Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	866,745	A→G	6.3%	I66M (ATA→ATG) ‡	PP_0748 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	866,745	0	A	G	6.3%	288.8 / 5.7	96	I66M (ATA→ATG) ‡	PP_0748	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (41/48);  new base G (3/3);  total (44/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTGGAGCCTGACAGC  >  NC_002947/866601‑866892                                                                                                                                                 |                                                                                                                                                    ggCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAgcgc                                                                                                                                                 <  2:130701/149‑1 (MQ=255)    cGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTc                                                                                                                                               >  3:91998/1‑148 (MQ=255)     gCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCaa                                                                                                                                             <  5:289752/149‑1 (MQ=255)        gCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAggg                                                                                                                                          <  8:158695/149‑1 (MQ=255)        gCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAggg                                                                                                                                          >  1:404251/1‑149 (MQ=255)          cAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGc                                                                                                                                        >  2:77659/1‑149 (MQ=255)          cAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGc                                                                                                                                        >  6:86045/1‑149 (MQ=255)           aaCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCg                                                                                                                                       >  4:173217/1‑149 (MQ=255)           aaCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCCTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCCCTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaatggc                                                                                                                                        >  4:384215/1‑143 (MQ=21)            aCCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTCAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGt                                                                                                                                      <  6:81900/149‑1 (MQ=255)             cccGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGtt                                                                                                                                     >  4:392767/1‑149 (MQ=255)              ccGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGttt                                                                                                                                    >  5:309946/1‑149 (MQ=255)               cgcgcgGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCCCTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCgatgc                                                                                                                                   <  3:384215/149‑5 (MQ=21)               cgcgcgGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGtttt                                                                                                                                   <  5:86045/149‑1 (MQ=255)                  gcgggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGc                                                                                                                                <  6:107461/149‑1 (MQ=255)                    gggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGGTAGCGCTCAAGGGGCGTTTTGGCCt                                                                                                                              >  5:41810/1‑149 (MQ=255)                    gggcggCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCt                                                                                                                              <  4:188714/149‑1 (MQ=255)                        ggcAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGtt                                                                                                                           >  8:31353/1‑148 (MQ=255)                        ggcAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGtt                                                                                                                           >  5:49371/1‑148 (MQ=255)                         gcagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGa                                                                                                                         <  8:298845/149‑1 (MQ=255)                         gcagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCATGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGa                                                                                                                         >  1:179466/1‑149 (MQ=255)                          cagcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGAt                                                                                                                        <  4:91998/149‑1 (MQ=255)                           agcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATc                                                                                                                       >  6:93957/1‑149 (MQ=255)                           agcaCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATc                                                                                                                       <  3:392767/149‑1 (MQ=255)                              aCCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTc                                                                                                                    >  2:39692/1‑149 (MQ=255)                               ccATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGGTAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCg                                                                                                                   <  6:41810/149‑1 (MQ=255)                                cATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGTAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGa                                                                                                                  <  8:370384/149‑1 (MQ=255)                                  tAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGACGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATg                                                                                                                <  5:351053/149‑1 (MQ=255)                                      tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCg                                                                                                                                       >  8:112238/1‑122 (MQ=21)                                      tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAAc                                                                                                             >  7:320493/1‑148 (MQ=255)                                      tttCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGGCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCAAATGGCg                                                                                                                                       <  7:112238/122‑1 (MQ=21)                                        tCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGTGGCGTTTTGGCCTGTTGATCTTCGATGAACAAg                                                                                                          >  4:366865/1‑149 (MQ=255)                                           cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaa                                                                                                                                            >  7:359492/1‑111 (MQ=25)                                           cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCTATCAAGGTTTCAGCCTTGTCGGCGCTCaaa                                                                                                                                            <  8:359492/112‑2 (MQ=25)                                           cGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGgcg                                                                                                       <  7:31353/149‑1 (MQ=255)                                                tGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCgg                                                                                                    <  8:320493/147‑1 (MQ=255)                                                  gAAGAGCCCTGCCACCGCAGGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgac                                                                                                <  4:78741/149‑1 (MQ=255)                                                   aaGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgact                                                                                               >  4:387485/1‑149 (MQ=255)                                                      agCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGtgactgac                                                                                            <  6:68308/149‑1 (MQ=255)                                                          cTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGc                                                                                        >  6:14479/1‑149 (MQ=255)                                                             ccACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCggg                                                                                     <  2:179466/149‑1 (MQ=255)                                                                    aTGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGtgctg                                                                                >  7:116263/1‑147 (MQ=255)                                                                     tGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAg                                                                             <  1:131854/149‑1 (MQ=255)                                                                       gCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGc                                                                           <  4:351117/149‑1 (MQ=255)                                                                           tCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGCCAAGCGGGTGCTGCAGGCCGGt                                                                       >  5:248333/1‑149 (MQ=255)                                                                                 tGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGAc                                                                 >  3:100530/1‑149 (MQ=255)                                                                                 tGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGGGACTGACAAGCGGGTGCTGCAGGACGGTGGCGAc                                                                 >  5:415148/1‑149 (MQ=255)                                                                                 tGCAACCAAGCACATACATTCCGCCGGATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGAc                                                                 <  8:297465/149‑1 (MQ=255)                                                                                    aaCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGt                                                                                                   >  1:329074/1‑112 (MQ=255)                                                                                    aaCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGt                                                                                                   <  2:329074/112‑1 (MQ=255)                                                                                     aCCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTc                                                             >  2:209744/1‑149 (MQ=255)                                                                                        aaGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTgg                                                          >  4:82382/1‑149 (MQ=255)                                                                                         aGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTggg                                                         <  8:300230/149‑1 (MQ=255)                                                                                          gCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                                        >  6:227403/1‑149 (MQ=255)                                                                                            aCCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgtgt                                                      >  7:20345/1‑149 (MQ=255)                                                                                            aCCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgtgt                                                      <  6:309946/149‑1 (MQ=255)                                                                                             ccTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTgg                                                                     <  2:290396/133‑1 (MQ=255)                                                                                             ccTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGtgtg                                                     <  1:77659/149‑1 (MQ=255)                                                                                              cTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGtgtg                                                     >  8:261867/1‑148 (MQ=255)                                                                                               tACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGa                                                   >  2:351635/1‑149 (MQ=255)                                                                                                aCATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGAt                                                  >  1:295932/1‑149 (MQ=255)                                                                                                 cATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATc                                                 >  3:163167/1‑149 (MQ=255)                                                                                                 cATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCACGCCGGTGGCGACTGTCTGGGTGTGGATc                                                 <  1:231529/149‑1 (MQ=255)                                                                                                  aTTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                                                <  4:203979/149‑1 (MQ=255)                                                                                                  aTTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                                                <  7:261867/149‑1 (MQ=255)                                                                                                      cgccgcATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGgc                                             <  5:14479/148‑1 (MQ=255)                                                                                                                ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                                        <  1:239826/127‑1 (MQ=255)                                                                                                                ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGgt                                                        >  2:239826/1‑127 (MQ=255)                                                                                                                ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacc                                   >  8:291703/1‑148 (MQ=255)                                                                                                                ccATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacc                                   >  7:322733/1‑148 (MQ=255)                                                                                                                  aTTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGtacctac                                <  8:20345/149‑1 (MQ=255)                                                                                                                      gAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCAt                             <  3:173217/148‑1 (MQ=255)                                                                                                                       aGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCAta                            <  1:209744/148‑2 (MQ=255)                                                                                                                       aGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATgg                           >  3:251323/1‑149 (MQ=255)                                                                                                                        gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc                          <  6:49371/149‑1 (MQ=255)                                                                                                                        gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc                          >  2:243257/1‑149 (MQ=255)                                                                                                                        gcgcCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGc                          <  3:387485/149‑1 (MQ=255)                                                                                                                            cGACAAGGCTGAAACCTTGTTTGCGCTCAAGGGGCGTTTTGGCCTGTTGAGCTTCGATGAACAATGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtgg                      <  8:376001/149‑1 (MQ=255)                                                                                                                             gACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTg                                        >  4:386799/1‑130 (MQ=255)                                                                                                                              aCAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtggtg                    <  3:82382/149‑1 (MQ=255)                                                                                                                              aCAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtggtg                    <  2:404251/149‑1 (MQ=255)                                                                                                                               catggCTGAAACCTTTATAGCGCTCATGGGCCGTTTTGGCCTGTTGATCTTCTATGAACAAGGCGCGGTGACTGACTCGGGGGTGCTGCAGGCCGGTGGGGACTGTGTGGGTGTGGTTCTGGCGCGTGGAACCTACCATGGCTtggggg                   <  8:116263/146‑1 (MQ=255)                                                                                                                                   gCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtggtg                    <  4:389830/144‑1 (MQ=255)                                                                                                                                   gCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTtggtg                    >  3:389830/1‑144 (MQ=255)                                                                                                                                    cTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTg              <  2:18284/149‑1 (MQ=255)                                                                                                                                     tGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                                                >  7:29063/1‑114 (MQ=255)                                                                                                                                     tGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCt                                                <  8:29063/114‑1 (MQ=255)                                                                                                                                      gAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTgg                                                                     <  4:31828/92‑1 (MQ=255)                                                                                                                                      gAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTgg                                                                     >  3:31828/1‑92 (MQ=255)                                                                                                                                      gAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTg              >  3:298618/1‑147 (MQ=255)                                                                                                                                       aaaCCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTgg             <  5:227403/147‑1 (MQ=255)                                                                                                                                        aaCCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTGGAGc          <  4:100530/149‑1 (MQ=255)                                                                                                                                        aaCCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTGGAGc          <  7:291703/149‑1 (MQ=255)                                                                                                                                                tAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCt                                         <  2:263621/110‑1 (MQ=255)                                                                                                                                                tAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCt                                         >  1:263621/1‑110 (MQ=255)                                                                                                                                                tAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTGGAGCCTGACAGc  <  4:251323/149‑1 (MQ=255)                                                                                                                                                 aGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTGGAGCCTGAc     >  6:86098/1‑145 (MQ=255)                                                                                                                                                 aGCCCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTGGAGCCTGAc     <  5:86098/145‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    GGCCGCTGCCAACCCGCGCGGGCGGCAGCACCATAACTTTCACGCTATGGAAGAGCCCTGCCACCGCATGGCCGTCGGGCTGCAACCAAGCACCTACATTCCGCCGCATCGCCATTTGAGCGCCGACAAGGCTGAAACCTTGATAGCGCTCAAGGGGCGTTTTGGCCTGTTGATCTTCGATGAACAAGGCGCGGTGACTGACAAGCGGGTGCTGCAGGCCGGTGGCGACTGTCTGGGTGTGGATCTGGCGCCTGGTACCTACCATGGCTTGGTGGTGCTGGAGCCTGACAGC  >  NC_002947/866601‑866892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑