Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,320,300 T→A 9.4% T811S (ACA→TCA)  PP_1154 ← sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,320,3000TA 9.4% 208.8 / 9.1 74T811S (ACA→TCA) PP_1154sensory box protein
Reads supporting (aligned to +/- strand):  ref base T (34/33);  new base A (4/3);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

GCTGTGCAGTGTTTTGGGAGGGTTGGTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACGGGTAGGTACGCAGGTAGCCCAACGAC  >  NC_002947/1320153‑1320445
                                                                                                                                                    |                                                                                                                                                 
gCTGTGCAGTGTTTTGGGAGGGTTGGTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGctgtc                                                                                                                                                  <  7:16886/149‑1 (MQ=255)
       aGTGTTTTGGGAGGGTTGGTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGctgtcactgtca                                                                                                                                           <  3:387222/149‑1 (MQ=255)
         tgtTTTGGGAGGGTTGGTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGc                                                                                                                                         >  6:20382/1‑149 (MQ=255)
         tgtTTTGGGAGGGTTGGTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGc                                                                                                                                         >  6:26099/1‑149 (MQ=255)
           ttttGGGAGGGTTGGTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGccc                                                                                                                                       >  5:81317/1‑149 (MQ=255)
           ttttGGGAGGGTTGGTCCAACAACGCCGTCAACCCGGCGCTGTCCAGCGGCTTGCTCAG‑GTAATAGCATTGCAATACATGCCAGGGGTCATTTACAAGCCACTTGAGTTGATTTACGATTACCACGCATTCGGCTTTCACTGACAGcc                                                                                                                                        >  4:254552/1‑148 (MQ=255)
             ttGGGAGGGTTGGTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAGTGTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGccc                                                                                                                                       >  1:115743/1‑148 (MQ=255)
             ttGGGAGGGTTGGTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAGATTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGccc                                                                                                                                       >  1:110706/1‑148 (MQ=255)
                ggAGGGTTGGTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCTCGCCTTCGGCTGTCACTGTCAGCCCCAtcg                                                                                                                                  <  1:33095/149‑1 (MQ=255)
                 gAGGGTTGGTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCAtcgc                                                                                                                                 >  2:333433/1‑149 (MQ=255)
                   gggTTGGTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCAtcgcct                                                                                                                               <  6:191832/149‑1 (MQ=255)
                    ggTTGGTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCAtcgcctc                                                                                                                              >  8:197193/1‑149 (MQ=255)
                     gTTGGTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCAtcgcctcg                                                                                                                             >  7:278036/1‑149 (MQ=255)
                         gTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCAtcgc                                                                                                                                 >  5:189708/1‑141 (MQ=255)
                         gTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCAtcgc                                                                                                                                 <  6:189708/141‑1 (MQ=255)
                         gTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCa                                                                                                                         <  2:291581/149‑1 (MQ=255)
                         gTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCa                                                                                                                         <  3:341250/149‑1 (MQ=255)
                               caacGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTtga                                                                                                                   >  4:89162/1‑149 (MQ=255)
                               caacGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTtga                                                                                                                   >  2:79675/1‑149 (MQ=255)
                                aacGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTtgat                                                                                                                  >  7:73165/1‑149 (MQ=255)
                                 acGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTtgatg                                                                                                                 <  3:89162/149‑1 (MQ=255)
                                    ccGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGatgatg                                                                                                              <  5:207244/149‑1 (MQ=255)
                                    ccGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGACAGTGACAGccgaag                                                                                                                                    <  3:367638/127‑5 (MQ=255)
                                    ccGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGACAGTGACAGccgaag                                                                                                                                    >  4:367638/1‑123 (MQ=255)
                                      gTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGc                                                                                                            <  7:197193/149‑1 (MQ=255)
                                         aaCCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTg                                                                                                         <  7:153344/149‑1 (MQ=255)
                                            ccGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGAc                                                                                                      <  8:353418/149‑1 (MQ=255)
                                            ccGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTAGCCCAGGTTGATGATGGCCTGGAc                                                                                                      >  8:144282/1‑149 (MQ=255)
                                              ggCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACca                                                                                                    >  3:9235/1‑149 (MQ=255)
                                                       cAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTTGTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGt                                                                                           >  1:288818/1‑149 (MQ=255)
                                                       cAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGt                                                                                           >  5:278611/1‑149 (MQ=255)
                                                                gCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTgccgc                                                                                  >  3:401614/1‑149 (MQ=255)
                                                                    aG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGTTGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCgtt                                                                              <  1:333433/149‑1 (MQ=255)
                                                                    aG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCgtt                                                                              <  3:337858/149‑1 (MQ=255)
                                                                     g‑gTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCgttg                                                                             <  3:128271/149‑1 (MQ=255)
                                                                         aaTAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCgttgttg                                                                          >  3:30502/1‑149 (MQ=255)
                                                                          aTAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCgttgttgt                                                                         >  6:181893/1‑149 (MQ=255)
                                                                          aTAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCgttgttgt                                                                         >  7:112138/1‑149 (MQ=255)
                                                                          aTAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCgttgttg                                                                          <  5:170004/148‑1 (MQ=255)
                                                                             gCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCa                                                                      <  1:20967/149‑1 (MQ=255)
                                                                              ccTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAg                                                                     <  8:73165/149‑1 (MQ=255)
                                                                                     cTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCt                                                               >  6:66787/1‑148 (MQ=255)
                                                                                      ttCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCtga                                                             >  3:394647/1‑149 (MQ=255)
                                                                                        cATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCtga                                                             <  5:26099/147‑1 (MQ=255)
                                                                                        cATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCtga                                                             <  5:20382/147‑1 (MQ=255)
                                                                                          tGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGaa                                                         <  6:67883/149‑1 (MQ=255)
                                                                                          aggCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGaa                                                         >  7:348493/2‑149 (MQ=255)
                                                                                            gCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAg                                                        <  4:9235/148‑1 (MQ=255)
                                                                                                     cTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGa                                              >  7:17598/1‑149 (MQ=255)
                                                                                                      ttttCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACcaca                                                                                                  <  8:384729/96‑1 (MQ=255)
                                                                                                      ttttCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACcaca                                                                                                  >  7:384729/1‑96 (MQ=255)
                                                                                                        ttCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGAt                                             <  5:66787/147‑1 (MQ=255)
                                                                                                        ttCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATc                                            <  2:288818/148‑1 (MQ=255)
                                                                                                           cAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTg                                         >  2:241593/1‑148 (MQ=255)
                                                                                                           cAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTg                                         <  1:241593/148‑1 (MQ=255)
                                                                                                           cAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGa                                        <  4:192757/149‑1 (MQ=255)
                                                                                                                  ttGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTc                                 >  4:2845/1‑149 (MQ=255)
                                                                                                                    gAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTGGTCGATCTTGATGGTGTCg                                >  8:339696/1‑148 (MQ=255)
                                                                                                                      gTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAAc                             <  1:162548/149‑1 (MQ=255)
                                                                                                                       ttGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTGAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAAc                             <  8:348493/148‑1 (MQ=255)
                                                                                                                         gATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACggg                          >  4:329894/1‑149 (MQ=255)
                                                                                                                          aTGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACGggt                         <  4:401614/149‑1 (MQ=255)
                                                                                                                           tGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACGggta                        <  4:30502/149‑1 (MQ=255)
                                                                                                                           tGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACGggta                        <  6:278611/149‑1 (MQ=255)
                                                                                                                             ttCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACGggtagg                      >  2:230324/1‑149 (MQ=255)
                                                                                                                                    tCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACGGGTAGGTACGc                 <  2:115743/147‑1 (MQ=255)
                                                                                                                                    tCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACGGGTAGGTACGc                 <  2:110706/147‑1 (MQ=255)
                                                                                                                                     ccACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACGGGTAGGTACGCAgg              >  4:236416/1‑149 (MQ=255)
                                                                                                                                       aCGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTc                                 <  6:356099/128‑1 (MQ=255)
                                                                                                                                       aCGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTc                                 >  5:356099/1‑128 (MQ=255)
                                                                                                                                         cgatggggCTGACAGTGACAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACGGGTAGGTACGCAGGTAGc          >  6:154116/7‑149 (MQ=255)
                                                                                                                                           atggggCTGACAGTGACAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCgttgttg                                                                          >  1:238874/5‑83 (MQ=255)
                                                                                                                                           atggggCTGACAGTGACAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCgttgttg                                                                          <  2:238874/79‑1 (MQ=255)
                                                                                                                                             ggggCTGACAGTGACAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACg                            >  5:276179/3‑127 (MQ=255)
                                                                                                                                             ggggCTGACAGTGACAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACg                            <  6:276179/125‑1 (MQ=255)
                                                                                                                                                gCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACGGGTAGGTACGCAGGTAGCCCAacga   >  6:66714/1‑149 (MQ=255)
                                                                                                                                                 ctgtcactgtcaGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACGGGTAGGTACGCAGGTAGCCCAacgac  <  6:81317/149‑1 (MQ=255)
                                                                                                                                                 ctgtcactgtcaGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACGGGTAGGTACGCAGGTAGCCCAacgac  <  8:278036/149‑1 (MQ=255)
                                                                                                                                                  tgtcactgtcaGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACGGGTAGGTACGCAGGTAGCCCAacgac  >  2:151348/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCTGTGCAGTGTTTTGGGAGGGTTGGTGCAACAACGCCGTCAACCCGGCGCTGTCCAGGGGCTTGCTCAG‑GTAATAGCCTTGCACTTCATGGCAGCGGTCCTTTTCCAGCGACTTGAGTTGATGTTCGCTTTCCACGCCTTCGGCTGTCACTGTCAGCCCCATCGCCTCGCCCAGGTTGATGATGGCCTGGACCACAGCGCGGTCGCTGCCGCCGTTGTTGTTCAGGCCGCTGATGAAGCGCTTGTCGATCTTGATGCTGTCGAACGGGTAGGTACGCAGGTAGCCCAACGAC  >  NC_002947/1320153‑1320445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑