Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,294,417	A→G	8.4%	C10R (TGC→CGC) ‡	PP_1130 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,294,417	0	A	G	8.4%	199.8 / 5.6	71	C10R (TGC→CGC) ‡	PP_1130	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/35);  new base G (2/4);  total (32/39)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCTTTACCACCA  >  NC_002947/1294269‑1294559                                                                                                                                                      |                                                                                                                                                gACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCa                                                                                                                                                 <  8:374085/149‑1 (MQ=255) gACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCa                                                                                                                                                 <  8:338005/149‑1 (MQ=255)      cGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTg                                                                                                                                            >  1:132589/1‑149 (MQ=255)         cAGGTCGCTGGCCATCCAGCGGCGCATGCGCACATACAACCACCAGTCGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCTGCCGGGCTGtgt                                                                                                                                         <  8:150440/149‑3 (MQ=255)         cAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAAGTACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTa                                                                                                                                          >  1:105513/1‑149 (MQ=255)         cAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAAGTACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTa                                                                                                                                          >  1:100171/1‑149 (MQ=255)         cAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCTGCCGGGCTGtgt                                                                                                                                         <  8:212757/149‑3 (MQ=255)              cgcgGTCCATCCCGCGGCGCATGCGCACATACAAGCCCCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTACTCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCTGCCGGGCTGtgtcgaag                                                                                                                                    <  4:15236/149‑8 (MQ=255)                  gTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGACCGGCAGGCTGTACCCCGGCtg                                                                                                                                 >  3:264692/1‑148 (MQ=255)                      atccaGCGGCGCATGCGCACATACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCtgaatg                                                                                                                             >  2:89042/1‑148 (MQ=255)                       tccaTCGGCGCATGCTCACATACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCtgaatgaa                                                                                                                           <  3:22845/149‑1 (MQ=255)                       tccaGCGGCGCATGCGCACATACAACACCCAGTGGAAC‑TAGAGTCCCTCAACGTTGGTGACCACGACGATGAAGTAATACATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCGGTAACCCGGCggaatgaa                                                                                                                           <  2:67866/149‑1 (MQ=255)                                gcATGCGCACATACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAAtttt                                                                                                                  <  2:217808/149‑1 (MQ=255)                                gcATGCGCACATACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAAttt                                                                                                                   >  3:283515/1‑148 (MQ=255)                                 cATGCGCACATACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTtta                                                                                                                 >  5:103125/1‑149 (MQ=255)                                 cATGCGCACATACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTtta                                                                                                                 >  5:107733/1‑149 (MQ=255)                                      gcACATACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACg                                                                                                            <  3:275151/149‑1 (MQ=255)                                       cacaTACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGt                                                                                                           >  4:192884/1‑149 (MQ=255)                                        acaTACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAACCAATTTTATTACGtt                                                                                                          >  6:32177/1‑149 (MQ=255)                                        acaTACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGtt                                                                                                          >  5:265256/1‑149 (MQ=255)                                         caTACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGttt                                                                                                         <  2:132589/149‑1 (MQ=255)                                           tACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGt                                                                                                       <  7:160317/149‑1 (MQ=255)                                              aaCCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑cc                                                                                                   >  1:56227/1‑149 (MQ=255)                                              aaCCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑cc                                                                                                   >  6:274559/1‑149 (MQ=255)                                              aaCCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑cc                                                                                                   >  7:79570/1‑149 (MQ=255)                                                cgcaccttGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGt                                                                                                 <  3:323824/142‑1 (MQ=255)                                                  actaGTGGAAA‑TACAGTCCCGCAACGGTGGTGATCACTACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGa                                                                                               <  4:264692/146‑1 (MQ=255)                                                    caGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACCACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGa                                                                                               >  1:264717/1‑147 (MQ=255)                                                          aaa‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCTTGACAGCCGt                                                                                        >  5:317739/1‑148 (MQ=255)                                                              tACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAAt                                                                                    <  3:256001/149‑1 (MQ=255)                                                                 actCCCGCAACGGTGGGGACCACGACGATGAAGTAATCCATTGCTATTCTTGGTCTCACTTTAAAGGCCTTCGACACATCCCGGCATGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTCTA‑CCGTGACAGCCGTCAAtgtg                                                                                 <  7:255125/147‑1 (MQ=255)                                                                 aGTCCCGCAACGGAGGTGACCACCACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGCCAGGCTGTACCCCGGCTGAAAGCAAACAATTTTATTACGTTTGTA‑CCGAGACAGCCCTCAAtgtc                                                                                 >  4:269375/1‑148 (MQ=255)                                                                     ccGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGc                                                                             <  2:100171/149‑1 (MQ=255)                                                                     ccGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGc                                                                             <  2:105513/149‑1 (MQ=255)                                                                        cAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTc                                                                          <  1:111737/149‑1 (MQ=255)                                                                           cGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTaa                                                                       <  5:150933/149‑1 (MQ=255)                                                                            gggggtgACCCCGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAAt                                                                      <  8:270919/146‑1 (MQ=255)                                                                                  gACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTaa                                                                       <  1:189737/142‑1 (MQ=255)                                                                                  gACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTaa                                                                       >  2:189737/1‑142 (MQ=255)                                                                                      acgacgATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGa                                                            <  3:192884/149‑1 (MQ=255)                                                                                           gaTGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGa                                                            >  1:153945/1‑144 (MQ=255)                                                                                           gaTGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGa                                                            <  2:153945/144‑1 (MQ=255)                                                                                             tGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATgg                                                     <  6:103125/149‑1 (MQ=255)                                                                                              gAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGt                                                                                        <  5:143688/113‑1 (MQ=255)                                                                                              gAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGt                                                                                        >  6:143688/1‑113 (MQ=255)                                                                                               aaGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATgg                                                     <  1:89042/148‑1 (MQ=255)                                                                                               aaGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGga                                                    <  4:283515/148‑1 (MQ=255)                                                                                                 gTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGAc                                                 <  6:265256/149‑1 (MQ=255)                                                                                                       cATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCt                                                              <  1:300226/130‑1 (MQ=255)                                                                                                       cATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCt                                                              >  2:300226/1‑130 (MQ=255)                                                                                                       cATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACACCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTAc                                            >  7:33513/1‑148 (MQ=255)                                                                                                            cTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGc                                      <  2:264717/149‑1 (MQ=255)                                                                                                               ttCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGt                                    <  6:107733/148‑1 (MQ=255)                                                                                                                  ttGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAAtgt                                                                                  <  8:308179/99‑1 (MQ=255)                                                                                                                  ttGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAAtgt                                                                                  >  7:308179/1‑99 (MQ=255)                                                                                                                     gTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGa                                                            >  6:205643/1‑118 (MQ=255)                                                                                                                     gTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGa                                                            <  5:205643/118‑1 (MQ=255)                                                                                                                      tctcACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGc                            >  8:384004/1‑149 (MQ=255)                                                                                                                         cACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTAt                                                       <  6:120757/119‑1 (MQ=255)                                                                                                                         cACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTAt                                                       >  5:120757/1‑119 (MQ=255)                                                                                                                           cTTGAAAGGCCTTCGACACTCCCAGGCAGGCTGTACCGCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACTGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACGCATGCGTAAATCCGCTTTGc                       <  7:181885/149‑1 (MQ=255)                                                                                                                              gAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCtg                     <  5:32177/148‑1 (MQ=255)                                                                                                                                          gtacaGCCTGCCGGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCTTTa        >  6:89739/3‑149 (MQ=255)                                                                                                                                          gtacaGCCTGCCGGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCTTTa        >  6:406796/3‑149 (MQ=255)                                                                                                                                            acaGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCtggtg                  <  6:301142/137‑1 (MQ=255)                                                                                                                                            acaGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCtggtg                  >  5:301142/1‑137 (MQ=255)                                                                                                                                             caGCCTGCCGGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCTTTacca     <  5:406796/149‑1 (MQ=255)                                                                                                                                             caGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCTTTacca     <  8:33513/149‑1 (MQ=255)                                                                                                                                                cccGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCAGCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGc                >  1:132288/1‑135 (MQ=255)                                                                                                                                                cccGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGc                <  2:132288/135‑1 (MQ=255)                                                                                                                                                cccGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCAGCTTTGCTGGTGGCGACCTTTacctcga  >  8:178490/1‑145 (MQ=255)                                                                                                                                                    gCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCTTTacc      <  1:13860/141‑1 (MQ=255)                                                                                                                                                    gCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCTTTacc      >  2:13860/1‑141 (MQ=255)                                                                                                                                                      |                                                                                                                                                GACAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAA‑TACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTA‑CCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCTTTACCACCA  >  NC_002947/1294269‑1294559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑