Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	932,444	G→T	5.5%	T4393K (ACA→AAA)	PP_0806 ←	surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	932,444	0	G	T	5.5%	244.1 / 3.3	73	T4393K (ACA→AAA)	PP_0806	surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (30/39);  new base T (2/2);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTG  >  NC_002947/932296‑932591                                                                                                                                                     |                                                                                                                                                    cGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTg                                                                                                                                                     >  3:227969/1‑149 (MQ=255)    cGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAg                                                                                                                                                  <  8:309985/149‑1 (MQ=255)          cGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGc                                                                                                                                            <  5:384480/149‑1 (MQ=255)          cGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTACAGTGGCCGCCGACGGTCACTTCAGCGtg                                                                                                                                              <  1:355998/147‑2 (MQ=38)          cGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTACAGTGGCCGCCGACGGTCACTTCAGCGtg                                                                                                                                              >  2:355998/1‑146 (MQ=38)          cGGTGCCAGCGCGCCCGGCGCCGTCACGGTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGtt                                                                                                                                              >  8:116557/1‑147 (MQ=255)          cGGTGCCAGCGCGCCCGGCGCCGTCACGGTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGtt                                                                                                                                              <  7:116557/147‑1 (MQ=255)           ggTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGATAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGcc                                                                                                                                           <  1:84223/149‑1 (MQ=255)           ggTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGcc                                                                                                                                           <  4:312937/149‑1 (MQ=255)              gCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAg                                                                                                                                        <  4:304367/149‑1 (MQ=255)                  gcgcgcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACg                                                                                                                                    <  7:116441/149‑1 (MQ=255)                   cgcgcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACgg                                                                                                                                   >  1:177291/1‑149 (MQ=255)                    gcgcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGt                                                                                                                                  <  7:298278/149‑1 (MQ=255)                      gcCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCGCTGTAGCGTTGCCCAGCCAGGTAc                                                                                                                                >  4:310297/1‑149 (MQ=255)                        ccGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTACAGTGGCCGCCGACGGTCACTTCAGCGtgtcc                                                                                                                                           >  1:46130/1‑132 (MQ=37)                        ccGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTACAGTGGCCGCCGACGGTCACTTCAGCGtgtcc                                                                                                                                           <  2:46130/136‑5 (MQ=37)                              ccGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGt                                                                                                                        <  2:49823/149‑1 (MQ=255)                               cGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTg                                                                                                                       <  8:343794/149‑1 (MQ=255)                                 tCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTc                                                                                                                            >  7:66310/1‑142 (MQ=255)                                 tCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTc                                                                                                                            <  8:66310/142‑1 (MQ=255)                                 tCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCt                                                                                                                     <  3:394166/149‑1 (MQ=255)                                           cgcAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCa                                                                                                           <  3:121861/149‑1 (MQ=255)                                             cAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACg                                                                                                         <  2:177291/149‑1 (MQ=255)                                                gggAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCTTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGtt                                                                                                      <  2:286466/149‑1 (MQ=255)                                                   agaCATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTgcg                                                                                                   >  3:115047/1‑149 (MQ=255)                                                             tgCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTc                                                                                           <  7:215276/147‑1 (MQ=255)                                                             tgCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCg                                                                                          <  3:310297/148‑1 (MQ=255)                                                                ggCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCAt                                                                                      <  8:44305/149‑1 (MQ=255)                                                                 gCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATc                                                                                     <  4:77619/149‑1 (MQ=255)                                                                     cGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCt                                                                                 <  6:221863/149‑1 (MQ=255)                                                                        ccTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGc                                                                                                                                            >  8:64401/1‑87 (MQ=255)                                                                        ccTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGc                                                                                                                                            <  7:64401/87‑1 (MQ=255)                                                                            gACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAgg                                                                           <  5:294925/148‑1 (MQ=255)                                                                              ccACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTg                                                                        >  1:172530/1‑149 (MQ=255)                                                                               cACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTgg                                                                       >  4:95129/1‑149 (MQ=255)                                                                               cACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTgg                                                                       >  5:233426/1‑149 (MQ=255)                                                                                    tCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCa                                                                  >  7:314352/1‑149 (MQ=255)                                                                                      aGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCAtt                                                                <  7:155127/149‑1 (MQ=255)                                                                                      aGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCAtt                                                                >  6:347713/1‑149 (MQ=255)                                                                                            tCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCg                                                            >  3:56672/1‑147 (MQ=255)                                                                                              aCCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGGCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                                        <  5:397252/149‑1 (MQ=255)                                                                                               cccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGAc                                                       <  7:178970/149‑1 (MQ=255)                                                                                                ccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                                        >  6:269248/1‑147 (MQ=255)                                                                                                ccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                                        >  8:288845/1‑147 (MQ=255)                                                                                                ccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGgaga                                                        >  6:389626/1‑147 (MQ=255)                                                                                                ccGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACa                                                      <  7:288845/149‑1 (MQ=255)                                                                                                     aTCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAg                                                 <  3:95129/149‑1 (MQ=255)                                                                                                     aTCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAg                                                 >  8:41581/1‑149 (MQ=255)                                                                                                      tCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAgg                                                <  5:389626/149‑1 (MQ=255)                                                                                                        gCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGtt                                              <  6:5896/149‑1 (MQ=255)                                                                                                         cTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTg                                             >  4:73002/1‑149 (MQ=255)                                                                                                         cTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTg                                             <  6:320282/149‑1 (MQ=255)                                                                                                            ccGGTGACAGGGACACGCTGAAGTGACCGTGGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGc                                           >  1:33850/1‑148 (MQ=255)                                                                                                            ccGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGc                                           <  1:178062/148‑1 (MQ=255)                                                                                                            ccGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCGAGTACCGTCACGGTTGCGCCTGCGTCGCAATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGCTTGGcc                                          >  3:226049/1‑149 (MQ=255)                                                                                                                   cAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcgg                                   <  4:115047/149‑1 (MQ=255)                                                                                                                      ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGt                                                                                                                  >  7:118065/1‑67 (MQ=255)                                                                                                                      ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGt                                                                                                                  <  8:118065/67‑1 (MQ=255)                                                                                                                      ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGtt                                              <  1:286825/135‑1 (MQ=255)                                                                                                                      ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGtt                                              >  2:286825/1‑135 (MQ=255)                                                                                                                      ggACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcggccg                                <  4:227969/149‑1 (MQ=255)                                                                                                                        acacGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcggccggc                              >  7:9183/1‑149 (MQ=255)                                                                                                                           cGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCAcggcggccggcggc                           <  7:41581/149‑1 (MQ=255)                                                                                                                              tacaGTGGCCGCCGACGGTCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCgtg                        >  8:295198/4‑149 (MQ=255)                                                                                                                                aaGTGACCGGCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCgtggt                      <  8:295850/149‑1 (MQ=255)                                                                                                                                  gTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGgtgt                    >  8:263270/1‑149 (MQ=255)                                                                                                                                    gACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTcc                  >  7:231216/1‑149 (MQ=255)                                                                                                                                    gACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTc                   >  4:302545/1‑148 (MQ=255)                                                                                                                                    gACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTc                   <  3:302545/148‑1 (MQ=255)                                                                                                                                      ccGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGc                >  6:20108/1‑149 (MQ=255)                                                                                                                                        gTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTg              >  6:290434/1‑149 (MQ=255)                                                                                                                                         tCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGc             >  1:289317/1‑149 (MQ=255)                                                                                                                                           ggcggcCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCgg           <  6:233426/149‑1 (MQ=255)                                                                                                                                                    tGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTg  >  5:401131/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CGGCGTGGCCGGTGCCAGCGCGCCCGGCGCCGTCACGTTTTGCGCAGGGGAGACATTCTGTGCGGCATCGGCCTGGACCACGCTCAGGCTTTCACCCGTGATCGCTGCCGGTGACAGGGACACGCTGAAGTGACCGTCGGCGGCCACTGTAGCGTTGCCCAGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTG  >  NC_002947/932296‑932591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑