Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,013,286 C→T 9.5% intergenic (+114/+17) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,013,2860CT 9.5% 110.5 / 2.8 42intergenic (+114/+17)prfC/potF‑Ipeptide chain release factor 3/putrescine‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (25/13);  new base T (2/2);  total (27/15)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

ACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCTCGTCGCTCGGGTAAATCATCGTATTACCCTTCATCG  >  NC_002947/1013211‑1013433
                                                                           |                                                                                                                                                    
aCGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCttt                                                                                                                      <  5:35886/107‑1 (MQ=255)
aCGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCttt                                                                                                                      >  6:35886/1‑107 (MQ=255)
    ccGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCgg                                                                         >  5:143606/1‑148 (MQ=21)
    ccGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCgg                                                                         >  2:387835/1‑148 (MQ=255)
           cacaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTc                                                                                                     <  5:307064/113‑1 (MQ=255)
           cacaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTc                                                                                                     >  6:307064/1‑113 (MQ=255)
            acaGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                                                                >  3:144859/1‑149 (MQ=21)
            acaGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                                                                >  3:150699/1‑149 (MQ=21)
            acaGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGt                                                                                                             <  3:259562/104‑1 (MQ=14)
            acaGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGt                                                                                                             >  4:259562/1‑104 (MQ=14)
            acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                                                                >  3:19214/1‑149 (MQ=255)
                gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgc                                                            >  2:15707/1‑149 (MQ=255)
                 aaTCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgc                                                            >  5:365350/1‑148 (MQ=255)
                       cAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATa                                                       >  3:313513/1‑147 (MQ=255)
                            cTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGc                                                                            <  1:160593/121‑1 (MQ=255)
                            cTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTg                                                >  5:260709/1‑149 (MQ=255)
                               gAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGcc                                              <  1:387835/148‑1 (MQ=255)
                               caGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGc                                               <  6:143606/146‑1 (MQ=255)
                                  gTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCAt                                           <  4:313513/148‑1 (MQ=255)
                                     aTGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCCCATACAGCTAGCCCATCAc                                        >  2:38204/1‑148 (MQ=255)
                                       gtgATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCtc                                     >  6:46423/1‑149 (MQ=255)
                                       gtgATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCtc                                     >  7:212503/1‑149 (MQ=255)
                                       gtgATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCtc                                     >  3:191788/1‑149 (MQ=255)
                                         gATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCg                                                                          <  5:140092/110‑1 (MQ=255)
                                         gATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCg                                                                          >  6:140092/1‑110 (MQ=255)
                                          attccTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAAGTTTCCGGCTTTGATACTGGTCCATATTCGCGTACGAAAACGGTCGATCTTCGCCGGCATCGCATCCAGCGCATACAGATTGCCCAACACAtcga                                   >  1:309767/4‑148 (MQ=38)
                                          aTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCtcgtc                                  >  5:133135/1‑149 (MQ=255)
                                              cTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCTCGTCGCTc                              >  4:138530/1‑149 (MQ=255)
                                               tgtgGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGTATCGCCTCCAgc                                                            <  8:284254/118‑1 (MQ=255)
                                               tgtgGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGTATCGCCTCCAgc                                                            >  7:284254/1‑118 (MQ=255)
                                                   ggAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCTCGTCGCTCGGGt                          >  5:385559/1‑148 (MQ=255)
                                                   ggAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCTCGTCGCTCGGGt                          >  3:163146/1‑148 (MQ=255)
                                                       cGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCTCGTCGCTCGGGTAAatc                     <  3:138530/149‑1 (MQ=255)
                                                          gCGTGCCCGCGAAGGGCTGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCTCGTCGCTCGGGTAAatcatc                  <  4:150699/149‑1 (MQ=255)
                                                          gCGTGCCCGCGAAGGGCTGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCTCGTCGCTCGGGTAAatcatc                  <  4:144859/149‑1 (MQ=255)
                                                          gCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTg                                                >  4:193229/1‑119 (MQ=255)
                                                          gCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTg                                                <  3:193229/119‑1 (MQ=255)
                                                             tGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCTCGTCGCTCGGGTAAATCATCGTa               <  6:260709/149‑1 (MQ=255)
                                                                ccGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCAAAGCCTCCAGCGCATACAGCTAGCCCATCACCTCGTCGCTCGGGTAAATCATCGTATTa            >  5:276189/1‑149 (MQ=255)
                                                                ccGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATAAGGTCGATCTTAGCCGGCATCGCCTCCAGCGCATACAGATTGCCCATCACCTCGTCGCTCGGGTAAATCATCGTATTa            >  1:124262/1‑149 (MQ=255)
                                                                     aaGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCAACAGCGCATACAGCTTGCCCATCACCTCGTCGCTCGGGTAAATCATCGTATTACCCt        >  8:359028/1‑148 (MQ=255)
                                                                      aGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCTCGTCGCTCGGGTAAATCATCGTATTACCCTTc      <  5:46423/149‑1 (MQ=255)
                                                                          ccGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCTCGTCGCTCGGGTAAATCATCGTATTACCCTTCATCg  >  5:40522/1‑149 (MQ=255)
                                                                          ccGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCTCGTCGCTCGGGTAAATCATCGTATTACCCTTCATCg  >  8:325382/1‑149 (MQ=255)
                                                                           |                                                                                                                                                    
ACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAA‑TTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCTCGTCGCTCGGGTAAATCATCGTATTACCCTTCATCG  >  NC_002947/1013211‑1013433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑