Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,342,251 C→T 11.5% intergenic (‑35/+20) PP_1166 ← / ← PP_1167 DMT superfamily permease/TRAP dicarboxylate transporter subunit DctM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,342,2510CT11.5% 136.0 / 7.4 53intergenic (‑35/+20)PP_1166/PP_1167DMT superfamily permease/TRAP dicarboxylate transporter subunit DctM
Reads supporting (aligned to +/- strand):  ref base C (18/28);  new base T (2/4);  total (20/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01

GGCGATGCGCGCGGCGATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCTTG  >  NC_002947/1342106‑1342394
                                                                                                                                                 |                                                                                                                                               
ggCGATGCGCGCAGCGATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAg                                                                                                                                              <  7:84885/149‑1 (MQ=255)
   gATGCGCGCGGCGATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCgg                                                                                                                                           >  8:62982/1‑149 (MQ=255)
    aTGCGCGCGGCGATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGc                                                                                                                                          <  7:286643/149‑1 (MQ=255)
     tGCGCGCGGCGATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGG‑AAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGccc                                                                                                                                        >  6:44408/1‑149 (MQ=255)
       cgcgcgGCGATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCTTGGCGGcccc                                                                                                                                       <  2:400657/149‑1 (MQ=255)
        gcgcgGCGATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGccccc                                                                                                                                      >  7:89295/1‑149 (MQ=255)
          gcggcgATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCGGGGGCCGCTTGGCGGCCCCTgg                                                                                                                                    <  4:284009/149‑1 (MQ=255)
          gcggcgATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCgg                                                                                                                                    <  3:226237/149‑1 (MQ=255)
           cggcgATGTGGTTGCCAGCGAATGAACAGGCCAGGCGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGt                                                                                                                                   <  8:15943/149‑1 (MQ=255)
               gATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATa                                                                                                                               <  4:177101/149‑1 (MQ=255)
                   tgGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTa                                                                                                                           <  6:347990/149‑1 (MQ=255)
                      ttGCCAGCGAAGGACCAGGCCAGGCGGGCGAGCAGGGCGATGGCTAGTTTGCGGGGGACACGCGGTCCAGCCGAGGAGGGAACAGCCAGGTGCAGGATCCCTTTGCAAGCGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAg                                                                                                                        <  6:392053/149‑1 (MQ=255)
                          cAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGcacc                                                                                                                    >  4:21752/1‑149 (MQ=255)
                           aGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGcacca                                                                                                                   >  2:385783/1‑149 (MQ=255)
                              gAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACg                                                                                                                 >  4:258326/1‑148 (MQ=255)
                              gAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGCGAAAAGCGGTACAGCCGAGGAGGTAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGCGGCCGCCACGCGCCCCCCGGTCAAAATTACAGCACCACg                                                                                                                 >  8:62345/1‑148 (MQ=255)
                                aTGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCCCCACGc                                                                                                                >  7:191526/1‑147 (MQ=255)
                                  gAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAgg                                                                                                            >  6:50344/1‑149 (MQ=255)
                                   aaCAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAgg                                                                                                            <  5:389459/148‑1 (MQ=255)
                                        gCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCTTGGCGGCCCCTggg                                                                                                                                   <  2:34226/120‑2 (MQ=255)
                                        gCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCTTGGCGGCCCCTggg                                                                                                                                   >  1:34226/1‑119 (MQ=255)
                                           aggaggGCGAGCAGGGCGATGGCTAGTTTGCGTGGG‑AAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGc                                                                                                  <  5:44408/149‑1 (MQ=255)
                                                          gCGATGGCTAGTTTGCGTGGGAAAAGCGCTACAGCCGAGGAGGGAACAGCCATGGGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAAt                                                                                    <  8:50106/149‑1 (MQ=255)
                                                              tGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTaa                                                                                <  7:238168/149‑1 (MQ=255)
                                                               ggCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGAGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAg                                                                               >  1:95408/1‑149 (MQ=255)
                                                                gCTAGTTTGCGTGGGAACAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAg                                                                               <  8:364889/148‑1 (MQ=255)
                                                                  tAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTg                                                                                        <  2:58974/137‑1 (MQ=255)
                                                                  tAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGAGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTg                                                                                        >  1:58974/1‑137 (MQ=255)
                                                                         cGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCAt                                                                     <  1:385783/149‑1 (MQ=255)
                                                                             ggAATATCGGGACATCCGAGGAGGGTACTGCCATGTTCAGGATCCATTTGCAAGAGCCCAGGGGACGCGAAGCGTCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTATGTCACTGCCATCAGc                                                                 <  4:381610/149‑1 (MQ=255)
                                                                                aaaGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCAcc                                                              <  3:258326/149‑1 (MQ=255)
                                                                                     ggTACAGCCTAGGAGGGAACTGCCATGTGCATGTTCCATTTGCAAGAGGCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAATTCACTGCCATCAGCACCATGaa                                                         <  7:62345/149‑1 (MQ=255)
                                                                                        aCAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCg                                                                                                                                     <  6:127551/70‑1 (MQ=255)
                                                                                        aCAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCg                                                                                                                                     >  5:127551/1‑70 (MQ=255)
                                                                                         cAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAgc                                                     <  8:89295/149‑1 (MQ=255)
                                                                                            ccGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGcc                                                  <  3:21752/149‑1 (MQ=255)
                                                                                             cGAGGGGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAgcgc                                                   >  4:118554/1‑147 (MQ=255)
                                                                                                        cAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGc                                      <  2:95408/149‑1 (MQ=255)
                                                                                                            cATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCa                                  <  7:27280/149‑1 (MQ=255)
                                                                                                                tgCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGaa                                                         <  7:197168/122‑1 (MQ=255)
                                                                                                                tgCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGaa                                                         >  8:197168/1‑122 (MQ=255)
                                                                                                                      aTCCATTTGCAAGAGCCCAGGGGCCGCCAAGAGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGGTCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCAc                        >  5:331272/1‑149 (MQ=255)
                                                                                                                         cATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGt                     >  6:169861/1‑149 (MQ=255)
                                                                                                                          aTTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAgc                                                     >  4:98490/1‑116 (MQ=255)
                                                                                                                          aTTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAgc                                                     <  3:98490/116‑1 (MQ=255)
                                                                                                                          aTTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTc                    <  3:118554/149‑1 (MQ=255)
                                                                                                                           tttGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTAACTGCCATCAGCACCAGGAACAGCCCCAGCTAGAACGGCATCAACGCCTTCACCGTc                    >  8:349553/1‑148 (MQ=255)
                                                                                                                                 agagCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGa             <  5:50344/149‑1 (MQ=255)
                                                                                                                                   agCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATa           <  3:130399/149‑1 (MQ=255)
                                                                                                                                      ccAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTc        <  7:349553/149‑1 (MQ=255)
                                                                                                                                      ccAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTc        <  6:151591/149‑1 (MQ=255)
                                                                                                                                       cgggggCCGCTTGGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGa                  <  8:159725/136‑1 (MQ=255)
                                                                                                                                       cgggggCCGCTTGGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGa                  >  7:159725/3‑138 (MQ=255)
                                                                                                                                            gccgccAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCTTg  <  7:62982/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGCGATGCGCGCGGCGATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCTTG  >  NC_002947/1342106‑1342394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑