Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,352,293 G→T 6.1% S22* (TCG→TAG) ‡ PP_1178 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,352,2930GT 6.1% 320.6 / 7.1 98S22* (TCG→TAG) ‡PP_1178hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (44/48);  new base T (4/2);  total (48/50)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

CATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGAG  >  NC_002947/1352147‑1352437
                                                                                                                                                  |                                                                                                                                                   
catcatATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAg                                                                                                                                                   >  3:91297/1‑149 (MQ=255)
caccccatGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCTCCGTGGGCTTGCCGGGGATGAGGCCAGCCAGCACTAGCTACGGTGGGTGTTCTGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAg                                                                                                                                                   <  1:145136/143‑1 (MQ=255)
           gtTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGcc                                                                                                                                      >  3:381216/1‑149 (MQ=255)
             ctCCTGTGGCTTGTGTTGGCGGAGTCGGCCTGACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCg                                                                                                                                     <  5:262931/147‑1 (MQ=255)
                cTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCAACCGAG‑GTGAAA‑caacgg                                                                                                                                     >  6:262931/1‑140 (MQ=255)
                   aGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCt                                                                                                                               <  6:360054/148‑1 (MQ=255)
                    ggCTTGTGTTGGCGGAGTCGGCCTCACGGTGGGCTGGCGGGGGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGc                                                                                                                             <  2:67755/149‑1 (MQ=255)
                    ggCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGc                                                                                                                             <  3:319066/149‑1 (MQ=255)
                         gtgtTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGtt                                                                                                                          <  3:373962/147‑1 (MQ=255)
                            ttGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGTGGCCAGCCACCACTTGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTTCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGtt                                                                                                                                  <  7:77199/136‑1 (MQ=255)
                            ttGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGa                                                                                                                      <  3:13630/148‑1 (MQ=255)
                              ggCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGAcc                                                                                                                    >  4:190565/1‑148 (MQ=255)
                                 ggAGTCGGCCTCACGGTGTGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTTGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATc                                                                                                                <  3:35031/149‑1 (MQ=255)
                                  gAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCt                                                                                                               <  6:340267/149‑1 (MQ=255)
                                     ctggCCTCATCGCCGGCAAGCCCACGGTGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGc                                                                                                            >  8:70369/3‑149 (MQ=255)
                                       ggCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCga                                                                                                          <  1:256992/149‑1 (MQ=255)
                                        gCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGcc                                                                                                                                      <  2:322072/120‑1 (MQ=255)
                                        gCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGcc                                                                                                                                      >  1:322072/1‑120 (MQ=255)
                                          cTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGAt                                                                                                       <  3:177023/149‑1 (MQ=255)
                                          cTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGAt                                                                                                       >  2:308702/1‑149 (MQ=255)
                                          cTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGAt                                                                                                       >  8:201097/1‑149 (MQ=255)
                                          cTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGAt                                                                                                       >  2:5209/1‑149 (MQ=255)
                                          cTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGAt                                                                                                       <  5:312069/149‑1 (MQ=255)
                                             aCCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGcc                                                                                                    <  6:152007/149‑1 (MQ=255)
                                              ccGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCa                                                                                                   <  6:264090/149‑1 (MQ=255)
                                              ccGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCa                                                                                                   <  2:358519/149‑1 (MQ=255)
                                                gTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAACCGGCCGGTTTCTGCGTTGCGACCAAt                                                                                                                 <  1:210548/134‑1 (MQ=255)
                                                gTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAAt                                                                                                                 >  2:210548/1‑133 (MQ=255)
                                                        gCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑c                                                                                          <  4:277424/147‑1 (MQ=255)
                                                        gCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑Ccg                                                                                        >  2:1582/1‑149 (MQ=255)
                                                         ccGGCGATGCGGCCAGCCACACCAAGCTACGGGGGGTGTACAGCGGTTCGACCCTGTGTGTCTTGGGGGGCTTGCGGGCCGGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAAGCTTGCGAGATGCCAGTTTTTT‑Ccgc                                                                                       <  6:397569/149‑1 (MQ=255)
                                                         ccGGCGATGAGGCCAGCCACCCCTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAC‑CGGCCGGGTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑Ccgc                                                                                       >  4:373405/1‑149 (MQ=255)
                                                                tGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATgg                                                                                <  4:381216/149‑1 (MQ=255)
                                                                    gccagccaCCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTACTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGt                                                                            >  4:362444/1‑149 (MQ=255)
                                                                      cagccaCCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTa                                                                           >  5:411674/1‑148 (MQ=255)
                                                                      cagccaCCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTa                                                                           >  1:193721/1‑148 (MQ=255)
                                                                      cagccaCCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTa                                                                           >  1:9939/1‑148 (MQ=255)
                                                                      cagccaCCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTAc                                                                          <  2:193721/149‑1 (MQ=255)
                                                                         ccaccaCTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATc                                                                       <  2:9939/149‑1 (MQ=255)
                                                                             cacTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCttt                                                                   >  7:186094/1‑149 (MQ=255)
                                                                              acTAGCTACGGTGGGTGTACATCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCtttt                                                                  <  1:1582/149‑1 (MQ=255)
                                                                              acTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCtttt                                                                  <  1:64498/149‑1 (MQ=255)
                                                                                  gCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCCGCGCCATGGGCGTACATCCtttttt                                                                <  1:5209/148‑1 (MQ=255)
                                                                                  gCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCtttttt                                                                <  1:308702/147‑1 (MQ=255)
                                                                                    tACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTga                                                              >  4:137604/1‑147 (MQ=255)
                                                                                    tACGGTGGGTGTACAGCGTTTCGACAAAGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTga                                                              >  1:11759/1‑147 (MQ=255)
                                                                                      cGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAgg                                                          >  3:248607/1‑149 (MQ=255)
                                                                                      cGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAgg                                                          <  4:248607/149‑1 (MQ=255)
                                                                                         tgggtgTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTcc                                                       <  3:190565/149‑1 (MQ=255)
                                                                                           ggtgTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCt                                                      <  3:137604/148‑1 (MQ=255)
                                                                                            gtgtACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGTTTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGTTCCTg                                                     >  1:110580/1‑149 (MQ=255)
                                                                                             tgtACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGtt                                                   <  7:8500/149‑1 (MQ=255)
                                                                                             tgtACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGt                                                    >  4:395729/1‑148 (MQ=255)
                                                                                                 cAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCttt                                               >  1:103532/1‑149 (MQ=255)
                                                                                                  aGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCttt                                               >  2:9646/1‑148 (MQ=255)
                                                                                                     gTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATc                                                                       >  8:354465/1‑121 (MQ=255)
                                                                                                     gTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATc                                                                       <  7:354465/121‑1 (MQ=255)
                                                                                                          gACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCt                                      <  3:362444/149‑1 (MQ=255)
                                                                                                           aCAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTg                                     >  6:346899/1‑149 (MQ=255)
                                                                                                           aCAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTg                                     >  6:129296/1‑149 (MQ=255)
                                                                                                            cAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGc                                    <  5:346899/149‑1 (MQ=255)
                                                                                                              aTGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGTCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGt                                  <  8:186094/149‑1 (MQ=255)
                                                                                                              aTGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGt                                  >  4:40351/1‑149 (MQ=255)
                                                                                                               tgtgtgTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGt                                  >  4:209899/1‑148 (MQ=255)
                                                                                                               tgtgtgTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTg                                 <  1:9646/149‑1 (MQ=255)
                                                                                                                  gtgtCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGaa                               >  8:40494/1‑148 (MQ=255)
                                                                                                                  gtgtCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGaa                               >  2:312838/1‑148 (MQ=255)
                                                                                                                  gtgtCGTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGAGAGATGCCAGTTTTTT‑CAGCGCCATGGTCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTgga                               >  2:2898/1‑146 (MQ=255)
                                                                                                                      cTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTg                          >  7:251364/1‑149 (MQ=255)
                                                                                                                       ttGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTgg                         <  7:201097/149‑1 (MQ=255)
                                                                                                                         ggggTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGc                                    <  5:262364/136‑1 (MQ=255)
                                                                                                                         ggggTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGc                                    >  6:262364/1‑136 (MQ=255)
                                                                                                                         ggggTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTggg                        <  3:209899/148‑1 (MQ=255)
                                                                                                                         ggggTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTggg                        >  8:10278/1‑148 (MQ=255)
                                                                                                                         ggggTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTggg                        <  6:411674/148‑1 (MQ=255)
                                                                                                                            gTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGaa                               >  5:126950/1‑138 (MQ=255)
                                                                                                                            gTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGaa                               <  6:126950/138‑1 (MQ=255)
                                                                                                                             tGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAg                   <  3:395729/149‑1 (MQ=255)
                                                                                                                               cTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAgg                  <  2:110580/148‑1 (MQ=255)
                                                                                                                               cTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAgg                  <  2:103532/148‑1 (MQ=255)
                                                                                                                                ttGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTCCCGAGCTGCGTGAACAGTGGGCAAAggg                 >  4:139567/1‑148 (MQ=255)
                                                                                                                                ttGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAggg                 >  5:378349/1‑148 (MQ=255)
                                                                                                                                  gCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGc                >  7:147819/1‑147 (MQ=255)
                                                                                                                                    gTGCCTGTTCCACCGCG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCATTCTTGCTAGCTTCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCtt                                                <  5:461/113‑1 (MQ=255)
                                                                                                                                    gTGCCTGTTCCAACGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGCCCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATACTTTTTTGAGAGGTCATGTTCtg                                                >  6:461/1‑112 (MQ=255)
                                                                                                                                     tGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGt           >  8:108231/1‑149 (MQ=255)
                                                                                                                                       cccgTTTCACCTCG‑GTGGAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACACAGCTGCGTGAACAGTCGGCAAAGGGCAAGCttt         >  7:90997/4‑149 (MQ=255)
                                                                                                                                        ccgTTTCACCTCG‑GTGGAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTAcc                                           <  2:63144/112‑1 (MQ=255)
                                                                                                                                        ccgTTTCACCTCG‑GTGGAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTAcc                                           >  1:63144/3‑114 (MQ=255)
                                                                                                                                        ccgTTTCACCTCG‑GTGGAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTgg                         <  7:372888/130‑1 (MQ=255)
                                                                                                                                        ccgTTTCACCTCG‑GTGGAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTgg                         >  8:372888/3‑132 (MQ=255)
                                                                                                                                        cTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTtc        >  2:175458/1‑149 (MQ=255)
                                                                                                                                         tGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCGAGTTTTTT‑CCGCGCCATTGGCGTTCATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGTACAGTGGGCAAAGGGCAAGGTTtct       <  3:73940/149‑1 (MQ=255)
                                                                                                                                         cgTTTCACCTCG‑GTGGAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTGCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTtct       >  2:28619/2‑149 (MQ=255)
                                                                                                                                           ttCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTtct       <  7:10278/147‑1 (MQ=255)
                                                                                                                                           ttCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTctct     >  4:165521/1‑149 (MQ=255)
                                                                                                                                              cACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGa   <  8:251364/148‑1 (MQ=255)
                                                                                                                                              cACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGAg  >  3:121817/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGAG  >  NC_002947/1352147‑1352437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑