Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,212,380 T→A 7.5% E29D (GAA→GAT)  PP_1060 ← glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,212,3800TA 7.5% 149.3 / 2.7 53E29D (GAA→GAT) PP_1060glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base T (23/26);  new base A (2/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-01

GCGGATTTCCGGGCGAATGGCCTCGACCAGATAGCGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACCATA  >  NC_002947/1212233‑1212524
                                                                                                                                                    |                                                                                                                                                
gCGGATTTCCGGGCGAATGGCCTCGACCAGATAGCGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCAtt                                                                                                                                                 >  3:326285/1‑149 (MQ=255)
     tttCCGGGCGAATGGCCTCGACCAGATAGCGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCgtgg                                                                                                                                            >  7:173406/1‑149 (MQ=255)
        ccGGGCGTATGGCCTCGACCAGATAGCGGATGTTGCCGATAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCGTGTGGTCTGGGTGAACGGCCACAACCATTCgtggtg                                                                                                                                          <  7:331261/148‑1 (MQ=255)
        ccGGGCGAATGGCCTCGACCAGATAGCGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGtggtgg                                                                                                                                         >  6:261733/1‑149 (MQ=255)
         cGGGCGAATGGCCTCGACCAGATAGCGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGc                                                                                                                                        >  6:78124/1‑149 (MQ=255)
          gggCGAATGGCCTCGACCAGATAGCGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCt                                                                                                                                       >  8:43887/1‑149 (MQ=255)
            gCGAATGGCCTCGACCAGATAGCGGATGTTGCAGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTg                                                                                                                                      >  2:44118/1‑148 (MQ=255)
                aTGGCCTCGACCAGATAGCGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTAAGGGAGAAAGGCAACAACCATTCGTGGTGGCTGAAgtg                                                                                                                                 >  5:329418/1‑147 (MQ=255)
                        gACCAGATAGCGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTc                                                                                                                          <  7:376891/148‑1 (MQ=255)
                          ccAGATAGCGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGc                                                                                                                        >  7:193573/1‑148 (MQ=255)
                           cAGATAGCGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCa                                                                                                                      <  5:32701/149‑1 (MQ=255)
                             gATAGCGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAgg                                                                                                                    >  7:102431/1‑149 (MQ=255)
                             gATAGCGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAgg                                                                                                                    <  7:43887/149‑1 (MQ=255)
                               tAGCGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGt                                                                                                                  <  6:302247/149‑1 (MQ=255)
                                  cGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGaa                                                                                                               >  3:225135/1‑149 (MQ=255)
                                  cGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGaa                                                                                                               <  5:78124/149‑1 (MQ=255)
                                   ggATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGaa                                                                                                               >  1:43591/1‑148 (MQ=255)
                                    gATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGaa                                                                                                               >  1:409082/1‑147 (MQ=255)
                                     aTGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAg                                                                                                            <  4:340842/149‑1 (MQ=255)
                                          gCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGTCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGcaca                                                                                                        <  5:1258/148‑1 (MQ=255)
                                          gCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGcaca                                                                                                        <  3:235589/148‑1 (MQ=255)
                                           ccGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGcaca                                                                                                        >  3:278830/1‑147 (MQ=255)
                                             gagaATCGGGTAGTTGCGGCGTACGGCGTGGCGGCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCAATCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAg                                                                                                     >  1:254558/1‑149 (MQ=255)
                                                  tCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACCACGAATGGttgtgg                                                                                                                                         >  2:263481/1‑107 (MQ=255)
                                                  tCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACCACGAATGGttgtgg                                                                                                                                         <  1:263481/107‑1 (MQ=255)
                                                          ttGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAgg                                                                                        <  4:225135/148‑1 (MQ=255)
                                                                       cGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGGGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTgag                                                                           >  3:318906/1‑148 (MQ=255)
                                                                        gTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGaa                                                                         <  4:278830/149‑1 (MQ=255)
                                                                          ggC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGt                                                                       <  8:173406/149‑1 (MQ=255)
                                                                           gc‑gcTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGt                                                                       >  6:174514/1‑148 (MQ=255)
                                                                                  ctgcAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCAATCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGc                                                                <  2:254558/149‑1 (MQ=255)
                                                                                      agcagGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGaagaag                                                                                         <  4:208027/120‑1 (MQ=255)
                                                                                        cagGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATAc                                                          <  3:118450/149‑1 (MQ=255)
                                                                                           gTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAgg                                                       <  8:193573/149‑1 (MQ=255)
                                                                                           gTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAgg                                                       >  7:306832/1‑149 (MQ=255)
                                                                                                 cAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCtt                                                 <  4:326285/149‑1 (MQ=255)
                                                                                                   ggCCGATCAGGCTGAGAATGCCTGTGGTCAGCGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGc                                               <  1:169610/149‑1 (MQ=255)
                                                                                                    gCCGATCAGGCTGAGAATGCCTGTGGTCAAGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTc                                                    <  6:124043/143‑1 (MQ=255)
                                                                                                    gCCGATCAGGCTGAGAATGCCTGTGGTCAAGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTc                                                    >  5:124043/1‑143 (MQ=255)
                                                                                                        aTCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATgg                                          <  8:102431/149‑1 (MQ=255)
                                                                                                             gCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCgg                                     >  4:198506/1‑149 (MQ=255)
                                                                                                               tGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCCTACAGGGACCGTGCGATGGCGCggg                                    >  4:262297/1‑147 (MQ=255)
                                                                                                                 agaATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAg                                 >  6:156495/1‑149 (MQ=255)
                                                                                                                  gaATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGc                                                                                       >  7:106645/1‑94 (MQ=255)
                                                                                                                  gaATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGc                                                                                       <  8:106645/94‑1 (MQ=255)
                                                                                                                    aTGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCTCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGc                               <  1:44118/148‑1 (MQ=255)
                                                                                                                       ccTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGTGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAg                           <  5:174514/149‑1 (MQ=255)
                                                                                                                        cTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAATGCATAACGACTGAGaa                                                                         <  4:116682/102‑1 (MQ=255)
                                                                                                                        cTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGCCAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAgg                          >  6:37227/1‑149 (MQ=255)
                                                                                                                              tCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTgg                    >  4:364917/1‑149 (MQ=255)
                                                                                                                                           ccACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCGGGGCCTGGGGGCAAACCCCGa       <  4:318906/149‑1 (MQ=255)
                                                                                                                                            cacaACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGa       >  5:275868/1‑148 (MQ=255)
                                                                                                                                                acaaTTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCTGCGAAGAAGGCATAACGACTGAGAAGTGACAGTCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGATCATa  <  6:329418/146‑1 (MQ=255)
                                                                                                                                                aCCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGATCATa  <  2:409082/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GCGGATTTCCGGGCGAATGGCCTCGACCAGATAGCGGATGTTGCCGAGAATCGGGTAGTTGCGGCGTACGGCGTGGC‑GCTGCTGCAGCAGGTCGAACAGGCCGATCAGGCTGAGAATGCCTGTGGTCAGGGTGAACGGCCACAACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACCATA  >  NC_002947/1212233‑1212524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑