Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,060,961 A→G 6.4% E56G (GAG→GGG)  PP_0918 → 3‑beta hydroxysteroid dehydrogenase/isomerase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,060,9610AG 6.4% 228.9 / 2.4 77E56G (GAG→GGG) PP_09183‑beta hydroxysteroid dehydrogenase/isomerase family protein
Reads supporting (aligned to +/- strand):  ref base A (33/39);  new base G (2/3);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGCGAGTGGCTTCATTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCTGCCTGAAAGAGCACG  >  NC_002947/1060813‑1061104
                                                                                                                                                    |                                                                                                                                               
ggCGCGAGTGGCTTCATTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGa                                                                                                                                                 <  1:267201/149‑1 (MQ=255)
  cgcgAGTGGCTTCATTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGc                                                                                                                                               <  3:397713/149‑1 (MQ=255)
         ggCTTCATTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCg                                                                                                                                        >  6:104089/1‑149 (MQ=255)
          gCTTCATTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGGCGCCGAGCTGGCCCgg                                                                                                                                       >  2:50921/1‑149 (MQ=255)
          gCTTCATTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCgg                                                                                                                                       >  7:102634/1‑149 (MQ=255)
          gCTTCATTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCgg                                                                                                                                       >  1:404320/1‑149 (MQ=255)
          gCTTCATTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCgg                                                                                                                                       <  1:88073/149‑1 (MQ=255)
           ctgcATTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGc                                                                                                                                      <  7:340135/146‑1 (MQ=255)
            ttCATTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCg                                                                                                                                     >  3:161605/1‑149 (MQ=255)
            ttCATTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCg                                                                                                                                     >  4:228292/1‑149 (MQ=255)
              cATTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGt                                                                                                                                    <  3:162367/148‑1 (MQ=255)
               aTTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGATCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGttt                                                                                                                                  >  7:262891/1‑149 (MQ=255)
               aTTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGttt                                                                                                                                  <  4:161605/149‑1 (MQ=255)
                ttGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGGGCCAGCTCGGCGtcac                                                                                                                                 <  5:362491/149‑4 (MQ=255)
                gtGGCGGGCGTTTTGCGCGTTTCGCCCGGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGGCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGGGCCAGCTCGGCGtcac                                                                                                                                 <  4:87224/148‑4 (MQ=255)
                  ggcgggcgTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTtgt                                                                                                                                <  1:316678/148‑1 (MQ=255)
                     gggcgTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGc                                                                                                                              >  3:58181/1‑147 (MQ=255)
                     gggcgTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCa                                                                                                                            <  5:28459/149‑1 (MQ=255)
                       gcgTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAgg                                                                                                                          <  5:53632/149‑1 (MQ=255)
                       gcgTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAgg                                                                                                                          <  3:228292/149‑1 (MQ=255)
                       gcgTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGGCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAgg                                                                                                                          <  2:227394/149‑1 (MQ=255)
                       gagTTTTGCGCGTTTCGCACTGGAGAAAGGCCTGGACGTGCGCGTCAGCGGACGCCGTGCCGAAGGGGTAGAGCATCTGGTCAATCGTGGCGCACAGTTCATAACGGGTGATCTGGGTGACGCAGATCTGGCCCGGCGTTTGTGCCAgt                                                                                                                          >  2:208327/3‑148 (MQ=255)
                          ttttGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGgtg                                                                                                                       >  5:20279/1‑149 (MQ=255)
                               cgcgTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGaa                                                                                                                  <  2:82660/149‑1 (MQ=255)
                               cgcgTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGa                                                                                                                   <  2:131119/148‑1 (MQ=255)
                                gcgTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAg                                                                                                                 >  4:273281/1‑149 (MQ=255)
                                     tCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCgt                                                                                                             <  6:111515/148‑1 (MQ=255)
                                     tCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCgt                                                                                                             <  6:209926/148‑1 (MQ=255)
                                       gCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCgtggt                                                                                                          <  6:174080/149‑1 (MQ=255)
                                       gCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCgtggt                                                                                                          <  3:273281/149‑1 (MQ=255)
                                       gCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCgtgg                                                                                                           <  6:289713/148‑1 (MQ=255)
                                       gCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCgtgg                                                                                                           <  5:104089/148‑1 (MQ=255)
                                          cTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCa                                                                                                       >  1:23727/1‑149 (MQ=255)
                                            ggAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCAtt                                                                                                     >  2:370560/1‑149 (MQ=255)
                                                      cTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGgcgc                                                                                           >  1:186111/1‑149 (MQ=255)
                                                         gACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGt                                                                                        <  1:370560/149‑1 (MQ=255)
                                                                  gTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTg                                                                               >  2:312023/1‑149 (MQ=255)
                                                                    cAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTggg                                                                             >  2:207118/1‑149 (MQ=255)
                                                                     aGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCGGTGGTGCATTGCGCTGGCGCGGTGGGTAACTggg                                                                             <  8:102634/148‑1 (MQ=255)
                                                                     aGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTggg                                                                             <  2:404320/148‑1 (MQ=255)
                                                                      gCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTggggg                                                                           <  8:175070/149‑1 (MQ=255)
                                                                          ccgccgTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGgcgc                                                                        >  5:286989/1‑148 (MQ=255)
                                                                             ccgTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTAt                                                                     >  2:96831/1‑148 (MQ=255)
                                                                                  ccGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGGGACGCCGAGCTTGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTTCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGAt                                                               <  5:288842/149‑1 (MQ=255)
                                                                                   cGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCGTTGCGGTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGAtg                                                              >  4:235523/1‑148 (MQ=255)
                                                                                        ggggCGAGCATCTGGTCAAGCGGGGCGCACAGTTCATCCCGGGTTATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTAc                                                         <  3:358774/149‑1 (MQ=255)
                                                                                                      gTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGTGTGACGCCGGGCTGGCACGGCGTTTGTGCCAGCGTGTCGAAGCCgt                                                                                                             >  7:41686/1‑83 (MQ=255)
                                                                                                      gTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCGAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACgtg                                             <  8:262891/147‑1 (MQ=255)
                                                                                                      gTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACgtggt                                           >  2:355458/1‑149 (MQ=255)
                                                                                                       tCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGGCGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACgtggtg                                          <  1:50921/149‑1 (MQ=255)
                                                                                                           gCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGa                                      <  4:58181/149‑1 (MQ=255)
                                                                                                                 cgcACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTGTACCAAGGCAACGTGGTGGTGACCgaga                                <  1:228958/149‑1 (MQ=255)
                                                                                                                 cgcACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCgaga                                <  1:355458/149‑1 (MQ=255)
                                                                                                                    acaGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGTCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTTGGGCATTGCGCTGGCGCGGTGGGTAACTGGGTGCGCTATCAGGATTTCTACAAAGGCAACGTGGTGGTGACCGAGAACg                             <  1:208327/149‑1 (MQ=255)
                                                                                                                       gTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTgg                          <  2:186111/149‑1 (MQ=255)
                                                                                                                       gTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTgg                          >  3:120771/1‑149 (MQ=255)
                                                                                                                           aTCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTgg                                                                              >  5:11991/1‑93 (MQ=255)
                                                                                                                           aTCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTgg                                                                              <  6:11991/93‑1 (MQ=255)
                                                                                                                              ccGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAgg                    <  6:286989/148‑1 (MQ=255)
                                                                                                                                gggTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCt                 >  6:222845/1‑149 (MQ=255)
                                                                                                                                    gATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAgggacgtgc             >  3:166081/1‑143 (MQ=255)
                                                                                                                                     aTCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCTGCCt             <  1:96831/148‑1 (MQ=255)
                                                                                                                                      tCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCTGCCTg            >  3:289539/1‑148 (MQ=255)
                                                                                                                                       cTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCTGCCTGa           >  6:3556/1‑148 (MQ=255)
                                                                                                                                       cTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCTGCCTGa           >  3:227395/1‑148 (MQ=255)
                                                                                                                                        tGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCTGCCTGa           >  1:178626/1‑147 (MQ=255)
                                                                                                                                         gggTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAAc                                                                                 <  1:197796/76‑1 (MQ=255)
                                                                                                                                         gggTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAAc                                                                                 >  2:197796/1‑76 (MQ=255)
                                                                                                                                         gggTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCTGCCTGa           >  8:382711/1‑146 (MQ=255)
                                                                                                                                          ggTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCTGCCTGAAaga       <  5:222845/149‑1 (MQ=255)
                                                                                                                                           gTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCTGCCTGAAagag      >  3:60493/1‑149 (MQ=255)
                                                                                                                                            tGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTTGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCTGCCTGAAAGAGc     <  6:20279/149‑1 (MQ=255)
                                                                                                                                            tGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCTGCCTGAAAGAGc     <  4:227395/149‑1 (MQ=255)
                                                                                                                                            tGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTAGTGACCGAGAACGTGGTCGAGGGCTGCCTGAAagag      >  7:73190/1‑148 (MQ=255)
                                                                                                                                              aCGCCGGGCCAGCTCGGCGTTTGTGCCAGGGTGTCGAAGCCGTTGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAg                                                     <  7:351879/99‑1 (MQ=255)
                                                                                                                                              aCGCCGGGCCAGCTCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAg                                                     >  8:351879/1‑99 (MQ=255)
                                                                                                                                              aCGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCTGCCTGAAAGAGCAc   <  1:207118/149‑1 (MQ=255)
                                                                                                                                                gCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCTGCCTGAAAGAGCACg  >  2:121878/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GGCGCGAGTGGCTTCATTGGCGGGCGTTTTGCGCGTTTCGCCCTGGAGCAAGGCCTGGACGTGCGCGTCAGCGGCCGCCGTGCCGAAGGGGTCGAGCATCTGGTCAAGCGTGGCGCACAGTTCATCCCGGGTGATCTGGGTGACGCCGAGCTGGCCCGGCGTTTGTGCCAGGGTGTCGAAGCCGTGGTGCATTGCGCTGGCGCGGTGGGTAACTGGGGGCGCTATCAGGATTTCTACCAAGGCAACGTGGTGGTGACCGAGAACGTGGTCGAGGGCTGCCTGAAAGAGCACG  >  NC_002947/1060813‑1061104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑