Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,293,264 C→A 5.1% A289A (GCC→GCA)  PP_1128 → OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,293,2640CA 5.1% 257.6 / 3.2 79A289A (GCC→GCA) PP_1128OmpA family protein
Reads supporting (aligned to +/- strand):  ref base C (36/39);  new base A (2/2);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.73e-01

CCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAA  >  NC_002947/1293117‑1293410
                                                                                                                                                   |                                                                                                                                                  
ccGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCg                                                                                                                                                   <  2:239110/149‑1 (MQ=255)
   cgcCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCtg                                                                                                                                                <  4:346812/149‑1 (MQ=255)
      ccTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCtggtg                                                                                                                                             >  1:365846/1‑149 (MQ=255)
       cTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGTCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCtggtgg                                                                                                                                            <  2:103317/149‑1 (MQ=255)
       cTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCtggtgg                                                                                                                                            >  5:144631/1‑149 (MQ=255)
       cTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCGGCCTTGGCAGCtggtgc                                                                                                                                            <  5:71992/149‑2 (MQ=255)
          aaCCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGc                                                                                                                                           <  8:205972/147‑1 (MQ=255)
          aaCCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGCCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCGGCCTTGGCAGCtggtgcctt                                                                                                                                         <  1:321227/149‑5 (MQ=255)
                 gcgTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGcc                                                                                                                                  <  1:347675/149‑1 (MQ=255)
                     ggAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCGGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTc                                                                                                                               >  3:366049/1‑148 (MQ=255)
                     ggAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTc                                                                                                                               >  8:210660/1‑148 (MQ=255)
                      gAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCaa                                                                                                                             <  7:210660/149‑1 (MQ=255)
                            ttGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTgc                                                                                                                       <  3:340379/149‑1 (MQ=255)
                              gCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTCGTGGCAAACCTGCCGTCAAGGCTgcg                                                                                                                      <  1:11889/148‑1 (MQ=255)
                                tGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAAc                                                                                                                                       <  7:327764/129‑1 (MQ=255)
                                tGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAAc                                                                                                                                       >  8:327764/1‑129 (MQ=255)
                                  aCCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCgaa                                                                                                                 <  3:218108/149‑1 (MQ=255)
                                  aCCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCgaa                                                                                                                 <  3:4981/149‑1 (MQ=255)
                                    cccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGTACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAgg                                                                                                                           <  1:51164/137‑1 (MQ=255)
                                    cccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGTACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAgg                                                                                                                           >  2:51164/1‑137 (MQ=255)
                                    cccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCgaag                                                                                                                >  3:46523/1‑148 (MQ=255)
                                    cccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCgaag                                                                                                                >  5:165279/1‑148 (MQ=255)
                                    cccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCgaag                                                                                                                >  7:185123/1‑148 (MQ=255)
                                       gCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAAc                                                                                                                                       <  3:167627/122‑1 (MQ=255)
                                       gCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAAc                                                                                                                                       >  4:167627/1‑122 (MQ=255)
                                       gCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAAc                                                                                                            <  7:374249/149‑1 (MQ=255)
                                       gCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAAc                                                                                                            >  7:271799/1‑149 (MQ=255)
                                         aaaaCACCATTGTCGCGCTGCTGTCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCt                                                                                                          <  8:251457/149‑1 (MQ=255)
                                         aaaaCACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCt                                                                                                          <  8:185123/149‑1 (MQ=255)
                                           aaCACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTg                                                                                                         >  4:161436/1‑148 (MQ=255)
                                           aaCACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTg                                                                                                         >  2:131688/1‑148 (MQ=255)
                                             cacCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAg                                                                                                      <  3:161436/149‑1 (MQ=255)
                                               ccATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGc                                                                                                     >  5:101871/1‑148 (MQ=255)
                                                cATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGcc                                                                                                    >  7:313420/1‑148 (MQ=255)
                                                  tGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAg                                                                                                 <  2:34040/149‑1 (MQ=255)
                                                    gTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAgg                                                                                                <  8:271799/148‑1 (MQ=255)
                                                    gTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAgg                                                                                                <  7:274951/148‑1 (MQ=255)
                                                    gTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGc                                                                                               >  2:157626/1‑149 (MQ=255)
                                                             ctgGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGc                                                                                       <  2:284324/148‑1 (MQ=255)
                                                                   aaaTACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGaa                                                                                <  6:144631/149‑1 (MQ=255)
                                                                     aTACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGa                                                                              <  1:131688/149‑1 (MQ=255)
                                                                       aCCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACt                                                                            >  4:48640/1‑149 (MQ=255)
                                                                          agcagGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCa                                                                         <  8:313420/149‑1 (MQ=255)
                                                                            cagGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGc                                                                       <  3:203401/149‑1 (MQ=255)
                                                                             agGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGcc                                                                      <  6:165279/149‑1 (MQ=255)
                                                                               gccgccCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGcc                                                                      >  2:277392/1‑147 (MQ=255)
                                                                               gccgccCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCaa                                                                    <  7:157374/149‑1 (MQ=255)
                                                                                  gccCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGa                                                                  >  6:19031/1‑148 (MQ=255)
                                                                                  gccCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGa                                                                  >  1:187533/1‑148 (MQ=255)
                                                                                    cccGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGaaa                                                                >  2:312367/1‑148 (MQ=255)
                                                                                            ccGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTgcg                                                       >  6:325457/1‑149 (MQ=255)
                                                                                                cccGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCgg                                                   >  5:173919/1‑149 (MQ=255)
                                                                                                cccGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCgg                                                   <  2:187533/149‑1 (MQ=255)
                                                                                                cccGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCgg                                                   >  8:374730/1‑149 (MQ=255)
                                                                                                       tCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGcc                                            >  7:375001/1‑149 (MQ=255)
                                                                                                         gATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTgcgc                                                      >  3:45375/1‑137 (MQ=255)
                                                                                                         gATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTgcgc                                                      <  4:45375/137‑1 (MQ=255)
                                                                                                              tggCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGTAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTgccgcc                                     <  8:214741/149‑1 (MQ=255)
                                                                                                              tggCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGTAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTgccgc                                      >  7:214741/1‑148 (MQ=255)
                                                                                                                gCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCaa                                   <  5:19031/149‑1 (MQ=255)
                                                                                                                    tGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGaaa                               <  8:178662/149‑1 (MQ=255)
                                                                                                                    tGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGa                                 >  2:19081/1‑147 (MQ=255)
                                                                                                                      cAGGATGCCAAGGCACGAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCTCCGGCCAAGCCTGCCGCCAAGAAAg                              <  7:78496/148‑1 (MQ=255)
                                                                                                                      cAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAg                              <  6:173919/148‑1 (MQ=255)
                                                                                                                         gATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGc                          >  8:219206/1‑149 (MQ=255)
                                                                                                                           tGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCACAACGCCTGCGAAGAATGAAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCg                        >  7:351821/1‑149 (MQ=255)
                                                                                                                           tGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCg                        >  3:399907/1‑149 (MQ=255)
                                                                                                                             ccAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGtt                      >  8:383266/1‑149 (MQ=255)
                                                                                                                              cAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTg                     >  5:18471/1‑149 (MQ=255)
                                                                                                                                   cACCAGCGGCCTTGGCAGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGAc                   >  2:317985/1‑146 (MQ=255)
                                                                                                                                   cACCAGCGGCCTTGGCAGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCATGTCGCCAAGAAAGCTGCCGTAGAc                   >  8:140830/1‑146 (MQ=255)
                                                                                                                                    aCCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAg                >  3:145769/1‑148 (MQ=255)
                                                                                                                                    aCCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGACTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGACGCCAAGAAAGCTTCCGTGGACacg                >  4:5631/1‑146 (MQ=255)
                                                                                                                                     ccAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGTCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGaa              <  3:5631/149‑1 (MQ=255)
                                                                                                                                       aGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAg            <  3:48640/149‑1 (MQ=255)
                                                                                                                                             cAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTg      <  1:312367/149‑1 (MQ=255)
                                                                                                                                              aaGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAg                              <  2:156112/124‑1 (MQ=255)
                                                                                                                                              aaGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAg                              >  1:156112/1‑124 (MQ=255)
                                                                                                                                                 gCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCaa  <  5:325457/149‑1 (MQ=255)
                                                                                                                                                   cGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCaa  >  6:362032/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACCAGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGCCAA  >  NC_002947/1293117‑1293410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑