Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,293,255 T→G 5.1% A286A (GCT→GCG)  PP_1128 → OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,293,2550TG 5.1% 249.2 / 2.6 78A286A (GCT→GCG) PP_1128OmpA family protein
Reads supporting (aligned to +/- strand):  ref base T (37/37);  new base G (2/2);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

GCGCCGAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAG  >  NC_002947/1293107‑1293400
                                                                                                                                                    |                                                                                                                                                 
gcgcCGAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCt                                                                                                                                                   <  7:80307/149‑1 (MQ=255)
   ccGAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGc                                                                                                                                                 >  7:205972/1‑148 (MQ=255)
    cGAGGGCCCCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTCCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGCTGGCGGTGCAGGATGCCAAGGCACCAGCTGcc                                                                                                                                                >  4:185519/1‑148 (MQ=255)
     gAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGGCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCaa                                                                                                                                              <  2:84427/149‑1 (MQ=255)
          ccGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCg                                                                                                                                         <  2:239110/149‑1 (MQ=255)
             cgcCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCtg                                                                                                                                      <  4:346812/149‑1 (MQ=255)
                ccTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCtggtg                                                                                                                                   >  1:365846/1‑149 (MQ=255)
                 cTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGTCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCtggtgg                                                                                                                                  <  2:103317/149‑1 (MQ=255)
                 cTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCtggtgg                                                                                                                                  >  5:144631/1‑149 (MQ=255)
                 cTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCGGCCTTGGCAGCtggtgc                                                                                                                                  <  5:71992/149‑2 (MQ=255)
                    aaCCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGc                                                                                                                                 <  8:205972/147‑1 (MQ=255)
                    aaCCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGCCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCGGCCTTGGCAGCtggtgcctt                                                                                                                               <  1:321227/149‑5 (MQ=255)
                           gcgTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGcc                                                                                                                        <  1:347675/149‑1 (MQ=255)
                               ggAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCGGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTc                                                                                                                     >  3:366049/1‑148 (MQ=255)
                               ggAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTc                                                                                                                     >  8:210660/1‑148 (MQ=255)
                                gAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCaa                                                                                                                   <  7:210660/149‑1 (MQ=255)
                                      ttGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTgc                                                                                                             <  3:340379/149‑1 (MQ=255)
                                        gCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTCGTGGCAAACCTGCCGTCAAGGCTgcg                                                                                                            <  1:11889/148‑1 (MQ=255)
                                          tGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAAc                                                                                                                             <  7:327764/129‑1 (MQ=255)
                                          tGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAAc                                                                                                                             >  8:327764/1‑129 (MQ=255)
                                            aCCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCgaa                                                                                                       <  3:4981/149‑1 (MQ=255)
                                            aCCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCgaa                                                                                                       <  3:218108/149‑1 (MQ=255)
                                              cccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGTACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAgg                                                                                                                 >  2:51164/1‑137 (MQ=255)
                                              cccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGTACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAgg                                                                                                                 <  1:51164/137‑1 (MQ=255)
                                              cccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCgaag                                                                                                      >  7:185123/1‑148 (MQ=255)
                                              cccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCgaag                                                                                                      >  5:165279/1‑148 (MQ=255)
                                              cccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCgaag                                                                                                      >  3:46523/1‑148 (MQ=255)
                                                 gCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAAc                                                                                                                             >  4:167627/1‑122 (MQ=255)
                                                 gCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAAc                                                                                                                             <  3:167627/122‑1 (MQ=255)
                                                 gCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAAc                                                                                                  >  7:271799/1‑149 (MQ=255)
                                                 gCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAAc                                                                                                  <  7:374249/149‑1 (MQ=255)
                                                   aaaaCACCATTGTCGCGCTGCTGTCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCt                                                                                                <  8:251457/149‑1 (MQ=255)
                                                   aaaaCACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCt                                                                                                <  8:185123/149‑1 (MQ=255)
                                                     aaCACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTg                                                                                               >  4:161436/1‑148 (MQ=255)
                                                     aaCACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTg                                                                                               >  2:131688/1‑148 (MQ=255)
                                                       cacCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAg                                                                                            <  3:161436/149‑1 (MQ=255)
                                                         ccATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGc                                                                                           >  5:101871/1‑148 (MQ=255)
                                                          cATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGcc                                                                                          >  7:313420/1‑148 (MQ=255)
                                                            tGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAg                                                                                       <  2:34040/149‑1 (MQ=255)
                                                              gTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAgg                                                                                      <  7:274951/148‑1 (MQ=255)
                                                              gTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAgg                                                                                      <  8:271799/148‑1 (MQ=255)
                                                              gTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGc                                                                                     >  2:157626/1‑149 (MQ=255)
                                                                       ctgGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGc                                                                             <  2:284324/148‑1 (MQ=255)
                                                                             aaaTACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGaa                                                                      <  6:144631/149‑1 (MQ=255)
                                                                               aTACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGa                                                                    <  1:131688/149‑1 (MQ=255)
                                                                                 aCCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACt                                                                  >  4:48640/1‑149 (MQ=255)
                                                                                    agcagGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCa                                                               <  8:313420/149‑1 (MQ=255)
                                                                                      cagGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGc                                                             <  3:203401/149‑1 (MQ=255)
                                                                                       agGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGcc                                                            <  6:165279/149‑1 (MQ=255)
                                                                                         gccgccCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGcc                                                            >  2:277392/1‑147 (MQ=255)
                                                                                         gccgccCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCaa                                                          <  7:157374/149‑1 (MQ=255)
                                                                                            gccCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGa                                                        >  1:187533/1‑148 (MQ=255)
                                                                                            gccCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGa                                                        >  6:19031/1‑148 (MQ=255)
                                                                                              cccGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGaaa                                                      >  2:312367/1‑148 (MQ=255)
                                                                                                      ccGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTgcg                                             >  6:325457/1‑149 (MQ=255)
                                                                                                          cccGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCgg                                         >  5:173919/1‑149 (MQ=255)
                                                                                                          cccGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCgg                                         <  2:187533/149‑1 (MQ=255)
                                                                                                          cccGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCgg                                         >  8:374730/1‑149 (MQ=255)
                                                                                                                 tCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGcc                                  >  7:375001/1‑149 (MQ=255)
                                                                                                                   gATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTgcgc                                            >  3:45375/1‑137 (MQ=255)
                                                                                                                   gATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTgcgc                                            <  4:45375/137‑1 (MQ=255)
                                                                                                                        tggCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGTAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTgccgcc                           <  8:214741/149‑1 (MQ=255)
                                                                                                                        tggCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGTAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTgccgc                            >  7:214741/1‑148 (MQ=255)
                                                                                                                          gCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCaa                         <  5:19031/149‑1 (MQ=255)
                                                                                                                              tGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGaaa                     <  8:178662/149‑1 (MQ=255)
                                                                                                                              tGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGa                       >  2:19081/1‑147 (MQ=255)
                                                                                                                                cAGGATGCCAAGGCACGAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCTCCGGCCAAGCCTGCCGCCAAGAAAg                    <  7:78496/148‑1 (MQ=255)
                                                                                                                                cAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAg                    <  6:173919/148‑1 (MQ=255)
                                                                                                                                   gATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGc                >  8:219206/1‑149 (MQ=255)
                                                                                                                                     tGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCACAACGCCTGCGAAGAATGAAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCg              >  7:351821/1‑149 (MQ=255)
                                                                                                                                     tGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCg              >  3:399907/1‑149 (MQ=255)
                                                                                                                                       ccAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGtt            >  8:383266/1‑149 (MQ=255)
                                                                                                                                        cAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTg           >  5:18471/1‑149 (MQ=255)
                                                                                                                                             cACCAGCGGCCTTGGCAGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGAc         >  2:317985/1‑146 (MQ=255)
                                                                                                                                             cACCAGCGGCCTTGGCAGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCATGTCGCCAAGAAAGCTGCCGTAGAc         >  8:140830/1‑146 (MQ=255)
                                                                                                                                              aCCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAg      >  3:145769/1‑148 (MQ=255)
                                                                                                                                              aCCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGACTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGACGCCAAGAAAGCTTCCGTGGACacg      >  4:5631/1‑146 (MQ=255)
                                                                                                                                               ccAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGTCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGaa    <  3:5631/149‑1 (MQ=255)
                                                                                                                                                 aGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAg  <  3:48640/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCGCCGAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAG  >  NC_002947/1293107‑1293400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑