Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,453,671 C→T 7.1% A102V (GCG→GTG)  PP_1272 → multidrug MFS transporter membrane fusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,453,6710CT 7.1% 166.4 / 2.3 56A102V (GCG→GTG) PP_1272multidrug MFS transporter membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base C (27/25);  new base T (2/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

CTATGTGACCGCCGACTACACCGTGGTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGCCGACCATGAAGTCAACCGCTAC  >  NC_002947/1453525‑1453819
                                                                                                                                                  |                                                                                                                                                    
cTATGTGACCGCCGACTACACCGTGGTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTgcg                                                                                                                                                     >  6:407554/1‑148 (MQ=255)
   tgtgACCGCCGACTACACCGTGGTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTTGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTgcgca                                                                                                                                                   <  8:217010/147‑1 (MQ=255)
       aCCGCCGACTACACCGTGGTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTgcgcaggcg                                                                                                                                               >  7:303099/1‑147 (MQ=255)
         cgccgACTACACCGTGGTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGt                                                                                                                                           >  8:28738/1‑149 (MQ=255)
            cgACTACACCGTGGTCGAGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCATCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGc                                                                                                                                        <  1:148516/149‑1 (MQ=255)
              aCTACACCGTGGTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTg                                                                                                                                      <  1:221324/149‑1 (MQ=255)
              aCTACACCGTGGTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTg                                                                                                                                      <  6:197447/149‑1 (MQ=255)
               cTACACCGTGGTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTgg                                                                                                                                     <  3:284725/149‑1 (MQ=255)
               cTACACCGTGGTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTgg                                                                                                                                     <  5:416588/149‑1 (MQ=255)
                   acCGTGGTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGc                                                                                                                                 >  7:218493/1‑149 (MQ=255)
                      gTGGTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGcc                                                                                                                                >  6:36311/1‑147 (MQ=255)
                      gTGGTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCa                                                                                                                              <  5:13845/149‑1 (MQ=255)
                         gTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAgg                                                                                                                            <  7:28738/148‑1 (MQ=255)
                         gTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCGGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAgg                                                                                                                            <  5:407554/148‑1 (MQ=255)
                            gcgcCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGcac                                                                                                                         >  7:112872/1‑148 (MQ=255)
                              gcCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTgcgc                                                                                                                                                    >  3:14354/1‑119 (MQ=255)
                              gcCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTgcgc                                                                                                                                                    <  4:14354/119‑1 (MQ=255)
                               cccAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAg                                                                                                                     >  4:384300/1‑149 (MQ=255)
                               cccAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAg                                                                                                                     >  6:120634/1‑149 (MQ=255)
                                 cAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAgcg                                                                                                                   >  2:49719/1‑149 (MQ=255)
                                      tCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGAt                                                                                                              <  3:14974/149‑1 (MQ=255)
                                          cGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGAAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGccc                                                                                                          >  4:233221/1‑149 (MQ=255)
                                                  tcaAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACg                                                                                                  >  4:393609/1‑149 (MQ=255)
                                                     agcaagTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCtt                                                                                               >  1:396589/1‑149 (MQ=255)
                                                             cTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCa                                                                                       <  8:218493/149‑1 (MQ=255)
                                                               ggtggAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAgg                                                                                                                            >  8:187558/1‑110 (MQ=255)
                                                               ggtggAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAgg                                                                                                                            <  7:187558/110‑1 (MQ=255)
                                                                           tCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTg                                                                         >  1:310719/1‑149 (MQ=255)
                                                                           tCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTg                                                                         >  5:119593/1‑149 (MQ=255)
                                                                           tCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAAGACGCGCTGATTg                                                                         <  5:120634/149‑1 (MQ=255)
                                                                             agcagGTCACAGCTGGCCAGTTGCTGGCAACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGcc                                                                       >  3:225831/1‑149 (MQ=255)
                                                                              gcagGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGcc                                                                       >  5:78999/1‑148 (MQ=255)
                                                                               cagGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCa                                                                     <  8:112872/149‑1 (MQ=255)
                                                                                     acaGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCt                                                                              >  6:290631/1‑134 (MQ=255)
                                                                                     acaGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCt                                                                              <  5:290631/134‑1 (MQ=255)
                                                                                     acaGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGa                                                               <  6:88254/149‑1 (MQ=255)
                                                                                       aGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAg                                                             >  8:68202/1‑149 (MQ=255)
                                                                                        gCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGc                                                            <  6:78999/149‑1 (MQ=255)
                                                                                            gCCAGTTGCTGGCGACCATCGACGCGAGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCgg                                                          >  1:216448/1‑147 (MQ=255)
                                                                                               aGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGt                                                      >  7:179451/1‑148 (MQ=255)
                                                                                                   gCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAg                                                  <  2:396589/148‑1 (MQ=255)
                                                                                                         gACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGcc                                            >  1:140543/1‑148 (MQ=255)
                                                                                                           ccATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAg                                         <  4:225831/149‑1 (MQ=255)
                                                                                                            cATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAgg                                        <  7:68202/149‑1 (MQ=255)
                                                                                                            cATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAgg                                        >  4:143359/1‑149 (MQ=255)
                                                                                                                     gcgTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAgg                                                 >  6:18233/1‑131 (MQ=255)
                                                                                                                     gcgTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAgg                                                 <  5:18233/131‑1 (MQ=255)
                                                                                                                     gcgTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTgg                               <  2:140543/149‑1 (MQ=255)
                                                                                                                       gTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCg                             <  6:119593/149‑1 (MQ=255)
                                                                                                                            tACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGc                        >  5:133786/1‑149 (MQ=255)
                                                                                                                             aCCAGGCCGCGCTGGATGCTGTGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGcc                       >  4:263679/1‑149 (MQ=255)
                                                                                                                             aCCAGGCCGCGCTGGATGCTGTGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGcc                       >  4:258392/1‑149 (MQ=255)
                                                                                                                              ccAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGcc                       <  3:393609/148‑1 (MQ=255)
                                                                                                                               cAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGCCGa                     >  7:169778/1‑149 (MQ=255)
                                                                                                                                aGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGCCGAc                    >  2:84342/1‑149 (MQ=255)
                                                                                                                                 ggCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGCCGAcc                   <  3:384300/149‑1 (MQ=255)
                                                                                                                                 ggCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGCCGAcc                   <  2:310719/149‑1 (MQ=255)
                                                                                                                                    cgcgCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGCCGACCAt                 >  4:200813/1‑148 (MQ=255)
                                                                                                                                         tGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGCCGACCATGAAGt            >  4:249184/1‑148 (MQ=255)
                                                                                                                                            aTGCTGTGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGCCGACCATGAAGTCAAc        <  3:258392/149‑1 (MQ=255)
                                                                                                                                            aTGCTGTGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGCCGACCATGAAGTCAAc        <  3:263679/149‑1 (MQ=255)
                                                                                                                                              gctgcGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTg                                >  7:355377/1‑123 (MQ=255)
                                                                                                                                              gctgcGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTg                                <  8:355377/123‑1 (MQ=255)
                                                                                                                                              gctgcGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGCCGACCATGAAGTCAACCg      >  2:292209/1‑149 (MQ=255)
                                                                                                                                                  cgcaggcgcagTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGCCGACCATGAAGTCAACCGCTa   >  8:210208/1‑148 (MQ=255)
                                                                                                                                                  cgcaggcgcagTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGCCGACCATGAAGTCAACCGCTAc  <  3:200813/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CTATGTGACCGCCGACTACACCGTGGTCGCGCCCAAAGTCGCCGGTTTCATCAAGCAAGTGCTGGTGGAAGACAATCAGCAGGTCACAGCTGGCCAGTTGCTGGCGACCATCGACGCGCGTGACTACCAGGCCGCGCTGGATGCTGCGCAGGCGCAGTTGCTGGTAGCCCAGGCACAAAGCGCCGATGCCCGCGCCACGCTTGAACGCCAGGACGCGCTGATTGCCCAGGCCGAAGCGGCAGTGAAGGCGGCCCAGGCTGAGGTGGCGTTCGCCGACCATGAAGTCAACCGCTAC  >  NC_002947/1453525‑1453819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑