Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 932,439 C→T 6.5% A4395T (GCC→ACC)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947932,4390CT 6.5% 235.1 / 3.9 77A4395T (GCC→ACC) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (32/40);  new base T (3/2);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

TTGTCCGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGG  >  NC_002947/932291‑932582
                                                                                                                                                    |                                                                                                                                               
ttGTCCGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCggcggc                                                                                                                                                 >  8:215276/1‑149 (MQ=255)
  gTCCGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGcc                                                                                                                                                <  1:407810/148‑1 (MQ=255)
  gTCCGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCa                                                                                                                                               >  3:304367/1‑149 (MQ=255)
   tCCGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCAc                                                                                                                                              >  1:49823/1‑149 (MQ=255)
    ccGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACt                                                                                                                                             <  7:165535/149‑1 (MQ=255)
     cGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTg                                                                                                                                            >  3:227969/1‑149 (MQ=255)
        cGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAg                                                                                                                                         <  8:309985/149‑1 (MQ=255)
              cGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGc                                                                                                                                   <  5:384480/149‑1 (MQ=255)
              cGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTACAGTGGCCGCCGACGGTCACTTCAGCGtg                                                                                                                                     <  1:355998/147‑2 (MQ=38)
              cGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTACAGTGGCCGCCGACGGTCACTTCAGCGtg                                                                                                                                     >  2:355998/1‑146 (MQ=38)
              cGGTGCCAGCGCGCCCGGCGCCGTCACGGTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGtt                                                                                                                                     <  7:116557/147‑1 (MQ=255)
              cGGTGCCAGCGCGCCCGGCGCCGTCACGGTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGtt                                                                                                                                     >  8:116557/1‑147 (MQ=255)
               ggTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGATAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGcc                                                                                                                                  <  1:84223/149‑1 (MQ=255)
               ggTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGcc                                                                                                                                  <  4:312937/149‑1 (MQ=255)
                  gCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAg                                                                                                                               <  4:304367/149‑1 (MQ=255)
                      gcgcgcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACg                                                                                                                           <  7:116441/149‑1 (MQ=255)
                       cgcgcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACgg                                                                                                                          >  1:177291/1‑149 (MQ=255)
                        gcgcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGt                                                                                                                         <  7:298278/149‑1 (MQ=255)
                          gcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCGCTGTAGCGTTGCCCAGCCAGGTAc                                                                                                                       >  4:310297/1‑149 (MQ=255)
                            ccGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTACAGTGGCCGCCGACGGTCACTTCAGCGtgtcc                                                                                                                                  <  2:46130/136‑5 (MQ=37)
                            ccGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTACAGTGGCCGCCGACGGTCACTTCAGCGtgtcc                                                                                                                                  >  1:46130/1‑132 (MQ=37)
                                  ccGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGt                                                                                                               <  2:49823/149‑1 (MQ=255)
                                   cGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTg                                                                                                              <  8:343794/149‑1 (MQ=255)
                                     tCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTc                                                                                                                   >  7:66310/1‑142 (MQ=255)
                                     tCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTc                                                                                                                   <  8:66310/142‑1 (MQ=255)
                                     tCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCt                                                                                                            <  3:394166/149‑1 (MQ=255)
                                               cgcAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCa                                                                                                  <  3:121861/149‑1 (MQ=255)
                                                 cAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACg                                                                                                <  2:177291/149‑1 (MQ=255)
                                                    gggAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCTTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGtt                                                                                             <  2:286466/149‑1 (MQ=255)
                                                       agaCATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTgcg                                                                                          >  3:115047/1‑149 (MQ=255)
                                                            ttCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACt                                                                                                                                             >  5:310581/1‑93 (MQ=255)
                                                            ttCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACt                                                                                                                                             <  6:310581/93‑1 (MQ=255)
                                                                 tgCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTc                                                                                  <  7:215276/147‑1 (MQ=255)
                                                                 tgCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCg                                                                                 <  3:310297/148‑1 (MQ=255)
                                                                    ggCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCAt                                                                             <  8:44305/149‑1 (MQ=255)
                                                                     gCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATc                                                                            <  4:77619/149‑1 (MQ=255)
                                                                         cGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCt                                                                        <  6:221863/149‑1 (MQ=255)
                                                                            ccTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGc                                                                                                                                   >  8:64401/1‑87 (MQ=255)
                                                                            ccTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGc                                                                                                                                   <  7:64401/87‑1 (MQ=255)
                                                                                gACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAgg                                                                  <  5:294925/148‑1 (MQ=255)
                                                                                  ccACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTg                                                               >  1:172530/1‑149 (MQ=255)
                                                                                   cACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTgg                                                              >  4:95129/1‑149 (MQ=255)
                                                                                   cACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTgg                                                              >  5:233426/1‑149 (MQ=255)
                                                                                        tCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCa                                                         >  7:314352/1‑149 (MQ=255)
                                                                                          aGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCAtt                                                       <  7:155127/149‑1 (MQ=255)
                                                                                          aGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCAtt                                                       >  6:347713/1‑149 (MQ=255)
                                                                                                tCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCg                                                   >  3:56672/1‑147 (MQ=255)
                                                                                                  aCCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGGCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                               <  5:397252/149‑1 (MQ=255)
                                                                                                   cccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGAc                                              <  7:178970/149‑1 (MQ=255)
                                                                                                    ccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                               >  8:288845/1‑147 (MQ=255)
                                                                                                    ccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                               >  6:269248/1‑147 (MQ=255)
                                                                                                    ccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                               >  6:389626/1‑147 (MQ=255)
                                                                                                    ccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACa                                             <  7:288845/149‑1 (MQ=255)
                                                                                                         aTCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAg                                        <  3:95129/149‑1 (MQ=255)
                                                                                                         aTCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAg                                        >  8:41581/1‑149 (MQ=255)
                                                                                                          tCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAgg                                       <  5:389626/149‑1 (MQ=255)
                                                                                                            gCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGtt                                     <  6:5896/149‑1 (MQ=255)
                                                                                                             cTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTg                                    <  6:320282/149‑1 (MQ=255)
                                                                                                             cTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTg                                    >  4:73002/1‑149 (MQ=255)
                                                                                                                ccGGTGACAGGGACACGCTGAAGTGACCGTGGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGc                                  >  1:33850/1‑148 (MQ=255)
                                                                                                                ccGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGc                                  <  1:178062/148‑1 (MQ=255)
                                                                                                                ccGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCGAGTACCGTCACGGTTGCGCCTGCGTCGCAATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGCTTGGcc                                 >  3:226049/1‑149 (MQ=255)
                                                                                                                       cAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcgg                          <  4:115047/149‑1 (MQ=255)
                                                                                                                          ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGt                                                                                                         >  7:118065/1‑67 (MQ=255)
                                                                                                                          ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGt                                                                                                         <  8:118065/67‑1 (MQ=255)
                                                                                                                          ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGtt                                     >  2:286825/1‑135 (MQ=255)
                                                                                                                          ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGtt                                     <  1:286825/135‑1 (MQ=255)
                                                                                                                          ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcggccg                       <  4:227969/149‑1 (MQ=255)
                                                                                                                            acacGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcggccggc                     >  7:9183/1‑149 (MQ=255)
                                                                                                                               cGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcggccggcggc                  <  7:41581/149‑1 (MQ=255)
                                                                                                                                  tacaGTGGCCGCCGACGGTCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCgtg               >  8:295198/4‑149 (MQ=255)
                                                                                                                                    aaGTGACCGGCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCgtggt             <  8:295850/149‑1 (MQ=255)
                                                                                                                                      gTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGgtgt           >  8:263270/1‑149 (MQ=255)
                                                                                                                                        gACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTcc         >  7:231216/1‑149 (MQ=255)
                                                                                                                                        gACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTc          >  4:302545/1‑148 (MQ=255)
                                                                                                                                        gACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTc          <  3:302545/148‑1 (MQ=255)
                                                                                                                                          ccGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGc       >  6:20108/1‑149 (MQ=255)
                                                                                                                                            gTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTg     >  6:290434/1‑149 (MQ=255)
                                                                                                                                             tCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGc    >  1:289317/1‑149 (MQ=255)
                                                                                                                                               ggcggcCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCgg  <  6:233426/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TTGTCCGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGG  >  NC_002947/932291‑932582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑