Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 927,771 G→T 5.3% L5951M (CTG→ATG) ‡ PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947927,7710GT 5.3% 244.1 / 3.4 75L5951M (CTG→ATG) ‡PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (32/39);  new base T (2/2);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

AGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCGTACTGCTGCTGGAGTCCACGCGGTTG  >  NC_002947/927624‑927916
                                                                                                                                                    |                                                                                                                                                  
aggtaCCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAgg                                                                                                                                                   >  8:313523/1‑149 (MQ=255)
aggtaCCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAgg                                                                                                                                                   >  8:313669/1‑149 (MQ=255)
 ggtaCCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAgg                                                                                                                                                   <  5:281627/148‑1 (MQ=255)
 accgccGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAgg                                                                                                                                                   <  5:161476/144‑1 (MQ=255)
       ggCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCCTGCTCAAcg                                                                                                                                            <  2:395927/149‑2 (MQ=255)
               ggTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGt                                                                                                                                         >  1:256884/1‑144 (MQ=255)
               ggTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGt                                                                                                                                         <  2:256884/144‑1 (MQ=255)
                 ttCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGa                                                                                                                                  <  7:313669/149‑1 (MQ=255)
                 ttCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGa                                                                                                                                  <  7:313523/149‑1 (MQ=255)
                  tctcGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAg                                                                                                                                 >  5:405750/1‑149 (MQ=255)
                   ctcGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAg                                                                                                                                 <  2:226472/148‑1 (MQ=255)
                     cGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTg                                                                                                                              >  1:136744/1‑149 (MQ=255)
                      gAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGt                                                                                                                                <  3:296577/146‑1 (MQ=255)
                      gAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGa                                                                                                                             <  3:94785/149‑1 (MQ=255)
                       aaGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGac                                                                                                                            <  7:106946/149‑1 (MQ=255)
                           tataCCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGaccacc                                                                                                                        <  5:30132/149‑1 (MQ=255)
                           tataCCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGaccacc                                                                                                                        <  2:136744/149‑1 (MQ=255)
                            ataCCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGaccacc‑a                                                                                                                      >  8:375887/1‑149 (MQ=255)
                               cccGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCt                                                                                                                   <  2:56806/149‑1 (MQ=255)
                                        gccgccCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCgg                                                                                                          >  6:103860/1‑149 (MQ=255)
                                              cAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTtg                                                                                                                                       <  5:294327/115‑1 (MQ=255)
                                              cAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTtg                                                                                                                                       >  6:294327/1‑115 (MQ=255)
                                              cAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTgg                                                                                                    <  3:320953/149‑1 (MQ=255)
                                               aGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGt                                                                                                   <  3:157964/149‑1 (MQ=255)
                                                     cTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTggc                                                                                             >  3:103112/1‑149 (MQ=255)
                                                      tGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGTTCGGCCGTGGTATTggcg                                                                                            >  4:75246/1‑149 (MQ=255)
                                                      tGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTggcg                                                                                            >  2:357868/1‑149 (MQ=255)
                                                      tGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTggcg                                                                                            >  7:308830/1‑149 (MQ=255)
                                                      tGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTggcg                                                                                            >  6:239611/1‑149 (MQ=255)
                                                       gACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTg                                                                                                     >  7:139457/1‑139 (MQ=255)
                                                       gACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTg                                                                                                     <  8:139457/139‑1 (MQ=255)
                                                        aCCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTggcgg                                                                                           >  7:42652/1‑148 (MQ=255)
                                                         ccAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTggcggc                                                                                          >  6:63807/1‑148 (MQ=255)
                                                                  caccTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGGCGGCCGTGGTATTGGCGGCAACCgtggtg                                                                                <  2:296846/149‑1 (MQ=255)
                                                                  caccTGGCACCGACTGTACCAACACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAg                                                                                                                                           >  7:180361/1‑91 (MQ=255)
                                                                  caccTGGCACCGACTGTACCAACACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAg                                                                                                                                           <  8:180361/91‑1 (MQ=255)
                                                                   accTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCgtggtgg                                                                               >  2:18146/1‑149 (MQ=255)
                                                                        gCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGATCACC‑ACCTGCAGGTCGGCCGt                                                                                                      <  1:136906/121‑1 (MQ=255)
                                                                        gCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGATCACC‑ACCTGCAGGTCGGCCGt                                                                                                      >  2:136906/1‑121 (MQ=255)
                                                                        gCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAg                                                                          >  1:210350/1‑149 (MQ=255)
                                                                         cACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGGAACCGTGGTGGTGTAgg                                                                         <  8:42652/149‑1 (MQ=255)
                                                                          aCCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGt                                                                        >  3:183328/1‑149 (MQ=255)
                                                                          aCCGACTCTACCAGCACGTACTGGCCGGTGCCCGGGTTG‑AGCTTCAGCAGCTCGATGGTGGTGCCGTTGACCAGGCTCAACAGGTTGGAGCAGTTGAACACC‑ACCTGCAGGTCGGCCGAGGTATTGGCGGCAACCGAGGTGGTGTAg                                                                          >  4:391321/1‑147 (MQ=255)
                                                                           ccGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTg                                                                       <  1:357868/149‑1 (MQ=255)
                                                                            cGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTgg                                                                      <  6:405750/149‑1 (MQ=255)
                                                                                tGTACCAGCACGTACTGGCCGGTGGCCGGGTTGTAGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCgtggt                                                                                 >  1:260369/1‑135 (MQ=255)
                                                                                tGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCgtggt                                                                                 <  2:260369/134‑1 (MQ=255)
                                                                                   aCCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCg                                                               >  5:329132/1‑149 (MQ=255)
                                                                                    ccAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCgg                                                              >  2:286826/1‑149 (MQ=255)
                                                                                     cAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCggg                                                             <  2:210350/149‑1 (MQ=255)
                                                                                      aGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGgtgt                                                                            >  5:153137/1‑133 (MQ=255)
                                                                                      aGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGgtgt                                                                            <  6:153137/133‑1 (MQ=255)
                                                                                       gCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTa                                                           >  5:204940/1‑149 (MQ=255)
                                                                                       gCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTa                                                           >  7:361886/1‑149 (MQ=255)
                                                                                              cTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCa                                                    <  8:238416/149‑1 (MQ=255)
                                                                                                    ggtggccggGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCa                                              <  3:391321/149‑1 (MQ=255)
                                                                                                      tggccggGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAgg                                            <  5:63807/149‑1 (MQ=255)
                                                                                                      tggccggGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCAGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAgg                                            <  7:341309/149‑1 (MQ=255)
                                                                                                        gccggGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCTGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGtt                                          <  3:102767/149‑1 (MQ=255)
                                                                                                        gccggGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGtt                                          <  4:183328/149‑1 (MQ=255)
                                                                                                           gggTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAg                                       <  5:239611/149‑1 (MQ=255)
                                                                                                                g‑agcttgagcAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAg                                   <  4:1467/148‑1 (MQ=255)
                                                                                                                  agcttgagcAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGc                                  <  5:103860/148‑1 (MQ=255)
                                                                                                                             cTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCGTActgc                      >  5:180863/1‑149 (MQ=255)
                                                                                                                                gATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACCGACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCGTActgctg                    >  1:299533/1‑149 (MQ=255)
                                                                                                                                gATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGTCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCGTActgctg                    <  7:375887/148‑1 (MQ=255)
                                                                                                                                 aTGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCATActgctgct                  >  7:78471/1‑149 (MQ=255)
                                                                                                                                  tggtggTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCGTActgctgctg                 <  6:152285/149‑1 (MQ=255)
                                                                                                                                   ggtggtGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCGTActgctgctg                 <  4:103112/148‑1 (MQ=255)
                                                                                                                                    gtggtgCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCGTACTGCTGCTGGa               <  8:308830/149‑1 (MQ=255)
                                                                                                                                    gtggtgCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCGTACTGCTGCTGGa               <  8:361886/149‑1 (MQ=255)
                                                                                                                                        acctgTTGAGCCTGCTCAACGGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGc                                                                     >  5:418965/5‑91 (MQ=255)
                                                                                                                                        acctgTTGAGCCTGCTCAACGGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGc                                                                     <  6:418965/87‑1 (MQ=255)
                                                                                                                                         cctgTTGAGCCTGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCGTACTGCTGCTGGAGTCCa          >  1:3998/4‑149 (MQ=255)
                                                                                                                                            gTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCGTACTGCTGCTGGAGTCCAcgc       >  1:45073/1‑149 (MQ=255)
                                                                                                                                               gAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACCGGCAGGTTCAGCGAGCCCAGCGTACTGCTGCTGGAGTCCACGCGGt    >  6:184780/1‑149 (MQ=255)
                                                                                                                                                 gCAGTCTCAACAGGTTGTTTGAGTTGACAACC‑ACCTGCAGGTCTGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCGTACTGCTGCTGGAGTCCACGCGGtt   <  8:350759/148‑1 (MQ=255)
                                                                                                                                                 gCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCGTACTGCTGCTGGAGTCCACGCGGTTg  >  4:396276/1‑149 (MQ=255)
                                                                                                                                                 gCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCGTACTGCTGCTGGAGTCCACGCGGTTg  <  3:75246/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGGTACCGGCGCCCTGGTTCTCGAAGGTATACCCGGCGCCGCCGCCCAGTATGCTGACCAGCGAACCACCTGGCACCGACTGTACCAGCACGTACTGGCCGGTGGCCGGGTTG‑AGCTTGAGCAGCTCGATGGTGGTGCCGTTGAGCAGGCTCAACAGGTTGGTGGAGTTGACCACC‑ACCTGCAGGTCGGCCGTGGTATTGGCGGCAACCGTGGTGGTGTAGGTGGCCTGGCGGGTACCGATCACTGGCAGGTTCAGCGAGCCCAGCGTACTGCTGCTGGAGTCCACGCGGTTG  >  NC_002947/927624‑927916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑