Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,505,544	A→C	5.4%	D219A (GAC→GCC)	petB →	cytochrome b

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,505,544	0	A	C	5.4%	230.5 / 3.6	74	D219A (GAC→GCC)	petB	cytochrome b
Reads supporting (aligned to +/- strand):  ref base A (34/36);  new base C (2/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

TTCGTGGTGACTACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATGGGCGGT  >  NC_002947/1505397‑1505689                                                                                                                                                    |                                                                                                                                                  ttCGTGGTGAGTACCTGATCTCGGGCCTGAGCCTGAACCGCTTCTACGCCCTGCATGTGGTTGCCCTGCCAATCGTGAGGGTCGGCCTGGTGGTATTGCACATCCTCGCCTGGAGCGAAGTGGGGTCGCGCAACCCGGATGGCGTCGa                                                                                                                                                   >  8:239383/1‑148 (MQ=255)  tCGTGGTGACTACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACa                                                                                                                                                 >  3:377764/1‑149 (MQ=255)       gtgACTACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCaa                                                                                                                                             <  4:238686/148‑1 (MQ=255)            tACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACGTCAagaaga                                                                                                                                        >  1:393914/1‑148 (MQ=255)            tACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAagaaga                                                                                                                                        >  6:118133/1‑148 (MQ=255)              ccTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGaaa                                                                                                                                      >  5:273262/1‑148 (MQ=255)               cTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGaaaaa                                                                                                                                    <  2:364814/149‑1 (MQ=255)               cTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGaaa                                                                                                                                      >  3:315142/1‑147 (MQ=255)                tGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCagg                                                                                                                                            <  1:102157/140‑3 (MQ=255)                tGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCagg                                                                                                                                            >  2:102157/1‑138 (MQ=255)                  aTCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAgg                                                                                                                                  <  4:315142/148‑1 (MQ=255)                   tctcGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGaaaaggac                                                                                                                                 <  2:255015/148‑5 (MQ=255)                       gggCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGaaa                                                                                                                            <  1:384456/149‑1 (MQ=255)                         gCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAAc                                                                                                                          >  1:393568/1‑149 (MQ=255)                         gCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAAc                                                                                                                          <  5:16608/149‑1 (MQ=255)                                ccTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTc                                                                                                                   >  3:272175/1‑149 (MQ=255)                                      ccGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATAGTGATTCTCGGCATGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGTCGACATCAAGAAGAAAAAGGACGAAAACGTTATTAAGCTg                                                                                                              >  7:251379/1‑148 (MQ=255)                                       cGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGa                                                                                                            <  4:377764/149‑1 (MQ=255)                                                 cccTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCg                                                                                                  >  3:402864/1‑149 (MQ=255)                                                  ccTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCag                                                                                                                                             >  1:386170/1‑104 (MQ=255)                                                  ccTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCag                                                                                                                                             <  2:386170/105‑2 (MQ=255)                                                   cTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGtt                                                                                                >  1:380861/1‑149 (MQ=255)                                                      gaTGTTGGTGCCGTGCCAATCTTTATTCTGGGCCTGGTTGTATTGCACATCCTCGGCTTGCACGAAGTGGGTTCTAACAACCCTGATGTCGTCGACATCAAGAAGAAAAAGGAGGAAAACGGTTTTCCGCTGGATGGTATTCCGTTc                                                                                               <  8:251379/146‑1 (MQ=255)                                                       aTGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGGATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCAc                                                                                            <  8:363310/149‑1 (MQ=255)                                                            gTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGt                                                                                        <  4:272175/148‑1 (MQ=255)                                                             ttGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGta                                                                                       <  1:336630/148‑1 (MQ=255)                                                             ttGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACAAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGtac                                                                                      >  2:106454/1‑149 (MQ=255)                                                                 ccTGCCAATCTTGCTTCTGGGCATGGTGGTATTGCAGATCCTCGCCTTGCTCGAATTGGGTTCGAACAACGCTGATGGCGTCGACATCAAGAAGAAAAAGGACTAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGtactac                                                                                   <  6:273262/148‑1 (MQ=255)                                                                  cTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTacac                                                                                 <  1:295518/149‑1 (MQ=255)                                                                     ccAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACGTCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACAcc                                                                                <  2:393914/147‑1 (MQ=255)                                                                      cAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGtacta                                                                                    >  4:246194/1‑142 (MQ=255)                                                                      cAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGtacta                                                                                    <  3:246194/142‑1 (MQ=255)                                                                         tCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTaaa                                                                          <  2:146365/149‑1 (MQ=255)                                                                            tGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATg                                                                                                          >  8:301684/1‑114 (MQ=255)                                                                            tGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATg                                                                                                          <  7:301684/114‑1 (MQ=255)                                                                             gATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACa                                                                      >  4:160441/1‑149 (MQ=255)                                                                              aTTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACa                                                                      <  5:297151/148‑1 (MQ=255)                                                                                  tcGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTc                                                                 >  3:57777/1‑149 (MQ=255)                                                                                      ccTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCg                                                             >  2:364628/1‑149 (MQ=255)                                                                                         ggtggtATTGCACATCCTCGCCTTTCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCgtgg                                                          <  2:393568/149‑1 (MQ=255)                                                                                          gtggtATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGtt                                                                             >  8:384822/1‑129 (MQ=255)                                                                                          gtggtATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGtt                                                                             <  7:384822/129‑1 (MQ=255)                                                                                          gtggtATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCgtggt                                                         >  2:353722/1‑149 (MQ=255)                                                                                           tggtATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCgtggtg                                                        <  2:100767/149‑1 (MQ=255)                                                                                               aTTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCTTGGTGgtgt                                                    >  6:52469/1‑149 (MQ=255)                                                                                                       tCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACGCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTc                                            <  4:402864/149‑1 (MQ=255)                                                                                                          tCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCgtggtg                                                        >  3:298541/1‑134 (MQ=255)                                                                                                          tCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCgtggtg                                                        <  4:298541/134‑1 (MQ=255)                                                                                                          tCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCgtg                                         >  1:384757/1‑149 (MQ=255)                                                                                                              cTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCt                                     <  3:16605/149‑1 (MQ=255)                                                                                                               ttGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTg                                    >  6:162320/1‑149 (MQ=255)                                                                                                                 gCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTgcg                                  >  6:347101/1‑149 (MQ=255)                                                                                                                 gCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTgc                                   <  2:380861/148‑1 (MQ=255)                                                                                                                   aCGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTgcgtc                                >  7:352942/1‑147 (MQ=255)                                                                                                                   aCGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTgcg                                  >  2:231925/1‑147 (MQ=255)                                                                                                                   aCGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGcc                                >  4:369817/1‑149 (MQ=255)                                                                                                                     gAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGcgtcgt                              <  8:352942/149‑1 (MQ=255)                                                                                                                     gAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCgt                              >  2:239753/1‑149 (MQ=255)                                                                                                                      aaGTGGGTTCGAACAACCCTGATGTCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCgtg                             <  3:192687/149‑1 (MQ=255)                                                                                                                           ggTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGgtgt                         <  1:364628/148‑1 (MQ=255)                                                                                                                            gTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGttc                       >  5:393698/1‑149 (MQ=255)                                                                                                                            gTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGttc                       <  4:57777/149‑1 (MQ=255)                                                                                                                            gTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGttc                       <  6:393698/149‑1 (MQ=255)                                                                                                                                 aacaacCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTc                                            <  6:356304/123‑1 (MQ=255)                                                                                                                                 aacaacCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTc                                            >  5:356304/1‑123 (MQ=255)                                                                                                                                     acCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCt              <  5:347101/149‑1 (MQ=255)                                                                                                                                      cccTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCt              >  6:316415/1‑148 (MQ=255)                                                                                                                                       ccTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTg             >  1:341739/1‑148 (MQ=255)                                                                                                                                         tGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGttcttcttc                 <  2:137064/142‑1 (MQ=255)                                                                                                                                         tGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGttcttcttc                 >  1:137064/1‑142 (MQ=255)                                                                                                                                              gCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATggg      <  5:162320/148‑1 (MQ=255)                                                                                                                                               cGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATGGGCg    <  1:106454/149‑1 (MQ=255)                                                                                                                                               cGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATGGGCg    <  1:353722/149‑1 (MQ=255)                                                                                                                                               cGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATGGGCg    >  5:119125/1‑149 (MQ=255)                                                                                                                                                 tCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATGGGCGGt  >  7:268882/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                  TTCGTGGTGACTACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATGGGCGGT  >  NC_002947/1505397‑1505689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑