Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	92,184	G→T	7.8%	L66M (CTG→ATG)	trpA ←	tryptophan synthase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	92,184	0	G	T	7.8%	202.2 / 6.5	64	L66M (CTG→ATG)	trpA	tryptophan synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (34/25);  new base T (2/3);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

AACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGT‑CGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGA  >  NC_002947/92039‑92331                                                                                                                                                  |                                                                                                                                                     aaCGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGACCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTg                                                                                                                                                   >  2:374151/1‑149 (MQ=255)    gtgtCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGAt                                                                                                                                                >  3:390778/1‑149 (MQ=255)    gtgtCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGAt                                                                                                                                                <  8:332797/149‑1 (MQ=255)          aCGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGc                                                                                                                                                     <  6:388441/138‑1 (MQ=255)          aCGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGc                                                                                                                                                     >  5:388441/1‑138 (MQ=255)             ccAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGCGTGGCCATCCAGCTGGCCAccct                                                                                                                                       <  5:187682/149‑4 (MQ=255)                        gATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGTCATCCAGCTGGCCAccctgc                                                                                                                                     <  4:173610/140‑6 (MQ=255)                        gATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGTCATCCAGCTGGCCAccctgc                                                                                                                                     >  3:173610/1‑135 (MQ=255)                        gATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgccatcggccatcg                                                                                                                            >  2:327285/1‑149 (MQ=255)                             ggTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCg                                                                                                                       >  4:394024/1‑149 (MQ=255)                                   agtagCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAAc                                                                                                                 >  4:110851/1‑149 (MQ=255)                                    gtagCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgccatcggccatc                                                                                                                             <  8:278391/136‑1 (MQ=255)                                    gtagCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgccatcggccatc                                                                                                                             >  7:278391/1‑136 (MQ=255)                                       gCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGc                                                                                                              <  4:390778/148‑1 (MQ=255)                                        cccATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCAt                                                                                                            >  3:15064/1‑149 (MQ=255)                                          cATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGc                                                                                                          >  2:175883/1‑149 (MQ=255)                                          cATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACCCCATCGGCCATCGGGTCGGTGAACGGCAtcc                                                                                                          >  7:73528/1‑147 (MQ=255)                                             cAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGa                                                                                                                                                 <  5:84639/107‑1 (MQ=255)                                             cAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGa                                                                                                                                                 >  6:84639/1‑107 (MQ=255)                                                cACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCt                                                                                                    >  8:71125/1‑149 (MQ=255)                                                 aCGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTc                                                                                                   <  1:327285/149‑1 (MQ=255)                                                    aTCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGAt                                                                                                <  1:175883/149‑1 (MQ=255)                                                       ggCGTGGTCTGGTTATCGAGACGTAATTGGCGTACGATCTGCAGTGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCa                                                                                              <  8:73528/148‑1 (MQ=255)                                                        gCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGGATGCCCAGCTCGATCACg                                                                                            >  7:177019/1‑149 (MQ=255)                                                         cGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGt                                                                                           <  8:8388/149‑1 (MQ=255)                                                              tCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGgcg                                                                                      >  2:271421/1‑149 (MQ=255)                                                                  gTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCcag                                                                                  >  2:274181/1‑149 (MQ=255)                                                                  gTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCcag                                                                                  >  5:230757/1‑149 (MQ=255)                                                                  gTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGATTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCcag                                                                                  >  6:306085/1‑149 (MQ=255)                                                                        cacaCGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCg                                                                            >  1:254252/1‑149 (MQ=255)                                                                        cacaCGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCg                                                                            >  2:15846/1‑149 (MQ=255)                                                                        cacaCGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCg                                                                            <  4:15064/149‑1 (MQ=255)                                                                           acGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCa                                                                         <  1:15846/149‑1 (MQ=255)                                                                            cGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAg                                                                        <  3:290324/149‑1 (MQ=255)                                                                            cGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGGGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAg                                                                        <  7:305452/149‑1 (MQ=255)                                                                              gAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGc                                                                      <  3:110851/149‑1 (MQ=255)                                                                                 ttCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCt                                                                   >  2:43096/1‑149 (MQ=255)                                                                                    gcgAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCtt                                                                  >  5:218644/1‑147 (MQ=255)                                                                                     cgAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAg                                                               <  2:254252/149‑1 (MQ=255)                                                                                      gAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAgg                                                              <  3:394024/149‑1 (MQ=255)                                                                                          cATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCt                                                          <  6:230757/149‑1 (MQ=255)                                                                                              tGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCaa                                                      >  6:418246/1‑149 (MQ=255)                                                                                               gCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAAc                                                     >  8:19932/1‑149 (MQ=255)                                                                                                  gggTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAg                                                  >  4:174851/1‑149 (MQ=255)                                                                                                     tCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcg                                               >  7:309401/1‑149 (MQ=255)                                                                                                     tCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcg                                               >  7:267424/1‑149 (MQ=255)                                                                                                      cTTGGCCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcgt                                              >  1:274465/1‑149 (MQ=255)                                                                                                          aCCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcgt‑cg                                           >  2:254661/1‑147 (MQ=255)                                                                                                            cAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGT‑CGTAGc                                       >  8:215458/1‑149 (MQ=255)                                                                                                              aGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCAATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGT‑CGTAGccc                                     >  1:95290/1‑149 (MQ=255)                                                                                                                  cTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGT‑CGTAGCCCGGGt                                 <  7:19932/149‑1 (MQ=255)                                                                                                                   tGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGT‑CGTAGCCCGGGTc                                >  6:194640/1‑149 (MQ=255)                                                                                                                     gCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGT‑CGTAGCCCGGGTCGc                              >  3:355691/1‑149 (MQ=255)                                                                                                                       cAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGT‑CGTAGCCCGGGTCGc                              >  6:408984/1‑147 (MQ=255)                                                                                                                       cAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGT‑CGTAGCCCGGGTCGCCa                            <  2:274465/149‑1 (MQ=255)                                                                                                                         gCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGG‑CGTAGCCCGGGTCGCCAGc                          <  5:418246/149‑1 (MQ=255)                                                                                                                                 ggCGCGCAGGGTGGCCAGCTGGATGGACACTCCATAGTCCATCGGGTCGGTGAACGGAATGCCCAGCTCGATCAAGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATATGCAACGAGGCGT‑CGTAGCCAGGGTCGCCAGCTGTGACg                   >  4:234301/1‑148 (MQ=255)                                                                                                                                   cgcgcAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGT‑CGTAGCCCGGGTCGCCAGCTGTGACGAAg                <  7:71125/149‑1 (MQ=255)                                                                                                                                   cgcgcAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGT‑CGTAGCCCGGGTCGCCAGCTGTGACGAAg                <  2:95290/149‑1 (MQ=255)                                                                                                                                     cgcAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGT‑CGTAGCCCGGGTCGCCAGCTGTGACGAAGGt              <  1:374151/149‑1 (MQ=255)                                                                                                                                      gcAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCCGTAGCCCGGGTCGCCAGCTGTGACGAAGGt              <  1:43096/149‑1 (MQ=255)                                                                                                                                       tggcgTGGCCATCCAGCTGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCt                                                          <  3:349896/100‑1 (MQ=255)                                                                                                                                       tggcgTGGCCATCCAGCTGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCt                                                          >  4:349896/5‑104 (MQ=255)                                                                                                                                                 aGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGT‑CGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGa  <  6:185165/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                     AACGTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGT‑CGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGA  >  NC_002947/92039‑92331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑