Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	866,735	A→C	5.8%	E63A (GAA→GCA) ‡	PP_0748 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	866,735	0	A	C	5.8%	314.3 / 7.1	99	E63A (GAA→GCA) ‡	PP_0748	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (44/48);  new base C (3/3);  total (47/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

GGCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTGG  >  NC_002947/866592‑866881                                                                                                                                                |                                                                                                                                                   ggCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCt                                                                                                                                               <  7:324675/149‑1 (MQ=255)   ccGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCtt                                                                                                                                              >  1:18284/1‑148 (MQ=255)    cGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGa                                                                                                                                            <  6:348625/149‑1 (MQ=255)     gAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGa                                                                                                                                            >  5:107461/1‑148 (MQ=255)     gAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGAt                                                                                                                                           <  5:183201/149‑1 (MQ=255)      aaaaGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGa                                                                                                                                            >  5:81900/1‑147 (MQ=255)          ggCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAgcgc                                                                                                                                      <  2:130701/149‑1 (MQ=255)             cGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTc                                                                                                                                    >  3:91998/1‑148 (MQ=255)              gCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCaa                                                                                                                                  <  5:289752/149‑1 (MQ=255)                 gCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAggg                                                                                                                               >  1:404251/1‑149 (MQ=255)                 gCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAggg                                                                                                                               <  8:158695/149‑1 (MQ=255)                  ccAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTg                                                                                                                                             <  3:209815/134‑1 (MQ=255)                  ccAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTg                                                                                                                                             >  4:209815/1‑134 (MQ=255)                   cAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGc                                                                                                                             >  6:86045/1‑149 (MQ=255)                   cAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGc                                                                                                                             >  2:77659/1‑149 (MQ=255)                    aaCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCt                                                                                                                                               <  8:205368/130‑1 (MQ=255)                    aaCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCg                                                                                                                            >  4:173217/1‑149 (MQ=255)                    aaCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCCTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCCCTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaatggc                                                                                                                             >  4:384215/1‑143 (MQ=21)                     aCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTCAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGt                                                                                                                           <  6:81900/149‑1 (MQ=255)                      cccGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGtt                                                                                                                          >  4:392767/1‑149 (MQ=255)                       ccGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGttt                                                                                                                         >  5:309946/1‑149 (MQ=255)                        cgcgcgGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCCCTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCgatgc                                                                                                                        <  3:384215/149‑5 (MQ=21)                        cgcgcgGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGtttt                                                                                                                        <  5:86045/149‑1 (MQ=255)                           gcgggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGc                                                                                                                     <  6:107461/149‑1 (MQ=255)                             gggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGGTAGCGCTCAAGGGGCGTTTTGGCCt                                                                                                                   >  5:41810/1‑149 (MQ=255)                             gggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCt                                                                                                                   <  4:188714/149‑1 (MQ=255)                                 ggcAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGtt                                                                                                                >  5:49371/1‑148 (MQ=255)                                 ggcAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGtt                                                                                                                >  8:31353/1‑148 (MQ=255)                                  gcagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGa                                                                                                              <  8:298845/149‑1 (MQ=255)                                  gcagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCATGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGa                                                                                                              >  1:179466/1‑149 (MQ=255)                                   cagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGAt                                                                                                             <  4:91998/149‑1 (MQ=255)                                    agcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATc                                                                                                            <  3:392767/149‑1 (MQ=255)                                    agcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATc                                                                                                            >  6:93957/1‑149 (MQ=255)                                       aCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTc                                                                                                         >  2:39692/1‑149 (MQ=255)                                        ccATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGGTAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCg                                                                                                        <  6:41810/149‑1 (MQ=255)                                         cATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGTAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGa                                                                                                       <  8:370384/149‑1 (MQ=255)                                           tAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGACGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATg                                                                                                     <  5:351053/149‑1 (MQ=255)                                               tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCg                                                                                                                            >  8:112238/1‑122 (MQ=21)                                               tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAAc                                                                                                  >  7:320493/1‑148 (MQ=255)                                               tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGGCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCg                                                                                                                            <  7:112238/122‑1 (MQ=21)                                                 tCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGTGGCGTTTTGGCCTGTTGATCTTCGATGAACAAg                                                                                               >  4:366865/1‑149 (MQ=255)                                                    cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaa                                                                                                                                 <  8:359492/112‑2 (MQ=25)                                                    cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaa                                                                                                                                 >  7:359492/1‑111 (MQ=25)                                                    cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGgcg                                                                                            <  7:31353/149‑1 (MQ=255)                                                         tGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCgg                                                                                         <  8:320493/147‑1 (MQ=255)                                                           gAAGAGCCCTGCCACCGCAGGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgac                                                                                     <  4:78741/149‑1 (MQ=255)                                                            aaGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgact                                                                                    >  4:387485/1‑149 (MQ=255)                                                               agCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgactgac                                                                                 <  6:68308/149‑1 (MQ=255)                                                                   cTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGc                                                                             >  6:14479/1‑149 (MQ=255)                                                                      ccACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCggg                                                                          <  2:179466/149‑1 (MQ=255)                                                                             aTGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGtgctg                                                                     >  7:116263/1‑147 (MQ=255)                                                                              tGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAg                                                                  <  1:131854/149‑1 (MQ=255)                                                                                gCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGc                                                                <  4:351117/149‑1 (MQ=255)                                                                                    tCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGCCAAGCGGGTGCTGCAGGCCGGt                                                            >  5:248333/1‑149 (MQ=255)                                                                                          tGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGAc                                                      >  3:100530/1‑149 (MQ=255)                                                                                          tGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGGGACTGACAAGCGGGTGCTGCAGGACGGTGGCGAc                                                      >  5:415148/1‑149 (MQ=255)                                                                                          tGCAACCAAGCACATACATTCCGCCGGATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGAc                                                      <  8:297465/149‑1 (MQ=255)                                                                                             aaCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGt                                                                                        >  1:329074/1‑112 (MQ=255)                                                                                             aaCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGt                                                                                        <  2:329074/112‑1 (MQ=255)                                                                                              aCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTc                                                  >  2:209744/1‑149 (MQ=255)                                                                                                 aaGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTgg                                               >  4:82382/1‑149 (MQ=255)                                                                                                  aGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTggg                                              <  8:300230/149‑1 (MQ=255)                                                                                                   gCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                             >  6:227403/1‑149 (MQ=255)                                                                                                     aCCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgtgt                                           <  6:309946/149‑1 (MQ=255)                                                                                                     aCCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgtgt                                           >  7:20345/1‑149 (MQ=255)                                                                                                      ccTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTgg                                                          <  2:290396/133‑1 (MQ=255)                                                                                                      ccTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGtgtg                                          <  1:77659/149‑1 (MQ=255)                                                                                                       cTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGtgtg                                          >  8:261867/1‑148 (MQ=255)                                                                                                        tACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGa                                        >  2:351635/1‑149 (MQ=255)                                                                                                         aCATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGAt                                       >  1:295932/1‑149 (MQ=255)                                                                                                          cATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATc                                      >  3:163167/1‑149 (MQ=255)                                                                                                          cATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCACGCCGGTGGCGACTGTCTGGGTGTGGATc                                      <  1:231529/149‑1 (MQ=255)                                                                                                           aTTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                                     <  7:261867/149‑1 (MQ=255)                                                                                                           aTTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                                     <  4:203979/149‑1 (MQ=255)                                                                                                               cgccgcATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGgc                                  <  5:14479/148‑1 (MQ=255)                                                                                                                         ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                             <  1:239826/127‑1 (MQ=255)                                                                                                                         ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                             >  2:239826/1‑127 (MQ=255)                                                                                                                         ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacc                        >  8:291703/1‑148 (MQ=255)                                                                                                                         ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacc                        >  7:322733/1‑148 (MQ=255)                                                                                                                           aTTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacctac                     <  8:20345/149‑1 (MQ=255)                                                                                                                               gAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCAt                  <  3:173217/148‑1 (MQ=255)                                                                                                                                aGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCAta                 <  1:209744/148‑2 (MQ=255)                                                                                                                                aGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATgg                >  3:251323/1‑149 (MQ=255)                                                                                                                                 gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc               <  6:49371/149‑1 (MQ=255)                                                                                                                                 gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc               >  2:243257/1‑149 (MQ=255)                                                                                                                                 gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc               <  3:387485/149‑1 (MQ=255)                                                                                                                                     cGACAAGGCTGAAACCTTGTTTGCGCTCAAGGGGCGTTTTGGCCTGTTGAGCTTCGATGAACAATGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtgg           <  8:376001/149‑1 (MQ=255)                                                                                                                                      gACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTg                             >  4:386799/1‑130 (MQ=255)                                                                                                                                       aCAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtggtg         <  2:404251/149‑1 (MQ=255)                                                                                                                                       aCAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtggtg         <  3:82382/149‑1 (MQ=255)                                                                                                                                        catggCTGAAACCTTTATAGCGCTCATGGGCCGTTTTGGCCTGTTGATCTTCTATGAACAAGGCGCGGTGACTGACTCGGGGGTGCTGCAGGCCGGTGGGGACTGTGTGGGTGTGGTTCTGGCGCGTGGAACCTACCATGGCTtggggg        <  8:116263/146‑1 (MQ=255)                                                                                                                                            gCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtggtg         <  4:389830/144‑1 (MQ=255)                                                                                                                                            gCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtggtg         >  3:389830/1‑144 (MQ=255)                                                                                                                                             cTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTg   <  2:18284/149‑1 (MQ=255)                                                                                                                                              tGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                                     >  7:29063/1‑114 (MQ=255)                                                                                                                                              tGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                                     <  8:29063/114‑1 (MQ=255)                                                                                                                                               gAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTgg                                                          <  4:31828/92‑1 (MQ=255)                                                                                                                                               gAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTgg                                                          >  3:31828/1‑92 (MQ=255)                                                                                                                                               gAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTg   >  3:298618/1‑147 (MQ=255)                                                                                                                                                aaaCCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTgg  <  5:227403/147‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   GGCCGAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTGG  >  NC_002947/866592‑866881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑