Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,287,868	C→G	5.9%	T417T (ACC→ACG)	PP_1125 →	putative Helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,287,868	0	C	G	5.9%	218.4 / 2.4	68	T417T (ACC→ACG)	PP_1125	putative Helicase
Reads supporting (aligned to +/- strand):  ref base C (32/32);  new base G (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GAAAAAGGGCTTTGCCCGCCTCAACGATTTGTTCACCCGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGTCAACGCCAGCCCCATCCTCGCCGCCGATA  >  NC_002947/1287727‑1288016                                                                                                                                              |                                                                                                                                                     gAAAAAGGGCTTTGCCCGCCTCAACGATTTGTTCACCCGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGcc                                                                                                                                                >  3:392479/1‑148 (MQ=255)     aaGGGCTTTGCCCGCCTCAACGATTTGTTCACCCGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAggg                                                                                                                                           >  7:219512/1‑149 (MQ=255)        ggCTTTGCCCGCCTCAACGATTTGTTCACCCGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGGCACGGGTGACCAggctgc                                                                                                                                        <  4:191778/149‑5 (MQ=255)             tGCCCGCCTCAACGATTTGTTCACCCGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTgg                                                                                                                                    <  7:270348/148‑1 (MQ=255)              gCCCGCCTCAACGATTTGTTCACCCGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTgg                                                                                                                                    >  4:374523/1‑147 (MQ=255)              gCCCGCCTCAACGATTTGTGCACCCGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTggg                                                                                                                                   <  4:391626/148‑1 (MQ=255)                       aaCGATTTGTTCACCCGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGAc                                                                                                                         >  2:217015/1‑149 (MQ=255)                            tttGTTCACCCGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCAGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCt                                                                                                                    >  5:17309/1‑149 (MQ=255)                                ttCACCCGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCAc                                                                                                                <  3:374523/149‑1 (MQ=255)                                   aCCCGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCAccgc                                                                                                             >  3:395159/1‑149 (MQ=255)                                   aCCCGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCAccgc                                                                                                             >  7:232514/1‑149 (MQ=255)                                    cccGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCAccgcc                                                                                                            <  4:392479/149‑1 (MQ=255)                                     ccGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCAccgcc                                                                                                            >  8:139292/1‑148 (MQ=255)                                      cGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGa                                                                                                          <  3:179557/149‑1 (MQ=255)                                       gCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGaa                                                                                                         <  7:139292/149‑1 (MQ=255)                                           ggCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGAcc                                                                                                     >  8:15867/1‑149 (MQ=255)                                               gACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCgg                                                                                                  >  4:200878/1‑148 (MQ=255)                                                 ccTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGaa                                                                                                <  4:363649/148‑1 (MQ=255)                                                  cTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAg                                                                                                        <  6:263313/139‑1 (MQ=255)                                                  cTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAg                                                                                                        >  5:263313/1‑139 (MQ=255)                                                  cTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGa                                                                                              >  7:159407/1‑149 (MQ=255)                                                     cTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACa                                                                                            <  4:369516/148‑1 (MQ=255)                                                      tCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAgc                                                                                          <  2:9089/149‑1 (MQ=255)                                                        aaggaaggCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGATCCGGAAGACAgc                                                                                          <  6:180530/147‑1 (MQ=255)                                                        aaggaaggCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGATCCGGAAGACAgc                                                                                          >  5:180530/1‑147 (MQ=255)                                                        aaggaaggCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAgccc                                                                                        >  3:91470/1‑149 (MQ=255)                                                        aaggaaggCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAgccc                                                                                        >  3:399305/1‑149 (MQ=255)                                                        aaggaaggCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAgccc                                                                                        >  1:57678/1‑149 (MQ=255)                                                              ggCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCAt                                                                                  <  8:232514/149‑1 (MQ=255)                                                                 aTGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGgc                                                                               <  1:168061/149‑1 (MQ=255)                                                                        gCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCgctgg                                                                        <  7:306845/149‑1 (MQ=255)                                                                               cAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCt                                                                 >  3:91743/1‑149 (MQ=255)                                                                                    tCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGc                                                             <  4:395159/148‑1 (MQ=255)                                                                                      ggCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCg                                                           <  1:132746/148‑1 (MQ=255)                                                                                       gCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGa                                                          >  3:167086/1‑148 (MQ=255)                                                                                        cATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGaaa                                                        <  7:15867/149‑1 (MQ=255)                                                                                             ccagccaTCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGgc                                                   >  3:19876/1‑149 (MQ=255)                                                                                                gccaTCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCGGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGgcgcg                                                <  4:91470/149‑1 (MQ=255)                                                                                                gccaTCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGgcgcg                                                <  4:399305/149‑1 (MQ=255)                                                                                                 ccaTCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGgcgcgc                                               >  7:326704/1‑149 (MQ=255)                                                                                                  caTCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACT‑GGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTg                                             >  2:198825/1‑149 (MQ=255)                                                                                                   aTCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTgg                                                              >  8:162824/1‑132 (MQ=255)                                                                                                   aTCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTgg                                                              <  7:162824/132‑1 (MQ=255)                                                                                                      aGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGtt                                           <  5:252272/148‑1 (MQ=255)                                                                                                      aGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTc                                          >  1:68686/1‑149 (MQ=255)                                                                                                      aGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTc                                          <  8:219512/149‑1 (MQ=255)                                                                                                       ggCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCg                                         <  4:167086/149‑1 (MQ=255)                                                                                                        gCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGa                                        >  4:285450/1‑149 (MQ=255)                                                                                                        gCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGa                                        <  6:17309/149‑1 (MQ=255)                                                                                                          cGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGgcgc                                                 <  6:274967/138‑1 (MQ=255)                                                                                                          cGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGgcgc                                                 >  5:274967/1‑138 (MQ=255)                                                                                                                gTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGaa                                 <  8:159407/148‑1 (MQ=255)                                                                                                                gTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGaa                                 <  5:81208/148‑1 (MQ=255)                                                                                                                   gTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGTc                              >  2:379976/1‑148 (MQ=255)                                                                                                                     aTCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTg                                                               >  8:382767/1‑113 (MQ=255)                                                                                                                     aTCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTg                                                               <  7:382767/113‑1 (MQ=255)                                                                                                                      tCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGTCAAc                           <  4:91743/148‑1 (MQ=255)                                                                                                                      tCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGTCAACg                          >  7:46774/1‑149 (MQ=255)                                                                                                                       ccGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGTCAACGc                         >  1:22071/1‑149 (MQ=255)                                                                                                                         gCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGTCAACGc                         >  7:365171/1‑147 (MQ=255)                                                                                                                               cGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGTCAACGCCAGcccc                  <  2:57678/148‑1 (MQ=255)                                                                                                                                     ccTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGTCAACGCCAGCCCCATCCTcg           >  1:119137/1‑149 (MQ=255)                                                                                                                                     ccTGGTCACCCGTGCCCAGGGCAACT‑GGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGTCAACGCCAGCCCCATCCTcgc          <  1:198825/149‑1 (MQ=255)                                                                                                                                     ccTGGGCACGGGTGACCAGGGCAACAGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATCGCGCGCAGGCTCACCTTGGCCGAAAGTGGCGCGCTGGTCGACATCGAAGTCCACGCCCGCCCCCGCCTcg           >  8:328276/1‑149 (MQ=255)                                                                                                                                      cTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGTCAACGCCAGCCCCATCCTcgc          <  8:46774/149‑1 (MQ=255)                                                                                                                                      cTGGGCACGGGTGACCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGt                               <  7:308379/128‑1 (MQ=255)                                                                                                                                      cTGGGCACGGGTGACCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGt                               >  8:308379/1‑128 (MQ=255)                                                                                                                                       tGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGTCAACGCCAGCCCCATCCTcgcc         >  5:391645/1‑149 (MQ=255)                                                                                                                                              ccGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGTCAACGCCAGCCCCATCCTCGCCGCCGata  >  4:247693/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                                     GAAAAAGGGCTTTGCCCGCCTCAACGATTTGTTCACCCGCCTGGCTGACCTGCTCAAGGAAGGCATGGACGGCGAGGTCAATATCGGCATCGCCAGCCATCAGGCCGAAGAGTGGTATCCGCTGTTCGGCAGCCTGGTCACCCGTGCCCAGGGCAACTGGGAGCTGTGGACGGCCTTCACCGCCGAAGACCCGGAAGACAGCCCGCCCATGGCGCGCTGGCTGACCTTGGCCGAAAGTGGCGCGCTGTTCGACATCGAAGTCAACGCCAGCCCCATCCTCGCCGCCGATA  >  NC_002947/1287727‑1288016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑