Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,145,441 T→C 10.6% intergenic (‑108/+33) hemO ← / ← PP_1006 heme oxygenase/heme receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,145,4410TC10.6% 120.7 / 5.8 47intergenic (‑108/+33)hemO/PP_1006heme oxygenase/heme receptor
Reads supporting (aligned to +/- strand):  ref base T (21/21);  new base C (3/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01

CAGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGA  >  NC_002947/1145298‑1145587
                                                                                                                                                 |                                                                                                                                                   
cAGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGccc                                                                                                                                                 <  6:66074/148‑1 (MQ=255)
        gggAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTACGTCTGTACCGGCCTCTTTGCGGGCACGCCCGCGAaga                                                                                                                                          <  1:270632/149‑1 (MQ=37)
         ggAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTTGCGGGCACGCCCGCGAagag                                                                                                                                         <  3:93445/147‑1 (MQ=255)
         ggAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGc                                                                                                                                       <  2:119157/149‑1 (MQ=255)
             cgcAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGTAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGt                                                                                                                                   <  8:176606/149‑1 (MQ=255)
                     tGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACg                                                                                                                            >  2:66082/1‑148 (MQ=255)
                       ggCGGTCGGTCATGGGGTAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTaa                                                                                                                         <  8:93257/149‑1 (MQ=255)
                         cggtcggtCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACg                                                                                                                       >  6:166388/1‑149 (MQ=255)
                          ggtcggtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGc                                                                                                                      <  8:369552/149‑1 (MQ=255)
                              ggtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCt                                                                                                                    >  5:362608/1‑147 (MQ=255)
                               gtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAg                                                                                                                 <  5:66115/149‑1 (MQ=255)
                                    gggggAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATc                                                                                                            >  7:194038/1‑149 (MQ=255)
                                    gggggAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATc                                                                                                            >  3:352537/1‑149 (MQ=255)
                                          gTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACATCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCGGCAAAGAGGCCGGTACAGACGTAACGCCTCa                                                                                                                  <  4:191249/137‑1 (MQ=255)
                                          gTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCa                                                                                                                  >  3:191249/1‑137 (MQ=255)
                                           tCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAAc                                                                                                      <  8:194038/148‑1 (MQ=255)
                                             cTTGGAAAGAGGGCGTCTAGATACAATACGAAACAGCGGGAGCGAGACAGTAAAAAAATCCGGTATTTTCGTT‑‑GCCAGTACCGGCCTCTTCGAGGGCGTGAACGCAAAGAGGACGGTACAGACGTAACGCCTCAGATATCCCAAAccc                                                                                                    >  5:292518/1‑147 (MQ=255)
                                                       gggCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAg                                                                                                                 >  6:368828/1‑125 (MQ=255)
                                                       gggCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAg                                                                                                                 <  5:368828/125‑1 (MQ=255)
                                                           gTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATAc                                                                                           >  5:96902/1‑143 (MQ=255)
                                                           gTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATAc                                                                                           <  6:96902/143‑1 (MQ=255)
                                                           gTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGt                                                                                    >  6:33004/1‑149 (MQ=255)
                                                                   aCAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGcccc                                                                            >  5:293680/1‑149 (MQ=255)
                                                                     aaGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCgg                                                                          >  7:298028/1‑149 (MQ=255)
                                                                         cGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATg                                                                      <  6:293680/149‑1 (MQ=255)
                                                                            aaCAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGt                                                                    >  3:291022/1‑148 (MQ=255)
                                                                                     aGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCg                                                          <  4:352537/149‑1 (MQ=255)
                                                                                       cGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGAt                                                        <  2:216026/149‑1 (MQ=255)
                                                                                         agaCAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGt                                                      <  4:45070/149‑1 (MQ=255)
                                                                                            cAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGCAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtg                                                    <  1:66082/149‑1 (MQ=255)
                                                                                            cAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGAt                                                        >  3:133200/1‑144 (MQ=255)
                                                                                            cAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGAt                                                        <  4:133200/144‑1 (MQ=255)
                                                                                             aGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggc                                                  <  5:33004/149‑1 (MQ=255)
                                                                                             aGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggc                                                  >  5:96435/1‑149 (MQ=255)
                                                                                                       gCCTGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCTGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCTGTCGATGTTGGCTGGCTGGGTc                                        <  6:292518/149‑1 (MQ=255)
                                                                                                         cGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTca                                       >  1:70588/1‑148 (MQ=255)
                                                                                                         cGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTca                                       >  2:11321/1‑148 (MQ=255)
                                                                                                           gTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAg                                    >  5:88704/1‑149 (MQ=255)
                                                                                                            tATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAgc                                   <  5:166388/149‑1 (MQ=255)
                                                                                                              tttGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAgccg                                 >  1:336849/1‑149 (MQ=255)
                                                                                                                tGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAgccgcc                               >  6:328374/1‑149 (MQ=255)
                                                                                                                           aGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTgg                      <  2:70588/149‑1 (MQ=255)
                                                                                                                                    ctctTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAg             >  2:47561/1‑149 (MQ=255)
                                                                                                                                    ctctTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAg             >  7:225665/1‑149 (MQ=255)
                                                                                                                                     tctTTGCGGGCACGCCCGCGAAGAGGCCGGTACTGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGc            >  2:294938/1‑149 (MQ=39)
                                                                                                                                     tctTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGc            >  3:136796/1‑149 (MQ=255)
                                                                                                                                               cGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCCCCCTGGCCGTCGTAGCTGCGCACCGa  <  1:11321/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CAGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGA  >  NC_002947/1145298‑1145587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑