Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,384,161	C→A	13.4%	K290N (AAG→AAT)	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,384,161	0	C	A	13.4%	178.0 / 15.3	65	K290N (AAG→AAT)	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (26/30);  new base A (4/5);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

AGCGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCACGGTTTC  >  NC_002947/1384015‑1384307                                                                                                                                                   |                                                                                                                                                     aGCGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCtt                                                                                                                                                    <  1:374631/149‑1 (MQ=255)  gCGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTg                                                                                                                                                   <  6:228433/149‑1 (MQ=255)   cGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGc                                                                                                                                                  <  4:124431/149‑1 (MQ=255)    gggCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGcc                                                                                                                                                 <  2:146972/149‑1 (MQ=255)    gggCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGT‑GCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGccc                                                                                                                                                <  3:352558/149‑1 (MQ=255)      gCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCct                                                                                                                                               >  4:156085/1‑149 (MQ=255)      gCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCct                                                                                                                                               >  8:336163/1‑149 (MQ=255)       ccGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAG‑TCGTGGTCGCCACGGACGATCAGGGCAAGCTGATTGCCctga                                                                                                                                             <  8:207387/149‑3 (MQ=255)         gagcgagcCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAAGCTGATTGCCctgat                                                                                                                                            <  1:80411/149‑4 (MQ=255)          agcgagcCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAAGCTGATTGCCctgatc                                                                                                                                           <  6:311964/149‑5 (MQ=255)           gcgagcCGGCGCCGACGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCg                                                                                                                                          <  5:1970/149‑1 (MQ=255)            cgagcCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCg                                                                                                                                          <  3:253824/148‑1 (MQ=255)             gagcCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTg                                                                                                                                                   <  3:294238/138‑1 (MQ=255)                ccggcgccggcgCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCAcc                                                                                                                                     >  4:128522/1‑149 (MQ=255)                  ggcgccggcgccAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACcgc                                                                                                                                   <  8:19410/149‑1 (MQ=255)                             cAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGTACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCa                                                                                                                          <  8:158243/147‑1 (MQ=255)                               aTGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGt                                                                                                                      <  6:111488/149‑1 (MQ=255)                                    gTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGCCGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTgc                                                                                                                 >  5:361289/1‑148 (MQ=255)                                    gTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGa                                                                                                                 <  1:112783/149‑1 (MQ=255)                                    gTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGa                                                                                                                 >  4:153455/1‑149 (MQ=255)                                       gcgcAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACgg                                                                                                              >  6:131981/1‑149 (MQ=255)                                          cAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑Ct                                                                                                          >  8:375665/1‑149 (MQ=255)                                             ttCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGTT‑Ctttt                                                                                                       >  3:327515/1‑149 (MQ=255)                                             ttCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑Ctttt                                                                                                       <  4:398664/149‑1 (MQ=255)                                              tCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACGCGTTCGAGGTTGGTGGCCTTGATTTAGTTCAGTTCGTGGTCGCGAGGGACGATCAGGGCAATCATCTTGCTGTCTTCGGCACCGCTTACGATCAGGGTCTTGACGGT‑Ctttt                                                                                                       <  4:243522/148‑1 (MQ=255)                                                         ggCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGc                                                                                           >  6:141036/1‑149 (MQ=255)                                                            cATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGt                                                                                        >  4:334281/1‑149 (MQ=255)                                                            cATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCAACAGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGt                                                                                        >  7:76337/1‑149 (MQ=255)                                                             aTGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTg                                                                                       <  1:21356/149‑1 (MQ=255)                                                             aTGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTg                                                                                       <  7:375665/149‑1 (MQ=255)                                                                  cAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGtttt                                                                                  >  8:16879/1‑149 (MQ=255)                                                                  cAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGCCCGTGGtttt                                                                                  >  7:172235/1‑149 (MQ=255)                                                                           ggCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACt                                                                         >  2:41038/1‑149 (MQ=255)                                                                            gCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGACGTGGTTTTCCACCAACTg                                                                        >  3:392787/1‑149 (MQ=255)                                                                             cAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTgg                                                                       <  5:131981/149‑1 (MQ=255)                                                                                 cTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCaa                                                                   >  6:92659/1‑149 (MQ=255)                                                                                      ccAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑c                                                                                                           >  3:151999/1‑104 (MQ=255)                                                                                      ccAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑c                                                                                                           <  4:151999/104‑1 (MQ=255)                                                                                         gCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTg                                                           <  1:41038/149‑1 (MQ=255)                                                                                                  ccTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTg                                                                                                                  <  6:376303/86‑1 (MQ=255)                                                                                                  ccTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTg                                                                                                                  >  5:376303/1‑86 (MQ=255)                                                                                                  ccTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTg                                                                                                                  >  5:382394/1‑86 (MQ=255)                                                                                                  ccTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTg                                                                                                                  <  6:382394/86‑1 (MQ=255)                                                                                                  ccTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCggg                                                 <  3:153455/149‑1 (MQ=255)                                                                                                  ccTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCggg                                                 <  3:334281/149‑1 (MQ=255)                                                                                                   cTTGATTTCGTTCAGTTCGGGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGGGTACGATCAGGGGCTTGACGGTATTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCggg                                                 <  1:315203/149‑1 (MQ=255)                                                                                                    ttGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGgt                                               >  5:277715/1‑149 (MQ=255)                                                                                                      gATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGt                                                                                        >  7:219035/1‑107 (MQ=255)                                                                                                      gATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGt                                                                                        <  8:219035/107‑1 (MQ=255)                                                                                                         ttCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTCGa                                          >  4:14953/1‑149 (MQ=255)                                                                                                            gTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTCGACCa                                       <  3:128522/149‑1 (MQ=255)                                                                                                                 gTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTCGACCAGgcg                                   >  1:318642/1‑148 (MQ=255)                                                                                                                      tGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACAAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTCGACCAGGCGCAGctc                             >  1:327555/1‑149 (MQ=255)                                                                                                                           gCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTCGACCAGGCGCAGCTCCTCgg                        >  5:9498/1‑149 (MQ=255)                                                                                                                                ggACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAggg                   <  2:327555/149‑1 (MQ=255)                                                                                                                                    gaagaGGGCAAGCTGATTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCGACCAACAGGGCAATGGTCTTGG‑CATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAg               >  5:1326/5‑149 (MQ=255)                                                                                                                                    gaagaGGGCAAGCTGATTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCtt                                                            >  8:199407/5‑105 (MQ=255)                                                                                                                                    gaagaGGGCAAGCTGATTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCCATTCGGGGTGTCGACCAGGCGCAGc                               <  1:209974/130‑1 (MQ=255)                                                                                                                                    gaagaGGGCAAGCTGATTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTCGACCAGGCGCAGc                               >  2:209974/5‑133 (MQ=255)                                                                                                                                      agaGGGCAAGCTGATTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTc                                            >  4:383898/3‑118 (MQ=255)                                                                                                                                      agaGGGCAAGCTGATTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTc                                            <  3:383898/116‑1 (MQ=255)                                                                                                                                          ggCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCa         >  6:148466/1‑149 (MQ=255)                                                                                                                                           gCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTCGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCAc        <  5:92659/149‑1 (MQ=255)                                                                                                                                                 aGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCACGGTTTc  <  7:336163/149‑1 (MQ=255)                                                                                                                                                 aGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCACGGTTTc  <  3:156085/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     AGCGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGT‑CTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGG‑CATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCACGGTTTC  >  NC_002947/1384015‑1384307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑