| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,184,585 | G→C | 40.5% | intergenic (‑201/‑211) | mltF ← / → purL | membrane‑bound lytic murein transglycosylase F/phosphoribosylformylglycinamidine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,184,585 | 0 | G | C | 40.5% | 29.8 / 46.5 | 42 | intergenic (‑201/‑211) | mltF/purL | membrane‑bound lytic murein transglycosylase F/phosphoribosylformylglycinamidine synthase |
| Reads supporting (aligned to +/- strand): ref base G (18/7); new base C (9/8); total (27/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
GTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCG > NC_002947/1184440‑1184729 | gTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCcgcgcg < 6:105578/148‑1 (MQ=255) tgTAGCGGGTTGGGTGGTTGGGGGAGGGGTGATTGCATCAGGGCTGCGAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCccgcgcg < 8:353477/147‑1 (MQ=37) gtcgCGGGTTGTGTGGTGGGGGGAGGGGTGATTGCATCAGGGCTGCCAGGTGGGCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCgcgggc < 4:73028/143‑1 (MQ=255) gCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGg‑cgc < 1:312904/148‑1 (MQ=255) gggTTGGGTGGGTGGGGGAGGGGGGATGGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGG‑CGCTCg < 3:318174/149‑1 (MQ=37) gggAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGACATATAGCGCCGCGCGGGCGG‑CGCTCg > 3:397725/1‑134 (MQ=255) ggggggATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGG‑CGCt > 8:8426/1‑128 (MQ=255) ggggggATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGG‑CGCt < 7:8426/128‑1 (MQ=255) ggggACTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCTGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCCGCTCGATCTCATAGGCGCTGAAtat < 1:5799/149‑3 (MQ=21) gggATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGggcgg‑c > 6:37669/1‑122 (MQ=255) gggATTGCATCAGGGCGGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGggcgg‑c < 5:37669/122‑1 (MQ=255) gTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGG‑CGCTCGAt < 7:47625/107‑1 (MQ=255) gTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGG‑CGCTCGAt > 8:47625/1‑107 (MQ=255) cTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAg > 2:131993/1‑106 (MQ=255) cTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAg < 1:131993/106‑1 (MQ=255) cgccgcCAGGTGTCCTCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCTCTCGATCTCGTCTCAAGCCGGACACCTCGGAAAACTGccc > 6:115187/1‑149 (MQ=255) cgccgcCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGAcc > 6:120943/1‑149 (MQ=255) gtgtCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTcc > 1:24815/1‑149 (MQ=255) ccTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTccg < 2:24815/149‑1 (MQ=255) cAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAgg > 2:256655/1‑149 (MQ=255) aGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCg > 2:289824/1‑123 (MQ=255) aGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGCCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCg < 1:289824/123‑1 (MQ=255) cgcCTGTGAGATCGAGCGCCGCCCGCGCGG‑CGCTCGATCTCATAGGCGCTGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGt > 6:374454/1‑149 (MQ=25) ccTATGAGATCGAGCGCCGTGCGGGCGG‑CGCTCGGTCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTgg > 1:55964/1‑149 (MQ=255) cTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGc > 4:133182/1‑149 (MQ=255) tATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTAGATCTCGACTCAACCCGGACACCTCAGAAAACTGACGCCGACGTCCCGATCCGCCGCCCGGCAGGCGTGGcg > 4:291788/1‑148 (MQ=38) aTGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCt > 6:168783/1‑148 (MQ=255) tGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGt > 4:148174/1‑119 (MQ=255) tGAGATCGAGCGCCGCCCGCGCGG‑CGCTCGATCTCATAGGCGCTGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCtt < 5:374454/148‑1 (MQ=255) agaTCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTa > 6:93084/1‑147 (MQ=255) gaTCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGAcc > 6:79020/1‑109 (MQ=255) gaTCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGAcc < 5:79020/109‑1 (MQ=255) gaTCGAGCGCCGCCCGCGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGc > 7:16858/1‑143 (MQ=255) gaTCGAGCGCCGCCCGCGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGc < 8:16858/143‑1 (MQ=255) aTCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTagag > 1:60432/1‑148 (MQ=255) tCGAGCGCCGCCCGCGCGG‑CGCTCGATCTCATAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTg < 7:60474/139‑1 (MQ=255) tCGAGCGCCGCCCGCGCGG‑CGCTCGATCTCATAGACGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTg > 8:60474/1‑139 (MQ=255) cGAGCGCCGCCCGCGCGG‑CGCTCGATCTCATAGGCGCTGAATATCTTAAGGCGGACACCTGGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTgg < 3:339863/140‑1 (MQ=17) cGAGCGCCGCCCGCGCGG‑CGCTCGATCTCATAGGCGCTGAATATCTTAAGGCGGACACCTGGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTgg > 4:339863/1‑140 (MQ=17) gAGCGCCGCCCGCGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAgg > 2:103632/1‑149 (MQ=255) aGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGt > 7:86730/1‑149 (MQ=255) aGCGCCGCCCGCGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGCCACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGt > 6:59460/1‑149 (MQ=255) gcgcCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTgg < 7:13138/136‑1 (MQ=255) gcgcCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTgg > 8:13138/1‑136 (MQ=255) gcgcCGCCCGCGCGT‑CGCTCGATCTCACAGTCGCAGAATGAGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGCCACCGCTGAACACAGACCCCGAAGACCGGTTCCGCCGCCCGGCAGGCGTTGCTGAGAGAAGGTg > 8:332121/1‑149 (MQ=255) cgcccgCGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCg > 4:147364/1‑148 (MQ=255) | GTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCG > NC_002947/1184440‑1184729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |