Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,517,282 G→C 10.1% V158L (GTG→CTG) ‡ murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,517,2820GC10.1% 233.2 / 11.3 77V158L (GTG→CTG) ‡murEUDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base G (32/37);  new base C (4/4);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

CGGCACCAACGGCAAGACAAGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGACCACCTCGAC  >  NC_002947/1517134‑1517428
                                                                                                                                                    |                                                                                                                                                  
cGGCACCAACGGCAAGACAAGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCg                                                                                                                                                    >  2:6587/1‑149 (MQ=255)
         cGGCAAGACAAGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCACGGCAATCgg                                                                                                                                           <  1:409270/149‑2 (MQ=255)
            cAAGACAAGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGAccc                                                                                                                                        <  3:240047/149‑1 (MQ=255)
             aaGACAAGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCACGGCAATCgggtcg                                                                                                                                       <  8:351613/149‑6 (MQ=255)
                 cAAGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAAc                                                                                                                                   <  1:227770/149‑1 (MQ=255)
                  aaGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAAc                                                                                                                                   >  1:150967/1‑148 (MQ=255)
                    gtgtTACCCAACTGGTCGCCCGGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCACGGCAATCgggt                                                                                                                                         <  7:84234/140‑4 (MQ=255)
                    gtgtTACCCAACTGGTCGCCCGGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGACTGACCACGCCCGACCCGATTGCACGGCAATccgga                                                                                                                                         >  8:84234/1‑136 (MQ=255)
                    gtgaTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGAc                                                                                                                                <  6:24106/149‑1 (MQ=255)
                     tgtTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGAc                                                                                                                                >  4:17508/1‑148 (MQ=255)
                       gctttcAACTGGTCGCCCAGGGGCGCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGGCGTGGAATCGACCCTCAACGACCTc                                                                                                                             <  6:224630/144‑1 (MQ=255)
                         aCCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGa                                                                                                                                           <  1:60495/133‑1 (MQ=255)
                         aCCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGa                                                                                                                                           >  2:60495/1‑133 (MQ=255)
                            cAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGa                                                                                                                         >  7:64671/1‑148 (MQ=255)
                               cTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAgg                                                                                                                     <  6:48042/149‑1 (MQ=255)
                                  gTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAggcgg                                                                                                                  >  8:263723/1‑149 (MQ=255)
                                   tCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAggcggc                                                                                                                 >  8:99007/1‑149 (MQ=255)
                                   tCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAggcggc                                                                                                                 >  7:206127/1‑149 (MQ=255)
                                   tCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAggcggc                                                                                                                 >  7:337896/1‑149 (MQ=255)
                                             gcTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCg                                                                                                       >  2:18479/1‑149 (MQ=255)
                                             gcTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCg                                                                                                       >  8:115004/1‑149 (MQ=255)
                                             gcTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCg                                                                                                       >  8:45444/1‑149 (MQ=255)
                                              cTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCTGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGt                                                                                                      <  3:339017/149‑1 (MQ=255)
                                                      gCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATg                                                                                               >  5:346885/1‑148 (MQ=255)
                                                       cTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCAttg                                                                                              <  7:263723/148‑3 (MQ=255)
                                                       cTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATgg                                                                                              <  2:81567/148‑1 (MQ=255)
                                                       cTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATg                                                                                               <  8:64671/147‑1 (MQ=255)
                                                          ggCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGaa                                                                                            <  2:150967/147‑1 (MQ=255)
                                                                      ggCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGc                                                                              <  5:255105/149‑1 (MQ=255)
                                                                       gCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGACCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACACTCAACGACCTCAAGAAAGGCGGCGCCATGGCCGTGGCCATGGAAGTGTCTTCCCATGc                                                                              >  7:27945/1‑148 (MQ=255)
                                                                        ccTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGccc                                                                            >  4:378484/1‑149 (MQ=255)
                                                                         cTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCt                                                                           <  1:6587/149‑1 (MQ=255)
                                                                         cTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAACGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCt                                                                           >  8:171781/1‑149 (MQ=255)
                                                                            aTTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCTTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTg                                                                          <  1:338575/147‑1 (MQ=255)
                                                                            aTTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTg                                                                          <  1:37676/147‑1 (MQ=255)
                                                                            aTTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTg                                                                          >  8:334266/1‑147 (MQ=255)
                                                                             ttGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAg                                                                       <  1:18479/149‑1 (MQ=255)
                                                                                gctcgcTGGGTACGGGTTTCTAGGGCGAGTTGCAGAGCGGCCGGCTGACGACGACCGACCCGTTTGCCGTGCAATCGACCCTCTACGACCTCATGAAAGGCGGCGCCAGGGCCGTGGCCATGTAAGTGTCTTCCCATGCGCTGGAGCaa                                                                    <  8:27945/144‑1 (MQ=255)
                                                                                gCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGc                                                                      >  2:98891/1‑147 (MQ=255)
                                                                                 cACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAg                                                                   <  6:346885/149‑1 (MQ=255)
                                                                                  aCCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAgg                                                                  <  7:334266/149‑1 (MQ=255)
                                                                                        ggTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCgg                                                              <  7:99007/147‑1 (MQ=255)
                                                                                         gTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGTCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGtt                                                           >  1:84230/1‑149 (MQ=255)
                                                                                           aCCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCtt                                                                                     >  3:286622/1‑121 (MQ=255)
                                                                                           aCCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCtt                                                                                     <  4:286622/121‑1 (MQ=255)
                                                                                               gTTTCTACGGCGAGTTGAAGATCGGCCTCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGACATTGAAGTGTCTTCCCATGCCCTGTAGCAAGGCAGGGTTGCCGc                                                      >  8:194453/1‑148 (MQ=255)
                                                                                                        gCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTc                                            <  1:98891/149‑1 (MQ=255)
                                                                                                        gCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTc                                            <  8:337896/149‑1 (MQ=255)
                                                                                                               gCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCACTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATcg                                     >  6:211498/1‑149 (MQ=255)
                                                                                                                cAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAAc                                                                                                                                   >  6:243928/1‑54 (MQ=255)
                                                                                                                cAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAAc                                                                                                                                   <  5:243928/54‑1 (MQ=255)
                                                                                                                 agagCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGCCATcgcg                                   >  3:247438/1‑149 (MQ=255)
                                                                                                                  gagCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCgg                                  >  2:406064/1‑149 (MQ=255)
                                                                                                                        ccgccTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCgg                                  >  1:365256/1‑143 (MQ=255)
                                                                                                                        ccgccTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCgg                                  <  2:365256/143‑1 (MQ=255)
                                                                                                                        ccgccTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGtgatga                            <  3:17508/149‑1 (MQ=255)
                                                                                                                        ccgccTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGtgatga                            <  7:171781/149‑1 (MQ=255)
                                                                                                                           ccTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGAGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTCGAATTCGACATCGCGGTGATGAcc                          >  4:254865/1‑148 (MQ=255)
                                                                                                                            cTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCaa                        <  1:406064/149‑1 (MQ=255)
                                                                                                                               aCCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAAc                       <  7:115004/147‑1 (MQ=255)
                                                                                                                                 cACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGt                   >  6:175995/1‑149 (MQ=255)
                                                                                                                                 cACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGt                   >  6:169822/1‑149 (MQ=255)
                                                                                                                                  aCGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTc                                            <  8:378656/123‑1 (MQ=255)
                                                                                                                                  aCGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTc                                            >  7:378656/1‑123 (MQ=255)
                                                                                                                                      ccgacccgaTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGtgatg                             <  8:254341/134‑1 (MQ=255)
                                                                                                                                      ccgacccgaTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGtgatg                             >  7:254341/1‑134 (MQ=255)
                                                                                                                                         acccgaTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGac           <  6:145253/149‑1 (MQ=255)
                                                                                                                                          cccgaTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGacc          <  7:45444/149‑1 (MQ=255)
                                                                                                                                          cccgaTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATCCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGacc          >  8:369034/1‑149 (MQ=255)
                                                                                                                                           tcgaTTGCACGGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACt                                                   <  4:392043/106‑1 (MQ=255)
                                                                                                                                           tcgaTTGCACGGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACt                                                   >  3:392043/2‑107 (MQ=255)
                                                                                                                                           tcgaTTGCACGGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGacca         >  2:103902/2‑149 (MQ=255)
                                                                                                                                           ccgaTTGCCCTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCCCCAGGGCCGTGGCCATAGAAATGTCTTCCCATGCCCTCCAGCAAGGCCCGGTTGCCGCACTGCAATTCGACATCGCGGTGATGACCAACCAGTCCCGccacca         >  8:358058/1‑149 (MQ=255)
                                                                                                                                            cgaTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGaccac        <  4:247438/149‑1 (MQ=255)
                                                                                                                                              aTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGACCACCt      <  5:169822/149‑1 (MQ=255)
                                                                                                                                              aTTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGACCACCt      <  5:175995/149‑1 (MQ=255)
                                                                                                                                                 gCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGACCACCTCg    <  3:378484/148‑1 (MQ=255)
                                                                                                                                                  ccGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGACCCCCTCGAc  >  4:6665/1‑149 (MQ=255)
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CGGCACCAACGGCAAGACAAGTGTTACCCAACTGGTCGCCCAGGCGCTCGACCTGCTTGGCCAGCGCTGCGGCCTGATTGGCACCCTGGGTACCGGTTTCTACGGCGAGTTGCAGAGCGGCCGCCTGACCACGCCCGACCCGATTGCCGTGCAATCGACCCTCAACGACCTCAAGAAAGGCGGCGCCAGGGCCGTGGCCATGGAAGTGTCTTCCCATGCCCTGGAGCAAGGCCGGGTTGCCGCACTGGAATTCGACATCGCGGTGATGACCAACCTGTCCCGCGACCACCTCGAC  >  NC_002947/1517134‑1517428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑