Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,145,448 A→G 10.0% intergenic (‑115/+26) hemO ← / ← PP_1006 heme oxygenase/heme receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,145,4480AG10.0% 129.4 / 5.9 50intergenic (‑115/+26)hemO/PP_1006heme oxygenase/heme receptor
Reads supporting (aligned to +/- strand):  ref base A (23/22);  new base G (3/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

GGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATCCCACT  >  NC_002947/1145306‑1145595
                                                                                                                                                |                                                                                                                                                    
gggAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTACGTCTGTACCGGCCTCTTTGCGGGCACGCCCGCGAaga                                                                                                                                                  <  1:270632/149‑1 (MQ=37)
 ggAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTTGCGGGCACGCCCGCGAagag                                                                                                                                                 <  3:93445/147‑1 (MQ=255)
 ggAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGc                                                                                                                                               <  2:119157/149‑1 (MQ=255)
     cgcAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGTAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGt                                                                                                                                           <  8:176606/149‑1 (MQ=255)
             tGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACg                                                                                                                                    >  2:66082/1‑148 (MQ=255)
               ggCGGTCGGTCATGGGGTAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTaa                                                                                                                                 <  8:93257/149‑1 (MQ=255)
                 cggtcggtCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACg                                                                                                                               >  6:166388/1‑149 (MQ=255)
                  ggtcggtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGc                                                                                                                              <  8:369552/149‑1 (MQ=255)
                      ggtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCt                                                                                                                            >  5:362608/1‑147 (MQ=255)
                       gtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAg                                                                                                                         <  5:66115/149‑1 (MQ=255)
                            gggggAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATc                                                                                                                    >  7:194038/1‑149 (MQ=255)
                            gggggAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATc                                                                                                                    >  3:352537/1‑149 (MQ=255)
                                  gTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACATCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCGGCAAAGAGGCCGGTACAGACGTAACGCCTCa                                                                                                                          <  4:191249/137‑1 (MQ=255)
                                  gTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCa                                                                                                                          >  3:191249/1‑137 (MQ=255)
                                   tCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAAc                                                                                                              <  8:194038/148‑1 (MQ=255)
                                     cTTGGAAAGAGGGCGTCTAGATACAATACGAAACAGCGGGAGCGAGACAGTAAAAAAATCCGGTATTTTCGTT‑‑GCCAGTACCGGCCTCTTCGAGGGCGTGAACGCAAAGAGGACGGTACAGACGTAACGCCTCAGATATCCCAAAccc                                                                                                            >  5:292518/1‑147 (MQ=255)
                                               gggCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAg                                                                                                                         >  6:368828/1‑125 (MQ=255)
                                               gggCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAg                                                                                                                         <  5:368828/125‑1 (MQ=255)
                                                   gTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATAc                                                                                                   >  5:96902/1‑143 (MQ=255)
                                                   gTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATAc                                                                                                   <  6:96902/143‑1 (MQ=255)
                                                   gTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGt                                                                                            >  6:33004/1‑149 (MQ=255)
                                                           aCAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGcccc                                                                                    >  5:293680/1‑149 (MQ=255)
                                                             aaGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCgg                                                                                  >  7:298028/1‑149 (MQ=255)
                                                                 cGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATg                                                                              <  6:293680/149‑1 (MQ=255)
                                                                    aaCAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGt                                                                            >  3:291022/1‑148 (MQ=255)
                                                                             aGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCg                                                                  <  4:352537/149‑1 (MQ=255)
                                                                               cGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGAt                                                                <  2:216026/149‑1 (MQ=255)
                                                                                 agaCAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGt                                                              <  4:45070/149‑1 (MQ=255)
                                                                                    cAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGCAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtg                                                            <  1:66082/149‑1 (MQ=255)
                                                                                    cAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGAt                                                                <  4:133200/144‑1 (MQ=255)
                                                                                    cAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGAt                                                                >  3:133200/1‑144 (MQ=255)
                                                                                     aGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggc                                                          <  5:33004/149‑1 (MQ=255)
                                                                                     aGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggc                                                          >  5:96435/1‑149 (MQ=255)
                                                                                               gCCTGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCTGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCTGTCGATGTTGGCTGGCTGGGTc                                                <  6:292518/149‑1 (MQ=255)
                                                                                                 cGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTca                                               >  2:11321/1‑148 (MQ=255)
                                                                                                 cGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTca                                               >  1:70588/1‑148 (MQ=255)
                                                                                                   gTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAg                                            >  5:88704/1‑149 (MQ=255)
                                                                                                    tATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAgc                                           <  5:166388/149‑1 (MQ=255)
                                                                                                      tttGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAgccg                                         >  1:336849/1‑149 (MQ=255)
                                                                                                        tGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAgccgcc                                       >  6:328374/1‑149 (MQ=255)
                                                                                                                   aGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTgg                              <  2:70588/149‑1 (MQ=255)
                                                                                                                            ctctTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAg                     >  2:47561/1‑149 (MQ=255)
                                                                                                                            ctctTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAg                     >  7:225665/1‑149 (MQ=255)
                                                                                                                             tctTTGCGGGCACGCCCGCGAAGAGGCCGGTACTGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGc                    >  2:294938/1‑149 (MQ=39)
                                                                                                                             tctTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGc                    >  3:136796/1‑149 (MQ=255)
                                                                                                                                       cGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCCCCCTGGCCGTCGTAGCTGCGCACCGa          <  1:11321/149‑1 (MQ=255)
                                                                                                                                            ccGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggct                                                         <  3:178683/97‑1 (MQ=255)
                                                                                                                                            ccGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggct                                                         >  4:178683/1‑97 (MQ=255)
                                                                                                                                              gCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATCCCAc   >  2:363808/1‑149 (MQ=255)
                                                                                                                                               cAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATCCCACt  <  6:190168/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTT‑‑GCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCG‑AAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATCCCACT  >  NC_002947/1145306‑1145595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑