Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,457,917 T→G 8.6% V92V (GTT→GTG)  PP_1276 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,457,9170TG 8.6% 166.1 / 5.6 56V92V (GTT→GTG) PP_1276hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/23);  new base G (3/2);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACGGGAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTG  >  NC_002947/1457774‑1458059
                                                                                                                                               |                                                                                                                                              
tACGGGAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGAGAACCGGGCGCTTGTTcac                                                                                                                                             >  5:16107/1‑147 (MQ=255)
   gggAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAgcg                                                                                                                                        >  2:122976/1‑149 (MQ=255)
          ccGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTGAACAAgcgcccggtt                                                                                                                                 <  1:292298/149‑7 (MQ=255)
             aaaaaTCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAAcc                                                                                                                              <  6:16107/149‑1 (MQ=255)
                 aTCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTGAACAAgcgcccg                                                                                                                                    <  1:261095/139‑4 (MQ=255)
                 aTCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTGAACAAgcgcccg                                                                                                                                    >  2:261095/1‑136 (MQ=255)
                             gTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCg                                                                                                               >  5:47192/1‑148 (MQ=255)
                                 gcgTACAAGGGGCAGGCCGAAGATGCGGACGTCTAGATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGATGCGAACCGGGCGCTTGTTCTCAAGCGCGGCACAACCTATGGCGGCt                                                                                                                    <  5:130197/139‑1 (MQ=255)
                                 gcgTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATc                                                                                                          >  3:294450/1‑149 (MQ=255)
                                 gcgTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTGCACAAGCGCGGCACAACCTATGGCGGCt                                                                                                                    >  6:130197/1‑139 (MQ=255)
                                     aCAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATcc                                                                                                         <  4:151507/146‑1 (MQ=255)
                                     aCAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATcc                                                                                                         >  3:151507/1‑146 (MQ=255)
                                      cAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGc                                                                                                     <  6:47192/149‑1 (MQ=255)
                                      cAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGc                                                                                                     <  2:126048/149‑1 (MQ=255)
                                      cAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGc                                                                                                     >  3:395855/1‑149 (MQ=255)
                                           ggCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATc                                                                                                <  1:122976/149‑1 (MQ=255)
                                            gCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATcc                                                                                               <  8:240248/149‑1 (MQ=255)
                                                gCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGa                                                                                            >  7:155865/1‑148 (MQ=255)
                                                                  tACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTTAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCca                                                                          >  6:185386/1‑148 (MQ=255)
                                                                      ttGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAg                                                                      >  4:236311/1‑148 (MQ=255)
                                                                          cTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCAt                                                                   >  3:154342/1‑147 (MQ=255)
                                                                           ttCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAg                                                                >  1:138207/1‑149 (MQ=255)
                                                                                        tACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGCCTGAATCCTTc                                                   >  7:68635/1‑149 (MQ=255)
                                                                                         aCAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGaaa                                                                                          <  1:345533/109‑1 (MQ=255)
                                                                                         aCAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGaaa                                                                                          >  2:345533/1‑109 (MQ=255)
                                                                                          cAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCg                                                  >  4:1502/1‑148 (MQ=255)
                                                                                                ggCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTGGAAATGAATGCCACAAGGATCAGGGGTGAATCCTTCGTAGTTg                                            >  8:191437/1‑148 (MQ=255)
                                                                                                ggCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTg                                            <  4:294450/148‑1 (MQ=255)
                                                                                                ggCGTGCGGGATGTCGTCAACGGGCGTGAGGCGAACCCCGCGCTTGTTCACAACCGCGGCACAACCTATGGCGGCTATTCGAAATCCGGGAGTATCCGCAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCCAATTTg                                            >  5:177850/1‑148 (MQ=255)
                                                                                                 gCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGaa                                          <  3:236311/149‑1 (MQ=255)
                                                                                                   gTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAgg                                        >  4:117002/1‑149 (MQ=255)
                                                                                                   gTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGAACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAgg                                        <  2:26451/149‑1 (MQ=255)
                                                                                                     gCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGaa                                          >  8:11448/1‑145 (MQ=255)
                                                                                                     gCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGaa                                          <  7:11448/145‑1 (MQ=255)
                                                                                                      cGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAAc                                                                                                                               >  7:2412/1‑59 (MQ=255)
                                                                                                      cGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAAc                                                                                                                               <  8:2412/59‑1 (MQ=255)
                                                                                                       gggATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGggag                                    <  7:126454/149‑1 (MQ=255)
                                                                                                        gtaTGTCGTCATCGGGGGTGAGGCGTACAGGGGGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACTAGCATCAGGGCTGAATGCTTCGAATTTGAAGGggagg                                   <  6:177850/147‑1 (MQ=255)
                                                                                                        ggATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGggag                                    >  6:358581/1‑148 (MQ=255)
                                                                                                         gATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGggagga                                  >  3:135563/1‑149 (MQ=255)
                                                                                                         gATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGggagga                                  >  4:92776/1‑149 (MQ=255)
                                                                                                             tcgtcATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGgatga                               <  2:138207/148‑1 (MQ=255)
                                                                                                              cgtcATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAACCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGAtt                             >  5:353684/1‑149 (MQ=255)
                                                                                                                 cATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTg                           >  5:293845/1‑148 (MQ=255)
                                                                                                                    cGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGc                       >  8:68094/1‑149 (MQ=255)
                                                                                                                       gtgTGAGGCGAACCTGGCGCTTGTTCACAAGCGCTGCACAACCTATTGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGAc                    <  8:68635/147‑1 (MQ=255)
                                                                                                                            aGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCttt                >  4:21585/1‑148 (MQ=255)
                                                                                                                             ggCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCttt                >  7:316030/1‑147 (MQ=255)
                                                                                                                             ggCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCa              <  7:336209/149‑1 (MQ=255)
                                                                                                                             ggCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCa              <  3:117002/149‑1 (MQ=255)
                                                                                                                             ggCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGAGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCa              <  5:185386/149‑1 (MQ=255)
                                                                                                                              gCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCa              <  4:395855/148‑1 (MQ=255)
                                                                                                                                 aaCCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGggt          <  8:316030/149‑1 (MQ=255)
                                                                                                                                    cGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGggtggt       <  3:92776/149‑1 (MQ=255)
                                                                                                                                       gcgcTTGTGAACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTggtggg    >  5:105297/1‑149 (MQ=255)
                                                                                                                                         gcTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTggtgggtg  >  4:199804/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                              
TACGGGAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTG  >  NC_002947/1457774‑1458059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑