Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,047,736 G→A 6.0% S214S (TCC→TCT)  PP_0906 ← RND family multidrug transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,047,7360GA 6.0% 199.8 / 2.5 67S214S (TCC→TCT) PP_0906RND family multidrug transporter
Reads supporting (aligned to +/- strand):  ref base G (35/28);  new base A (2/2);  total (37/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

CCATCTCGACCCGCGCCACATCGCCCAGCAGCACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCGGCCATGCCCGGCAGAGTGGCC  >  NC_002947/1047588‑1047883
                                                                                                                                                    |                                                                                                                                                   
ccATCTCGACCCGCGCCACATCGCCCAGCAGCACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAgcggcg                                                                                                                                                     >  2:227268/1‑149 (MQ=255)
 cATCTCGACCCGCGCCACATCGCCCAGCAGCACGCGGCTGTCACCGCTAGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAgcggcg                                                                                                                                                     >  1:94616/1‑148 (MQ=255)
  aTCTCGACCCGCGCCACATCGCCCAGCAGCACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGcggcgg                                                                                                                                                    >  6:86607/1‑148 (MQ=255)
   tctcGACCAGCGCCACATCGCCCAGCAGCACGCGGCTGTCACCGATGGTCATGAGCGGCAGGGCGGCGAACGCCTCGGACCACTTCAGCTCGCAGCTGGCGTTGACGCTGGTGACCACGTATTCGCCATTCACCTAGCAAGCGGCGGa                                                                                                                                                   >  7:55207/1‑148 (MQ=255)
       gACCCGCGCCACATCGCCCAGCAGCACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGaaa                                                                                                                                                 <  5:195510/146‑1 (MQ=255)
       gACCCGCGCCACATCGCCCAGCAGCACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAg                                                                                                                                                <  5:232323/147‑1 (MQ=255)
          ccGCGCCACATCGCCCAGCAGCACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGt                                                                                                                                           >  3:136230/1‑149 (MQ=255)
           cgcgCCACATCGCCCAGCAGCACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGtt                                                                                                                                          <  8:21414/149‑1 (MQ=255)
            gcgcCACATCGCCCAGCAGCACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTg                                                                                                                                         >  4:384058/1‑149 (MQ=255)
               ccACATCGCCCAGCAGCACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGCCCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAg                                                                                                                                      >  4:300046/1‑149 (MQ=255)
                  caTCGCCCAGCAGCACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAgcgg                                                                                                                                   <  7:236565/149‑1 (MQ=255)
                   aTCGCCCAGCAGCACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAgcggc                                                                                                                                  >  2:319229/1‑149 (MQ=255)
                   aTCGCCCAGCAGCACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAgcggc                                                                                                                                  <  2:391437/149‑1 (MQ=255)
                        ccAGCAGCACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACg                                                                                                                             >  2:94221/1‑149 (MQ=255)
                          agcagcACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACgg                                                                                                                            >  8:22944/1‑148 (MQ=255)
                                  gcgGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCa                                                                                                                   <  1:319229/149‑1 (MQ=255)
                                  gcgGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCa                                                                                                                   >  6:323325/1‑149 (MQ=255)
                                     gtgacagccGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGt                                                                                                                >  5:255056/8‑149 (MQ=255)
                                     gCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGt                                                                                                                <  6:291722/149‑1 (MQ=255)
                                     gCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGt                                                                                                                <  7:279843/149‑1 (MQ=255)
                                        gTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCgg                                                                                                             >  2:252566/1‑149 (MQ=255)
                                         tCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCgg                                                                                                             >  2:200318/1‑148 (MQ=255)
                                           aCCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAg                                                                                                          >  4:282119/1‑149 (MQ=255)
                                            ccGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACTGCAtt                                                                                                                        <  5:55721/134‑1 (MQ=255)
                                            ccGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCAtt                                                                                                                        >  6:55721/1‑134 (MQ=255)
                                            ccGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCACGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGc                                                                                                         >  1:300011/1‑149 (MQ=255)
                                              gCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCg                                                                                                       <  7:93009/149‑1 (MQ=255)
                                                   tCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAgg                                                                                                  >  6:182071/1‑149 (MQ=255)
                                                       gAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCg                                                                                               >  2:52564/1‑148 (MQ=255)
                                                          cGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAAc                                                                                           >  5:86820/1‑149 (MQ=255)
                                                              aGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGc                                                                                       <  1:252566/149‑1 (MQ=255)
                                                               gggCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGcc                                                                                      <  3:384058/149‑1 (MQ=255)
                                                               gggCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGc                                                                                       >  5:48931/1‑148 (MQ=255)
                                                                      gAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCa                                                                               >  6:215594/1‑149 (MQ=255)
                                                                       aaCGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCAc                                                                              <  4:136230/149‑1 (MQ=255)
                                                                        aCGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCAcc                                                                             >  4:23207/1‑149 (MQ=255)
                                                                            cTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGt                                                                         <  6:48931/149‑1 (MQ=255)
                                                                            cTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGt                                                                         >  3:128909/1‑149 (MQ=255)
                                                                                gCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAg                                                                     <  7:22944/149‑1 (MQ=255)
                                                                                 ccGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGa                                                                                              <  4:28669/123‑1 (MQ=255)
                                                                                 ccGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGa                                                                                              >  3:28669/1‑123 (MQ=255)
                                                                                  cGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGcc                                                                   <  8:211426/149‑1 (MQ=255)
                                                                                  cGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGcc                                                                   <  4:128909/149‑1 (MQ=255)
                                                                                      ttCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGAAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAg                                                               >  8:181395/1‑149 (MQ=255)
                                                                                        cAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGTGTCCAGCCACAgg                                                              <  6:86820/148‑1 (MQ=255)
                                                                                        cAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAgg                                                              <  2:94616/148‑1 (MQ=255)
                                                                                        cAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGgc                                                             >  8:249936/1‑149 (MQ=255)
                                                                                                       gTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCt                                              >  2:165502/1‑149 (MQ=255)
                                                                                                       gTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCt                                              >  7:201805/1‑149 (MQ=255)
                                                                                                            cGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCAGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTg                                         >  7:263004/1‑149 (MQ=255)
                                                                                                            cGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCAGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTg                                         >  7:268021/1‑149 (MQ=255)
                                                                                                                 gTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAg                                    >  8:300940/1‑149 (MQ=255)
                                                                                                                   gACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTgg                                            <  7:336521/139‑1 (MQ=255)
                                                                                                                   gACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTgg                                            >  8:336521/1‑139 (MQ=255)
                                                                                                                      cACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGAtct                               >  6:285953/1‑149 (MQ=255)
                                                                                                                       aCGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGAtctc                              >  6:118883/1‑149 (MQ=255)
                                                                                                                       aCGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGAtctc                              <  7:300940/149‑1 (MQ=255)
                                                                                                                           aTTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGATCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGcc                          >  1:111643/1‑149 (MQ=255)
                                                                                                                              cGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCg                       >  3:200190/1‑149 (MQ=255)
                                                                                                                              cGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCg                       >  7:4423/1‑149 (MQ=255)
                                                                                                                               gCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCgg                      <  3:23207/149‑1 (MQ=255)
                                                                                                                               gCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCg                       <  5:215594/148‑1 (MQ=255)
                                                                                                                               gCCTTTCACCTCGCCAGCGGCAGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCgg                      <  8:263004/149‑1 (MQ=255)
                                                                                                                               gCCTTTCACCTCGCCAGCGGCAGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCgg                      <  8:268021/149‑1 (MQ=255)
                                                                                                                                    tCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCGGCCATg                 >  8:44796/1‑149 (MQ=255)
                                                                                                                                      aCCTCGCCAGCGGCGGAAGGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCGGc                     >  7:222243/1‑143 (MQ=255)
                                                                                                                                      aCCTCGCCAGCGGCGGAAGGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCGGc                     <  8:222243/143‑1 (MQ=255)
                                                                                                                                      aCCTCGCCAGCGGCGGAAAGGAAGTTGAAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCGGCCATGc                <  7:181395/148‑1 (MQ=255)
                                                                                                                                           gCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCGGCCATGCCCGGc           <  5:323325/148‑1 (MQ=255)
                                                                                                                                             cAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCGGCCATGCCCGGCaga        <  5:182071/149‑1 (MQ=255)
                                                                                                                                               gcggcgGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCGGCCATGCCCGGCAGAGt      <  8:55207/149‑1 (MQ=255)
                                                                                                                                                cggcggAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCGGCCATGCCCGGCAGAGTg     <  6:255056/149‑1 (MQ=255)
                                                                                                                                                cggcggAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCGGCCATGCCCGGCAGAGTg     <  3:46185/149‑1 (MQ=255)
                                                                                                                                                  gcggAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCGGCCATGCCCGGCAGAGTGGc   <  1:165502/149‑1 (MQ=255)
                                                                                                                                                   cggAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCGGCCATGCCCGGCAGAGTGGcc  <  1:227268/149‑1 (MQ=255)
                                                                                                                                                   cggAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCGGCCATGCCCGGCAGAGTGGc   >  6:142432/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCATCTCGACCCGCGCCACATCGCCCAGCAGCACGCGGCTGTCACCGCTGGTCTTGAGCGGCAGGGCGGCGAACGCCTCGGCCGACTTCAGCTCGGTGCTGGCGTTGACGCTGGTGACCACGTATTCGCCTTTCACCTCGCCAGCGGCGGAAAGGAAGTTGTAGCGGCGTACGGCATTGGTCACGTCGGTAGCCGAAAGGCCGAAACCGGCCAGCTTCACCGGGTCCAGCCACAGGCGCATGGCGAACACCTGGTTGCCGAGGATCTCCGCCTCGGCCATGCCCGGCAGAGTGGCC  >  NC_002947/1047588‑1047883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑