Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,184,612	A→T	11.1%	intergenic (‑228/‑184)	mltF ← / → purL	membrane‑bound lytic murein transglycosylase F/phosphoribosylformylglycinamidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,184,612	0	A	T	11.1%	115.1 / 2.6	43	intergenic (‑228/‑184)	mltF/purL	membrane‑bound lytic murein transglycosylase F/phosphoribosylformylglycinamidine synthase
Reads supporting (aligned to +/- strand):  ref base A (25/13);  new base T (2/3);  total (27/16)
Fisher's exact test for biased strand distribution p-value = 3.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGC  >  NC_002947/1184471‑1184760                                                                                                                                               |                                                                                                                                                      ggggACTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCTGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCCGCTCGATCTCATAGGCGCTGAAtat                                                                                                                                                 <  1:5799/149‑3 (MQ=21)                                                              cgccgcCAGGTGTCCTCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCTCTCGATCTCGTCTCAAGCCGGACACCTCGGAAAACTGccc                                                                                  >  6:115187/1‑149 (MQ=255)                                                              cgccgcCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGAcc                                                                                  >  6:120943/1‑149 (MQ=255)                                                                       gtgtCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTcc                                                                         >  1:24815/1‑149 (MQ=255)                                                                              ccTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTccg                                                                  <  2:24815/149‑1 (MQ=255)                                                                                          cAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAgg                                                      >  2:256655/1‑149 (MQ=255)                                                                                           aGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCg                                                                               >  2:289824/1‑123 (MQ=255)                                                                                           aGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGCCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCg                                                                               <  1:289824/123‑1 (MQ=255)                                                                                             cgcCTGTGAGATCGAGCGCCGCCCGCGCGG‑CGCTCGATCTCATAGGCGCTGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGt                                                   >  6:374454/1‑149 (MQ=25)                                                                                               ccTATGAGATCGAGCGCCGTGCGGGCGG‑CGCTCGGTCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTgg                                                 >  1:55964/1‑149 (MQ=255)                                                                                                cTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGc                                                >  4:133182/1‑149 (MQ=255)                                                                                                 tATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTAGATCTCGACTCAACCCGGACACCTCAGAAAACTGACGCCGACGTCCCGATCCGCCGCCCGGCAGGCGTGGcg                                               >  4:291788/1‑148 (MQ=38)                                                                                                  aTGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCt                                               >  6:168783/1‑148 (MQ=255)                                                                                                   tGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGt                                                                           >  4:148174/1‑119 (MQ=255)                                                                                                   tGAGATCGAGCGCCGCCCGCGCGG‑CGCTCGATCTCATAGGCGCTGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCtt                                              <  5:374454/148‑1 (MQ=255)                                                                                                     agaTCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTa                                             >  6:93084/1‑147 (MQ=255)                                                                                                      gaTCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGAcc                                                                                  >  6:79020/1‑109 (MQ=255)                                                                                                      gaTCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGAcc                                                                                  <  5:79020/109‑1 (MQ=255)                                                                                                      gaTCGAGCGCCGCCCGCGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGc                                                >  7:16858/1‑143 (MQ=255)                                                                                                      gaTCGAGCGCCGCCCGCGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGc                                                <  8:16858/143‑1 (MQ=255)                                                                                                       aTCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTagag                                          >  1:60432/1‑148 (MQ=255)                                                                                                        tCGAGCGCCGCCCGCGCGG‑CGCTCGATCTCATAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTg                                                  <  7:60474/139‑1 (MQ=255)                                                                                                        tCGAGCGCCGCCCGCGCGG‑CGCTCGATCTCATAGACGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTg                                                  >  8:60474/1‑139 (MQ=255)                                                                                                         cGAGCGCCGCCCGCGCGG‑CGCTCGATCTCATAGGCGCTGAATATCTTAAGGCGGACACCTGGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTgg                                                 <  3:339863/140‑1 (MQ=17)                                                                                                         cGAGCGCCGCCCGCGCGG‑CGCTCGATCTCATAGGCGCTGAATATCTTAAGGCGGACACCTGGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTgg                                                 >  4:339863/1‑140 (MQ=17)                                                                                                          gAGCGCCGCCCGCGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAgg                                      >  2:103632/1‑149 (MQ=255)                                                                                                           aGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGt                                     >  7:86730/1‑149 (MQ=255)                                                                                                           aGCGCCGCCCGCGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGCCACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGt                                     >  6:59460/1‑149 (MQ=255)                                                                                                            gcgcCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTgg                                                 >  8:13138/1‑136 (MQ=255)                                                                                                            gcgcCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTgg                                                 <  7:13138/136‑1 (MQ=255)                                                                                                            gcgcCGCCCGCGCGT‑CGCTCGATCTCACAGTCGCAGAATGAGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGCCACCGCTGAACACAGACCCCGAAGACCGGTTCCGCCGCCCGGCAGGCGTTGCTGAGAGAAGGTg                                    >  8:332121/1‑149 (MQ=255)                                                                                                                cgcccgCGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCg                                 >  4:147364/1‑148 (MQ=255)                                                                                                                         gg‑cgCTCGATCTCACTGGCGCAGACTGTGTCGAGCCGGAGACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCTGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCg                       <  7:332121/149‑1 (MQ=255)                                                                                                                               tCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGc                                   <  7:77542/132‑1 (MQ=255)                                                                                                                               tCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGc                                   >  8:77542/1‑132 (MQ=255)                                                                                                                                    ctcACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAg                                       >  4:173823/1‑123 (MQ=255)                                                                                                                                    ctcACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAg                                       <  3:173823/123‑1 (MQ=255)                                                                                                                                       acaGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAg                                       >  2:91975/1‑120 (MQ=255)                                                                                                                                       acaGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAg                                       <  1:91975/120‑1 (MQ=255)                                                                                                                                       acaGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTa             <  5:168783/146‑1 (MQ=255)                                                                                                                                       acaGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGaa          >  2:154347/1‑149 (MQ=255)                                                                                                                                       acaGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGa           <  1:256655/148‑1 (MQ=255)                                                                                                                                           gcgcAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCa      <  3:133182/149‑1 (MQ=255)                                                                                                                                               aGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGc  <  5:93084/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                      GGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGG‑CGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGC  >  NC_002947/1184471‑1184760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑