Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 932,435 G→A 6.4% A4396V (GCC→GTC)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947932,4350GA 6.4% 237.1 / 3.9 78A4396V (GCC→GTC) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (32/41);  new base A (3/2);  total (35/43)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

GTTGTCCGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGG  >  NC_002947/932290‑932582
                                                                                                                                                 |                                                                                                                                                   
gTTGTCCGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTcggcgg                                                                                                                                                  >  5:221863/1‑149 (MQ=255)
 ttGTCCGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCggcggc                                                                                                                                                 >  8:215276/1‑149 (MQ=255)
   gTCCGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGcc                                                                                                                                                <  1:407810/148‑1 (MQ=255)
   gTCCGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCa                                                                                                                                               >  3:304367/1‑149 (MQ=255)
    tCCGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCAc                                                                                                                                              >  1:49823/1‑149 (MQ=255)
     ccGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACt                                                                                                                                             <  7:165535/149‑1 (MQ=255)
      cGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTg                                                                                                                                            >  3:227969/1‑149 (MQ=255)
         cGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAg                                                                                                                                         <  8:309985/149‑1 (MQ=255)
               cGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGc                                                                                                                                   <  5:384480/149‑1 (MQ=255)
               cGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTACAGTGGCCGCCGACGGTCACTTCAGCGtg                                                                                                                                     <  1:355998/147‑2 (MQ=38)
               cGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTACAGTGGCCGCCGACGGTCACTTCAGCGtg                                                                                                                                     >  2:355998/1‑146 (MQ=38)
               cGGTGCCAGCGCGCCCGGCGCCGTCACGGTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGtt                                                                                                                                     <  7:116557/147‑1 (MQ=255)
               cGGTGCCAGCGCGCCCGGCGCCGTCACGGTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGtt                                                                                                                                     >  8:116557/1‑147 (MQ=255)
                ggTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGATAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGcc                                                                                                                                  <  1:84223/149‑1 (MQ=255)
                ggTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGcc                                                                                                                                  <  4:312937/149‑1 (MQ=255)
                   gCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAg                                                                                                                               <  4:304367/149‑1 (MQ=255)
                       gcgcgcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACg                                                                                                                           <  7:116441/149‑1 (MQ=255)
                        cgcgcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACgg                                                                                                                          >  1:177291/1‑149 (MQ=255)
                         gcgcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGt                                                                                                                         <  7:298278/149‑1 (MQ=255)
                           gcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCGCTGTAGCGTTGCCCAGCCAGGTAc                                                                                                                       >  4:310297/1‑149 (MQ=255)
                             ccGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTACAGTGGCCGCCGACGGTCACTTCAGCGtgtcc                                                                                                                                  <  2:46130/136‑5 (MQ=37)
                             ccGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTACAGTGGCCGCCGACGGTCACTTCAGCGtgtcc                                                                                                                                  >  1:46130/1‑132 (MQ=37)
                                   ccGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGt                                                                                                               <  2:49823/149‑1 (MQ=255)
                                    cGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTg                                                                                                              <  8:343794/149‑1 (MQ=255)
                                      tCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTc                                                                                                                   <  8:66310/142‑1 (MQ=255)
                                      tCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTc                                                                                                                   >  7:66310/1‑142 (MQ=255)
                                      tCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCt                                                                                                            <  3:394166/149‑1 (MQ=255)
                                                cgcAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCa                                                                                                  <  3:121861/149‑1 (MQ=255)
                                                  cAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACg                                                                                                <  2:177291/149‑1 (MQ=255)
                                                     gggAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCTTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGtt                                                                                             <  2:286466/149‑1 (MQ=255)
                                                        agaCATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTgcg                                                                                          >  3:115047/1‑149 (MQ=255)
                                                             ttCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACt                                                                                                                                             <  6:310581/93‑1 (MQ=255)
                                                             ttCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACt                                                                                                                                             >  5:310581/1‑93 (MQ=255)
                                                                  tgCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTc                                                                                  <  7:215276/147‑1 (MQ=255)
                                                                  tgCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCg                                                                                 <  3:310297/148‑1 (MQ=255)
                                                                     ggCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCAt                                                                             <  8:44305/149‑1 (MQ=255)
                                                                      gCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATc                                                                            <  4:77619/149‑1 (MQ=255)
                                                                          cGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCt                                                                        <  6:221863/149‑1 (MQ=255)
                                                                             ccTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGc                                                                                                                                   >  8:64401/1‑87 (MQ=255)
                                                                             ccTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGc                                                                                                                                   <  7:64401/87‑1 (MQ=255)
                                                                                 gACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAgg                                                                  <  5:294925/148‑1 (MQ=255)
                                                                                   ccACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTg                                                               >  1:172530/1‑149 (MQ=255)
                                                                                    cACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTgg                                                              >  5:233426/1‑149 (MQ=255)
                                                                                    cACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTgg                                                              >  4:95129/1‑149 (MQ=255)
                                                                                         tCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCa                                                         >  7:314352/1‑149 (MQ=255)
                                                                                           aGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCAtt                                                       <  7:155127/149‑1 (MQ=255)
                                                                                           aGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCAtt                                                       >  6:347713/1‑149 (MQ=255)
                                                                                                 tCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCg                                                   >  3:56672/1‑147 (MQ=255)
                                                                                                   aCCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGGCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                               <  5:397252/149‑1 (MQ=255)
                                                                                                    cccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGAc                                              <  7:178970/149‑1 (MQ=255)
                                                                                                     ccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                               >  8:288845/1‑147 (MQ=255)
                                                                                                     ccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                               >  6:269248/1‑147 (MQ=255)
                                                                                                     ccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                               >  6:389626/1‑147 (MQ=255)
                                                                                                     ccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACa                                             <  7:288845/149‑1 (MQ=255)
                                                                                                          aTCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAg                                        >  8:41581/1‑149 (MQ=255)
                                                                                                          aTCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAg                                        <  3:95129/149‑1 (MQ=255)
                                                                                                           tCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAgg                                       <  5:389626/149‑1 (MQ=255)
                                                                                                             gCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGtt                                     <  6:5896/149‑1 (MQ=255)
                                                                                                              cTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTg                                    <  6:320282/149‑1 (MQ=255)
                                                                                                              cTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTg                                    >  4:73002/1‑149 (MQ=255)
                                                                                                                 ccGGTGACAGGGACACGCTGAAGTGACCGTGGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGc                                  >  1:33850/1‑148 (MQ=255)
                                                                                                                 ccGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGc                                  <  1:178062/148‑1 (MQ=255)
                                                                                                                 ccGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCGAGTACCGTCACGGTTGCGCCTGCGTCGCAATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGCTTGGcc                                 >  3:226049/1‑149 (MQ=255)
                                                                                                                        cAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcgg                          <  4:115047/149‑1 (MQ=255)
                                                                                                                           ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGt                                                                                                         <  8:118065/67‑1 (MQ=255)
                                                                                                                           ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGt                                                                                                         >  7:118065/1‑67 (MQ=255)
                                                                                                                           ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGtt                                     <  1:286825/135‑1 (MQ=255)
                                                                                                                           ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGtt                                     >  2:286825/1‑135 (MQ=255)
                                                                                                                           ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcggccg                       <  4:227969/149‑1 (MQ=255)
                                                                                                                             acacGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcggccggc                     >  7:9183/1‑149 (MQ=255)
                                                                                                                                cGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcggccggcggc                  <  7:41581/149‑1 (MQ=255)
                                                                                                                                   tacaGTGGCCGCCGACGGTCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCgtg               >  8:295198/4‑149 (MQ=255)
                                                                                                                                     aaGTGACCGGCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCgtggt             <  8:295850/149‑1 (MQ=255)
                                                                                                                                       gTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGgtgt           >  8:263270/1‑149 (MQ=255)
                                                                                                                                         gACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTcc         >  7:231216/1‑149 (MQ=255)
                                                                                                                                         gACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTc          >  4:302545/1‑148 (MQ=255)
                                                                                                                                         gACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTc          <  3:302545/148‑1 (MQ=255)
                                                                                                                                           ccGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGc       >  6:20108/1‑149 (MQ=255)
                                                                                                                                             gTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTg     >  6:290434/1‑149 (MQ=255)
                                                                                                                                              tCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGc    >  1:289317/1‑149 (MQ=255)
                                                                                                                                                ggcggcCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCgg  <  6:233426/149‑1 (MQ=255)
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GTTGTCCGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGG  >  NC_002947/932290‑932582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑