| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,275,087 | G→C | 15.2% | intergenic (‑284/+28) | PP_5462 ← / ← PP_5463 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,275,087 | 0 | G | C | 15.2% | 99.2 / 12.6 | 46 | intergenic (‑284/+28) | PP_5462/PP_5463 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (21/17); new base C (2/5); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.31e-01 | |||||||||||
CGAATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGTTC > NC_002947/1274942‑1275231 | cGAATGGGCATTTTGGTCTATCCGGCTGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACCTCGGATCCAATAAGGTGAGGGATAGAGCTAAATATAGGGGAAAATGTGGTAAAGGTGAAAGGACAATGAGCAAAGCCCGCCGATgtgc > 4:352181/1‑149 (MQ=255)cGAATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTATAGGTGAAAGGAGAATGAGCAAATCCCGCCGATgggc > 8:89093/1‑149 (MQ=255) ggCATTTTGATCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGAGAGAGCGGTAATGATGGGGGGGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCCATCGGCGGGCt < 7:205033/148‑1 (MQ=255) gCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCCATCGGCGGGCttt < 6:238999/149‑2 (MQ=255) aaTTTTGGTCTATCCGGCTGAGAGTGAATGTCGAAGCCTGCTTCCAGGTTACGTCGGCTCCAATAATGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCa < 6:343233/148‑1 (MQ=255) aTTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑g < 6:344428/149‑1 (MQ=255) ttttGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑gg < 1:63636/149‑1 (MQ=255) ttttGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCCATCGGCGGGCtttgct < 5:36003/149‑5 (MQ=255) tCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGGGCGGGATAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑gggggggg < 4:41946/149‑1 (MQ=255) aTCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTACAAAAAGGAGCGGGAGAGCGGTAATGAAGGGGGTGATGGTGGTTAAGGTGAAAGGAGAATGAGCAAAGACAGCCGATGGGCGGGCGTGA‑gggggggggc > 4:225434/1‑146 (MQ=255) tCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGGGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTGA‑GGGGGGGGCAg > 4:286854/1‑148 (MQ=255) gAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGGCAGAAGGGCTTTCGt > 7:172704/1‑148 (MQ=255) aTGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTa > 1:351723/1‑141 (MQ=255) aTGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTa < 2:351723/141‑1 (MQ=255) cgccaaGCCTGCTGCCCCGCTACGGCGGCCCCAATCACGGCCGCGAGAGCGGTAAGGCTGGGGGGGAGGGCGGTAAAGGGGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACg < 8:232208/145‑1 (MQ=255) gAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGGTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTc > 2:114307/1‑149 (MQ=255) cTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTtc > 7:160773/1‑149 (MQ=255) tGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGcgatc > 1:232056/1‑145 (MQ=255) tGCATCCCGTCTACGTCGGCTCCCATAACGTGCCGGAGACCGCTAATGATGGGGGTCATGGTGGTAAAGGTGAAAGGAGACTGAGCAAACCCCGCCGATGGGCGGGCGTCA‑GGGGGCGGCAGAAGGGCTATCGTACTACGGTc < 2:232056/143‑1 (MQ=255) gCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCAGCCGATGGGCGGGCTTGA‑GGGGGGGGCAGAAGGGCTATAGTACAACGGTCGCTtatc > 7:182978/1‑149 (MQ=255) cTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGGGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATTGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTAAGGTCGCTTCTCg > 5:41447/1‑149 (MQ=255) cttACGTCGGCTCCAATAAGGGGCGGGCGAGCGGTAATGATGGGGGGGCTGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCg < 8:160773/148‑1 (MQ=255) cGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAgg > 7:111379/1‑147 (MQ=255) ggCTCCAATAAGGTGCGGGTTAGCGGTTATGATGGGGGTGATGGTGGTAATGGTTAAAGTAGACTCACCAAACCCCGCGGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTa < 8:131941/148‑1 (MQ=255) ggCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGCTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGAAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAg > 5:124055/1‑149 (MQ=255) cAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAg > 8:59028/1‑148 (MQ=255) cAATAAGGTGCGGGAGAGCGGTAATGATAGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGc < 3:140386/149‑1 (MQ=255) gCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCAcccgcccg > 7:342537/1‑149 (MQ=255) cGGGAGAGCGGTAATGAGGGGGGTGATGGTGGTAAAGGTGACAGGAGAATGCGCAACGCCCGCCGAGGCGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCAcctgcccg < 6:41447/148‑1 (MQ=255) gCGGTAATCATGGGGCTGATGGTGGTAAAGGCGAACGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCActcctc < 8:111379/149‑1 (MQ=255) atgatgGGGGTGATGGTGGTAAAGGTGAAAGGACAATGAGCAAAGCCCGCCGATGGGCGGGCTTAA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCCCCCGACCGCACTCCTCATCAg > 7:107468/1‑148 (MQ=255) tgatgGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAgg > 8:316890/1‑148 (MQ=255) gATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCAcccgc < 7:143880/121‑1 (MQ=255) gATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCAcccgc > 8:143880/1‑121 (MQ=255) gATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACtt > 5:200249/1‑148 (MQ=255) aTGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCtca > 7:366342/1‑132 (MQ=255) aTGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCtca < 8:366342/132‑1 (MQ=255) gtggtCAAGGTGAAAGGATAACGACCAACGCCCGGCGCTGGGCGGGCGTCACGGGGCGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAg < 8:172704/149‑1 (MQ=255) cacaGGAGACTGAGCAAACCCCCCCGACCGGCGGGCTTGA‑GGGCGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCttt < 3:225434/146‑1 (MQ=255) gAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTg > 1:62763/1‑148 (MQ=255) cAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATc > 3:285546/1‑149 (MQ=255) gaaGCCCGCCCATCGGCGGGCTTTA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCa > 7:326104/2‑121 (MQ=255) gaaGCCCGCCCATCGGCGGGCTTTA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCa < 8:326104/120‑1 (MQ=255) aGCCCGCCCATCGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGAGTGCCTTTGGATAAGTTGTATCCAg > 8:61690/1‑149 (MQ=255) ctccgccgATGGGCGTGCTTCA‑GGGGGTGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGc < 3:44422/147‑1 (MQ=255) cgccgATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCacc > 4:44422/1‑144 (MQ=255) ccgATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGtt > 2:131847/1‑149 (MQ=255) cgATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTACAGCGAAAGGTAGGAAGCACCCGCCCTCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGTTc < 8:182978/149‑1 (MQ=255) | CGAATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCA‑GGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGTTC > NC_002947/1274942‑1275231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |