Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 866,744 T→C 6.4% I66T (ATA→ACA) ‡ PP_0748 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947866,7440TC 6.4% 279.2 / 6.1 93I66T (ATA→ACA) ‡PP_0748hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (40/47);  new base C (3/3);  total (43/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTGGAGCCTGACAGC  >  NC_002947/866596‑866892
                                                                                                                                                    |                                                                                                                                                    
gAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGAt                                                                                                                                                      <  5:183201/149‑1 (MQ=255)
     ggCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAgcgc                                                                                                                                                 <  2:130701/149‑1 (MQ=255)
        cGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTc                                                                                                                                               >  3:91998/1‑148 (MQ=255)
         gCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCaa                                                                                                                                             <  5:289752/149‑1 (MQ=255)
            gCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAggg                                                                                                                                          <  8:158695/149‑1 (MQ=255)
            gCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAggg                                                                                                                                          >  1:404251/1‑149 (MQ=255)
              cAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGc                                                                                                                                        >  6:86045/1‑149 (MQ=255)
              cAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGc                                                                                                                                        >  2:77659/1‑149 (MQ=255)
               aaCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCg                                                                                                                                       >  4:173217/1‑149 (MQ=255)
               aaCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCCTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCCCTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaatggc                                                                                                                                        >  4:384215/1‑143 (MQ=21)
                aCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTCAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGt                                                                                                                                      <  6:81900/149‑1 (MQ=255)
                 cccGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGtt                                                                                                                                     >  4:392767/1‑149 (MQ=255)
                  ccGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGttt                                                                                                                                    >  5:309946/1‑149 (MQ=255)
                   cgcgcgGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCCCTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCgatgc                                                                                                                                   <  3:384215/149‑5 (MQ=21)
                   cgcgcgGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGtttt                                                                                                                                   <  5:86045/149‑1 (MQ=255)
                      gcgggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGc                                                                                                                                <  6:107461/149‑1 (MQ=255)
                        gggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGGTAGCGCTCAAGGGGCGTTTTGGCCt                                                                                                                              >  5:41810/1‑149 (MQ=255)
                        gggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCt                                                                                                                              <  4:188714/149‑1 (MQ=255)
                            ggcAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGtt                                                                                                                           >  8:31353/1‑148 (MQ=255)
                            ggcAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGtt                                                                                                                           >  5:49371/1‑148 (MQ=255)
                             gcagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGa                                                                                                                         <  8:298845/149‑1 (MQ=255)
                             gcagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCATGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGa                                                                                                                         >  1:179466/1‑149 (MQ=255)
                              cagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGAt                                                                                                                        <  4:91998/149‑1 (MQ=255)
                               agcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATc                                                                                                                       >  6:93957/1‑149 (MQ=255)
                               agcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATc                                                                                                                       <  3:392767/149‑1 (MQ=255)
                                  aCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTc                                                                                                                    >  2:39692/1‑149 (MQ=255)
                                   ccATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGGTAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCg                                                                                                                   <  6:41810/149‑1 (MQ=255)
                                    cATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGTAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGa                                                                                                                  <  8:370384/149‑1 (MQ=255)
                                      tAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGACGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATg                                                                                                                <  5:351053/149‑1 (MQ=255)
                                          tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCg                                                                                                                                       >  8:112238/1‑122 (MQ=21)
                                          tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAAc                                                                                                             >  7:320493/1‑148 (MQ=255)
                                          tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGGCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCg                                                                                                                                       <  7:112238/122‑1 (MQ=21)
                                            tCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGTGGCGTTTTGGCCTGTTGATCTTCGATGAACAAg                                                                                                          >  4:366865/1‑149 (MQ=255)
                                               cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaa                                                                                                                                            >  7:359492/1‑111 (MQ=25)
                                               cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaa                                                                                                                                            <  8:359492/112‑2 (MQ=25)
                                               cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGgcg                                                                                                       <  7:31353/149‑1 (MQ=255)
                                                    tGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCgg                                                                                                    <  8:320493/147‑1 (MQ=255)
                                                      gAAGAGCCCTGCCACCGCAGGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgac                                                                                                <  4:78741/149‑1 (MQ=255)
                                                       aaGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgact                                                                                               >  4:387485/1‑149 (MQ=255)
                                                          agCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgactgac                                                                                            <  6:68308/149‑1 (MQ=255)
                                                              cTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGc                                                                                        >  6:14479/1‑149 (MQ=255)
                                                                 ccACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCggg                                                                                     <  2:179466/149‑1 (MQ=255)
                                                                        aTGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGtgctg                                                                                >  7:116263/1‑147 (MQ=255)
                                                                         tGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAg                                                                             <  1:131854/149‑1 (MQ=255)
                                                                           gCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGc                                                                           <  4:351117/149‑1 (MQ=255)
                                                                               tCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGCCAAGCGGGTGCTGCAGGCCGGt                                                                       >  5:248333/1‑149 (MQ=255)
                                                                                     tGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGAc                                                                 >  3:100530/1‑149 (MQ=255)
                                                                                     tGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGGGACTGACAAGCGGGTGCTGCAGGACGGTGGCGAc                                                                 >  5:415148/1‑149 (MQ=255)
                                                                                     tGCAACCAAGCACATACATTCCGCCGGATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGAc                                                                 <  8:297465/149‑1 (MQ=255)
                                                                                        aaCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGt                                                                                                   <  2:329074/112‑1 (MQ=255)
                                                                                        aaCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGt                                                                                                   >  1:329074/1‑112 (MQ=255)
                                                                                         aCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTc                                                             >  2:209744/1‑149 (MQ=255)
                                                                                            aaGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTgg                                                          >  4:82382/1‑149 (MQ=255)
                                                                                             aGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTggg                                                         <  8:300230/149‑1 (MQ=255)
                                                                                              gCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                                        >  6:227403/1‑149 (MQ=255)
                                                                                                aCCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgtgt                                                      >  7:20345/1‑149 (MQ=255)
                                                                                                aCCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgtgt                                                      <  6:309946/149‑1 (MQ=255)
                                                                                                 ccTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTgg                                                                     <  2:290396/133‑1 (MQ=255)
                                                                                                 ccTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGtgtg                                                     <  1:77659/149‑1 (MQ=255)
                                                                                                  cTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGtgtg                                                     >  8:261867/1‑148 (MQ=255)
                                                                                                   tACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGa                                                   >  2:351635/1‑149 (MQ=255)
                                                                                                    aCATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGAt                                                  >  1:295932/1‑149 (MQ=255)
                                                                                                     cATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATc                                                 >  3:163167/1‑149 (MQ=255)
                                                                                                     cATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCACGCCGGTGGCGACTGTCTGGGTGTGGATc                                                 <  1:231529/149‑1 (MQ=255)
                                                                                                      aTTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                                                <  4:203979/149‑1 (MQ=255)
                                                                                                      aTTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                                                <  7:261867/149‑1 (MQ=255)
                                                                                                          cgccgcATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGgc                                             <  5:14479/148‑1 (MQ=255)
                                                                                                                    ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                                        <  1:239826/127‑1 (MQ=255)
                                                                                                                    ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                                        >  2:239826/1‑127 (MQ=255)
                                                                                                                    ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacc                                   >  7:322733/1‑148 (MQ=255)
                                                                                                                    ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacc                                   >  8:291703/1‑148 (MQ=255)
                                                                                                                      aTTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacctac                                <  8:20345/149‑1 (MQ=255)
                                                                                                                          gAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCAt                             <  3:173217/148‑1 (MQ=255)
                                                                                                                           aGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCAta                            <  1:209744/148‑2 (MQ=255)
                                                                                                                           aGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATgg                           >  3:251323/1‑149 (MQ=255)
                                                                                                                            gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc                          >  2:243257/1‑149 (MQ=255)
                                                                                                                            gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc                          <  6:49371/149‑1 (MQ=255)
                                                                                                                            gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc                          <  3:387485/149‑1 (MQ=255)
                                                                                                                                cGACAAGGCTGAAACCTTGTTTGCGCTCAAGGGGCGTTTTGGCCTGTTGAGCTTCGATGAACAATGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtgg                      <  8:376001/149‑1 (MQ=255)
                                                                                                                                 gACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTg                                        >  4:386799/1‑130 (MQ=255)
                                                                                                                                  aCAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtggtg                    <  2:404251/149‑1 (MQ=255)
                                                                                                                                  aCAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtggtg                    <  3:82382/149‑1 (MQ=255)
                                                                                                                                   catggCTGAAACCTTTATAGCGCTCATGGGCCGTTTTGGCCTGTTGATCTTCTATGAACAAGGCGCGGTGACTGACTCGGGGGTGCTGCAGGCCGGTGGGGACTGTGTGGGTGTGGTTCTGGCGCGTGGAACCTACCATGGCTtggggg                   <  8:116263/146‑1 (MQ=255)
                                                                                                                                       gCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtggtg                    >  3:389830/1‑144 (MQ=255)
                                                                                                                                       gCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtggtg                    <  4:389830/144‑1 (MQ=255)
                                                                                                                                        cTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTg              <  2:18284/149‑1 (MQ=255)
                                                                                                                                         tGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                                                >  7:29063/1‑114 (MQ=255)
                                                                                                                                         tGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                                                <  8:29063/114‑1 (MQ=255)
                                                                                                                                          gAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTgg                                                                     <  4:31828/92‑1 (MQ=255)
                                                                                                                                          gAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTgg                                                                     >  3:31828/1‑92 (MQ=255)
                                                                                                                                          gAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTg              >  3:298618/1‑147 (MQ=255)
                                                                                                                                           aaaCCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTgg             <  5:227403/147‑1 (MQ=255)
                                                                                                                                            aaCCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTGGAGc          <  7:291703/149‑1 (MQ=255)
                                                                                                                                            aaCCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTGGAGc          <  4:100530/149‑1 (MQ=255)
                                                                                                                                                    tAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCt                                         >  1:263621/1‑110 (MQ=255)
                                                                                                                                                    tAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCt                                         <  2:263621/110‑1 (MQ=255)
                                                                                                                                                    tAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTGGAGCCTGACAGc  <  4:251323/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAAAAGGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTGGAGCCTGACAGC  >  NC_002947/866596‑866892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑