Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,210,829 T→A 12.1% intergenic (+19/+18) ytnA → / ← PP_1060 amino acid permease YtnA/glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,210,8290TA12.1% 83.5 / 4.0 33intergenic (+19/+18)ytnA/PP_1060amino acid permease YtnA/glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base T (9/20);  new base A (2/2);  total (11/22)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01

GTACTTCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATCTGC  >  NC_002947/1210684‑1210973
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gTACTTCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTTACCTGACGTGGGGCCGCTTTGCg                                                                                                                                                 <  5:4219/149‑1 (MQ=255)
gTACTTCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCAGACCTGACGTGGGGCCGCttttag                                                                                                                                                 >  8:4627/1‑146 (MQ=255)
      cGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCAAAGCGGcccc                                                                                                                                            <  2:54805/148‑1 (MQ=255)
       gAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCAAAGCGGCCCCac                                                                                                                                          <  2:255515/149‑2 (MQ=255)
         ggATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCg                                                                                                                                        <  2:211840/149‑1 (MQ=255)
              cgcgTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTtctc                                                                                                                                   <  5:201331/149‑1 (MQ=255)
                 gtgtGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCa                                                                                                                                <  7:4627/149‑1 (MQ=255)
                 gtgtGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCa                                                                                                                                <  7:324850/149‑1 (MQ=255)
                 gtgtGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCa                                                                                                                                <  4:176388/149‑1 (MQ=255)
                     ggCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCGTTTCGGCCCCCGCGGTGTATCAGg‑cc                                                                                                                           >  6:206581/1‑149 (MQ=255)
                          tGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCATTTAGGCCCCACCGGTATATCAGG‑CCCCCa                                                                                                                       >  4:302132/1‑148 (MQ=255)
                                       ggCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGc                                                                                                         <  5:206581/149‑1 (MQ=255)
                                              cTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTc                                                                                                                   <  1:358764/132‑1 (MQ=255)
                                               tGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCGTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGCGTTTGGGCCCCCCCCGTATCTCAGG‑GCGCCCCATAGCTGTggggggcgcaa                                                                                                   >  2:27838/1‑140 (MQ=255)
                                                ggTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGCGTTGCGGCCCCCGCGTGATATCATG‑CCGCCACATAGCGGTGCGGCGCGaaaaat                                                                                                >  5:212078/1‑146 (MQ=255)
                                                 gtgtTGCTGACAGTGCTGTATTACACGTTCCGCGTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTg                                                                                                <  1:27838/148‑1 (MQ=255)
                                                      gCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑c                                                                                          <  3:302132/148‑1 (MQ=255)
                                                          aCAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCt                                                                                                        <  7:386847/131‑1 (MQ=255)
                                                            aGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTACGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGcc                                                                                   <  1:253524/149‑1 (MQ=255)
                                                                 tGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGGCGCGTTTAGGCGCCCGCGGTGTCTCAGG‑CCGCCACATAGCTGGGCGGGTCGAAACAGTCCGCCCCGGGcccttg                                                                               >  7:189426/1‑145 (MQ=255)
                                                                               cGCCTGGCGACGAAAGACTCACAATGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAAt                                                                <  6:283479/149‑1 (MQ=255)
                                                                                     gcgcCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACt                                                          <  3:364862/149‑1 (MQ=255)
                                                                                                   acaAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCATTTAGGCCCCAGCGGTGTCGAAGG‑CCCCCCCATAGCGGTGCGGGTCGAAACAGGACG‑CCCCGGCCCGGTGCCCCAAACGCGCAAAGAAAAGGGCGGTCAccaag                                            >  6:187424/1‑146 (MQ=255)
                                                                                                                        ctcctGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGc                       <  1:302883/149‑1 (MQ=255)
                                                                                                                        ctcctGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGc                       <  6:212078/149‑1 (MQ=255)
                                                                                                                           ctgacctgacGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCtt                     <  3:283221/148‑1 (MQ=255)
                                                                                                                           ctgacctgacGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTc                    <  1:162656/149‑1 (MQ=255)
                                                                                                                              acctgacGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCaa                  <  8:312257/148‑1 (MQ=255)
                                                                                                                                   acGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAacac            <  8:189426/149‑1 (MQ=255)
                                                                                                                                   acGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAaca             >  1:76483/1‑148 (MQ=255)
                                                                                                                                     gTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTACG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACAt          >  4:239167/1‑149 (MQ=255)
                                                                                                                                      tGGGGCCGCAAAGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATg         >  4:214164/1‑149 (MQ=255)
                                                                                                                                      tGGGGCCGCAAAGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATg         >  7:70985/1‑149 (MQ=255)
                                                                                                                                          gCCGCTTTGCGGCCCCAGCGTTCTCTCAGGACCGCCAATTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCAt      >  1:36367/1‑149 (MQ=255)
                                                                                                                                               tttGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATCTGc  >  4:167397/1‑147 (MQ=255)
                                                                                                                                               tttGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCA‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATCTGc  >  8:107121/1‑147 (MQ=255)
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GTACTTCGAGGATACGCGTGTGGCCCTGTACATCGGCCCGGCGTTCCTGGTGTTGCTGACAGTGCTGTATTACACGTTCCGCCTGGCGCCGAAAGACGCACAAGGTGTGACGAGTACAGCCTCCTGACCTGACGTGGGGCCGCTTTGCGGCCCCAGCGTTCTCTCAGG‑CCGCCACTTCGCTGTGCGGCTCGAAACTGTCCG‑CCCGGGCCAGTTGCCACATCCGCGAATAGAACTGGCCGTTCACCTCGCCGGTCAGCAGCTCACCTGGCTTCAAGAACACATGCATCTGC  >  NC_002947/1210684‑1210973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑