Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 925,902 A→G 7.1% W242R (TGG→CGG) ‡ PP_0805 ← outer membrane efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947925,9020AG 7.1% 241.4 / 6.2 84W242R (TGG→CGG) ‡PP_0805outer membrane efflux protein
Reads supporting (aligned to +/- strand):  ref base A (40/38);  new base G (3/3);  total (43/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01

GCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGCCCACGCCGCGAATCT  >  NC_002947/925754‑926048
                                                                                                                                                    |                                                                                                                                                  
gcgcgcTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCa                                                                                                                                                    <  6:38383/149‑1 (MQ=255)
  gcgcTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAgc                                                                                                                                                  >  6:401094/1‑149 (MQ=255)
  gcgcTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAgc                                                                                                                                                  >  6:214821/1‑149 (MQ=255)
     cTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCt                                                                                                                                               <  8:190417/149‑1 (MQ=255)
          ccagggccaTCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTAc                                                                                                                                          >  7:141696/1‑149 (MQ=255)
          ccagggccaTCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTAc                                                                                                                                          >  8:340140/1‑149 (MQ=255)
               gccaTCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGGCGAGCGCCAGCGCTCGTACTGCGa                                                                                                                                     <  5:263847/149‑1 (MQ=255)
                  aTCAGCACGGCGGGCAGGCGATTATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTg                                                                                                                                  <  5:419253/149‑1 (MQ=255)
                   tCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTgg                                                                                                                                 <  8:277142/149‑1 (MQ=255)
                     aGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTa                                                                                                                               <  8:241272/149‑1 (MQ=255)
                           gcggGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCTGGCGCTCgacc                                                                                                                                          <  6:234183/132‑4 (MQ=255)
                               gCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAgg                                                                                                                     <  8:141696/149‑1 (MQ=255)
                                   gCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTgg                                                                                                                 >  3:13739/1‑149 (MQ=255)
                                     gATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCa                                                                                                               <  7:192575/149‑1 (MQ=255)
                                     gATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCa                                                                                                               >  4:137392/1‑149 (MQ=255)
                                        catcaCCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACgg                                                                                                            >  2:117269/1‑149 (MQ=255)
                                        catcaCCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACgg                                                                                                            >  3:303264/1‑149 (MQ=255)
                                         atcaCCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTActc                                                                                                                            <  6:129901/132‑1 (MQ=255)
                                         atcaCCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTActc                                                                                                                            >  5:129901/1‑132 (MQ=255)
                                         atcaCCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACggg                                                                                                           <  1:234375/149‑1 (MQ=255)
                                             cccTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCt                                                                                                       <  8:9981/149‑1 (MQ=255)
                                                tgtgCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTc                                                                                                    >  2:122573/1‑149 (MQ=255)
                                                tgtgCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTc                                                                                                    >  4:272536/1‑149 (MQ=255)
                                                    cAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCGTTCCAcg                                                                                                >  4:38757/1‑149 (MQ=255)
                                                     aGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCAcgc                                                                                               >  1:183340/1‑149 (MQ=255)
                                                          gcTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGa                                                                                                                                     <  5:128790/106‑1 (MQ=255)
                                                          gcTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGa                                                                                                                                     >  6:128790/1‑106 (MQ=255)
                                                               cAGGCCTGGGCCAGCTCGGCGGTTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTGCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGc                                                                                     <  7:221772/149‑1 (MQ=255)
                                                                aGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGcc                                                                                    <  5:129025/149‑1 (MQ=255)
                                                                   ccTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGt                                                                                 <  3:157417/149‑1 (MQ=255)
                                                                       gTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCa                                                                             >  5:67510/1‑149 (MQ=255)
                                                                       gTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCa                                                                             >  5:56695/1‑149 (MQ=255)
                                                                       gTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCA‑GGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCAc                                                                            >  7:351160/1‑149 (MQ=255)
                                                                          cAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCTCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGt                                                                          <  8:272167/149‑1 (MQ=255)
                                                                           aGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCAAGGGTGGCACGGGCGCTTTCAACGCGGGACTCGGCCTGTACCACGTc                                                                         >  1:79765/1‑149 (MQ=255)
                                                                               cggcggGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCgg                                                                       <  4:62959/147‑1 (MQ=255)
                                                                               cggcggGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCgg                                                                       >  3:62959/1‑147 (MQ=255)
                                                                                ggcggGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAAc                                                                    <  6:62901/149‑1 (MQ=255)
                                                                                  cggGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACgg                                                                  <  6:67510/149‑1 (MQ=255)
                                                                                  cggGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACgg                                                                  >  5:391351/1‑149 (MQ=255)
                                                                                    ggTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGt                                                                <  8:8258/149‑1 (MQ=255)
                                                                                     gTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGt                                                                                                                   <  5:138206/97‑1 (MQ=255)
                                                                                     gTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGt                                                                                                                   >  6:138206/1‑97 (MQ=255)
                                                                                            cTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGgcgc                                                        >  4:65730/1‑149 (MQ=255)
                                                                                             tCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTc                                                                                        >  4:86522/1‑116 (MQ=255)
                                                                                             tCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTc                                                                                        <  3:86522/116‑1 (MQ=255)
                                                                                               ggCCAGTGCCGTAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGcc                                                       <  7:340140/147‑1 (MQ=255)
                                                                                                gCCAGTGCCGGAGGGGGAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCAt                                                    <  3:272682/149‑1 (MQ=255)
                                                                                                  cAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTAc                                                                                                                              >  3:115667/1‑73 (MQ=255)
                                                                                                  cAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTAc                                                                                                                              <  4:115667/73‑1 (MQ=255)
                                                                                                  cAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGt                                                  >  2:340968/1‑149 (MQ=255)
                                                                                                   aGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTc                                                 >  3:378352/1‑149 (MQ=255)
                                                                                                    gTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTcg                                                >  7:285445/1‑149 (MQ=255)
                                                                                                         ggAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTcgctgcg                                           >  5:148285/1‑149 (MQ=255)
                                                                                                         ggAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTcgctgcg                                           >  5:133341/1‑149 (MQ=255)
                                                                                                           gatcatAACGCGCCCCATCAGCGACGCGAGGGGCGAGCGCCAGCGCTCGTACTGCGACTGGGACTCCTCCCGGGTGGCACGGGCGCTTACCACGCGGGACGCGGCCTGGACCACGGCGGACCGGGGGCTGGCGCCCATGTcgctgcgct                                         <  5:305957/144‑1 (MQ=255)
                                                                                                                gaaCGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGCTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCAGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCgg                                    <  4:212386/148‑1 (MQ=255)
                                                                                                                 aaCGCGACCCATCAGCGCCGCCAGGGTCGCGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCAAGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTcgctgcgct                                         >  6:305957/1‑143 (MQ=255)
                                                                                                                 aaCGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCggg                                   >  2:304019/1‑149 (MQ=255)
                                                                                                                  aCGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGc                                  >  1:221456/1‑149 (MQ=255)
                                                                                                                   cgcgACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGcc                                 <  5:390722/149‑1 (MQ=255)
                                                                                                                   cgcgACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGcc                                 <  5:384899/149‑1 (MQ=255)
                                                                                                                   cgcgACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGcc                                 <  1:117269/149‑1 (MQ=255)
                                                                                                                    gcgACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCa                                >  8:368035/1‑149 (MQ=255)
                                                                                                                     cgACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGcctt                               <  4:303264/149‑3 (MQ=255)
                                                                                                                      gACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTACGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATg                              >  8:33668/1‑149 (MQ=255)
                                                                                                                         ccATCAGCGCCGCCAGGGGCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCg                           <  5:401094/149‑1 (MQ=255)
                                                                                                                          cATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCgg                          <  2:183340/149‑1 (MQ=255)
                                                                                                                           aTCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGccc                                                      <  7:125590/120‑1 (MQ=255)
                                                                                                                           aTCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGccc                                                      >  8:125590/1‑120 (MQ=255)
                                                                                                                            tCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGc                         >  2:345161/1‑148 (MQ=255)
                                                                                                                             cAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGc                                  >  6:40074/1‑138 (MQ=255)
                                                                                                                             cAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGc                                  <  5:40074/138‑1 (MQ=255)
                                                                                                                              aGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAAt                      >  4:346704/1‑149 (MQ=255)
                                                                                                                                        agtacGAGCGCTGTCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGc                 <  2:218881/140‑1 (MQ=255)
                                                                                                                                        agtacGAGCGCTGGCGCTCGTACTGCTCCTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGc                 >  1:218881/5‑144 (MQ=255)
                                                                                                                                        agtacGAGCGCTGGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGc                 >  1:212284/5‑144 (MQ=255)
                                                                                                                                        agtacGAGCGCTGGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGCGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGc                 <  2:212284/140‑1 (MQ=255)
                                                                                                                                          tacGAGCGCTGGCGCTCGTACTGCGACAGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGCCCAcgcc          >  5:346696/3‑149 (MQ=255)
                                                                                                                                             gAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGCCTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGCCCAcgccgc        >  5:312773/1‑148 (MQ=255)
                                                                                                                                             gAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGCCCAcgccgc        >  3:182082/1‑148 (MQ=255)
                                                                                                                                              aGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGCCCACGCCGCGa      <  8:285445/149‑1 (MQ=255)
                                                                                                                                                 gcCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGCCCACGCCGCGAATc   <  2:232634/149‑1 (MQ=255)
                                                                                                                                                 gcCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGCCCACGCCGCGAATc   <  1:122573/149‑1 (MQ=255)
                                                                                                                                                  ccAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGCCCACGCCGCGAATCt  <  3:137392/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGCCCACGCCGCGAATCT  >  NC_002947/925754‑926048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑