Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	900,289	T→A	6.1%	L133L (CTT→CTA)	PP_0784 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	900,289	0	T	A	6.1%	197.0 / 2.8	65	L133L (CTT→CTA)	PP_0784	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/29);  new base A (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

TCGCTCGCGGCGCATTTGGTGACCTGTATCTCTTTGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCAAGCGACCTTGCTAACCTCGAGAAGGT  >  NC_002947/900141‑900435                                                                                                                                                     |                                                                                                                                                    tcgctcgcGGCTCATTTGGTGACCTGTATCTCTTTGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTGACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCtt                                                                                                                                                     <  7:135828/149‑1 (MQ=255) tcgctcgcGGCGCATTTGGTGACCTGTATCTCTTTGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCtt                                                                                                                                                     >  3:167913/1‑149 (MQ=255)   gctcgcGGCGCATTTGGTGACCTGTATCTCTTTGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTg                                                                                                                                                    >  5:243634/1‑148 (MQ=255)      cgcgGCGCATTTGGTGACCTGTATCTCTTTGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCAGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCa                                                                                                                                                 >  4:267917/1‑148 (MQ=255)       gcggcgCATTTGGTGACCTGTATCTCTTTGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCTGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCaaa                                                                                                                                               <  3:267917/149‑1 (MQ=255)            gcATTTGGTGACCTGTATCTCTTTGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGaa                                                                                                                                          <  3:218411/149‑1 (MQ=255)                        cTGTATCTCTTTGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGa                                                                                                                              >  2:252951/1‑149 (MQ=255)                          gTATCTCTTTGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACt                                                                                                                            >  3:195047/1‑149 (MQ=255)                           tATCTCTTTGGAGAGCAAGCGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGAc                                                                                                                             <  1:222542/147‑1 (MQ=255)                            aTCTCTTTGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACttt                                                                                                                          >  2:94100/1‑149 (MQ=255)                               tctTTGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGAt                                                                                                                       >  5:182115/1‑149 (MQ=255)                                ctTTGGAGAGGAAACGGTTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGAGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGATAAGGCGTCTGTTGACTTTGAt                                                                                                                       <  7:77619/148‑1 (MQ=255)                                ctTTGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATg                                                                                                                      >  1:112096/1‑149 (MQ=255)                                 tttGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGAt                                                                                                                       <  2:230684/147‑1 (MQ=255)                                   ttgagaGCATACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATg                                                                                                                      <  6:243634/144‑1 (MQ=255)                                   tGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATg                                                                                                                      <  2:112096/146‑1 (MQ=255)                                     gagaGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATg                                                                                                                 >  3:253038/1‑149 (MQ=255)                                                   ttCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCa                                                                                                     >  3:189527/1‑147 (MQ=255)                                                       cAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAg                                                                                                                                         >  3:146750/1‑107 (MQ=255)                                                       cAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAg                                                                                                                                         <  4:146750/107‑1 (MQ=255)                                                       cAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAaag                                                                                                  >  2:234097/1‑146 (MQ=255)                                                        aGTAAAGATTTTGGCTCATTTTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAaagaa                                                                                                <  3:309105/147‑1 (MQ=255)                                                        aGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAaaga                                                                                                 <  3:158316/146‑1 (MQ=255)                                                        aGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAagaaga                                                                                              <  4:195047/149‑1 (MQ=255)                                                          tAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGaaa                                                                                            >  2:83215/1‑149 (MQ=255)                                                            aaGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTAGGCAAAGAGAAGGCGTCTGTTGAc                                                                                                                             <  8:42099/114‑1 (MQ=255)                                                            aaGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTAGGCAAAGAGAAGGCGTCTGTTGAc                                                                                                                             >  7:35868/1‑114 (MQ=255)                                                            aaGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTAGGCAAAGAGAAGGCGTCTGTTGAc                                                                                                                             >  7:42099/1‑114 (MQ=255)                                                            aaGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTAGGCAAAGAGAAGGCGTCTGTTGAc                                                                                                                             <  8:35868/114‑1 (MQ=255)                                                                  ttGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCa                                                                                    <  1:252951/148‑1 (MQ=255)                                                                         atatTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGTGAAGTGCAAGGTTTCTTCGTTGGCAAAGATAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATTCTAAAg                                                                            >  5:333266/1‑149 (MQ=255)                                                                         atatTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTaaa                                                                             <  2:91698/148‑1 (MQ=255)                                                                         atatTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAg                                                                            <  8:100227/149‑1 (MQ=255)                                                                          tatTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCTAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGc                                                                           <  6:182115/149‑1 (MQ=255)                                                                               cACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCatga                                                                       <  3:56183/148‑1 (MQ=255)                                                                                  gATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCatga                                                                       <  7:95564/145‑1 (MQ=255)                                                                                  gATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCatga                                                                       >  8:95564/1‑145 (MQ=255)                                                                                     aCAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGGAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATg                                                                >  3:20825/1‑149 (MQ=255)                                                                                       aGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTa                                                              >  2:193572/1‑149 (MQ=255)                                                                                         gggCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATg                                                            <  4:189527/149‑1 (MQ=255)                                                                                            cAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATg                                                            <  1:69189/146‑1 (MQ=255)                                                                                            cAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGgt                                                         >  7:387029/1‑149 (MQ=255)                                                                                            cAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCATTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATg                                                            >  2:69189/1‑146 (MQ=255)                                                                                                      gAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATg                                                                <  1:25425/132‑1 (MQ=255)                                                                                                      gAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATg                                                                >  2:25425/1‑132 (MQ=255)                                                                                                          tGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCtt                                            <  1:193572/148‑1 (MQ=255)                                                                                                           gACGGCGGCGGATATGGACAGGGAAGTGGAAGGTTTCTTCTTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGGCCGCAAAGAAGTAATTG‑GGCATGCTAAAGCATGATGTAATGTATGGGTTCGTTCCAGCCTTg                                           <  6:333266/148‑1 (MQ=255)                                                                                                           gACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTgg                                          >  7:333347/1‑149 (MQ=255)                                                                                                            aCGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTGCGGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTg                                           <  1:83215/148‑1 (MQ=255)                                                                                                                     gATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTcggcgg                                <  8:333347/149‑1 (MQ=255)                                                                                                                         tGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCa                            <  7:35667/149‑1 (MQ=255)                                                                                                                           gACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCAAg                          >  6:128788/1‑149 (MQ=255)                                                                                                                              aGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCAAGCGa                       <  4:253038/149‑1 (MQ=255)                                                                                                                                ggAAGTGAAAGGTTTCTTCATTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCTCAAAGAAGAAAATG‑TGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGCCGTTCGACGGGCCAAGCGAcc                     >  4:296246/1‑149 (MQ=255)                                                                                                                                   aGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCAAGCGACCTTg                  <  1:234097/149‑1 (MQ=255)                                                                                                                                      gCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCAAGCGACCTTGCt                >  5:187657/1‑148 (MQ=255)                                                                                                                                       cAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGGGTTCGGCGGGTCAAGCGACCTTGCTaa              >  1:340674/1‑149 (MQ=255)                                                                                                                                       cAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCAAGCGACCTTGCTaa              >  5:11094/1‑149 (MQ=255)                                                                                                                                       cAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCAAGCGACCTTGCTaa              >  7:295055/1‑149 (MQ=255)                                                                                                                                       cAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGAGGGTCAAGCGACCTTGCTaa              >  7:198423/1‑149 (MQ=255)                                                                                                                                        aaGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAATTGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTGAAGCGACCTTGCTAAc             >  8:360298/1‑149 (MQ=255)                                                                                                                                        aaGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCAAGCGACCTTGCTAAc             >  6:120374/1‑149 (MQ=255)                                                                                                                                               cttcCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCAAGCGACCTTGCTAACCTCgag       <  5:128788/148‑1 (MQ=255)                                                                                                                                                ttcCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCAAGCGACCTTGCTAACCTCgag       >  4:158984/1‑147 (MQ=255)                                                                                                                                                  ccTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCAACCGACCTTGCTAACCTCGAGAAgg   >  7:116253/1‑149 (MQ=255)                                                                                                                                                   cTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCAAGCGACCTTGCTAACCTCGAGAAgg   <  3:96250/148‑1 (MQ=255)                                                                                                                                                   cTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCAAGCGACCTTGCTAACCTCGAGAAGGt  >  2:25942/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TCGCTCGCGGCGCATTTGGTGACCTGTATCTCTTTGGAGAGCAAACGGGTTTCTCAGTAAAGATTTTGGCTCATATTTCACGATACAGGGGCAGTGAATATGAGTTGACGGCGGCGGATATGGACAGGGAAGTGCAAGGTTTCTTCCTTGGCAAAGAGAAGGCGTCTGTTGACTTTGATGGTATGTTCGAGCCCGCAAAGAAGAAATTG‑GGCATGCTAAAGCATGATGAAATGTATGGGTTCGTTCCAGCCTTGGCGTTCGGCGGGTCAAGCGACCTTGCTAACCTCGAGAAGGT  >  NC_002947/900141‑900435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑