Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,435,815 A→T 6.4% L50Q (CTG→CAG)  PP_1256 ← alpha‑ketoglutarate semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,435,8150AT 6.4% 228.6 / 4.2 78L50Q (CTG→CAG) PP_1256alpha‑ketoglutarate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (37/36);  new base T (2/3);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

GCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGG  >  NC_002947/1435674‑1435960
                                                                                                                                             |                                                                                                                                                 
gcCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAgcgcg                                                                                                                                              >  4:156033/1‑147 (MQ=255)
 ccTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAgcgcgc                                                                                                                                             >  8:109266/1‑147 (MQ=255)
 ccTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAgcgcgc                                                                                                                                             <  7:109266/147‑1 (MQ=255)
 ccTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAg                                                                                                                                           <  5:200414/149‑1 (MQ=255)
       gCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGACCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGAAGGCATCGAATGCCGCCCAGGCCTGCGCGCTGGCCTgg                                                                                                                                     <  5:308704/149‑1 (MQ=255)
           gCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCa                                                                                                                                 >  4:250066/1‑149 (MQ=255)
             cGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACg                                                                                                                               >  1:63381/1‑149 (MQ=255)
                tttCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACgtgt                                                                                                                            >  5:191733/1‑149 (MQ=255)
                    gATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCTAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCAc                                                                                                                                <  1:141623/141‑1 (MQ=255)
                    gATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCTAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCAc                                                                                                                                >  2:141623/1‑141 (MQ=255)
                    gATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGc                                                                                                                        >  1:162044/1‑149 (MQ=255)
                      tCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCt                                                                                                                      >  6:350468/1‑149 (MQ=255)
                        aCGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCtgt                                                                                                                    <  5:270324/149‑1 (MQ=255)
                         cGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCTGCCCAGGCCTGCGCGCTGGCCTGGGcggcat                                                                                                                              <  2:53711/138‑6 (MQ=255)
                         cGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCTGCCCAGGCCTGCGCGCTGGCCTGGGcggcat                                                                                                                              >  1:53711/1‑133 (MQ=255)
                           gcgcGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTgcc                                                                                                                 >  1:148296/1‑149 (MQ=255)
                               ggTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGccgccg                                                                                                             <  8:335383/149‑1 (MQ=255)
                                gTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGa                                                                                                            <  2:291701/149‑1 (MQ=255)
                                   gATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAgg                                                                                                          >  7:239905/1‑148 (MQ=255)
                                   gATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGt                                                                                                         <  2:148296/149‑1 (MQ=255)
                                     tCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGACATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAg                                                                                                       <  2:317759/149‑1 (MQ=255)
                                       aGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGc                                                                                                     >  5:345671/1‑149 (MQ=255)
                                         ggCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCt                                                                                                                      >  7:303687/1‑130 (MQ=255)
                                         ggCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCt                                                                                                                      <  8:303687/130‑1 (MQ=255)
                                              cGCCGAGGGCCTAGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGGGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTc                                                                                               >  3:257860/1‑148 (MQ=255)
                                                        cTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGAc                                                                                    >  1:340454/1‑149 (MQ=255)
                                                        cTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGAc                                                                                    >  4:274497/1‑149 (MQ=255)
                                                         tCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGATCGCCTGTGCCGCCCACGTAGGCCGGCTCCAACCACTGAcc                                                                                   >  8:309967/1‑149 (MQ=255)
                                                            aTCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTgg                                                                                                                                     <  4:89265/96‑1 (MQ=255)
                                                            aTCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTgg                                                                                                                                     >  3:89265/1‑96 (MQ=255)
                                                            aTCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGAc                                                                                    >  8:30389/1‑145 (MQ=255)
                                                            aTCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGAc                                                                                    <  7:30389/145‑1 (MQ=255)
                                                                 tgtgGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTg                                                                           >  2:392620/1‑149 (MQ=255)
                                                                     gCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGt                                                                        <  8:89758/148‑1 (MQ=255)
                                                                     gCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGt                                                                        <  2:340454/148‑1 (MQ=255)
                                                                     gCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGt                                                                        <  6:191733/148‑1 (MQ=255)
                                                                        aTGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGAt                                                                    <  2:293416/149‑1 (MQ=255)
                                                                          ggCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACCTCTGACCGGTGGTTGGGTCGATgg                                                                  >  5:287809/1‑149 (MQ=255)
                                                                            cTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCa                                                                <  2:196267/149‑1 (MQ=255)
                                                                             ttCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCAc                                                               >  7:375849/1‑149 (MQ=255)
                                                                             ttCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCAc                                                               >  1:350802/1‑149 (MQ=255)
                                                                               cAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACg                                                              >  1:295673/1‑148 (MQ=255)
                                                                                aaGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACgg                                                             <  6:287809/148‑1 (MQ=255)
                                                                                  ggAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATg                                                          <  3:250066/149‑1 (MQ=255)
                                                                                     aCTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGCTGGGTCGATGGCACGGATGGCg                                                       >  5:11236/1‑149 (MQ=255)
                                                                                              gttgttCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTg                                              >  3:13082/1‑149 (MQ=255)
                                                                                              gttgttCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTg                                              >  6:13466/1‑149 (MQ=255)
                                                                                               tagttCGAGCGATGTTTCGCGGAACGCATCGAATGCCGCCCATCCCAGCGCCCAGGCCTGGGCCACGTATTCGACTGTGACTCCGAGGTAGTCCGGCTACAAAGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGGGGCTGc                                             >  6:131398/3‑149 (MQ=255)
                                                                                                  ttCGCGCGATGTTTCGCGGTAGGCATCGAATGCGGCCCAGTCCAGCGCGCAGGCCTGGGCCACGTGTTCTCCGGTGCCGCCGAGGGAGGCCGGATCCAACGTCTGACCGGTGTTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCg                                           <  5:131398/148‑1 (MQ=255)
                                                                                                  ttCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCg                                           <  8:239905/148‑1 (MQ=255)
                                                                                                     gAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTa                                       >  4:86236/1‑149 (MQ=255)
                                                                                                       gCGTTGTTTCGCGTGAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGGCGCGGCTGCCGGTTAcc                                     <  4:257860/149‑1 (MQ=255)
                                                                                                       gCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGCTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTAcc                                     <  6:11236/149‑1 (MQ=255)
                                                                                                             tttCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCctc                               >  3:371993/1‑149 (MQ=255)
                                                                                                             tttCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCctc                               >  4:353260/1‑149 (MQ=255)
                                                                                                              ttCGCGGTAGGCATCGAATTCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGc                                  <  2:248430/145‑1 (MQ=255)
                                                                                                              ttCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGt                                         >  6:365063/1‑138 (MQ=255)
                                                                                                              ttCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGt                                         <  5:365063/138‑1 (MQ=255)
                                                                                                              ttCGCGGTAGGCATCGAATCCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGc                                  >  1:248430/1‑145 (MQ=255)
                                                                                                                 gcgGTAGGCATCGAAGGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTgg                            <  1:392620/148‑1 (MQ=255)
                                                                                                                         cATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagc                   <  5:152869/149‑1 (MQ=255)
                                                                                                                          aTCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTAc                                      <  6:51660/129‑1 (MQ=255)
                                                                                                                          aTCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTAc                                      >  5:51660/1‑129 (MQ=255)
                                                                                                                          aTCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCTGCCTCTg                             <  1:161169/138‑1 (MQ=255)
                                                                                                                          aTCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGAt                       <  8:45403/144‑1 (MQ=255)
                                                                                                                          aTCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGAt                       >  7:45403/1‑144 (MQ=255)
                                                                                                                          aTCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagca                  <  2:295673/149‑1 (MQ=255)
                                                                                                                           tCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagcag                 <  2:63381/149‑1 (MQ=255)
                                                                                                                           tCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagcag                 >  6:34845/1‑149 (MQ=255)
                                                                                                                                tGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGc            <  3:156033/149‑1 (MQ=255)
                                                                                                                                  ccgccCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCt          <  3:353260/149‑1 (MQ=255)
                                                                                                                                   cgccCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCtg         <  3:274497/149‑1 (MQ=255)
                                                                                                                                    gccCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTCCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCtgt        <  5:350468/149‑1 (MQ=255)
                                                                                                                                      ccAGGCCTGCTCGCTGGCCTGGGCCACGTGTTCGCCTGTGACGCAGAGGTAGGCCGGCTCCAACGTCTGACCGCTGGTTGGGTCCATg                                                                   >  2:65262/1‑88 (MQ=255)
                                                                                                                                      ccAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATg                                                                   <  1:65262/88‑1 (MQ=255)
                                                                                                                                      ccAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGGAGGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGTTCAGCAGGTGGCCtgt        >  8:141127/1‑147 (MQ=255)
                                                                                                                                      ccAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGa      <  6:345671/149‑1 (MQ=255)
                                                                                                                                          gCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAgggg  >  5:24752/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
GCCTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATCGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGG  >  NC_002947/1435674‑1435960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑