BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,352,299	A→G	6.2%	F20S (TTC→TCC)	PP_1178 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,352,299	0	A	G	6.2%	287.6 / 6.0	97	F20S (TTC→TCC)	PP_1178	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (44/47); new base G (4/2); total (48/49)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 101 total aligned reads displayed.

GTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGAGGCGCGGATAC  >  NC_002947/1352158‑1352447

gtTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGcc                                                                                                                                                >  3:381216/1‑149 (MQ=255)

  ctCCTGTGGCTTGTGTTGGCGGAGTCGGCCTGACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCg                                                                                                                                               <  5:262931/147‑1 (MQ=255)

     cTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCAACCGAG‑GTGAAA‑caacgg                                                                                                                                               >  6:262931/1‑140 (MQ=255)

        aGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCt                                                                                                                                         <  6:360054/148‑1 (MQ=255)

         ggCTTGTGTTGGCGGAGTCGGCCTCACGGTGGGCTGGCGGGGGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGc                                                                                                                                       <  2:67755/149‑1 (MQ=255)

         ggCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGc                                                                                                                                       <  3:319066/149‑1 (MQ=255)

              gtgtTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGtt                                                                                                                                    <  3:373962/147‑1 (MQ=255)

                 ttGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGTGGCCAGCCACCACTTGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTTCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGtt                                                                                                                                            <  7:77199/136‑1 (MQ=255)

                 ttGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGa                                                                                                                                <  3:13630/148‑1 (MQ=255)

                   ggCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGAcc                                                                                                                              >  4:190565/1‑148 (MQ=255)

                      ggAGTCGGCCTCACGGTGTGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTTGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATc                                                                                                                          <  3:35031/149‑1 (MQ=255)

                       gAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCt                                                                                                                         <  6:340267/149‑1 (MQ=255)

                            ggCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCga                                                                                                                    <  1:256992/149‑1 (MQ=255)

                             gCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGcc                                                                                                                                                >  1:322072/1‑120 (MQ=255)

                             gCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGcc                                                                                                                                                <  2:322072/120‑1 (MQ=255)

                               cTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGAt                                                                                                                 <  5:312069/149‑1 (MQ=255)

                               cTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGAt                                                                                                                 >  2:5209/1‑149 (MQ=255)

                               cTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGAt                                                                                                                 >  2:308702/1‑149 (MQ=255)

                               cTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGAt                                                                                                                 >  8:201097/1‑149 (MQ=255)

                               cTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGAt                                                                                                                 <  3:177023/149‑1 (MQ=255)

                                  aCCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGcc                                                                                                              <  6:152007/149‑1 (MQ=255)

                                   ccGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCa                                                                                                             <  6:264090/149‑1 (MQ=255)

                                   ccGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCa                                                                                                             <  2:358519/149‑1 (MQ=255)

                                     gTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAACCGGCCGGTTTCTGCGTTGCGACCAAt                                                                                                                           <  1:210548/134‑1 (MQ=255)

                                     gTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAAt                                                                                                                           >  2:210548/1‑133 (MQ=255)

                                             gCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑c                                                                                                    <  4:277424/147‑1 (MQ=255)

                                             gCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑Ccg                                                                                                  >  2:1582/1‑149 (MQ=255)

                                              ccGGCGATGCGGCCAGCCACACCAAGCTACGGGGGGTGTACAGCGGTTCGACCCTGTGTGTCTTGGGGGGCTTGCGGGCCGGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAAGCTTGCGAGATGCCAGTTTTTT‑Ccgc                                                                                                 <  6:397569/149‑1 (MQ=255)

                                              ccGGCGATGAGGCCAGCCACCCCTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAC‑CGGCCGGGTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑Ccgc                                                                                                 >  4:373405/1‑149 (MQ=255)

                                                     tGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATgg                                                                                          <  4:381216/149‑1 (MQ=255)

                                                         gccagccaCCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTACTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGt                                                                                      >  4:362444/1‑149 (MQ=255)

                                                           cagccaCCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTa                                                                                     >  5:411674/1‑148 (MQ=255)

                                                           cagccaCCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTa                                                                                     >  1:9939/1‑148 (MQ=255)

                                                           cagccaCCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTa                                                                                     >  1:193721/1‑148 (MQ=255)

                                                           cagccaCCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTAc                                                                                    <  2:193721/149‑1 (MQ=255)

                                                              ccaccaCTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATc                                                                                 <  2:9939/149‑1 (MQ=255)

                                                                  cacTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCttt                                                                             >  7:186094/1‑149 (MQ=255)

                                                                   acTAGCTACGGTGGGTGTACATCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCtttt                                                                            <  1:1582/149‑1 (MQ=255)

                                                                   acTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCtttt                                                                            <  1:64498/149‑1 (MQ=255)

                                                                       gCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCCGCGCCATGGGCGTACATCCtttttt                                                                          <  1:5209/148‑1 (MQ=255)

                                                                       gCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCtttttt                                                                          <  1:308702/147‑1 (MQ=255)

                                                                         tACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTga                                                                        >  4:137604/1‑147 (MQ=255)

                                                                         tACGGTGGGTGTACAGCGTTTCGACAAAGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTga                                                                        >  1:11759/1‑147 (MQ=255)

                                                                           cGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAgg                                                                    >  3:248607/1‑149 (MQ=255)

                                                                           cGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAgg                                                                    <  4:248607/149‑1 (MQ=255)

                                                                              tgggtgTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTcc                                                                 <  3:190565/149‑1 (MQ=255)

                                                                                ggtgTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCt                                                                <  3:137604/148‑1 (MQ=255)

                                                                                 gtgtACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGTTTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGTTCCTg                                                               >  1:110580/1‑149 (MQ=255)

                                                                                  tgtACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGtt                                                             <  7:8500/149‑1 (MQ=255)

                                                                                  tgtACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGt                                                              >  4:395729/1‑148 (MQ=255)

                                                                                      cAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCttt                                                         >  1:103532/1‑149 (MQ=255)

                                                                                       aGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCttt                                                         >  2:9646/1‑148 (MQ=255)

                                                                                          gTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATc                                                                                 >  8:354465/1‑121 (MQ=255)

                                                                                          gTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATc                                                                                 <  7:354465/121‑1 (MQ=255)

                                                                                               gACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCt                                                <  3:362444/149‑1 (MQ=255)

                                                                                                aCAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTg                                               >  6:346899/1‑149 (MQ=255)

                                                                                                aCAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTg                                               >  6:129296/1‑149 (MQ=255)

                                                                                                 cAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGc                                              <  5:346899/149‑1 (MQ=255)

                                                                                                   aTGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGTCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGt                                            <  8:186094/149‑1 (MQ=255)

                                                                                                   aTGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGt                                            >  4:40351/1‑149 (MQ=255)

                                                                                                    tgtgtgTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGt                                            >  4:209899/1‑148 (MQ=255)

                                                                                                    tgtgtgTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTg                                           <  1:9646/149‑1 (MQ=255)

                                                                                                       gtgtCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGaa                                         >  8:40494/1‑148 (MQ=255)

                                                                                                       gtgtCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGaa                                         >  2:312838/1‑148 (MQ=255)

                                                                                                       gtgtCGTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGAGAGATGCCAGTTTTTT‑CAGCGCCATGGTCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTgga                                         >  2:2898/1‑146 (MQ=255)

                                                                                                           cTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTg                                    >  7:251364/1‑149 (MQ=255)

                                                                                                            ttGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTgg                                   <  7:201097/149‑1 (MQ=255)

                                                                                                              ggggTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGc                                              <  5:262364/136‑1 (MQ=255)

                                                                                                              ggggTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGc                                              >  6:262364/1‑136 (MQ=255)

                                                                                                              ggggTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTggg                                  >  8:10278/1‑148 (MQ=255)

                                                                                                              ggggTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTggg                                  <  3:209899/148‑1 (MQ=255)

                                                                                                              ggggTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTggg                                  <  6:411674/148‑1 (MQ=255)

                                                                                                                 gTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGaa                                         >  5:126950/1‑138 (MQ=255)

                                                                                                                 gTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGaa                                         <  6:126950/138‑1 (MQ=255)

                                                                                                                  tGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAg                             <  3:395729/149‑1 (MQ=255)

                                                                                                                    cTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAgg                            <  2:110580/148‑1 (MQ=255)

                                                                                                                    cTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAgg                            <  2:103532/148‑1 (MQ=255)

                                                                                                                     ttGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTCCCGAGCTGCGTGAACAGTGGGCAAAggg                           >  4:139567/1‑148 (MQ=255)

                                                                                                                     ttGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAggg                           >  5:378349/1‑148 (MQ=255)

                                                                                                                       gCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGc                          >  7:147819/1‑147 (MQ=255)

                                                                                                                         gTGCCTGTTCCACCGCG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCATTCTTGCTAGCTTCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCtt                                                          <  5:461/113‑1 (MQ=255)

                                                                                                                         gTGCCTGTTCCAACGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGCCCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATACTTTTTTGAGAGGTCATGTTCtg                                                          >  6:461/1‑112 (MQ=255)

                                                                                                                          tGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGt                     >  8:108231/1‑149 (MQ=255)

                                                                                                                            cccgTTTCACCTCG‑GTGGAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACACAGCTGCGTGAACAGTCGGCAAAGGGCAAGCttt                   >  7:90997/4‑149 (MQ=255)

                                                                                                                             ccgTTTCACCTCG‑GTGGAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTAcc                                                     <  2:63144/112‑1 (MQ=255)

                                                                                                                             ccgTTTCACCTCG‑GTGGAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTAcc                                                     >  1:63144/3‑114 (MQ=255)

                                                                                                                             ccgTTTCACCTCG‑GTGGAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTgg                                   <  7:372888/130‑1 (MQ=255)

                                                                                                                             ccgTTTCACCTCG‑GTGGAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTgg                                   >  8:372888/3‑132 (MQ=255)

                                                                                                                             cTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTtc                  >  2:175458/1‑149 (MQ=255)

                                                                                                                              tGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCGAGTTTTTT‑CCGCGCCATTGGCGTTCATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGTACAGTGGGCAAAGGGCAAGGTTtct                 <  3:73940/149‑1 (MQ=255)

                                                                                                                              cgTTTCACCTCG‑GTGGAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTGCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTtct                 >  2:28619/2‑149 (MQ=255)

                                                                                                                                ttCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTtct                 <  7:10278/147‑1 (MQ=255)

                                                                                                                                ttCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTctct               >  4:165521/1‑149 (MQ=255)

                                                                                                                                   cACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGa             <  8:251364/148‑1 (MQ=255)

                                                                                                                                   cACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGAg            >  3:121817/1‑149 (MQ=255)

                                                                                                                                        aG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGCGGcgcg       >  7:247150/1‑149 (MQ=255)

                                                                                                                                              aaa‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGAGGCGCGGATa   >  5:388831/1‑148 (MQ=255)

                                                                                                                                              aaa‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGAGGCGCGGATa   <  1:175458/148‑1 (MQ=255)

                                                                                                                                              aaa‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGAGGCGCGGATa   >  6:86485/1‑148 (MQ=255)

                                                                                                                                              aaa‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGAGGCGCGGATAc  <  3:40351/149‑1 (MQ=255)

GTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAG‑GTGAAA‑CGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTT‑CCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCTGAGGCGCGGATAC  >  NC_002947/1352158‑1352447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`