Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,201,597	A→T	6.0%	P135P (CCA→CCT)  M1M (ATG→TTG) †	xcpV → xcpW →	type II secretion pathway protein XcpV type II secretion pathway protein XcpW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,201,597	0	A	T	6.0%	213.9 / 2.9	72	P135P (CCA→CCT)  M1M (ATG→TTG) †	xcpV xcpW	type II secretion pathway protein XcpV type II secretion pathway protein XcpW
Reads supporting (aligned to +/- strand):  ref base A (27/39);  new base T (2/2);  total (29/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01

AGCTCGGCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTGCAGGACACCACTCGGCAAATCTCGG  >  NC_002947/1201449‑1201744                                                                                                                                                     |                                                                                                                                                    aGCTCGGCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCa                                                                                                                                                     <  2:216954/149‑1 (MQ=255)  gCTCGGCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCAt                                                                                                                                                    <  7:114178/149‑1 (MQ=255)      ggCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGa                                                                                                                                                  <  6:81213/147‑1 (MQ=255)        ccAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAaca                                                                                                                                              >  2:244085/1‑149 (MQ=255)          aaGGTGGCCAGCGGTGTCACTGGCAACTGCACCTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCATGGGCGCTCCAGAAacacg                                                                                                                                            <  8:313048/149‑2 (MQ=255)           aGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCATGGGCGCTCCAGAAacacgg                                                                                                                                           <  3:292339/149‑3 (MQ=255)            ggTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGc                                                                                                                                          <  8:314691/149‑1 (MQ=255)                gCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCaaa                                                                                                                                      <  5:139290/149‑1 (MQ=255)                  cAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAAcc                                                                                                                                    >  5:30478/1‑149 (MQ=255)                  cAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAAcc                                                                                                                                    >  6:72799/1‑149 (MQ=255)                   aGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCg                                                                                                                                   >  6:44770/1‑149 (MQ=255)                    gCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCgg                                                                                                                                  >  3:355152/1‑149 (MQ=255)                     cGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGc                                                                                                                                 >  7:188330/1‑149 (MQ=255)                       gtgtCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCt                                                                                                                               <  4:55729/149‑1 (MQ=255)                          tCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGAc                                                                                                                            >  2:385953/1‑149 (MQ=255)                          tCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGAc                                                                                                                            <  6:257395/149‑1 (MQ=255)                              tGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCt                                                                                                                         >  4:27342/1‑148 (MQ=255)                                gcaactgcaactGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCTAACTGCAGTTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTg                                                                                                                        <  8:62706/147‑1 (MQ=255)                                gcaactgcaactGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGa                                                                                                                       <  2:5458/148‑1 (MQ=255)                                     tgcaactgcGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAAc                                                                                                                 >  3:9414/1‑149 (MQ=255)                                       caactgcGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAAc                                                                                                                 <  2:54009/147‑1 (MQ=255)                                        aactgcGCCGCACGGGCGTGCGAGACCTGCGCCGTGGCGAACTGCATTTGGTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAACCCGGCCTGACCCTGTTCGAACTGa                                                                                                              <  4:214462/149‑1 (MQ=255)                                        aactgcGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGa                                                                                                              <  8:46511/149‑1 (MQ=255)                                          ctgcGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATg                                                                                                            <  1:385953/149‑1 (MQ=255)                                           tgcGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCTATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATgg                                                                                                           >  6:323769/1‑149 (MQ=255)                                                cgcACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCgcc                                                                                                      <  4:217838/149‑1 (MQ=255)                                                 gcACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACt                                                                                                                <  1:148721/138‑1 (MQ=255)                                                 gcACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACt                                                                                                                >  2:148721/1‑138 (MQ=255)                                                 gcACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCgccc                                                                                                     <  3:202396/149‑1 (MQ=255)                                                       gCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCTTGAAACACCg                                                                                                                                           <  3:55588/105‑1 (MQ=255)                                                       gCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCTTGAAACACCg                                                                                                                                           >  4:55588/1‑105 (MQ=255)                                                        cGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGACACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCgccctggcc                                                                                                <  1:177317/147‑1 (MQ=255)                                                        cGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCgccctggccct                                                                                              <  4:9414/149‑1 (MQ=255)                                                               gaCCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGcc                                                                                       >  3:132285/1‑149 (MQ=255)                                                                  cTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGCCCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGCCTGCCGtt                                                                                    >  7:224475/1‑149 (MQ=255)                                                                  cTGCGCCGTGGCGAACTGAAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGACTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGtt                                                                                    >  5:229463/1‑149 (MQ=255)                                                                   tGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTc                                                                                   >  8:247776/1‑149 (MQ=255)                                                                     cgcCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTc                                                                                   <  1:8061/147‑1 (MQ=255)                                                                     cgcCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCtt                                                                                 <  6:382795/149‑1 (MQ=255)                                                                      gcCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTg                                                                                <  5:44770/149‑1 (MQ=255)                                                                      gcCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTg                                                                                <  8:188330/149‑1 (MQ=255)                                                                         gTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGca                                                                             <  5:411638/149‑1 (MQ=255)                                                                          tGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGa                                                                                            <  5:82243/133‑1 (MQ=255)                                                                          tGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGa                                                                                            >  6:82243/1‑133 (MQ=255)                                                                            gCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGcatc                                                                           <  1:244085/148‑1 (MQ=255)                                                                                  tGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTcg                                                                    >  3:69378/1‑149 (MQ=255)                                                                                  tGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTAACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCtt                                                                                 >  2:51489/1‑136 (MQ=255)                                                                                  aattgcagTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTcg                                                                    <  8:231048/142‑1 (MQ=255)                                                                                               cGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTg                                                                                         >  8:307488/1‑115 (MQ=255)                                                                                               cGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTg                                                                                         <  7:307488/115‑1 (MQ=255)                                                                                                  ccGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAAtgg                                                    <  8:78702/149‑1 (MQ=255)                                                                                                   cgcgACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAAtggc                                                   >  7:34468/1‑149 (MQ=255)                                                                                                      gACCAGCGCCTGGCACTGGGCCAGTACTCCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGAGCCTGATCGAAGTGATGGTCGCCCTGTCCCTGACTGCGGTTCTTGGCATCATGCTCGCCGGGCTGGTCAAtggctgg                                                <  4:189036/149‑1 (MQ=255)                                                                                                      gACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAAtggctgg                                                <  6:229463/149‑1 (MQ=255)                                                                                                        ccAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATggctggct                                              >  7:259421/1‑149 (MQ=255)                                                                                                         cAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATggctggct                                              >  6:131364/1‑148 (MQ=255)                                                                                                               cTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGACCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTg                                       >  6:340085/1‑149 (MQ=255)                                                                                                                  ccACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCTTGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTcg                                                                    >  3:387511/1‑117 (MQ=255)                                                                                                                  ccACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCTTGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTcg                                                                    <  4:387511/117‑1 (MQ=255)                                                                                                                  ccACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTgcgc                                    <  4:132285/149‑1 (MQ=255)                                                                                                                  ccACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTgc                                      <  3:27342/147‑1 (MQ=255)                                                                                                                  ccACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGGCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTgc                                      <  8:224475/147‑1 (MQ=255)                                                                                                                   cACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTgcgc                                    >  3:307948/1‑148 (MQ=255)                                                                                                                   cACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTgcgc                                    <  6:324711/148‑1 (MQ=255)                                                                                                                   cACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTgcgc                                    >  5:324711/1‑148 (MQ=255)                                                                                                                   cACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTgcgc                                    >  1:301256/1‑148 (MQ=255)                                                                                                                        ggCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGc                              <  2:301256/149‑1 (MQ=255)                                                                                                                         gCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGcc                             <  4:355152/149‑1 (MQ=255)                                                                                                                          ccAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCt                            <  4:307948/149‑1 (MQ=255)                                                                                                                           cAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTg                           <  8:259421/149‑1 (MQ=255)                                                                                                                             gTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTGCa                         <  7:247776/149‑1 (MQ=255)                                                                                                                                                 gcCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTGCAGGACACCACTCGGCAAAtct     >  5:365292/1‑149 (MQ=255)                                                                                                                                                    cATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTGCAGGACACCACTCGGCAAATCTCgg  <  5:340085/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AGCTCGGCCAAGGTGGCCAGCGGTGTCACTGGCAACTGCAACTGCGCCGCACCGGCGTGCGAGACCTGCGCCGTGGCGAACTGCAATTGTTCGCCGACCGCGACCAGCGCCTGCCACTGGGCCAGTACACCGTGTTTCTGGAGCGCCCATGAAACACCGCCAAACCGGCCTGACCCTGATCGAACTGATGGTCGCCCTGGCCCTGACTGCCGTTCTTGGCATCATGCTCGCCGCGCTGGTCAATGGCTGGCTGAAGGTGCGCGAACGCCTGCAGGACACCACTCGGCAAATCTCGG  >  NC_002947/1201449‑1201744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑