Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,115,626	A→T	6.6%	L736M (TTG→ATG) ‡	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,115,626	0	A	T	6.6%	224.7 / 4.0	75	L736M (TTG→ATG) ‡	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (34/36);  new base T (3/2);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

CTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAGCGGAACTGCTC  >  NC_002947/1115482‑1115770                                                                                                                                                 |                                                                                                                                                 cTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGt                                                                                                                                               <  1:400963/148‑1 (MQ=255)         gCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACTGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGt                                                                                                                                      >  4:315763/1‑149 (MQ=255)           cACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGAGTGc                                                                                                                                    <  7:126414/149‑1 (MQ=255)                gCCATGGCTGCAGCATGATGGTCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACgg                                                                                                                               <  4:76994/149‑1 (MQ=255)                gCCATGGCTGCAGCATGATGGTCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACg                                                                                                                                <  3:395306/148‑1 (MQ=255)                             caTGATGGTCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACggg                                                                                                                              >  3:16534/1‑137 (MQ=255)                             caTGATGGTCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACggg                                                                                                                              <  4:16534/137‑1 (MQ=255)                                gatgGTCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCgggg                                                                                                               >  1:26278/1‑149 (MQ=255)                                  tgGTCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGc                                                                                                              <  3:315763/148‑1 (MQ=255)                                     tCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGgcgcg                                                                                                                          <  5:5707/133‑1 (MQ=255)                                     tCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGgcgcg                                                                                                                          >  6:5707/1‑133 (MQ=255)                                     tCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGttt                                                                                                          >  2:234761/1‑149 (MQ=255)                                     tCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGttt                                                                                                          <  5:262798/149‑1 (MQ=255)                                     tCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCGGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGttt                                                                                                          <  1:229551/149‑1 (MQ=255)                                         gCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGt                                                                                                                                               >  3:259893/1‑108 (MQ=255)                                         gCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGt                                                                                                                                               <  4:259893/108‑1 (MQ=255)                                         gCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTc                                                                                                        <  2:26278/147‑1 (MQ=255)                                         gCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTc                                                                                                        <  8:228410/147‑1 (MQ=255)                                         gCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGt                                                                                                      >  6:65455/1‑149 (MQ=255)                                           cGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTcc                                                                                                    <  3:71434/149‑1 (MQ=255)                                           cGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTcc                                                                                                    <  2:338644/149‑1 (MQ=255)                                            gTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTcc                                                                                                    >  4:187153/1‑148 (MQ=255)                                                 tGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATCAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTGCCACAg                                                                                               >  1:406070/1‑148 (MQ=255)                                                    cGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTAcc                                                                                           >  3:358689/1‑149 (MQ=255)                                                     ggCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCGCTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCg                                                                                          >  6:408548/1‑149 (MQ=255)                                                     ggCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCg                                                                                          <  4:358689/149‑1 (MQ=255)                                                       ccAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGt                                                                                        <  4:45321/149‑1 (MQ=255)                                                          gcggcgCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTACCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTg                                                                                     <  1:298507/149‑1 (MQ=255)                                                            ggcgCGATGCGCTGCCAGATTTCTTCGGGGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGa                                                                                   <  3:170685/149‑1 (MQ=255)                                                             gcgcGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGa                                                                                   >  2:11568/1‑148 (MQ=255)                                                              cgcgATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTg                                                                                     <  8:253066/145‑1 (MQ=255)                                                              cgcgATGCGCTGCCAGATTTCTTCAGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTg                                                                                     >  7:253066/1‑145 (MQ=255)                                                                      gcTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTcc                                                                            >  8:221473/1‑146 (MQ=255)                                                                      gcTGCCAGATTTCTTCGGGGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCGCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTcc                                                                            <  7:221473/146‑1 (MQ=255)                                                                      gatGCCAGATTTCTTCTGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAgg                                                                         <  1:370266/147‑1 (MQ=255)                                                                            aGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCggg                                                                                                                                       >  6:263636/1‑81 (MQ=255)                                                                            aGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCggg                                                                                                                                       <  5:263636/81‑1 (MQ=255)                                                                                   ttcGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAg                                                             >  3:224448/1‑148 (MQ=255)                                                                                   ttcGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAg                                                             >  1:4714/1‑148 (MQ=255)                                                                                     cGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTAc                                                          >  7:268071/1‑149 (MQ=255)                                                                                          atgaACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGt                                                      <  5:65455/148‑1 (MQ=255)                                                                                             aaCGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGa                                                                                   >  1:268447/1‑116 (MQ=255)                                                                                             aaCGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGa                                                                                   <  2:268447/116‑1 (MQ=255)                                                                                             aaCGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTcc                                                   <  4:224448/148‑1 (MQ=255)                                                                                                ggCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGAt                                               <  7:38192/149‑1 (MQ=255)                                                                                                     gAACGGATGCGCAAGCCGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTGTGGACAGCTCCAGGTACCAGTCGCAGTACTTGTTCCCGATAAACt                                          >  8:58961/1‑149 (MQ=255)                                                                                                     gAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCa                                                              <  4:334815/129‑1 (MQ=255)                                                                                                     gAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCa                                                              >  3:334815/1‑129 (MQ=255)                                                                                                           aTGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTa                                      >  5:89446/1‑147 (MQ=255)                                                                                                               gcAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTg                                                        <  4:209168/125‑1 (MQ=255)                                                                                                               gcAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTg                                                        >  3:209168/1‑125 (MQ=255)                                                                                                                  aGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCctg                             <  8:268071/149‑1 (MQ=255)                                                                                                                      gCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCctggctg                         >  2:378576/1‑149 (MQ=255)                                                                                                                            ccACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAgg                   >  4:124986/1‑149 (MQ=255)                                                                                                                                  ccAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAg             >  7:279328/1‑149 (MQ=255)                                                                                                                                   cacccgccgcACCTTGGTGC‑GCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAGCg           >  7:187086/8‑149 (MQ=255)                                                                                                                                   cAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTgg                                                       >  8:236237/1‑106 (MQ=255)                                                                                                                                   cAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTgg                                                       <  7:236237/106‑1 (MQ=255)                                                                                                                                   cAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACa                                    >  1:102190/1‑125 (MQ=255)                                                                                                                                   cAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACa                                    <  2:102190/125‑1 (MQ=255)                                                                                                                                   cAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAGc            <  3:325409/149‑1 (MQ=255)                                                                                                                                    acccgccgcACCTTGGTGC‑GCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAGCgg          <  8:187086/143‑1 (MQ=255)                                                                                                                                    aGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAGCg           >  2:312395/1‑149 (MQ=255)                                                                                                                                    aGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAGCg           >  8:151159/1‑149 (MQ=255)                                                                                                                                      cACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAGcctt         <  1:234761/149‑4 (MQ=255)                                                                                                                                      cACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGACCAGGTCAAAGCgg          >  3:199630/1‑148 (MQ=255)                                                                                                                                       cgccgcACCTTGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAGCGGaa        >  8:339650/4‑149 (MQ=255)                                                                                                                                       cgccgcACCTTGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAGCGGaa        >  4:199130/4‑149 (MQ=255)                                                                                                                                         gcgcACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAGCGGAACt      >  5:232135/1‑149 (MQ=255)                                                                                                                                         gcgcACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAGCGGAACt      >  8:18094/1‑149 (MQ=255)                                                                                                                                           gcACCTTGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAGCGGAACTGc    <  3:199130/149‑1 (MQ=255)                                                                                                                                             aCCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAGCGGAACTGCTc  <  8:279328/149‑1 (MQ=255)                                                                                                                                             aCCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGACCAGGTCAAAGCGGAACTGCTc  <  4:199630/149‑1 (MQ=255)                                                                                                                                               cAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCaaa              <  2:344288/135‑1 (MQ=255)                                                                                                                                               cAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCaaa              >  1:344288/1‑135 (MQ=255)                                                                                                                                                 |                                                                                                                                                 CTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGATGCCGGCCAGCGGCGCGATGCGCTGCCAGATTTCTTCGGTGATGAACGGCATGAACGGATGCGCAAGACGCAGTGCCACTTCCAGCACGCGCACCAAGGTGCGGCGGGTGCCACGGGCGCGCTCTACCGGGGCGTTTTCGTCCCACAGTACCGGTTTGGACAGCTCCAGGTACCAGTCGCAGTACTGGTTCCAGATAAACTCGTACAGCGCCTGGCTGGCCAGGTCAAAGCGGAACTGCTC  >  NC_002947/1115482‑1115770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑