Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,145,433 C→T 12.5% intergenic (‑100/+41) hemO ← / ← PP_1006 heme oxygenase/heme receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,145,4330CT12.5% 122.0 / 7.6 48intergenic (‑100/+41)hemO/PP_1006heme oxygenase/heme receptor
Reads supporting (aligned to +/- strand):  ref base C (21/21);  new base T (4/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

GTGATCTGGTTCAGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGC  >  NC_002947/1145287‑1145577
                                                                                                                                                    |                                                                                                                                                 
gTGATATGGTTCAGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTcgc                                                                                                                                                 >  3:45070/1‑149 (MQ=255)
    tCTGGTTCAGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTACGTCTGTACCGGCCTCTTTGCgg                                                                                                                                               >  2:270632/1‑149 (MQ=255)
     cTGGTTCAGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGc                                                                                                                                             <  8:14732/148‑1 (MQ=255)
           cAGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGccc                                                                                                                                       <  6:66074/148‑1 (MQ=255)
                   gggAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTACGTCTGTACCGGCCTCTTTGCGGGCACGCCCGCGAaga                                                                                                                                <  1:270632/149‑1 (MQ=37)
                    ggAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTTGCGGGCACGCCCGCGAagag                                                                                                                               <  3:93445/147‑1 (MQ=255)
                    ggAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGc                                                                                                                             <  2:119157/149‑1 (MQ=255)
                        cgcAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGTAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGt                                                                                                                         <  8:176606/149‑1 (MQ=255)
                                tGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACg                                                                                                                  >  2:66082/1‑148 (MQ=255)
                                  ggCGGTCGGTCATGGGGTAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTaa                                                                                                               <  8:93257/149‑1 (MQ=255)
                                    cggtcggtCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACg                                                                                                             >  6:166388/1‑149 (MQ=255)
                                     ggtcggtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGc                                                                                                            <  8:369552/149‑1 (MQ=255)
                                         ggtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCt                                                                                                          >  5:362608/1‑147 (MQ=255)
                                          gtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAg                                                                                                       <  5:66115/149‑1 (MQ=255)
                                               gggggAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATc                                                                                                  >  7:194038/1‑149 (MQ=255)
                                               gggggAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATc                                                                                                  >  3:352537/1‑149 (MQ=255)
                                                     gTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACATCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCGGCAAAGAGGCCGGTACAGACGTAACGCCTCa                                                                                                        <  4:191249/137‑1 (MQ=255)
                                                     gTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCa                                                                                                        >  3:191249/1‑137 (MQ=255)
                                                      tCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAAc                                                                                            <  8:194038/148‑1 (MQ=255)
                                                        cTTGGAAAGAGGGCGTCTAGATACAATACGAAACAGCGGGAGCGAGACAGTAAAAAAATCCGGTATTTTCGTT‑‑GCCAGTACCGGCCTCTTCGAGGGCGTGAACGCAAAGAGGACGGTACAGACGTAACGCCTCAGATATCCCAAAccc                                                                                          >  5:292518/1‑147 (MQ=255)
                                                                  gggCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAg                                                                                                       >  6:368828/1‑125 (MQ=255)
                                                                  gggCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAg                                                                                                       <  5:368828/125‑1 (MQ=255)
                                                                      gTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATAc                                                                                 <  6:96902/143‑1 (MQ=255)
                                                                      gTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATAc                                                                                 >  5:96902/1‑143 (MQ=255)
                                                                      gTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGt                                                                          >  6:33004/1‑149 (MQ=255)
                                                                              aCAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGcccc                                                                  >  5:293680/1‑149 (MQ=255)
                                                                                aaGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCgg                                                                >  7:298028/1‑149 (MQ=255)
                                                                                    cGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATg                                                            <  6:293680/149‑1 (MQ=255)
                                                                                       aaCAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGt                                                          >  3:291022/1‑148 (MQ=255)
                                                                                                aGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCg                                                <  4:352537/149‑1 (MQ=255)
                                                                                                  cGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGAt                                              <  2:216026/149‑1 (MQ=255)
                                                                                                    agaCAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGt                                            <  4:45070/149‑1 (MQ=255)
                                                                                                       cAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGCAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtg                                          <  1:66082/149‑1 (MQ=255)
                                                                                                       cAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGAt                                              >  3:133200/1‑144 (MQ=255)
                                                                                                       cAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGAt                                              <  4:133200/144‑1 (MQ=255)
                                                                                                        aGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggc                                        >  5:96435/1‑149 (MQ=255)
                                                                                                        aGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggc                                        <  5:33004/149‑1 (MQ=255)
                                                                                                                  gCCTGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCTGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCTGTCGATGTTGGCTGGCTGGGTc                              <  6:292518/149‑1 (MQ=255)
                                                                                                                    cGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTca                             >  2:11321/1‑148 (MQ=255)
                                                                                                                    cGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTca                             >  1:70588/1‑148 (MQ=255)
                                                                                                                      gTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAg                          >  5:88704/1‑149 (MQ=255)
                                                                                                                       tATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAgc                         <  5:166388/149‑1 (MQ=255)
                                                                                                                         tttGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAgccg                       >  1:336849/1‑149 (MQ=255)
                                                                                                                           tGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAgccgcc                     >  6:328374/1‑149 (MQ=255)
                                                                                                                                      aGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTgg            <  2:70588/149‑1 (MQ=255)
                                                                                                                                               ctctTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAg   >  2:47561/1‑149 (MQ=255)
                                                                                                                                               ctctTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAg   >  7:225665/1‑149 (MQ=255)
                                                                                                                                                tctTTGCGGGCACGCCCGCGAAGAGGCCGGTACTGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGc  >  2:294938/1‑149 (MQ=39)
                                                                                                                                                tctTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGc  >  3:136796/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GTGATCTGGTTCAGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGC  >  NC_002947/1145287‑1145577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑