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGa                                                                                                                                                      <  6:125988/149‑1 (MQ=255)       gCCGGGCTGGCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGc                                                                                                                                                >  5:348625/1‑149 (MQ=255)       gCCGGGCTGGCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGc                                                                                                                                                >  2:231529/1‑149 (MQ=255)               ggCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCt                                                                                                                                        <  7:324675/149‑1 (MQ=255)                 ccGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCtt                                                                                                                                       >  1:18284/1‑148 (MQ=255)                  cGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGa                                                                                                                                     <  6:348625/149‑1 (MQ=255)                   gAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGa                                                                                                                                     >  5:107461/1‑148 (MQ=255)                   gAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGAt                                                                                                                                    <  5:183201/149‑1 (MQ=255)                    aaaaGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGa                                                                                                                                     >  5:81900/1‑147 (MQ=255)                        ggCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAgcgc                                                                                                                               <  2:130701/149‑1 (MQ=255)                           cGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTc                                                                                                                             >  3:91998/1‑148 (MQ=255)                            gCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCaa                                                                                                                           <  5:289752/149‑1 (MQ=255)                               gCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAggg                                                                                                                        <  8:158695/149‑1 (MQ=255)                               gCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAggg                                                                                                                        >  1:404251/1‑149 (MQ=255)                                ccAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTg                                                                                                                                      >  4:209815/1‑134 (MQ=255)                                ccAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTg                                                                                                                                      <  3:209815/134‑1 (MQ=255)                                 cAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGc                                                                                                                      >  6:86045/1‑149 (MQ=255)                                 cAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGc                                                                                                                      >  2:77659/1‑149 (MQ=255)                                  aaCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCt                                                                                                                                        <  8:205368/130‑1 (MQ=255)                                  aaCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCg                                                                                                                     >  4:173217/1‑149 (MQ=255)                                  aaCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCCTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCCCTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaatggc                                                                                                                      >  4:384215/1‑143 (MQ=21)                                   aCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTCAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGt                                                                                                                    <  6:81900/149‑1 (MQ=255)                                    cccGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGtt                                                                                                                   >  4:392767/1‑149 (MQ=255)                                     ccGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGttt                                                                                                                  >  5:309946/1‑149 (MQ=255)                                      cgcgcgGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCCCTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCgatgc                                                                                                                 <  3:384215/149‑5 (MQ=21)                                      cgcgcgGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGtttt                                                                                                                 <  5:86045/149‑1 (MQ=255)                                         gcgggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGc                                                                                                              <  6:107461/149‑1 (MQ=255)                                           gggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGGTAGCGCTCAAGGGGCGTTTTGGCCt                                                                                                            >  5:41810/1‑149 (MQ=255)                                           gggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCt                                                                                                            <  4:188714/149‑1 (MQ=255)                                               ggcAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGtt                                                                                                         >  5:49371/1‑148 (MQ=255)                                               ggcAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGtt                                                                                                         >  8:31353/1‑148 (MQ=255)                                                gcagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGa                                                                                                       <  8:298845/149‑1 (MQ=255)                                                gcagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCATGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGa                                                                                                       >  1:179466/1‑149 (MQ=255)                                                 cagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGAt                                                                                                      <  4:91998/149‑1 (MQ=255)                                                  agcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATc                                                                                                     <  3:392767/149‑1 (MQ=255)                                                  agcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATc                                                                                                     >  6:93957/1‑149 (MQ=255)                                                     aCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTc                                                                                                  >  2:39692/1‑149 (MQ=255)                                                      ccATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGGTAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCg                                                                                                 <  6:41810/149‑1 (MQ=255)                                                       cATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGTAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGa                                                                                                <  8:370384/149‑1 (MQ=255)                                                         tAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGACGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATg                                                                                              <  5:351053/149‑1 (MQ=255)                                                             tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCg                                                                                                                     >  8:112238/1‑122 (MQ=21)                                                             tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAAc                                                                                           >  7:320493/1‑148 (MQ=255)                                                             tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGGCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCg                                                                                                                     <  7:112238/122‑1 (MQ=21)                                                               tCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGTGGCGTTTTGGCCTGTTGATCTTCGATGAACAAg                                                                                        >  4:366865/1‑149 (MQ=255)                                                                  cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaa                                                                                                                          >  7:359492/1‑111 (MQ=25)                                                                  cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaa                                                                                                                          <  8:359492/112‑2 (MQ=25)                                                                  cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGgcg                                                                                     <  7:31353/149‑1 (MQ=255)                                                                       tGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCgg                                                                                  <  8:320493/147‑1 (MQ=255)                                                                         gAAGAGCCCTGCCACCGCAGGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgac                                                                              <  4:78741/149‑1 (MQ=255)                                                                          aaGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgact                                                                             >  4:387485/1‑149 (MQ=255)                                                                             agCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgactgac                                                                          <  6:68308/149‑1 (MQ=255)                                                                                 cTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGc                                                                      >  6:14479/1‑149 (MQ=255)                                                                                    ccACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCggg                                                                   <  2:179466/149‑1 (MQ=255)                                                                                           aTGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGtgctg                                                              >  7:116263/1‑147 (MQ=255)                                                                                            tGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAg                                                           <  1:131854/149‑1 (MQ=255)                                                                                              gCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGc                                                         <  4:351117/149‑1 (MQ=255)                                                                                                  tCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGCCAAGCGGGTGCTGCAGGCCGGt                                                     >  5:248333/1‑149 (MQ=255)                                                                                                        tGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGAc                                               >  3:100530/1‑149 (MQ=255)                                                                                                        tGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGGGACTGACAAGCGGGTGCTGCAGGACGGTGGCGAc                                               >  5:415148/1‑149 (MQ=255)                                                                                                        tGCAACCAAGCACATACATTCCGCCGGATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGAc                                               <  8:297465/149‑1 (MQ=255)                                                                                                           aaCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGt                                                                                 >  1:329074/1‑112 (MQ=255)                                                                                                           aaCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGt                                                                                 <  2:329074/112‑1 (MQ=255)                                                                                                            aCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTc                                           >  2:209744/1‑149 (MQ=255)                                                                                                               aaGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTgg                                        >  4:82382/1‑149 (MQ=255)                                                                                                                aGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTggg                                       <  8:300230/149‑1 (MQ=255)                                                                                                                 gCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                      >  6:227403/1‑149 (MQ=255)                                                                                                                   aCCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgtgt                                    <  6:309946/149‑1 (MQ=255)                                                                                                                   aCCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgtgt                                    >  7:20345/1‑149 (MQ=255)                                                                                                                    ccTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTgg                                                   <  2:290396/133‑1 (MQ=255)                                                                                                                    ccTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGtgtg                                   <  1:77659/149‑1 (MQ=255)                                                                                                                     cTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGtgtg                                   >  8:261867/1‑148 (MQ=255)                                                                                                                      tACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGa                                 >  2:351635/1‑149 (MQ=255)                                                                                                                       aCATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGAt                                >  1:295932/1‑149 (MQ=255)                                                                                                                        cATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATc                               >  3:163167/1‑149 (MQ=255)                                                                                                                        cATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCACGCCGGTGGCGACTGTCTGGGTGTGGATc                               <  1:231529/149‑1 (MQ=255)                                                                                                                         aTTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                              <  4:203979/149‑1 (MQ=255)                                                                                                                         aTTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                              <  7:261867/149‑1 (MQ=255)                                                                                                                             cgccgcATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGgc                           <  5:14479/148‑1 (MQ=255)                                                                                                                                       ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                      >  2:239826/1‑127 (MQ=255)                                                                                                                                       ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                      <  1:239826/127‑1 (MQ=255)                                                                                                                                       ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacc                 >  7:322733/1‑148 (MQ=255)                                                                                                                                       ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacc                 >  8:291703/1‑148 (MQ=255)                                                                                                                                         aTTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacctac              <  8:20345/149‑1 (MQ=255)                                                                                                                                             gAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCAt           <  3:173217/148‑1 (MQ=255)                                                                                                                                              aGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCAta          <  1:209744/148‑2 (MQ=255)                                                                                                                                              aGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATgg         >  3:251323/1‑149 (MQ=255)                                                                                                                                               gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc        <  6:49371/149‑1 (MQ=255)                                                                                                                                               gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc        <  3:387485/149‑1 (MQ=255)                                                                                                                                               gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc        >  2:243257/1‑149 (MQ=255)                                                                                                                                                   cGACAAGGCTGAAACCTTGTTTGCGCTCAAGGGGCGTTTTGGCCTGTTGAGCTTCGATGAACAATGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtgg    <  8:376001/149‑1 (MQ=255)                                                                                                                                                    gACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTg                      >  4:386799/1‑130 (MQ=255)                                                                                                                                                     aCAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtggtg  <  3:82382/149‑1 (MQ=255)                                                                                                                                                     aCAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtggtg  <  2:404251/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CTGTTCGCCGGGCTGGCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTG  >  NC_002947/866578‑866874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑